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Leica Application Suite Integrated Software Solutions

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Leica Application SuiteIntegrated Software Solutions

Now scientists can spend 100% of their time

You’re In Focus!

Totally Integrated SolutionLeica Application Suite (LAS) integrates Leica automated microscopes, macroscopes, digital cameras and softwareinto one common environment to provide an easy to use and consistent imaging solution with unrivalled performance.The versatility of the Leica Application Suite means that it is suitable for a diverse range of life science and industri-al applications such as materials quality control, pathology, pharmaceutical testing and many other laboratory tasks.LAS accelerates the visualisation, enhancement, measurement and documentation of digital images through the rich-ness of its image processing functions. This powerful software solution can control all functions of the Leica DMrange of upright and inverted Compound Microscopes and motorised Stereo-microscopes and Macroscopes. By pro-viding all the necessary tools for the installed applications to communicate with each other as well as with peripheraldevices connected to the computer, LAS offers an intuitive solution that simplifies routine and research analysis.

Features at a glance:• A unique user interface designed for ultimate operator convenience and for all imaging software applications.• Increases productivity by integrating microscopes, digital cameras and application software to co-ordinate

imaging tasks with intelligent control.• Automates digital-microphotography environment through the computerised features of the Leica microscopes.• A single application for acquiring, storing, annotating and displaying high quality images with a thumbnail gallery.• High modularity starting from routine applications to cutting edge research procedures.• LAS is based on Windows PCs and provides a cost-effective and uniform environment, compatible across the

Leica range of microscopes and Digital FireWire cameras.

Demanding Microscopy!The common core functionality of Leica Application Suite creates an intelligent and automated micro-imaging envi-ronment and is supplied with each Leica microscope and digital camera as part of an integrated system solution. Thisprovides the basic software for configuration and control as well as for the acquisition, analysis and processing of highquality digital images. The core package includes the following features:

Features at a glance:• Microscope and digital camera configuration and control in a fully integrated manner.• Basic annotation tools to allow image data and measurement data to be added in order to indicate relevant

details.• Auto and manual exposure adjustments to allow fully optimised imaging conditions.• A thumbnail gallery of acquired images which can be reviewed quickly and easily.• Calibrations are calculated, based on data read from Leica microscopes and cameras and a scale bar indicates

image size.

3concentrating on their work and not on their tools. Rouven Heeb, Design Engineer Leica SMS

LAS offers you a higher precision image system for Christian Wild, Development Engineer Leica SMS

further analysis and quantification.

The professional design of LAS offers a unique user interface that uses a workflow organisation in order to createthe ultimate working environment based on user convenience.

SetupThe setup task bar provides a straightforward method of configuring the Leica microscope and camera to be usedduring image acquisition. All configurations such as establishing objective types and filter descriptions can be read-ily saved and recalled to ensure that imaging conditions are correctly reproduced.

AcquireLAS enables the easy acquisition of images, which can be subsequently added to a Gallery and stored in a definedfolder. With LAS, all camera controls can be set to individual requirements from exposure, gain and gamma, to his-togram black and white levels. Images can be acquired in a variety of sizes, colour depths and file formats to provideeven more flexibility. By setting the sharp image option and shading reference from the processing toolbar, imagescan be acquired at the highest detail necessary so that further processing requirements are minimal. LeicaApplication Suite also allows a focusing region to be defined on a live image so that areas of significance can be easilyidentified and focussed more rapidly. All parameters and configurations can be saved and recalled at a later date. * For more advanced acquisition options, additional modules can be added such as LAS MultiFocus, LAS Montage,LAS Image Overlay, LAS MultiStep and LAS MultiTime Timelapse and Movie Recording.

BrowseThe Browse function provides access to all associated information for each stored image such as the time of acqui-sition, the bit depth and the calibration. The directory browser means that images can be located quickly and easilythrough a simple navigation system whereby folders can be effortlessly located. The integrated gallery stores eachimage as a thumbnail to speed up the process of locating and retrieving image files. Furthermore, images can becopied and pasted to other applications, or zoomed and panned for a more detailed inspection. * To further improvethe browsing capabilities and search functions of LAS, the optional Image Organizer is available.

ProcessBy using the Process workflow tab, images can be further enhanced and refined. From brightness and saturation lev-els to contrast and gamma, each image can be adjusted to your requirements. Additionally, the basic annotation toolsmean that a file name, the time of acquisition and a brief description can be included to provide concise information.Even scale bars and lines can be added and individually customized. Annotations can either be saved with the imageof merged with it so that the data is still visible when exported. * To further enhance this functionality, the LASExtended Annotation module can be added to your system

These underlying capabilities can be enhanced with a range of advanced modules and applications to form a power-ful microscopy imaging environment. Each LAS module provides the flexibility to tailor a system solution to fulfil individual needs with upgrade options available for future requirements

Attention to Detail!

Imaging Modules To Suite Your Needs

The LAS Multifocus module is designed to easily create extended depth of field images from a series of partiallyfocused images by providing fully integrated control of microscopes with motorized focus. Exposure, gain and shad-ing can be individually set for user convenience.

The LAS Montage module is a powerful solution for creating and visualising extended focus images. The extensiverange of methods in conjunction with the preview capability makes LAS Montage a premier solution for extendedfocus image creation.

The LAS Image Overlay module is designed for multi-channel fluorescent imaging, performing tasks in a simple andautomatic manner. From visualization through to enhancements and the documentation of images, LAS ImageOverlay provides a highly professional application.

The LAS MultiTime module is a highly effective solution for the automatic acquisition of images at intervals from seconds to minutes. With its movie recording functionality, the LAS MultiTime module provides a simple method of acquiring a compressed image stream directly to the hard drive.

