introduction - brechbühler ag · 2 0 200 mau 17420 0 2 4 6 min 1 2 0 200 mau 17421 0 2 2 1 4 6 8...

2

IntroductionPhenomenex is proud to introduce Lux™, our new line of

polysaccharide-based chiral stationary phases.

We know chiral separations can be challenging. With many

different product offerings in the industry, it may be difficult to

find the appropriate column to fit your needs.

Lux™ columns are engineered to provide a straightforward

approach to enantiomeric recognition and separation through

HPLC and SFC.

Lux™ columns are offered with two chiral stationary phases

(CSPs), both of which use coated derivatized cellulose as the

chiral selector. With new phases continuously in development,

you can rest assured that Lux™ columns will meet your chiral

separation demands of today as well as provide the latest in

chiral separation technologies for years to come.

3

Overview of Lux™ Chiral Stationary PhasesFeatures

Lux™ Cellulose-2

Lux™ Cellulose-1

Broad chiral recognition

For screening of new chiral compounds

Cellulose backbone

Increased loading capacity

Scalable 3 μm and 5 μm media

Stable in NP, Polar Organic, SFC and RP conditions

Novel selectivity

Similar selectivity to *CHIRALCEL® OD-H®

Cost-effective replacement to *CHIRALCEL® OD-H®

Table of ContentsChoosing the Right Chiral Stationary Phase ..............3

Chiral Recognition in Cellulose Phases ......................4

Inside Lux™ Chiral Stationary Phases .........................5

Lux™ Cellulose-2 ........................................................6

Lux™ Cellulose-1 ......................................................12

Ordering Information ................................................18

Trademarks Lux, Axia, SecurityGuard and Phenex are trademarks of Phenomenex, Inc. *CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd.

Disclaimer Comparative separations may not be representative of all applications. Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.

© 2008 Phenomenex, Inc. All rights reserved.

Phenomenex | Web: www.phenomenex.com

4

Cellulose Backbones

Over the past two decades, derivatized cellulose has

established itself as one of the most universal platforms

for separating racemic mixtures. It offers a variety of chiral

recognition mechanisms, including:

steric effects (attractive and repulsive)•

hydrogen bonding•

π - π interactions•

dipole interactions•

ionic interactions•

Broad Solvent System Compatibility

Derivatized cellulose is often compatible with a wide range of

solvent systems:

Normal Phase•

Polar Organic•

SFC (Supercritical Fluid Chromatography)•

Reversed Phase•

Chiral Recognition Mechanisms in Cellulose Phases


5

Enantioselective Polysaccharide Derivatives

Lux™ columns utilize polysaccharide derivatives that yield

broad-ranged enantiomeric selectivity.

Benefits include:

Unique halogenated substitution with • Lux™ Cellulose-2

Improved resolution with many separations using • Lux™ Cellulose-1 compared to *CHIRALCEL® OD-H®.

Specialized Coating Process

Lux™ media undergoes a specialized coating process onto

high quality silica. Significant benefits from this process

include:

High loading capacity•

Stable, reproducible phases•

Stability in a variety of solvents, including SFC conditions• †

Scalable Particle Sizes

Lux™ columns come in 3 μm and 5 μm particle sizes to

accommodate all your resolution and throughput needs.

pH Stability

Lux™ columns are pH stable from 2 – 8, providing options for

reversed phase chiral method development.

Complete Package Solution

No need to hassle with different columns or part numbers

if your chiral methods take you down different routes of

development. Lux™ columns are compatible with different

solvent strategies:

Normal Phase•

Polar Organic•

SFC (Supercritical Fluid Chromatography)•

Reversed Phase•

Inside Lux™ Chiral Stationary PhasesAlthough there may be various polysaccharide-based columns

on the market, it is important to note the following

about Lux™ chiral stationary phases:

†Consult the Lux column care and use notes for full details on solvent limitations, which can be found at www.phenomenex.com/info/luxnotes*CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd.


