introducing the easy-nlc™ split-less, nano-flow hplc system, for proteomics from

Introducing the EASY-nLC™

Split-less, nano-flow HPLC system, for proteomics from

Copyright © Proxeon Biosystems A/S2

Overview

The EASY-nLC™ is:

• Complete• Configured• Compact• EASY to

– install– learn– operate

• Performance


Complete

• 1 cooled autosampler– 96 or 384 well plates– Single or double height– Wash & Waste bottles

• switching valves

• nano-flow sensor

• nano-litre pumps

• Built-in PC & software


EASY2-nLC™ (with flow control)2-column

Waste

Solvent A

Solvent B

AFC™

AdvancedFlowControl system


EASY2-nLC™ (with flow control)1-column

Solvent A

Solvent B

AFC™

AdvancedFlowControl system


EASY2-nLC™ (with flow control)

Waste

Waste

2-column 1-column


Compact

• Small footprint – 35cm wide (14”)– 38cm deep (15”)– 45cm high (18”)

• Touch-screen controlled– built-in computer– no keyboard or mouse

required

• Light– 35 kg, all inclusive


Easy to install• Only a few cables required

– contact closure– [network is optional]– Power

• Software is – integrated, – pre-installed and – up-to-date

• Solvent purging, air removal and column flushing is fully automated

• Less than 4 hours from power up to first analytical data


Easy to learn

• Logical software structure• Practice oriented work flow

• Results oriented parameters– E.g. volume & flow rates,

not hardware control• Intuitive graphical

user interface


Easy to operate (1)

• What You See Is What You Get– Simple design– Logical layout– Clear separation

in compartments– Modular

components

• Easy access to all parts of instrument


Easy to operate (2)

• Automated maintenance scripts

• Intelligent trouble shooting software

• Web interface for software upgrades


Easy to operate (3)

• Split-less, nano-flow means:• Less solvent use• Less waste

• Robust design & Intelligent software control means:

• Less support • Less maintenance • More uptime

M T W T F S S

1 2 3 4 5 6

7 8 9 10 11 12 13

14 15 16 17 18 19 20

21 22 23 24 25 26 27

28 29 30

M T W T F S S

1 2 3 4 5 6

7 8 9 10 11 12 13

14 15 16 17 18 19 20

21 22 23 24 25 26 27

28 29 30


Example (1)• Mass spectrometer

– Finnigan LCQ Classic• Pre-column

– 2cm, 75µm ID, Reprosil-Pur C18-AQ. 5µm

• Analytical column– 10cm, 75µm ID, Reprosil-Pur

C18-AQ. 3µm• Solvents

– A: 0.005% HFBA, 0.4% acetic acid, water

– B: 0.005% HFBA, 0.4% acetic acid, 90% acetonitril, water

• Amount injected– 100 fmol tryptic digested BSA

• HPLC gradient– 300 nl/min, 10-100 %B in 25

minutes

6 out of 36 consecutive runs


Example (2)

• Carry-over– < 0.003%

• Sample pickup– From 0.2µl to full loop in

0.01µl steps– Loop size adjustable– RSD < 0.5%

• Peak widths (FWHM)– < 10-15s at 50%

• Reproducibility– RT RSD < 0.7%


Summary

The EASY-nLC™ is:• A fully integrated and

complete instrument• With true nano-flow and

on-line monitoring • Designed from the

ground up to be easy and intuitive to operate

• A nano-LC system for the 21st century

introducing the easy-nlc™ split-less, nano-flow hplc system, for proteomics from

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