ir-4 ornamental horticulture program research report...

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IR-4 Ornamental Horticulture Program Research Report Cover Sheet Researcher(s) Mike Reding Affiliation: USDA-ARS Hort Insects Lab 1680 Madison Avenue Wooster OH 44691 PhoneNumber: (330) 263-3629 Email: [email protected] Trial: 2011 OH 20111104a.pdf ProjectTitle: Spirotetramat Crop Safety PR# Research Target Crop/Plant Product EPA Reg. # Production Site Protocol #: 11-022 Status 29157 Phytotoxicity Phytotoxicity Transvaal Daisy Gerbera sp. Kontos (BYI 8330 240SC) Spirotetramat Field Container 432-1471 OHP C 29158 Phytotoxicity Phytotoxicity Hydrangea Hydrangea sp. Kontos (BYI 8330 240SC) Spirotetramat Field Container 432-1471 OHP C 29159 Phytotoxicity Phytotoxicity Shrub Verbena Lantana sp. Kontos (BYI 8330 240SC) Spirotetramat Field Container 432-1471 OHP C 29160 Phytotoxicity Phytotoxicity Lily Lilium sp. Kontos (BYI 8330 240SC) Spirotetramat Field Container 432-1471 OHP C 29161 Phytotoxicity Phytotoxicity Geranium Pelargonium sp. Kontos (BYI 8330 240SC) Spirotetramat Field Container 432-1471 OHP C 29162 Phytotoxicity Phytotoxicity Rose Rosa sp. Kontos (BYI 8330 240SC) Spirotetramat Field Container 432-1471 OHP C 29164 Phytotoxicity Phytotoxicity Marigold Tagetes sp. Kontos (BYI 8330 240SC) Spirotetramat Field Container 432-1471 OHP C 29165 Phytotoxicity Phytotoxicity Wishbone Flower Torenia sp. Kontos (BYI 8330 240SC) Spirotetramat Field Container 432-1471 OHP C 26974 Phytotoxicity Phytotoxicity Arrowwood Viburnum sp. Kontos (BYI 8330 240SC) Spirotetramat Field Container 432-1471 OHP C 29166 Phytotoxicity Phytotoxicity Pansy Viola sp. Kontos (BYI 8330 240SC) Spirotetramat Field Container 432-1471 OHP C 29167 Phytotoxicity Phytotoxicity Zinnia Zinnia sp. Kontos (BYI 8330 240SC) Spirotetramat Field Container 432-1471 OHP C

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IR-4 Ornamental Horticulture ProgramResearch Report Cover Sheet

Researcher(s) Mike Reding

Affiliation: USDA-ARS

Hort Insects Lab

1680 Madison Avenue

Wooster OH 44691

PhoneNumber: (330) 263-3629

Email: [email protected]

Trial: 2011 OH

20111104a.pdf

ProjectTitle: Spirotetramat Crop Safety

PR# Research Target Crop/Plant Product EPA Reg. # Production Site

Protocol #: 11-022

Status

29157 Phytotoxicity

Phytotoxicity

Transvaal Daisy

Gerbera sp.

Kontos (BYI 8330 240SC)

Spirotetramat

Field Container432-1471

OHP

C

29158 Phytotoxicity

Phytotoxicity

Hydrangea

Hydrangea sp.

Kontos (BYI 8330 240SC)

Spirotetramat

Field Container432-1471

OHP

C

29159 Phytotoxicity

Phytotoxicity

Shrub Verbena

Lantana sp.

Kontos (BYI 8330 240SC)

Spirotetramat

Field Container432-1471

OHP

C

29160 Phytotoxicity

Phytotoxicity

Lily

Lilium sp.

Kontos (BYI 8330 240SC)

Spirotetramat

Field Container432-1471

OHP

C

29161 Phytotoxicity

Phytotoxicity

Geranium

Pelargonium sp.

Kontos (BYI 8330 240SC)

Spirotetramat

Field Container432-1471

OHP

C

29162 Phytotoxicity

Phytotoxicity

Rose

Rosa sp.

Kontos (BYI 8330 240SC)

Spirotetramat

Field Container432-1471

OHP

C

29164 Phytotoxicity

Phytotoxicity

Marigold

Tagetes sp.