6

O

O

C

H

N

O

HH

C

C

C

C

C

CC

H

H

H

H

Cl

O

O

O

N

C

O

H

N

Cl

H

H

HH

C

C

H

HC

H

H

H

O

H

Cl

Cellulose-2

O

O

C

H

N

O

HH

C

C

C

C

C

CC

H

H

H

H

Cl

O

O

O

N

C

O

H

N

Cl

H

H

HH

C

C

H

HC

H

H

H

O

H

Cl

Phenyl rings contribute to π- π interactions.

Chlorine atoms have strong electronegativity, contributing to dipole interactions.

Cellulose backbones form linear chains, creating various grooves and cavities which provide steric and inclusion interactions.

Lux™ Cellulose-2 uses the chiral selector cellulose tris(3-chloro-4-methylphenylcarbamate)

Electronegative atoms, such as oxygen, nitrogen, and chlorine are responsible for hydrogen bonding.

7

O

O

C

H

N

O

HH

C

C

C

C

C

CC

H

H

H

H

Cl

O

O

O

N

C

O

H

N

Cl

H

H

HH

C

C

H

HC

H

H

H

O

H

Cl

O

O

C

H

N

O

HH

C

C

C

C

C

CC

H

H

H

H

Cl

O

O

O

N

C

O

H

N

Cl

H

H

HH

C

C

H

HC

H

H

H

O

H

Cl

Lux™ Cellulose-2Advanced chiral selector provides a unique range of enantioselectivity

Lux™ Cellulose-2 incorporates an advanced halogenated

derivative, leading to unique enantioselectivity compared to

previously commercialized cellulose phases.

The chiral selector is a cellulose

tris(3-chloro-4-methylphenylcarbamate)

It has been established that the introduction of either electron-

donating or electron-withdrawing groups, such as chlorine,

tends to improve the optical resolution abilities of chiral

stationary phases (CSPs) with phenylcarbamate derivatives.

In addition, substitution at the meta or para position on the

phenyl moiety is considered more beneficial than at the ortho

position to establish higher chiral recognition.1

The unique derivatization of Lux™ Cellulose-2 makes it an

ideal CSP to add to all chiral screening protocols and provides

excellent complementary selectivity to Lux™ Cellulose-1 in

many applications.

Studies show that for a set of 14 racemates of varying

properties, the 3-chloro-4-methylphenylcarbamate derivative

of cellulose was one of two derivatives, separating all 14

compounds with reasonable selectivity, with some better than

dimethylphenylcarbamate derivatives of cellulose.1

1. Bezhan Chankvetadze, Eiji Yashima, Yoshio Okamoto, J. Chromatography, 670 (1994) 39-49


8

Lux™ Cellulose-2 offers novel selectivity, improving chances of chiral resolution during multiple-column screening processes.

Lux™ Cellulose-2 vs. *ChIRaLCeL® OD-h®

0 2

21

4 6 min

0

200

400

600

800

mAU

17367

0 2

21

4 6 min

0

200

400

600

800

mAU

17368

17309

0 2

2

1

4 6 8 10 min

0

200

mAU

0 2

21

4 6 8 10 min

17310

0

200

mAU

0 2

2

1

4 6 min

0

200

400

600

mAU

17322

0 2

2

1

4 6 min

0

200

400

600

mAU

17323

Bupivacaine

Tolperisone

Miconazole

NNH

OH3C

CH 3

H 3C

N

N

O

Cl

Cl

Cl

Cl

*CHIRALCEL® OD-H®

a = 1.12

Lux™ Cellulose-2a = 1.18



*CHIRALCEL® OD-H®

a = 1.07

*CHIRALCEL® OD-H®

a = 1.08

Ap

p ID

173

09A

pp

ID 1

7322

Ap

p ID

173

67A

pp

ID 1

7310

Ap

p ID

173

23A

pp

ID 1

7368

Column: Lux™ 5 µm Cellulose-2Dimensions: 250 x 4.6 mm ID

Part No.: 00G-4457-E0Mobile Phase: 0.1 % Diethylamine in Hexane /

0.1 % Diethylamine in Isopropanol (90:10)Flow Rate: 1 mL/minDetection: UV @ 220 nm

