Introduction to

The Genomics Sciences Lab Services and Support

The GSL operates as a solution center and provides CHHE and the NCSU community, collaborators, and the scientific community with access to HTS, genotyping and support services

In addition to serving as a research resource, the GSL is committed to the education and training of CHHE students, faculty, and staff.

GSL General Mission

GSL MISSION for CHHE

High-throughput sequencing technology at NCSU GSL will support CHHE researchers to conduct • Complete transcriptome analysis Transcriptome development Gene expression analysis)• ChIPseq• Gene mutation analysis• SNP analysis• DNA methylation • miRNA analysis• eQTL analysis• Genotyping by Sequencing • Genome wide association studies

AIM 1. Provide expertise in study design and sample preparation. The Genomics Section will provide guidance in all areas of high-throughput sequencing services, including assistance with sample and project preparation, and implementation of protocols for sequencing on various platforms.

AIM 2. Provide high-throughput sequencing services. The Genomics Section will provide high-throughput sequencing technology and instrumentation, library preparation, and consultation for all levels of experimental design, development, and assessment.

AIM 3. Provide guidance and expertise on Big Data analysis. Provide guidance and expertise on Big Data analysis. The GSL is linked to NC State’s Bioinformatics Research Center (BRC) and large dataset management and analysis will be conducted at the BRC by coordinating with the STC Bioinformatician when needed.

GSL AIMS for CHHE

Support from start to finish – AIMS 1-3

Experiment design

Experiment execution

Tissue collection

Nucleic acid extractionNucleic acid QC

Library synthesis

Sequencing

Data QC

Data analysis

Publication

Experiment design

Experiment execution

Tissue collection

Nucleic acid extractionNucleic acid QC

Library synthesis

Sequencing

Data QC

Data analysis

Publication

Choosing the best platform(s), pooling and lane use

Watch and learn, FREE for CHHE - TruSeq, RiboZero, NebNext etc. Low input, Ultra low input

Support from start to finish – AIMS 1-3

http://research.ncsu.edu/gsl

AIM 2

AIM 2

+ NextSeq500!!Coming soon!

HiSeq

MiSeq

PacBio

These are cartoons of reads!!

Choosing the best platform – AIM 1

HiSeq

MiSeq

PacBio

Choosing the best platform – AIM 1

HiSeq

MiSeq

PacBio

Error typeLow complexity/diversity

AccuracyStarting quantity

Choosing the best platform – AIM 1

Sanger Sequencing

Is your tube Single and alone?Bring it to us!

For the LOW price of $4.00 your tube can be SEQUENCED and finally find a home!

*full plates too!*free pick-up service*data delivered 4-5pm

Other equipment and services

http://research.ncsu.edu/gsl