Kontos (BYI 8330 240SC)

Spirotetramat

Field Container432-1471

OHP

C

29165 Phytotoxicity

Phytotoxicity

Wishbone Flower

Torenia sp.

Kontos (BYI 8330 240SC)

Spirotetramat

Field Container432-1471

OHP

C

26974 Phytotoxicity

Phytotoxicity

Arrowwood

Viburnum sp.

Kontos (BYI 8330 240SC)

Spirotetramat

Field Container432-1471

OHP

C

29166 Phytotoxicity

Phytotoxicity

Pansy

Viola sp.

Kontos (BYI 8330 240SC)

Spirotetramat

Field Container432-1471

OHP

C

29167 Phytotoxicity

Phytotoxicity

Zinnia

Zinnia sp.

Kontos (BYI 8330 240SC)

Spirotetramat

Field Container432-1471

OHP

C

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

1

Project Title: Phytotoxicity to ornamental horticultural plants from Kontos 240C (spirotetramat) Drench Application Protocol #: 11-022

Narrative Summary (Results/Discussion) Kontos 240 SC (spirotetramat) was applied at the minimum (1x) and maximum (2x) recommended rates, and twice the maximum rate (4x) as a single drench application to 11 ornamental plant species to test for phytotoxicity (Table 1). The treatment at 2 times the maximum rate was used to simulate overlapping applications. Test plants were rated for phytotoxicity 1, 2, 3, 4, 5 and 6 weeks after initial application and measured at the beginning and end of the study to determine differences in growth. Drench volumes to wet soil media without loss of solution from bottom of pots were: 50 ml/4” pots, 75 ml/6” pots and 100 ml/8”pots Treatments Untreated control 1x = 3.4 fl oz per 1500 4 inch pots or 0.0023 oz/4” pot 2x = 6.8 fl oz per 1500 4 inch pots or 0.0046 oz/4” pot 4x = 13.6 fl oz per 1500 4 inch pots or 0.0091 oz/4” pot Ten species showed no phytotoxicity or significant differences in growth between treatments and were marketable at the conclusion of the study. See table 1 on page 2. Phytotoxicity occurred on zonal geranium (Pelargonium sp.), which exhibited bleached foliage with necrotic leaf margins on the new growth, stunted leaves and reduced flowering with significant differences between untreated and 1x treatments and 2x and 4x treatments. There was a significant difference in height between treatments (Table 2 and Fig. 1 on page 3).

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

2

Results Tables Table 1. PR# Plant Species Phytotoxicity and Growth results 29157 Transvaal Daisy / Gerbera

sp. No injury and no significant difference in growth or marketability.

29158 Hydrangea/ Hydrangea anomala petiolaris

No injury and no significant difference in growth or marketability.

29159 Shrub Verbena / Lantana sp. No injury and no significant difference in growth or marketability.

29160 Lily /Lilium superbum No injury and no significant difference in growth or marketability.

29162 Rose / Rosa rugosa No injury and no significant difference in growth or marketability.

29164 Marigold / Tagetes sp. No injury and no significant difference in growth or marketability.

29165 Wishbone Flower / Torenia sp.

No injury and no significant difference in growth or marketability.

26974 Arrowwood / Viburnum dentatum ‘Chicago Lustre’

No injury and no significant difference in growth or marketability.

29166 Pansy / Viola sp. No injury and no significant difference in growth or marketability.

29167 Zinnia / Zinnia sp. No injury and no significant difference in growth or marketability.

29161 Geranium / Pelargonium sp. Significant injury to foliage and significant differences in flowering and growth. Treated plants not marketable.

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

3

Table 2. Phytotoxicity ratings (Index of injury: 0=no injury, 10=complete kill) taken on 6 dates, 1, 2, 3, 4, 5 and 6 weeks and flowering ratings (0=flowering, 10 = no flowers) taken 6 weeks after the drench applications of Kontos 240SC (spirotetramat) applied to Geranium (Pelargonium sp., PR# 29161). No phytotoxicity occurred until the third rating date, 25 May. Treatment rate Mean Phytotoxicity Rating Mean Flowering Mean (ai/A) 25 May 1 June 8 June 15 June 15 June Height (in) Untreated 0.0a 0.0a 0.0a 0.0a 0.0a 1.9a 3.4 fl oz/1500 4” pots 0.0a 0.1a 0.1a 0.2a 0.0a 1.1bc 6.8 fl oz/1500 4” pots 1.4b 3.0b 3.2b 3.2b 6.7b 1.6ab 13.6fl oz/1500 4” pots 1.3b 2.9b 3.1b 3.4b 6.7b 0.7c F 7.01 7.8 27.3 24.8 9.6 8.4 df 3,6 3,6 3,6 3,6 3,6 3,6 P 0.02 <0.02 <0.01 <0.01 0.01 0.01 Means within columns followed by the same letter are not significantly different ANOVA (P = 0.05), means separated by LSD (α = 0.05).