Temperature: Ambient

Column: *CHIRALCEL® 5 µm OD-H®

Dimensions: 250 x 4.6 mm IDMobile Phase: 0.1 % Diethylamine in Hexane /




Part No.: 00G-4457-E0Mobile Phase: 0.1 % Diethylamine in Methanol /




Dimensions: 250 x 4.6 mm IDMobile Phase: 0.1 % Diethylamine in Methanol / 0.1 % Diethylamine

in Isopropanol (90:10)Flow Rate: 1 mL/minDetection: UV @ 220 nm










N

O

H 3CCH3

*CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd. Comparative separations may not be representative of all applications.


9

17387

0 105 15

2

1

20 min

0

200

400

600

800

1000

1200

1400

mAU

17388

0

200

400

600

800

1000

1200

1400

mAU

0 5 1510

2

1

20 min

17330

0 2

2

1

4 6 min

0

200

400

mAU

0 2

21

4 6 8

0

200

400

mAU

17331

17349

0 2

2

1

4 6 8 min

0

200

400

mAU

17350

0 2

2

1

4 6 8 min

0

200

400

mAU

Tramadol

N-(3,5-Dinitrobenzoyl)-a’-methylbenzylamine

Mephenytoin

N

HN

O

CH3

H 3C

O




*CHIRALCEL® OD-H®

a = 1.69

*CHIRALCEL® OD-H®

a = 1.19

*CHIRALCEL® OD-H®

a = 1.13A

pp

ID 1

7330

Ap

p ID

173

49A

pp

ID 1

7388

Ap

p ID

173

87A

pp

ID 1

7331

Ap

p ID

173

50






















Dimensions: 250 x 4.6 mm IDMobile Phase: 0.1 % Diethylamine in Hexane / 0.1 % Diethylamine

in Isopropanol (60:40)Flow Rate: 1 mL/minDetection: UV @ 220 nm


NCH 3

H

OH

H 3C

H 3CO

Additional applications are available upon request.

O–O

–ON+

N+

O

HN

O



10

0 2

21

4 6 min

0

200

400

600

800

1000

mAU

17396

0 2 4 6 min

12

0

200

mAU

17420

0 2 4 6 min

1 2

0

200

mAU

17421

0 2

2

1

4 6 8 min

0

2000

mAU

17461

0 2

21

4 6 8 min

0

2000

mAU

17462

Econazole

Terfenadine

Etozolin



*CHIRALCEL® OD-H®

a = 1.04

*CHIRALCEL® OD-H®

a = 1.39

*CHIRALCEL® OD-H®

a = 1.23

Ap

p ID

174

20A

pp

ID 1

7395

Ap

p ID

173

96A

pp

ID 1

7462

Ap

p ID

174

61A

pp

ID 1

7421






Dimensions: 250 x 4.6 mm IDMobile Phase: 0.1 % Diethylamine in Methanol /




Part No.: 00G-4457-E0Mobile Phase: 0.1 % Diethylamine in Acetonitrile /




Dimensions: 250 x 4.6 mm IDMobile Phase: 0.1 % Diethylamine in Acetonitrile /











N

N

Cl

ClO

Cl

N

O H

O H

C(CH3)3

Lux™ Cellulose-2 vs. *ChIRaLCeL® OD-h® (continued)

