introduction to biomems & medical microdevices, bmen 5151 · dielectrophoretic separation of...
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Introduction to BioMEMS & Medical Microdevices, BMEn 5151
Course Introduction 2020 Prof. Steven S. Saliterman, http://saliterman.umn.edu/
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Steven S. Saliterman
Topics
1) Nano- and Microfabrication of Silicon & Polymers. 2) Microfluidics - Design, Transport, and Electrokinetics. 3) Biosensors, Microsensors and Nanotechnology. 4) Lab, Organ and Body-on-a-Chip Systems. 5) Microactuators & Drug Delivery. 6) Clinical Laboratory Medicine & Micro Total Analysis Systems. 7) Genomics and Proteomics - Gene and Protein Chips. 8) Clinical Applications & Point-of-Service Devices. 9) Biocompatibility, FDA & ISO 10993. 10) Packaging, Power, Data & RF Safety.
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Steven S. Saliterman
Nano- and Micro Realm
US Department of Energy, Office of Science 2019
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Steven S. Saliterman
BioMEMS…
Biomedical Micro Electro-Mechanical Systems. Devices or systems, constructed from nano or
microfabrication, that are used for processing, delivery, manipulation, analysis or construction of biological and chemical materials.
At least one dimension is from ~100 nm to 200 µm. Incorporating new materials and an understanding of the
nano- microenvironment, and biocompatibility. Harnessing any phenomenon that accomplish work at the
microscale. Includes research and laboratory tools, and point-of-
service, therapeutic and implantable devices.
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Steven S. Saliterman
Silicon Nano- and Microfabrication
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Steven S. Saliterman
Nano-Bio Lab Facility…
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Steven S. Saliterman
Polymer Microfabrication
Image courtesy of Jaehoon Chung & Euisik Yoon
Image courtesy of Bertsch A. Image courtesy of Marco, CD
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Steven S. Saliterman
Microfluidics
Science of fluid behavior in microchannels. In lab-on-a-chip and µTAS devices, the following
features are often seen: Microchannels, Microfilters, Microvalves, Micropumps, Microneedles, Microreserviors, Micro-reaction chambers.
Image courtesy of Micronit
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Steven S. Saliterman
Rapid Prototyping Systems in PDMS…
Image courtesy of Sylgard
Duffy DC, McDonald JC, Schueller OJA, Whitesides GM. Rapid prototyping of microfluidic systems in poly(dimethylsiloxane). Analytical Chemistry. 1998;70(23):4974-4984.
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Steven S. Saliterman
Large-Scale Integration…
1 2 3 4 5 6 7 8 9 10 11 16 15 14 13 12
Left) Melin J, Quake SR. Microfluidic large-scale integration: The evolution of design rules for biological automation. In: Annual Review of Biophysics and Biomolecular Structure. Vol 36.2007:213-231. Right) Liu J, Hansen C, Quake SR. 2003. Solving the “world-to-chip” interface problem with a microfluidic matrix. Anal. Chem. 75(18):4718–23
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Steven S. Saliterman
Electrokinetics…
Electrokinetic phenomenon: Electroosmosis Electrophoresis Dielectrophoresis
An important tool for moving, separating and concentrating fluid and suspended particles.
Hossan MR, Dutta D, Islam N, Dutta P. Review: Electric field driven pumping in microfluidic device. Electrophoresis. 2018;39(5-6):702-731.
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Steven S. Saliterman
Transport Processes…
Fluid Mechanics: Laminar flow, Fluid kinematics.
Mixing by diffusion, special geometries and mechanical means.
Effects of increased surface area-to- volume as dimensions are reduced in microfluidic channels.
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Steven S. Saliterman
Biosensors & Nanotechnology
Luka G, Ahmadi A, Najjaran H, et al. Microfluidics Integrated Biosensors: A Leading Technology towards Lab-on-a-Chip and Sensing Applications. Sensors. 2015;15(12):30011-30031.
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Steven S. Saliterman
Microsensors…
Kottapalli AGP, Shen Z, Asadnia M, et al. Polymer MEMS sensor for flow monitoring in biomedical device applications. 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS); 22-26 Jan. 2017, 2017.
Dinh T, Phan H, Qamar A, et al. Environment-friendly wearable thermal flow sensors for noninvasive respiratory monitoring. 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS); 22-26 Jan. 2017, 2017.
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Steven S. Saliterman
Nanotransducers…
Nanoparticle transducers: Quantum dots. Carbon dots. Nobel metal nanoparticles. Lanthanide nanoparticles.
Label free transducers - rather than relying on attachment to reporter labels for signal transduction: Nanowires Nanotubes Nanocantilevers Mesoporous membranes.
Hildebrandt N, Spillmann CM, Algar WR, et al. Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications. Chemical Reviews. 2017;117(2):536-711.
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Steven S. Saliterman
Lab-on-a-Chip
Tran, S.B.Q., Marmottant, P., Thibault, P., 2012. Appl. Phys. Lett., 101.
