Is this specific for zebrafish?

Or can we learn how to drive stem cells into specific cell types

in mammals?

Overexpression of mouse Pou5f1 rescues zebrafish embryos mutant for Pou5f1

Evolutionary conservation of function

Overexpression of mouse Pou5f1 rescues zebrafish embryos mutant for Pou5f1

Evolutionary conservation of function

Mouse and zebrafish Pou5f1 share targets:

Comparison with mouse Oct4 target sets(genes with homology info only):Sharov et al, BMC Genomics 2008(Matoba et al., 2006 and Loh et al., 2006)

453 49 406

zebrafish mouse

Relevance?

Insight into controlled differentiation of stem cells to replace lost tissues:

Regenerative medicine

Use knowledge of combination of tissue specific repressors of

differentiation to generate defined cell types

Acknowledgements

& Rebecca Mössner, Kay Kotkamp, Bozena Polock, Björn Wendik, Esther Kur, Bernadette Lippok, Sungmin Song, Heinz-Georg-Belting, Martina Lachnit

Collaborations: Thorsten Kurz (ZBSA Genomics Core)

driever@biologie.uni-freiburg.de

Daria Onichtchouk Florian Geier Jens Timmer (FRIAS)

Onichtchouk et al., Molecular Systems Biology, 6:354 Mar 9 - 2010