LAS MultiStep is a highly intelligent module that when used with a Leica microscope, motorized scanning stage anddigital camera, automatically acquires images at XY positions defined by a rectangular pattern. Mosaic images canbe created and manipulated and reduced in size in to more manageable dimensions.

The LAS Image Organizer application saves and retrieves data effortlessly in freely defined categories. Intelligentsearch functions simplify the process of finding and retrieving information. With its integrated store and recall function the microscope and camera settings can be easily restored.

The Store & Recall module allows you to benefit from the full digital integration of microscope and camera. Yourresults will be completely reproducible including e.g. light settings, contrasting method, magnification, exposuretime and many more.

You and other microscope users can create individual microscope profiles which are optimized for your samplesand applications. A user profilee not only covers all microscope accessories, including objectives andfilter cubes,but also accommodates your special personal settings.

MULTIFOCUS

MONTAGE AND 3D VIEWER

IMAGE OVERLAY

MULTITIME – TIMELAPSE AND MOVIE

MULTISTEP

IMAGE ORGANIZER

STORE & RECALL

MULTIUSER PACKAGE

© Leica Microsystems (Switzerland) Ltd., CH-9435 Heerbrugg, (Switzerland), www.leica-microsys

tems.com

If there is no CD-ROM inside please contact your local dealer orgo to www.leica-microsystems.com/LAS to download the files.

An Integrated MicroscopySolution

LAS Extended Annotation module that allows text and graphical elements to be added directly to an image includinglines, arrows, shapes images, times and text. All annotations can be customized to individual requirements.

The Leica Application Suite Interactive Measurement Module is designed to perform sophisticated measurements on areas of interest. Furthermore, objects can be counted individually and assigned to a labeled class.

Leica Steel Expert is a highly specialised software programme for automatic steel inclusion rating. This module is apowerful software solution that brings fast, reproducible and accurate results to the process of automated inclusionrating for stainless steel alloys.

The LAS Reticule application provides electronic means of viewing live images and overlaying reticule styles to provide visual feedback as to the approximate scale of the field of view. Object size comparisons and point countingmeasurements quickly and easily.

The Digital Autofocus module provides a precise focus position for your sample. You always get excellent resultssince the performance with respect tospeed and accuracy is optimized for each objective. Besides being highly convenient, the module is also cost effective because no additional gadgets are needed.

EXTENDED ANNOTATION

INTERACTIVE MEASUREMENT

STEEL EXPERT

RETICULE

DIGITAL AUTOFOCUS

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In accordance with the ISO 9001 certificate, Leica Microsystems (Switzerland) Ltd, Business UnitStereo & Macroscope Systems has at its disposal a management system that meets the require-ments of the international standard for quality management. In addition, production meets the requirements of the international standard ISO 14001 for environmental management.

Leica Microsystems – the brand for outstanding productsLeica Microsystems’ mission is to be the world’s first-choice provider of innovativesolutions to our customers’ needs for vision, measurement and analysis of micro-structures.

Leica, the leading brand for microscopes and scientific instruments, developed fromfive brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and CambridgeInstruments. Yet Leica symbolizes innovation as well as tradition.

Leica Microsystems – an international companywith a strong network of customer servicesAustralia: Gladesville Tel. +61 2 9879 9700 Fax +61 2 9817 8358Austria: Vienna Tel. +43 1 486 80 50 0 Fax +43 1 486 80 50 30Canada: Richmond Hill/Ontario Tel. +1 905 762 2000 Fax +1 905 762 8937Denmark: Herlev Tel. +45 4454 0101 Fax +45 4454 0111France: Rueil-Malmaison Tel. +33 1 47 32 85 85 Fax +33 1 47 32 85 86Germany: Bensheim Tel. +49 6251 136 0 Fax +49 6251 136 155Italy: Milan Tel. +39 0257 486.1 Fax +39 0257 40 3475Japan: Tokyo Tel. + 81 3 5421 2800 Fax +81 3 5421 2896Korea: Seoul Tel. +82 2 514 65 43 Fax +82 2 514 65 48Netherlands: Rijswijk Tel. +31 70 4132 100 Fax +31 70 4132 109People’s Rep. of China: Hong Kong Tel. +852 2564 6699 Fax +852 2564 4163Portugal: Lisbon Tel. +351 21 388 9112 Fax +351 21 385 4668Singapore Tel. +65 6779 7823 Fax +65 6773 0628Spain: Barcelona Tel. +34 93 494 95 30 Fax +34 93 494 95 32Sweden: Sollentuna Tel. +46 8 625 45 45 Fax +46 8 625 45 10Switzerland: Glattbrugg Tel. +41 44 809 34 34 Fax +41 44 809 34 44United Kingdom: Milton Keynes Tel. +44 1908 246 246 Fax +44 1908 609 992USA: Bannockburn/lllinois Tel. +1 847 405 0123 Fax +1 847 405 0164

and representatives of Leica Microsystemsin more than 100 countries.

The companies of the Leica Micro-systems Group operate internationally in three business segments, where werank with the market leaders.

• Microscopy SystemsOur expertise in microscopy is the basisfor all our solutions for visualization,measurement and analysis of micro-structures in life sciences and industry.With confocal laser technology and image analysis systems, we providethree-dimensional viewing facilities andoffer new solutions for cytogenetics,pathology and materials sciences.

• Specimen PreparationWe provide comprehensive systems and services for clinical histo- and cytopathology applications, biomedicalresearch and industrial quality assur-ance. Our product range includes instruments, systems and consumablesfor tissue infiltration and embedding, microtomes and cryostats as well as automated stainers and coverslippers.

• Medical EquipmentInnovative technologies in our surgicalmicroscopes offer new therapeutic approaches in microsurgery.