N

SN O

O

CH 3

O CH 3

0 2

2

1

4 6 min

0

200

400

600

800

1000

mAU

17395




11


0 2

21

4 min

0

200

mAU

17455

0 2

21

4 min

0

200

mAU

17456

0 2

2

1

4 6 min

0

200

mAU

17458

0 2

21

4 6 min

0

200

mAU

17459

0 2

2

1

4 6 8 10 min

0

200

400

600

mAU

17464

0 2

21

4 6 8 10 min

0

200

400

600

mAU

17465

Bifonazole

Mirtazapine

Nomifensine




*CHIRALCEL® OD-H®

a = 1.11

*CHIRALCEL® OD-H®

a = 1.24

*CHIRALCEL® OD-H®

a = 1.07

Ap

p ID

174

56A

pp

ID 1

7458

Ap

p ID

174

59A

pp

ID 1

7465

Ap

p ID

174

64A

pp

ID 1

7455

























NN

N

CH3

NCH 3

NH 2

N

N



12

H

HH

H

HC

C

H

HH

C

C

H

HH

H

O

O

C

H

N

O

HH

C

C

C

C

C

CC

H

H

H

H

O

O

O

N

C

O

H

N

HH

H

HH

C

C

H

O

H

H

HH

H

HC

C

H

HH

C

C

H

HH

H

O

O

C

H

N

O

HH

C

C

C

C

C

CC

H

H

H

H

O

O

O

N

C

O

H

N

HH

H

HH

C

C

H

O

H

Cellulose backbones form linear chains, creating various grooves and cavities which provide steric interactions.

Phenyl rings contribute to π- π interactions.

Electronegative atoms, such as oxygen and nitrogen, are responsible for hydrogen bonding.

Cellulose-1

Lux™ Cellulose-1 uses the chiral selector cellulose tris(3,5-dimethylphenylcarbamate)

13

H

HH

H

HC

C

H

HH

C

C

H

HH

H

O

O

C

H

N

O

HH

C

C

C

C

C

CC

H

H

H

H

O

O

O

N

C

O

H

N

HH

H

HH

C

C

H

O

H

H

HH

H

HC

C

H

HH

C

C

H

HH

H

O

O

C

H

N

O

HH

C

C

C

C

C

CC

H

H

H

H

O

O

O

N

C

O

H

N

HH

H

HH

C

C

H

O

H

Lux™ Cellulose-1The new choice for dimethylphenylcarbamate separations

Lux™ Cellulose-1 features a classical cellulose derivative used

industry-wide for many enantiomeric separations.

The derivative is cellulose

tris(3,5-dimethylphenylcarbamate)

This particular chiral selector has well established abilities

in resolving a wide range of racemates. Lux™ Cellulose-1

provides enhanced resolution for a variety of applications

compared to similar CSPs.

This derivative of cellulose has a well established history

of broad enantioselectivity. The substitution of the electron

donating methyl groups at the 3 and 5 position enhance

the activity of the phenyl group. This leads to enhanced

enantioselectivity for aromatic and conjugated compounds.

In addition, Lux™ Cellulose-1 undergoes a specialized

manufacturing and coating process, which, as shown in this

brochure, provides greater resolution for many applications

compared to *CHIRALCEL® OD-H® .

*CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd.


14

17314

0 2

2

1

4 6 min

0

200

400

1000

800

600

mAU

17316

0 2

2

1

4 6 min

0

200

400

1000

800

600

mAU

Lux™ Cellulose-1 offers exceptional separations for your existing polysaccharide methods.

Naringenin

Orphenadrine

Lux™ Cellulose-1 vs. *ChIRaLCeL® OD-h®


17327

2

2

1

4 6 min0

0

200

400

600

mAU

0 2

2

1

4 6 min

17328

0

200

400

600

mAU

Ap

p ID

173

14A

pp

ID 1

7316

Ap

p ID

173

27A

pp

ID 1

7328



*CHIRALCEL® OD-H®

a = 1.24

*CHIRALCEL® OD-H®

a = 1.94

OHO

O H O

O H

O

CH 3

NCH3

CH3


Part No.: 00G-4459-E0Mobile Phase: 0.1 % Formic acid in Hexane /

0.1 % Formic acid in Isopropanol (60:40)Flow Rate: 1 mL/minDetection: UV @ 220 nm



Dimensions: 250 x 4.6 mm IDMobile Phase: 0.1 % Formic acid in Hexane /

0.1 % Formic acid in Isopropanol (60:40)Flow Rate: 1 mL/minDetection: UV @ 220 nm