Materials & Methods/Recordkeeping Crop History:

Crop Cultivar/Variety: See table 1 on page 2.

Date of Seeding: N/A

Date of Emergence: N/A

April 15 to April 28

3 weeks after drench application of Kontos

Untreated 4X drench

Fig. 1

2X drench

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

4

Date of Transplanting:

Potting Mix:

Mixture of sphagnum peat (70%), perlite (20%), and

vermiculite (10%)

Pot size & spacing:

4 inch, 6inch and 8” pots (depending on transplant size),

6 inch spacing

Row spacing: 1 ft.

Product(s) applied prior to start of experiment:

Product Rate Application Type

Date of Application

Crop Growth Stage

Application Volume

Peters soluble 20-10-20

188 ppm Watering can 4/15/2011 – 4/28/2011

New transplant

1 teaspoon per gallon of water

Osmocote slow release 13-13-13

1 Tbs. per 1 gal. pot

Sprinkled on soil surface

4/28/2011 new transplant 1 Tbs. per 1 gal. pot

Experiment Information:

Experimental Design: Randomized complete block

Number of Reps: 3 replications (3 plants per replicate)

Materials & Methods: Three rates of Kontos 240C (spirotetramat) were applied as a single drench to 11 ornamental plant species to test for phytotoxicity. Plants were purchased in 2” pots and transplanted into 4” pots (geranium, zinnia, lily, marigold, lantana, viola, and wishbone flower) or 4” pots transplanted into 6” pots (Transvaal daisy) or bare root (hydrangea, rose and arrowwood) and transplanted into 8” pots between 15 April and 28 April, 2011. The potting media was a mixture of sphagnum peat (70%), perlite (20%), and vermiculite (10%). After transplanting, the containers were fertilized with Peters soluble 20-10-20 and put in a polyhouse until temperatures warmed outdoors. Plants were arranged in a randomized complete block design with 3 replications of 3 plants per treatment. The plastic covering was removed from the house on 2 May. Plants were irrigated with a hand wand as needed. Application Equipment: Drench solution poured by hand from graduated cylinder.

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

5

Product(s) applied during experiment (including treatments, fertilizers, etc):

Product Rate(s) Application Type

Date of Application

Crop Growth Stage

Application Volume

Kontos (BYI-8330)

1x = 3.4 fl oz/1500 4” pots 2x = 6.8 fl oz/1500 4” pots 4x =13.6 fl oz/1500 4” pots

Drench solution poured over soil surface

5/4/2011 vegetative to flowering

50ml/4” pot 75ml/6” pot 100ml/8” pot

Kontos (BYI-8330)

1x = 3.4 fl oz/1500 4” pots 2x = 6.8 fl oz/1500 4” pots 4x =13.6 fl oz/1500 4” pots

Drench solution poured over soil surface

5/18/2011 vegetative to flowering

50ml/4” pot 75ml/6” pot 100ml/8” pot

Kontos (BYI-8330)

1x = 3.4 fl oz/1500 4” pots 2x = 6.8 fl oz/1500 4” pots 4x =13.6 fl oz/1500 4” pots

Drench solution poured over soil surface

6/1/2011 vegetative to flowering

50ml/4” pot 75ml/6” pot 100ml/8” pot

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

6

Environmental conditions during the experiment: 2011 Weather Data- WOOSTER, OHIO

April DATE Precipitation (inches) Max Air Temp. (°F) Min Air Temp. (°F) Average Relative Humidity (%)

