key enabling technologies for point-of-care diagnostic sensing · key enabling technologies for...
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Key Enabling Technologies for Point-of-Care Diagnostic
SensingWout Knoben - Surfix
Aliki Tsopela – Micronit Microtechnologies
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About Surfix
Our conviction
Micro- and nanotechnology are an integralpart of sustainable solutions for majorglobal challenges in healthcare, food andenergy.
At Surfix, we believe that accurate andprecise control of surface properties willenable our customers to exploit the fullpotential of their world changing devices.
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About Surfix
A nanocoating for everyone• Tailor-made nanocoatings
− substrate material− surface properties− coating architecture− patterning
• Services− surface characterization− coating of devices− technology transfer− close collaboration
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1-2 nm
10-100 nm
3D nanocoating
2D nanocoating
Surfix technology
Nanocoating options• Chemical surface modification
• Covalent attachment of organic molecules
• Coating architectures− 2D− 3D
• Patterning options− uniform− local− material-selective
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material-selective nanocoating
local nanocoating
Surfix technology
Nanocoating options• Chemical surface modification
• Covalent attachment of organic molecules
• Coating architectures− 2D− 3D
• Patterning options− uniform− local− material-selective
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Micronit microtechnologies – key facts
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ISO9001
ISO13485
EnschedeThe Netherlands
Dortmund Germany
1000+we served
customers
50+ countries
in
100+ highlyeducated
multi-disciplinarypeople
founded in
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Micronit microtechnologies - Our core business
Quality manufacturing
• Low-High-volume manufacturing capabilities
Creative product development
• Co-engineering
• Functional microfluidics design
• Design for manufacturing
Research products & prototyping services
• Off-the-shelf standard microfluidic chip modules
• Custom prototyping & low-volume manufacturing
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Any material or combination of materials
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HYBRID
HYBRID
HYBRID
SILICON POLYMERS
GLASS
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Source: Yole Développement
Point-of-Care (PoC) devices
Medical tests at the point of care Market analysis
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Coat PoCKET
Local surface modificationElectrostatically actuated capillary valves
Development of a capillary flow based immunoassay chip
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Point-of-Care (PoC) diagnostics at Micronit
Antibody immobilization
Surfaces passivation
Antigen capture
Detection antibody
Labeling
Detection
Washing
ELISA on chip for the detection of cardiac biomarkers
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Point-of-Care (PoC) diagnostics at Micronit
ELISA on chip for the detection of cardiac biomarkers
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Integrated capillary flow control
Capillary burst valves
• Hot embossing and micromilling of polymers
• Increase in the depth and width of the channel just after the valve
20μm
20μm
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Electrostatic actuation mechanism
• Thin film metal patterning on polymers
• Application of voltage between two electrodes for meniscus triggering
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Integrated capillary flow control
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Integrated capillary flow control
Sequential flow chip
• Multistep assays
• Sequential loading of liquid solutions
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activated COC (bonding)
hydrophilic channel
hydrophobic patch
Coat PoCKET
Coating challenges• patterned coating of COC
• two patterning steps on one substrate
• alignment of coating to− channels− electrodes− previous coating
• compatibility with bonding− surface activation− temperature− solvent
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Patterned coatings on ‘flat’ COC
Single patterning• WCA untreated COC: 95o
• WCA activated COC: 60o (temporary)
• WCA hydrophilic coating: 45o
• WCA hydrophobic coating: 115o
Double patterning• best result obtained
− hydrophobic patch on top of hydrophilic coating− WCA 45o vs. 125o (!)− coatings are compatible with thermal bonding conditions
m
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Patterned coatings in COC channels
Coating of COC channels• alignment of coating with channels/electrodes
• coating in ‘deep’ channels
• hydrophilic coating works well
• hydrophobic coating more difficult to align
Bonding• is the uncoated COC surface still bondable?
• thermal fusion bonding (120 oC, 20 min)
• bonding works well if done on same day as coating400 mm
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Status and outlook
Status• double patterned coating and bonding of COC devices is possible
• encouraging results from first batch of test devices (proof of principle)
• second batch should yield full functionality
Outlook• improve alignment of hydrophobic coating
• development of demonstrator assay
• development of integrated wafer level fabrication process
• Coat PoCKET project runs until the end of 2018
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Concluding remarks
Summary• Capillary flow control and (patterned) surface modification are key
enabling technologies for the next generation PoC diagnostic devices
• Micronit Microtechnologies and Surfix are developing a demonstrator assay to show the potential of the combination of these technologies
More information• Micronit Microtechnologies
− booth− presentation at MicroNanoConference: 13:30 (Veilingzaal)
• Surfix− booth− presentation at MicroNanoConference: 14:00 (Veilingzaal)