15

0 2

2

1

4 6 8 min

17319

0

200

400

mAU

17320

0 2

2

1

4 6 8 min

0

200

400

mAU

0

200

400

600

mAU

0 2

2

1

4 6 8 10 12 min

17341

0

200

400

600

mAU

0 2

2

1

4 6 8 10 12 min

17342

Bisoprolol

Mebeverine


Ap

p ID

173

19A

pp

ID 1

7342

Ap

p ID

173

41A

pp

ID 1

7320



*CHIRALCEL® OD-H®

a = 1.83

*CHIRALCEL® OD-H®

a = 1.24

H 3CO

H 3CO

O

ON

CH3

CH3

OCH3

O

OO CH3

CH3NH

H3C

O H

CH 3



















16

Indapamide

Dimethindene

Homatropine


0 2 4 6 min

0

200

400

600

mAU

17365

1

2

0 2

2

1

4 6 min

0

200

400

600

mAU

17366

0 2 4 6 min

0

200

400

mAU

17376

1

2

0 2 4 6 min

0

200

400

mAU

1

2

17377

0 1 2 3 4 5 min

1

2

0

200

400

600

800

mAU

17378

0 1 2 3 4 5 min

1

2

0

200

400

600

800

mAU

17379




*CHIRALCEL® OD-H®

a = 1.67

*CHIRALCEL® OD-H®

a = 2.07

*CHIRALCEL® OD-H®

a = 1.20

Ap

p ID

173

65A

pp

ID 1

7366

Ap

p ID

173

78A

pp

ID 1

7379

Ap

p ID

173

77A

pp

ID 1

7376

N

HN

CH 3

O

SNH 2

Cl

O O

N

O

O

O H

CH 3

H 3C

NCH 3

CH3

N






Dimensions: 250 x 4.6 mm IDMobile Phase: 0.1 % Diethylamine in Methanol / 0.1 %

Diethylamine in Isopropanol (90:10)Flow Rate: 1 mL/minDetection: UV @ 220 nm


















Lux™ Cellulose-1 vs. *ChIRaLCeL® OD-h® (continued)


17

Toliprolol

Mephenesine

Increase efficiencies by scaling down to 3 µm

Increase efficiencies of difficult separations of by scaling down

from 5 µm to 3 µm particles, available on both Lux™ Cellulose-1

and Lux™ Cellulose-2. 3 µm


0 1 2 3 4 min

0

200

400

mAU

17372

2

11

0 1 2 3 4 min

0

200

400

mAU

1

2

17373

min0 2 4 6 8 10 12 14

min0 2

21

21

4 6 8 10 12 14

mAU

0

100

200

300

400

mAU

0

100

200

300

400 Lux™ Cellulose-15 µma = 1.16

Lux™ Cellulose-13 µma = 1.17


*CHIRALCEL® OD-H®

a = 1.50A

pp

ID 1

7470

Ap

p ID

174

69A

pp

ID 1

7373

Ap

p ID

173

72

OHN CH3

O H

CH3

H 3C

















CH 3

O OH

OH



If you are not completely satisfied with Lux™ analytical columns, send in your comparative data within 45 days and KEEP THE COLUMN FOR FREE.

Lux™ Ordering Information

Extending Lifetime

Phenomenex recommends the use of SecurityGuard™ guard

cartridges to extend the lifetime of your column, especially with

samples extracted from complex matrixes. Ideally, samples must

be completely dissolved in the mobile phase or filtered through a

syringe filter of approximately 0.45 µm porosity.

18

Our promise of excellence doesn’t end with the delivery of our products.

Phenomenex pledges to provide on-going technical support and

customer service that matches the quality of our products. With offices

and a network of distributors worldwide, your needs will be taken care of

no matter where or when you call upon us.

Whether it’s for ordering, technical consulting, troubleshooting, or any

other array of needs, our team is knowledgeable and ready to assist you

each step of the way.

Go ahead and make the call – we’re waiting!