4/1 0 48.3 31.3 69

4/2 0.16 46.8 33.6 85

4/3 0 48.8 28.4 78

4/4 1.2 67.3 43.0 81

4/5 0.03 43.1 33.2 77

4/6 0 30.6 31.9 70

4/7 0 51.8 39.7 85

4/8 0.07 50.9 44.1 90

4/9 0 55.7 42.6 90

4/10 0 83.2 42.7 78

4/11 0.13 74.5 53.9 78

4/12 0.09 53.7 42.2 75

4/13 0 59.5 36.0 53

4/14 0 64.3 33.5 66

4/15 0 69.8 42.7 61

4/16 0.67 64.6 40.0 85

4/17 0.01 56.8 37.7 63

4/18 0 52.4 39.9 77

4/19 0.45 60.2 38.8 95

4/20 0.38 64.3 41.4 85

4/21 0 50.8 37.4 69

4/22 0.13 53.5 38.7 81

4/23 0.07 70.6 53.5 87

4/24 0.39 59.5 52.8 96

4/25 0.44 72.2 53.9 93

4/26 0.06 76.6 55.8 80

4/27 0.17 77.0 57.6 80

4/28 0.06 64.8 46.2 74

4/29 0.04 56.8 41.4 76

4/30 0 62.3 33.5 74

Totals/Mean 4.55 59.69 41.58 78.37

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

7

May 2011 DATE Precipitation (inches) Max Air Temp. (°F) Min Air Temp. (°F) Average Relative Humidity (%)

5/1 0.21 67.8 52.3 81

5/2 0.31 62.4 47.5 88

5/3 0.76 47.5 43.3 93

5/4 0.02 51.7 39.1 79

5/5 0 61.9 34.8 66

5/6 0.01 60.4 41.0 77

5/7 0.03 66.9 45.5 78

5/8 0 67.2 47.1 74

5/9 0 69.2 38.9 64

5/10 0 76.8 50.1 58

5/11 0 84.4 52.9 65

5/12 0.74 82.7 60.9 86

5/13 1.27 82.0 60.5 87

5/14 0.33 77.9 59.7 91

5/15 0.4 62.4 45.7 96

5/16 0.03 47.6 43.7 93

5/17 0.17 51.0 44.9 95

5/18 0.01 67.0 51.1 92

5/19 0 65.7 52.4 86

5/20 0 73.9 55.2 80

5/21 0 79.6 48.1 76

5/22 0 84.5 62.4 72

5/23 0.40 79.7 63.0 83

5/24 1.28 77.4 61.4 87

5/25 0.70 80.4 59.6 89

5/26 0.59 75.8 59.0 88

5/27 0.01 70.1 57.3 90

5/28 0 77.1 55.6 81

5/29 0 87.0 63.3 77

5/30 0 89.4 65.2 76

5/31 0 90.7 65.6 79

Totals/Mean 7.27 71.55 52.49 81.52

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

8

JUNE 2011

DATE Precipitation (inches) Max Air Temp. (°F) Min Air Temp. (°F) Average Relative Humidity (%)

6/1 0 83.1 66.6 61

6/2 0 70.3 52.8 62

6/3 0 78.0 42.8 63

6/4 0.12 89.2 53.1 75

6/5 0.45 84.7 62.7 74

6/6 0 86.5 56.4 70

6/7 0.72 78.8 62.8 82

6/8 0 91.5 64.3 77

6/9 0.02 85.3 68.3 74

6/10 0.51 81.5 62.0 87

6/11 0.02 82.7 62.2 85

6/12 0 69.8 51.6 87

6/13 0 73.5 49.4 74

6/14 0 68.3 50.2 78

6/15 0 73.2 49.7 77

6/16 0.01 76.7 59.5 82

6/17 0.02 81.9 56.7 81

6/18 0.01 83.2 62.6 84

6/19 0.29 80.8 63.6 89

6/20 0.03 76.1 60.2 94

6/21 0.67 87.2 66.6 87

6/22 0.01 79.8 68.7 85

6/23 0.13 77.2 64.9 85

6/24 0.11 67.8 60.0 91

6/25 0 71.0 60.8 81

6/26 0 78.3 56.8 77

6/27 0 80.6 53.3 80

6/28 0 81.2 64.9 77

6/29 0 76.7 55.6 72

6/30 0 83.8 52.7 74

Totals/Mean 3.12 79.29 58.73 78.83

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

9

July 2011 DATE Precipitation (inches) Max Air Temp. (°F) Min Air Temp. (°F) Average Relative Humidity (%)

