jack nguyen xiaodong ma 05/14/2008. for today theory/background, testings – jack result, other two...
TRANSCRIPT
Jack Nguyen Xiaodong Ma
05/14/2008
For todayFor todayTheory/Background, Testings – Jack
Result, other two experiments– Xiaodong
OBJECTIVES OF PROJECT:OBJECTIVES OF PROJECT:Fabrication of FP Biosensors Repeated Analyze antigen/antibody (Influenza
A/anti-Influenza A ) interactionsTest different Techniques / Antigens
MATERIALSMATERIALS::Fiber Optic Strands, CTS Machine, Matlab,
PMMA/Acetone solution, Protein G’(10µg/ml), 2% BSA in PBS, Influenza A(20µg/ml), Anti-Influenza A Ig G(20µg/ml), Distilled water, 3-aminopropyltriethoxysilane, Acetone, Ethanol, Microscope, Light, Controllable stage
BudgetBudget: $0, all materials provided by UML
Team RolesTeam RolesTASKS RESPONSIBILITY
Lab Reports / PPTLab Reports / PPT Jack, XiaodongJack, Xiaodong
Fabrication of BiosensorsFabrication of Biosensors JackJack
Lab TestingsLab Testings Jack, XiaodongJack, Xiaodong
Data AnalysisData Analysis XiaodongXiaodong
Schedule FollowedTASKS START END
Sensors Fabrication 4/01/08 5/13/08
Repeat Lab Testings 4/01/08 5/13/08
Present. II/Report 4/09/08 4/16/08
Present.III/Report 4/16/08 5/14/08
Data Analyzation 4/10/08 5/12/08
Test Diff. Antigens 4/30/08 5/13/08
Analyze Diff. Anti. 5/02/08 5/13/08
Finalization All Testings 5/10/08 5/14/08
Principle of FP cavity sensor.
Schematic diagram showing the fringe shift
Pro.G
Influenza A
Primary Antibody
poly(methyl 2-methylpropenoate)
Optical Fiber
Fabrication of Biosensor
SENSOR PMMA PBS
BSA WATER Pro.G
WATER PBS IgG
Inf.A PBS WATER
WATER PBS DATA
Time DurationFabrication FP sensor : 30 MinutesPMMA: 2 HoursPro.G: 1.5 HoursBSA: 1.5 HoursIgG: 1.5 HoursInflu. A: 1.5 HoursTotal PBS: 5 HoursTotal Hours for a typical lab test: 13-16 HrWhen a lab test fails, we start all over with the
FP sensor fabrication
Experimental setup
Chiller
CTS
MatlabProgram
Biomaterials Fiber
Experimental setup
Polymertube
Glasstube
Fiber
Fringes in the air
Fringes in the PBS
Result I without BSA
1537
1539
1541
1543
1545
2:18-3:55p.m. 8:00-8:31p.m. 10:27-10:57p.m. 1:00-1:33a.m.
Pro.G Anti-Inf.A IgG
Inf.A
PBS
Time
Wave Length
Result II without BSA
1540
1542
1544
Wave Length
10:52-11:15a.m. 12:34-1:05p.m. 2:27-3:00p.m. Timeovernight
Pro.G
Anti-Inf.A IgG
Inf.A
PBS
Result III with BSA
1534
1536
1538
1540PBS
Pro.G
Pro.G_PBS
BSA
BSA_PBS
Anti-Inf.A IgGAnti-Inf.A IgG_PBS Inf.A
Inf.A_PBS
1.5hr 1hr 1.5hr 1hr 1.5hr 1hr 1.5hr 1hr0.5hr
Wave Length
Time
Experimental setup
Stage power
Connector to CTS
Detector
Stage
Sensor
Result
Experimental setup
VIS Spectrometer
Detector
Light Source
SiO2/Si
Experimental steps• Wash out the dust of SiO2/Si substrates several times by DI water.• Wash the SiO2/Si substrates by sonication in acetone, ethanol and DI water for 15
min, respetively.• Put the cleaned and dry SiO2/Si substrates in 2% solution of 3-
aminopropyltriethoxysilane in acetone (3-aminopropyltriethoxysilane : acetone =1 : 49 by volume ) at room temperature for 30 seconds.
• Measure the substrates’ spectra by VIS spectrometer after SiO2/Si substrates dried.• Drop Pr.G’ on SiO2/Si substrates for 1.5 h, wash by DI water 3 times, air-dry and
detect its spectra. (For comparison experiment, drop BSA on the substrates first and then wash and detect spectra.)
• Drop anti-Inf.A IgG on the surface for 1.5 h, wash by DI water 3 times, air-dry and test its spectra. (For comparison experiment, drop Pr.G’ on the surface with BSA and wash, dry detect spectra.)
• Drop Inf.A on the surface for 1.5, wash by DI water 3 times, air-dry and then measure its spectra.
• Analyze the spectra of two groups experiment.
Results
0
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
459
479
498
518
537
555
573
591
609
626
643
660
Substrate
Pr.G'
Anti-Inf.A IgG
Inf.A
Relative Intensity
Wavelength (nm)
A
Results
-800-600-400-200
0200400600800
10001200
459
474
489
504
519
533
547
562
576
589
603
616
629
642
655
668
P-S
A-P
I-A
Relative Intensity
Wavelength (nm)
B
Results
0
2000
4000
6000
8000
10000
12000
14000
459
473
486
500
513
526
539
552
565
577
589
602
614
626
637
649
660
Substrate
BSA
Pr.G'
Relative Intensity
Wavelength (nm)
A
Results
-2000
-1500
-1000
-500
0
500
1000
1500
2000
2500
3000
459
473
486
500
513
526
539
552
565
577
589
602
614
626
637
649
660
B-S
P-B
Relative Intensity B
Wavelength (nm)