3 μm Analytical Columns (mm) SecurityGuard™ Cartridges (mm)150 x 2.0 100 x 4.6 150 x 4.6 250 x 4.6 4 x 2.0* 4 x 3.0*

Phases / 8pk / 8pk

Cellulose-1 00F-4458-B0 00D-4458-E0 00F-4458-E0 00G-4458-E0 AJ0-8402 AJ0-8403

Cellulose-2 00F-4456-B0 00D-4456-E0 00F-4456-E0 00G-4456-E0 AJ0-8398 AJ0-8366

for ID: 2.0–3.0 mm 3.2–8.0 mm

5 μm Analytical Columns (mm)SecurityGuard™ Cartridges (mm)

100 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0

Phases / 8pk

Cellulose-1 00D-4459-E0 00F-4459-E0 00G-4459-E0 AJ0-8403

Cellulose-2 00D-4457-E0 00F-4457-E0 00G-4457-E0 AJ0-8366

for ID: 3.2–8.0 mm

5 μm Axia™ Packed Preparative Columns (mm) SecurityGuard™ Cartridges (mm)150 x 21.2 250 x 21.2 250 x 30 250 x 50 15 x 21.2** 15 x 30.0+

Phases / ea / ea

Cellulose-1 00F-4459-P0-AX 00G-4459-P0-AX 00G-4459-U0-AX 00G-4459-V0-AX AJ0-8405 AJ0-8406

Cellulose-2 00F-4457-P0-AX 00G-4457-P0-AX 00G-4457-U0-AX 00G-4457-V0-AX AJ0-8400 AJ0-8401

for ID: 18–29 mm 30–49 mm*SecurityGuard Analytical Cartridges require holder, Part No. : KJ0-4282**PREP SecurityGuard Cartridges require holder, Part No. : AJ0-8223+PREP SecurityGuard Cartridges require holder, Part No. : AJ0-8277

Column Performance Check StandardPart No. Description Unit Price

AL0-8412 Chiral Test Mix No. 5 (Lux) ea

Tip: Try a Sample Pack!

The best way to determine if a specific Phenex membrane is suitable for your application.

Request yours today by phone or visit www.phenomenex.com/sample

If Phenex™ Syringe Filters do not perform as well or better than your current syringe filter product of similar membrane, diameter and pore size, send in your comparative data within 45 days and keep the Phenex products for FREE!

Phenex™ Syringe FiltersFor Sample and Solvent Filtration Prior to Chromatography!

Less system down time•

Consistent, reproducible results•

Increased column lifetime•

Phenex Offers:

Low absorption »

Maximum chemical »compatibility

Minimum extractables »

Excellent flow rate »

High total throughput »

Low hold-up volume »

Certified quality »

100 % integrity tested »

Easy pore identification »

Bi-directional use »

Above syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP).

1. 17 mm diameter.2. Glass fiber filters are 28 mm diameter and made of borosilicate. They will remove 90 % of all particles >1.2 µm. 3. Housing material is methacrylate butadiene styrene (MBS) polymerisate. Also known as Cryolite™.4. Cellulose acetate is surfactant-free.5. 26 mm diameter.6. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA.7. 28 mm diameter.8. Additional dimensions and membrane types are available. Please contact your local Phenomenex technical consultant or distributor

for availability or assistance.9. Larger quantity purchases at significant savings are available.

Cryolite is a trademark of CY/RO Industries. Phenex is a trademark of Phenomenex, Inc.

4 mm Diameterfor ≤ 2 mL sample volumes

15-17 mm Diameterfor 2 – 10 mL sample volumes

25-28 mm Diameterfor 10 – 100 mL sample volumes

Membrane Type/Size Part No. Unit Price Part No. Unit Price Part No. Unit Price

0.45 µm

Phenex-RC(Regenerated Cellulose)

AF0-3103-12 100/Pk AF0-2103-12 100/Pk AF0-8103-12 5 100/Pk

AF0-3103-52 500/Pk AF0-2103-52 500/Pk AF0-8103-52 5 500/Pk

Phenex-PES 3

(Polyethersulfone)

— — — AF2-5108-12 100/Pk AF0-8108-12 7 100/Pk

— — — — — — AF0-8108-52 7 500/Pk

Phenex-PTFE 6

(Polytetrafluoroethylene)

AF0-3102-12 100/Pk AF0-2102-12 100/Pk AF0-1102-12 100/Pk

AF0-3102-52 500/Pk AF0-2102-52 500/Pk AF0-1102-52 500/Pk

Phenex-NY(Nylon)

AF3-3107-12 100/Pk AF2-5107-12 100/Pk AF0-1107-12 100/Pk

AF3-3107-52 500/Pk AF2-5107-52 500/Pk AF0-1107-52 500/Pk

Phenex-GF/CA 2,3,4

Glass Fiber/Cellulose Acetate

An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. Excellent for filtration of tissue culture media, general biological sample filtration and clarification.