7/1 0 88.4 57.2 71

7/2 0.23 83.5 68.3 84

7/3 0 86.2 64.5 77

7/4 0 86.2 62.7 78

7/5 0 87.1 56.4 68

7/6 0 88.8 60.4 73

7/7 0 86.1 64.2 75

7/8 0.75 80.3 61.1 82

7/9 0 88.4 55.2

73

7/10 0 89.0 60.9 72

7/11 0.73 87.7 67.3 89

7/12 0 88.0 68.0 81

7/13 0 77.3 61.8 77

7/14 0 80.2 57.1 74

7/15 0 86.0 58.5 76

7/16 0 85.8 63.0 75

7/17 0 88.0 64.9 83

7/18 0.16 89.4 68.8 88

7/19 0.04 88.5 71.3 87

7/20 0 92.9 67.6 81

7/21 0 93.9 72.8 79

7/22 0.27 95.0 71.6 83

7/23 0.65 90.3 71.4 88

7/24 0.08 88.8 71.3 87

7/25 0 85.8 68.4 84

7/26 0.01 85.2 65.1 79

7/27 0 85.1 59.7 74

7/28 0.01 90.7 70.8 79

7/29 0 87.4 72.6 84

7/30 0 88.2 67.2 75

7/31 0 89.1 61.1 74

Totals/Mean 2.93 87.33 66.42 79.03

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

10

Data Collected: Application date: 5/4/2011 Phytotoxicity Rating Dates: 5/11/2011, 5/18/2011, 5/25/2011, 6/1/2011, 6/8/2011, and 6/15/2011. Phytotoxicity rated: 0=NO INJURY, 10=COMPLETE KILL Phytotoxicity ratings were recorded 1, 2, 3, 4, 5, and 6 weeks after the first foliar application of Kontos. Height and width measurements were taken before and after the tests: 5/4/2011 and 6/17/2011. Statistix 8.1 was used to analyze data. Files are saved in word documents. Raw Data All raw data is saved in a file “Kontos drench 2011” on CD Data: measurements, phytotoxicity, photographs and statistics Weather data was obtained from the weather station on the Wooster, Ohio OARDC campus and can be accessed from the following web site: http://www.oardc.ohio-state.edu/newweather/stationinfo.asp?id=1

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

1

Project Title: Phytotoxicity to ornamental horticultural plants from Kontos 240C (spirotetramat) Foliar Application Protocol #: 11-022

Narrative Summary (Results/Discussion) Kontos 240 SC (spirotetramat) was applied at the minimum (1x) and maximum (2x) recommended rates, and twice the high rate (4x) as foliar sprays to 11 ornamental plant species to test for phytotoxicity (Table 1). Three applications were made at 2 week intervals. The 2 times maximum rate treatment was used to simulate overlapping applications. A surfactant, Top Surf, was added to the spray solutions at a rate of 2 pints per 100 gallons solution. Test plants were rated for phytotoxicity 1, 2, 3, 4, 5 and 6 weeks after initial application and measured at the beginning and end of the study to compare growth among treatments. The test area was 18 sq. ft. with each treatment area equal to 4.5 sq. ft. Treatments Untreated control 1x = 3.4 fl oz per 100 gal = 100.6ml per 100 gal. = 0.27ml / liter 2x = 6.8 fl oz per 100 gal = 201ml per 100 gal. = 0.53ml / liter 4x = 13.6 fl oz per 100 gal = 402ml per 100 gal. = 1.06ml / liter Ten species showed no phytotoxicity or significant differences in growth between treatments and were marketable at the conclusion of the study. See table 1 on page 2. Phytotoxicity occurred on zonal geranium (Pelargonium sp.), which exhibited bleached foliage with necrotic leaf margins on the new growth, stunted leaves and reduced flowering with significant differences between untreated and 1x treatments and 2x and 4x treatments (Table 2 and Fig. 1 on page 3). There were no significant differences in growth between treatments.

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

2

Results Tables Table 1. PR# Plant Species Phytotoxicity and Growth results 29157 Transvaal Daisy / Gerbera sp. No injury and no significant difference in growth or

marketability. 29158 Hydrangea/ Hydrangea

anomala petiolaris No injury and no significant difference in growth or marketability.

