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Course No : AEL –ENT-486

Course Title : Mass production of bio agents & bio-pesticides

Credits = (0+20=20)

Presented by….

BALRAM SOLUNKEbalram2424@gmail.com

Introduction To Bio Control

Laboratory

Location of BCL:- Name of department :-Agril. Entomology

Name of college :- K. K. Wagh college of agril. Nashik-03

Name of farm area:- Puriya park, Saraswati nagar Panchvati Nashik-03

Name of university :- M.P.K.V ,Rahuri

External view of bio control laboratory

Experiential learning Module ActivityCourse No. AEL-ENT-486Title : Mass production of bioagent and biopesticidesCredits=0+20Major Department: Agril. EntomologyAssociated subjects: Economics, Extension EducationNo. of students Registered: 14

Activities carried out by students: Rearing of host insect like Corcyra cephalonica, Mealy bug Rearing of Bioagents like Trichogramma, Cryptolaemus, Zygogrmma etc.Mass production of HaNPV and SlNPV Mass production of Metarhzium, Nomuraea, Verticelium, Beavaria,Trichoderma & Psudomonus etc.

No. of cells of Biocontrol lab :-

1) Sterilization room2) Media room3) Inoculation room4) Growth room 15) Growth room 26) Store room7) Mixing room18)Mixing room29) Insect rearing room110) Insect rearing room211)Bio agent room112)Bio agent room213)NPV room14)Washing room

STERILIZATION ROOM :

1) use of this room for sterilization through the Autoclave.

2) This room should be well ventilated by fitting exhaust fan.

3) In this room should be 2 autoclave, a horizontal & a vertical autoclave & Hot air oven.

For maximum flexibility, autoclave space is recommended on each floor, or at a minimum in a convenient location in each building, where microbiological research is performed. AUTOCLAVE

Media preparation room :

This room should get a sufficient & continuous supply of single & double-distillation unit ,pressure cooker , Exhust fan.

Inoculation room :

Inoculation room :

1) In this room the inoculation cabinet laminar air flow can be kept.

2) The floor of this room should be covered with linoleum or tiles to facilitate proper cleaning.

3) It is essential that the atmosphere in this room should be free from contaminants.

4) This room should preferably be air-conditioned because it ensures good air-circulation & helps in maintaining constant temp.

GROWTH ROOM :

GROWTH ROOM :1)In this room all inoculated bottles with spores

are kept.2)This room should be in sterile condition3)Air condition should be there to control

temperature.4)The following fungi are grow in this room :a)Verticillium lecaniib)Beauveria bassianac) Nomuraea rileyid)Metarrhizium anisopliaee)Trichodemma spp.

STORE ROOM :1) In this room store in Chemicals 2) Then Chemicals are Agar agar,

ethyl alcohol, peptone, streptomycine, talc powder etc.

3) The chemical rack should be arrange with A to Z alphabetical latters so its easy to find chemicals.

MIXING ROOM1) Grinding of different

fungus like trichoderma,verticilium,beauveria,metarhizium etc.

2) Mixing the mycelium culture with talcum powder

3) Drying of blended culture

4) Equipment: laboratory blander

two types of microscopeI. Steriozoom microscopeII. binocular microscope

INSECT REARING ROOM :1) In this room rearing of

helicoverpa larvae & spodoptera .

2) Rearing of moth of helicoverpa & spodoptera moth in glass chamber.

3) Rearing of mealy bug on pumpkin as a food for cryptolamous its use in off season when the mealy bug can not be available.

MICROVAVE OVEN :

Microwave oven use to heat the content of artificial diet.

NPV ROOM :1) This room is also called as virus

room.2) All viral processed taken in this

room.3) NPV made in this room.4) The Infected food i.e. .gram, A.diet given to the

helicoverpa larvae given in this room.

5) Equipment : centrifugal, laboratory blender, rack, exhaust, fan & furniture.

6) ventilation system provide in this room for exhaust of dirty smell.

WASHING ROOM :

1) The room should be provided with necessary service lines as water ,cleaning materials i.e. soap, sodium chloride .

2) In two side of the room a washing sink can be fitted.

3) The drainage & drying racks could be accommodated in this room.

THANK YOU