AF0-8B09-12 7 100/Pk

AF0-8B09-52 7 500/Pk

0.20 µm

Phenex-RC(Regenerated Cellulose)

AF0-3203-12 100/Pk AF0-2203-12 100/Pk AF0-8203-12 5 100/Pk

AF0-3203-52 500/Pk AF0-2203-52 500/Pk AF0-8203-52 5 500/Pk

Phenex-PES 3

(Polyethersulfone)— — — — — — AF0-8208-12 7 100/Pk

— — — — — — AF0-8208-52 7 500/Pk

Phenex-PTFE 6

(Polytetrafluoroethylene)

AF0-3202-12 100/Pk AF0-2202-12 100/Pk AF0-1202-12 100/Pk

AF0-3202-52 500/Pk AF0-2202-52 500/Pk AF0-1202-52 500/Pk

Phenex-NY(Nylon)

AF3-3207-12 100/Pk AF2-5207-12 100/Pk AF0-1207-12 100/Pk

AF3-3207-52 500/Pk AF2-5207-52 500/Pk AF0-1207-52 500/Pk

Phenex-GF/CA 2,3,4

(Glass Fiber/Cellulose Acetate)

An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. Excellent for filtration of tissue culture media, general biological sample filtration and clarification.

AF0-8A09-12 7 100/Pk

AF0-8A09-52 7 500/Pk1.20 µm

Phenex-GF 2,3

(Glass Fiber)An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. Excellent for filtration of tissue culture media, general biological sample filtration and clarification.

AF0-8505-12 7 100/Pk

AF0-8505-52 7 500/Pk

LuxembourgMaarssenbroeksedijk 13D3542 DL UtrechtNetherlands

+31 (0)30-2418700+31 (0)[email protected]

NetherlandsMaarssenbroeksedijk 13D3542 DL UtrechtNetherlands

[email protected]

mail:

tel.:fax:

email:

mail:

tel.:fax:

email:

BelgiumMaarssenbroeksedijk 13D3542 DL UtrechtNetherlands

+31 (0)30-2418700+31 (0)[email protected]

Canada411 Madrid Ave.Torrance, CA90501-1430USA

(800) 543-3681(310) [email protected]

DenmarkGydevang 39-413450 AllerødDenmark

4824 80484810 [email protected]

AustraliaPO Box 4084Lane Cove, NSW 2066Australia

[email protected]

AustriaZeppelinstr. 563741 AschaffenburgGermany

[email protected]

FranceParc des Grillons, Bat.360 route de Sartrouville78232 Le Pecq CedexFrance

01 30 09 21 1001 30 09 21 [email protected]

GermanyZeppelinstr. 563741 AschaffenburgGermany

[email protected]

IrelandQueens Avenue,Hurds�eld Ind. Est.,Maccles�eld, Cheshire SK10 2BN, UK

01 247 5405+44 [email protected]

mail:

tel.:fax:

email:

ItalyVia M. Serenari, 15/D40013 Castel Maggiore (BO)Italy

051 6327511051 [email protected]

New ZealandP O Box 31-601Milford 0741North Shore CityNew Zealand

[email protected]

Puerto Rico273 Sierra Morena,Suite #104San Juan,Puerto Rico 00926

(800) 541-HPLC(310) [email protected]

United KingdomQueens Avenue,Hurds�eld Ind. Est.,Maccles�eld, Cheshire SK10 2BN, UK

[email protected]

USA411 Madrid Ave.Torrance, CA90501-1430USA

(310) 212-0555(310) [email protected]

www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department by telephone, fax or email: [email protected].

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