29159 Shrub Verbena / Lantana sp. No injury and no significant difference in growth or marketability.

29160 Lily /Lilium superbum No injury and no significant difference in growth or marketability.

29162 Rose / Rosa rugosa No injury and no significant difference in growth or marketability.

29164 Marigold / Tagetes sp. No injury and no significant difference in growth or marketability.

29165 Wishbone Flower / Torenia sp.

No injury and no significant difference in growth or marketability.

26974 Arrowwood / Viburnum dentatum ‘Chicago Lustre’

No injury and no significant difference in growth or marketability.

29166 Pansy / Viola sp. No injury and no significant difference in growth or marketability.

29167 Zinnia / Zinnia sp. No injury and no significant difference in growth or marketability.

29161 Geranium / Pelargonium sp. Significant injury to foliage and significant differences in flowering. Treated plants not marketable.

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

3

Table 2. Phytotoxicity ratings (Index of injury: 0=no injury, 10=complete kill) taken on 6 dates, 1, 2, 3, 4, 5 and 6 weeks and flowering ratings (0=flowering, 10 = no flowers) taken on weeks 5 and 6 after the initial foliar applications of Kontos 240SC (spirotetramat) applied to Geranium (Pelargonium sp., PR# 29161). Treatment rate Mean Phyto Rating Mean Flowering (ai/A) 11 May 18 May 25 May 1 June 8 June 15 June 8 June 15 June Untreated 0.0 0.0 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 3.4 fl oz/100gal 0.0 0.0 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 6.8 fl oz/100gal 0.0 0.0 1.6b 2.9b 2.5b 2.9b 5.5b 7.8b 13.6fl oz/100gal 0.0 0.0 1.1ab 2.8b 2.7b 2.7b 1.1a 5.6ab F 6.05 7.5 12.0 23.6 6.1 4.3 df 3,6 3,6 3,6 3,6 3,6 3,6 P 0.03 0.02 <0.01 0.001 0.03 0.06 Means within columns followed by the same letter are not significantly different ANOVA (P = 0.05), means separated by LSD (α = 0.05).

3 weeks after 1st application of Kontos

Untreated

4X foliar

Fig. 1

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

4

Materials & Methods/Recordkeeping Crop History:

Crop Cultivar/Variety: See table 1 on page 2.

Date of Seeding: N/A

Date of Emergence: N/A

Date of Transplanting: April 15 to April 28

Potting Mix:

Mixture of sphagnum peat (70%), perlite (20%), and

vermiculite (10%)

Pot size & spacing:

4 inch, 6inch and 8” (depending on transplant size),

6 inch spacing

Row spacing: 1 ft.

Product(s) applied prior to start of experiment:

Product Rate Application Type

Date of Application

Crop Growth Stage

Application Volume

Peters soluble 20-10-20

188 ppm Watering can 4/15/2011 – 4/28/2011

New transplant

1 teaspoon per gallon of water

Osmocote slow release 13-13-13

1 Tbs. Per 1 gal. pot

Sprinkled on soil surface

4/28/2011 new transplant 1 Tbs. Per 1 gal. pot

Experiment Information:

Experimental Design: Randomized complete block

Number of Reps: 3 replications (3 plants per replicate)

Materials & Methods: Three rates of Kontos 240C (spirotetramat) were applied as foliar sprays to 11 ornamental plant species to test for phytotoxicity. Three foliar applications were made at 2 week intervals. Plants were purchased in 2” pots and transplanted into 4” pots (geranium, zinnia, lily, marigold, lantana, viola, and wishbone flower) or 4” pots transplanted into 6” pots (Transvaal daisy) or bare root (hydrangea, rose and arrowwood) and transplanted into 8” pots between 15 April and 28 April, 2011. The potting media was a mixture of sphagnum peat (70%), perlite (20%), and vermiculite (10%). After transplanting, the containers were fertilized with Peters soluble 20-10-20 and put in a polyhouse until temperatures warmed outdoors. Plants were

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

5

arranged in a randomized complete block design with 3 replications of 3 plants per treatment. The plastic covering was removed from the house on 2 May. Plants were irrigated with a hand wand as needed. Application Equipment: Hand sprayer Product(s) applied during experiment (including treatments, fertilizers, etc):