Jubery, TZ and P. Dutta. A new design for efficient dielectrophoretic separation of cells in a microdevice . Electrophoresis 2013, 34, 643–650
Schutte J, Freudigmann C, Benz K, Bottger J, Gebhardt R and Stelzle M 2010 A method for patterned in situ biofunctionalization in injection-molded microfluidic devices Lab Chip 10 2551–8
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Steven S. Saliterman
Detection Strategies…
Image courtesy of Olympus Gencoglu, A and Adrienne R. Minerick . Electrochemical detection techniques in micro- and nanofluidic devices. Microfluid Nanofluid (2014) 17:781–807
Image courtesy of Micronit
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Steven S. Saliterman
Organ-on-a-Chip…
PDMS membranes. Perfusion bioreactor and synthetic microfabricated scaffold.
a) Huh, D. et al. Reconstituting organ- level lung functions n a chip. Science 328, 1662–1668 (2010). b) Zhang, B. et al. Biodegradable scaffold with built- in vasculature for organ- on-a- chip engineering and direct surgical anastomosis. Nat. Mater. 15, 669–678 (2016).
Reproducing the Tissue Barrier Function
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Steven S. Saliterman
From Organ to Body-on-a-Chip…
Lung-on-a-Chip Body-on-a-Chip “Organ Coupling”
Zhang B, Korolj A, Lai BFL, Radisic M. Advances in organ-on-a-chip engineering. Nature Reviews Materials. 2018;3(8):257-278.
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Steven S. Saliterman
Clinical Laboratory Medicine
http://www.umass.edu/microbio/rasmol/padlan.htm Laposata M, Laboratory Medicine, Clinical Pathology in the Practice of Medicine, ASCP Press, Chicago (2002).
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Steven S. Saliterman
Microactuators and Drug Delivery
Sutradhar KB, Sumi CD. Implantable microchip: the futuristic controlled drug delivery system. Drug Deliv. 2016;23(1):1-11.
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Steven S. Saliterman
Genomics, Proteomics and µTAS
Esfandyarpour R, Esfandyarpour H, Harris JS, Davis RW. Simulation and fabrication of a new novel 3D injectable biosensor for high throughput genomics and proteomics in a lab-on-a-chip device. Nanotechnology. 2013;24(46):465301-465301.
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Steven S. Saliterman
Clinical Applications
Neurovent P-tel implantable piezoresistive ICP monitoring sensor. Telemetric reader is placed over intact skin and collects intracranial pressure readings. Image courtesy of Raumedic, Inc.
iSTAT cartridge and handheld system. Image courtesy of Abbot Laboratories.
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Steven S. Saliterman
Biocompatibility, FDA & ISO 10993
Barkam, S, et al. Fabricated micro-nano devices for in vivo and in vitro biomedical applications. WIREs Nanomed Nanobiotechnol 2013, 5:544–568
Image courtesy of Voskerician, G. Image courtesy of NAMSA
Barkam, S, et al. Fabricated micro-nano devices for in vivo and in vitro biomedical applications. WIREs Nanomed Nanobiotechnol 2013, 5:544–568
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Steven S. Saliterman
Packaging, Power, Data & RF Safety
Lab-on-a-chip / µTAS Interconnections. Biocompatibility with specimen. Reagent addition and waste removal. Date of expiration.
Other BioMEMS/MEMS devices Sensors and actuators. Point-of-care and human interface.
Implanted devices A device that is either partly or totally
introduced, surgically or medically, into the human body and is intended to remain there after the procedure.
Biocompatibility with the body. Power and wireless communication.
Microsurgical instruments.
Image courtesy of Valtronics
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Steven S. Saliterman
Energy Harvesting…
Lueke, J. and W.A. Moussa. MEMS based power generation techniques for implantable biosensing applications. Sensors 2011, 11, 1433-1460 Hannan, MA et al. Energy harvesting for implantable biomedical devices: issues and challenges. BioMedical Engineering OnLine 2014, 13:79
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Steven S. Saliterman
Team Projects
Purpose: To study further a particular bioMEMS concept or device that you are interested in.
Format: Team presentation of 4 students as a 20 minute Power Point® presentation at the end of the semester. Submitting a paper is not required, although you may wish to distribute a handout. A brief class discussion will follow each talk.
Description: Propose a new bioMEMS device, or expand upon a previously published device or useful methodology. Discuss the purpose of your concept, and if appropriate, the theory (what principles are at work), fabrication (materials and techniques), testing, limitations, and biocompatibility of your device.
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Steven S. Saliterman
Projects 2019…
3-ω Sensor for Property Measurement of Biomaterials A Novel, Portable Device to Count CD4+ T Cells using Flow Cytometry and a
Cellphone Diagnosis of Sepsis By Measurement of Spontaneous Neutrophil Motility Fetal Chromosomal Abnormalities Detection Chip Highly Stretchable Tactile Sensor Pressure Detection during Colonoscopy Miniaturized Cantilever Device for High-Throughput Analysis of Muscle Cell
Contractile Forces Monitoring Chronic Kidney Disease Through the Use of BioMEMs Non-Invasive, Continuous, Wearable Blood Glucose Monitor Optical Immunoassay for Snake bite Diagnosis Piezoelectric Cupular Micro Transducer Implant Shrink-based Device for Protein Purification Single-Neuron Gene Delivery via a PDMS-Based Multielectode Array ThyChyp
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Steven S. Saliterman
Summary
1) Nano- and Microfabrication of Silicon & Polymers. 2) Microfluidics - Design, Transport, and Electrokinetics. 3) Biosensors, Microsensors and Nanotechnology. 4) Lab, Organ and Body-on-a-Chip Systems. 5) Microactuators & Drug Delivery. 6) Clinical Laboratory Medicine & Micro Total Analysis Systems. 7) Genomics and Proteomics - Gene and Protein Chips. 8) Clinical Applications & Point-of-Service Devices. 9) Biocompatibility, FDA & ISO 10993. 10) Packaging, Power, Data & RF Safety.