www.tuas.fi www.turkuamk.fi

Kari Haajanen

tuntiopettaja, laboratoriopäällikkö

Turun AMK

GMO-ruokaa vai ei?

GMO ja elintarvikkeet

• Realismi

• Idealismi

• Tietoisuus

HS 24.4.2014

Turun AMK 1992–2014

GMO-laboratoriotestin kulku

• Test is based on the detection and amplification of gene regions specific for GMOs (35S promoter and NOS terminator), and not present in native plants.

• 90 % of the GMOs that have been commercialised up to date present this specific regions.

• 35S promoter of the cauliflower mosaic virus. The promoter serves as adocking site for RNA polymerase and a signal for where it should start transcribing a gene.

• Terminator of the nopaline synthase (NOS) gene of Agrobacterium tumefaciens (Gram-negative bacteria). The terminator is the signal to stop transcription.

DN

A:n

eri

stys

DNA:n mittaus:

Suhdeluku A260/A280 1,8 = puhdas DNA

Mittakaavan pienuus aiheuttaa haasteita

PCR-vaiheet

PCR-pipetointi

http://www.dnalc.org/resources/animations/pcr.html

PCR:n idea

PCR:n kopiointikyky on “rajaton”

Agaroosigeelielektroforeesi

http://www.dnalc.org/resources/animations/gelelectrophoresis.html

AGE:n periaate

AGE-tulos

GMO-testin tulos

Luettavaa + materiaalia aiheen tiimoilta:

• First fruit (flavr savr)

• Europeans and biotechnology in 2010

• Biogeeni

• Geenitekniikka

KiitosKysymyksiä?