Product Rate(s) Application Type

Date of Application

Crop Growth Stage

Application Volume

Kontos (BYI-8330)

1x = 3.4 fl oz/100gal + Top Surf surfactant 2x = 6.8 fl oz/100gal + Top Surf surfactant 4x =13.6 fl oz/100gal + Top Surf surfactant

hand sprayer 5/4/2011 vegetative to flowering

100 gal / A

Kontos (BYI-8330)

1x = 3.4 fl oz/100gal + Top Surf surfactant 2x = 6.8 fl oz/100gal + Top Surf surfactant 4x =13.6 fl oz/100gal + Top Surf surfactant

hand sprayer 5/18/2011 vegetative to flowering

100 gal / A

Kontos (BYI-8330)

1x = 3.4 fl oz/100gal + Top Surf surfactant 2x = 6.8 fl oz/100gal + Top Surf surfactant 4x =13.6 fl oz/100gal + Top Surf surfactant

hand sprayer 6/1/2011 vegetative to flowering

100 gal / A

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

6

Environmental conditions during the experiment: 2011 Weather Data- WOOSTER, OHIO

April DATE Precipitation (inches) Max Air Temp. (°F) Min Air Temp. (°F) Average Relative Humidity (%)

4/1 0 48.3 31.3 69

4/2 0.16 46.8 33.6 85

4/3 0 48.8 28.4 78

4/4 1.2 67.3 43.0 81

4/5 0.03 43.1 33.2 77

4/6 0 30.6 31.9 70

4/7 0 51.8 39.7 85

4/8 0.07 50.9 44.1 90

4/9 0 55.7 42.6 90

4/10 0 83.2 42.7 78

4/11 0.13 74.5 53.9 78

4/12 0.09 53.7 42.2 75

4/13 0 59.5 36.0 53

4/14 0 64.3 33.5 66

4/15 0 69.8 42.7 61

4/16 0.67 64.6 40.0 85

4/17 0.01 56.8 37.7 63

4/18 0 52.4 39.9 77

4/19 0.45 60.2 38.8 95

4/20 0.38 64.3 41.4 85

4/21 0 50.8 37.4 69

4/22 0.13 53.5 38.7 81

4/23 0.07 70.6 53.5 87

4/24 0.39 59.5 52.8 96

4/25 0.44 72.2 53.9 93

4/26 0.06 76.6 55.8 80

4/27 0.17 77.0 57.6 80

4/28 0.06 64.8 46.2 74

4/29 0.04 56.8 41.4 76

4/30 0 62.3 33.5 74

Totals/Mean 4.55 59.69 41.58 78.37

Field Code Changed

Formatted Table

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

7

May 2011 DATE Precipitation (inches) Max Air Temp. (°F) Min Air Temp. (°F) Average Relative Humidity (%)

5/1 0.21 67.8 52.3 81

5/2 0.31 62.4 47.5 88

5/3 0.76 47.5 43.3 93

5/4 0.02 51.7 39.1 79

5/5 0 61.9 34.8 66

5/6 0.01 60.4 41.0 77

5/7 0.03 66.9 45.5 78

5/8 0 67.2 47.1 74

5/9 0 69.2 38.9 64

5/10 0 76.8 50.1 58

5/11 0 84.4 52.9 65

5/12 0.74 82.7 60.9 86

5/13 1.27 82.0 60.5 87

5/14 0.33 77.9 59.7 91

5/15 0.4 62.4 45.7 96

5/16 0.03 47.6 43.7 93

5/17 0.17 51.0 44.9 95

5/18 0.01 67.0 51.1 92

5/19 0 65.7 52.4 86

5/20 0 73.9 55.2 80

5/21 0 79.6 48.1 76

5/22 0 84.5 62.4 72

5/23 0.40 79.7 63.0 83

5/24 1.28 77.4 61.4 87

5/25 0.70 80.4 59.6 89

5/26 0.59 75.8 59.0 88

5/27 0.01 70.1 57.3 90

5/28 0 77.1 55.6 81

5/29 0 87.0 63.3 77

5/30 0 89.4 65.2 76

5/31 0 90.7 65.6 79

Totals/Mean 7.27 71.55 52.49 81.52

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

8

JUNE 2011 DATE Precipitation (inches) Max Air Temp. (°F) Min Air Temp. (°F) Average Relative Humidity (%)

6/1 0 83.1 66.6 61

6/2 0 70.3 52.8 62

6/3 0 78.0 42.8 63

6/4 0.12 89.2 53.1 75

6/5 0.45 84.7 62.7 74

6/6 0 86.5 56.4 70

6/7 0.72 78.8 62.8 82

6/8 0 91.5 64.3 77

6/9 0.02 85.3 68.3 74

6/10 0.51 81.5 62.0 87

6/11 0.02 82.7 62.2 85

6/12 0 69.8 51.6 87

6/13 0 73.5 49.4 74

6/14 0 68.3 50.2 78

6/15 0 73.2 49.7 77

6/16 0.01 76.7 59.5 82

6/17 0.02 81.9 56.7 81

6/18 0.01 83.2 62.6 84

6/19 0.29 80.8 63.6 89

6/20 0.03 76.1 60.2 94

6/21 0.67 87.2 66.6 87

6/22 0.01 79.8 68.7 85

6/23 0.13 77.2 64.9 85

6/24 0.11 67.8 60.0 91

6/25 0 71.0 60.8 81

6/26 0 78.3 56.8 77

6/27 0 80.6 53.3 80

6/28 0 81.2 64.9 77

6/29 0 76.7 55.6 72

6/30 0 83.8 52.7 74

Totals/Mean 3.12 79.29 58.73 78.83

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

9

July 2011 DATE Precipitation (inches) Max Air Temp. (°F) Min Air Temp. (°F) Average Relative Humidity (%)

7/1 0 88.4 57.2 71

7/2 0.23 83.5 68.3 84

7/3 0 86.2 64.5 77

7/4 0 86.2 62.7 78

7/5 0 87.1 56.4 68

7/6 0 88.8 60.4 73

7/7 0 86.1 64.2 75

7/8 0.75 80.3 61.1 82

7/9 0 88.4 55.2

73

7/10 0 89.0 60.9 72

7/11 0.73 87.7 67.3 89

7/12 0 88.0 68.0 81

7/13 0 77.3 61.8 77

7/14 0 80.2 57.1 74

7/15 0 86.0 58.5 76

7/16 0 85.8 63.0 75

7/17 0 88.0 64.9 83

7/18 0.16 89.4 68.8 88

7/19 0.04 88.5 71.3 87

7/20 0 92.9 67.6 81

7/21 0 93.9 72.8 79

7/22 0.27 95.0 71.6 83

7/23 0.65 90.3 71.4 88

7/24 0.08 88.8 71.3 87

7/25 0 85.8 68.4 84

7/26 0.01 85.2 65.1 79

7/27 0 85.1 59.7 74

7/28 0.01 90.7 70.8 79

7/29 0 87.4 72.6 84

7/30 0 88.2 67.2 75

7/31 0 89.1 61.1 74

Totals/Mean 2.93 87.33 66.42 79.03

IR-4 Ornamental Horticulture Program Research Report Form

Researcher(s): Betsy Anderson and Mike Reding

PRNumber(s): 29157, 29158, 29159, 29160, 29162, 29164, 29165, 26974, 29166, 29167, 29161

10

Data Collected: Application dates: 5/4/2011, 5/18/2011, and 6/1/2011. Phytotoxicity Rating Dates: 5/11/2011, 5/18/2011, 5/25/2011, 6/1/2011, 6/8/2011, and 6/15/2011. Phytotoxicity rated: 0=NO INJURY, 10=COMPLETE KILL Phytotoxicity ratings were recorded 1, 2, 3, 4, 5, and 6 weeks after the first foliar application of Kontos. Height and width measurements were taken before and after the tests: 5/4/2011 and 6/17/2011. Statistix 8.1 was used to analyze data. Files are saved in word documents. Raw Data All raw data is saved in a file “Kontos Foliar 2011” on CD Data: measurements, phytotoxicity, photographs and statistics Weather data was obtained from the weather station on the Wooster, Ohio OARDC campus and can be accessed from the following web site: http://www.oardc.ohio-state.edu/newweather/stationinfo.asp?id=1