Integrate Your Biology with Agilent

Agilent Solutions for -Omics Challenges

March 2, 2015

Agilent Omics

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Agilent Solutions for -Omics Challenges

The complexity of biology presents enormous challenges to understanding even simple systems. The complexity of biology presents enormous challenges to understanding even simple systems.

Agilent is a trusted leader in developing:

• Instrumentation

• Consumables

• Analytical methods

• Software solutions

…needed to integrate multi-omics data.

March 2, 2015

Agilent Omics

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Metabolomics Analytical Challenge

Metabolomics is the comparative analysis of endogenous metabolites found in biological samples

Molecular weight 50 to 1600 Da

Number of primary metabolites ~ 2500

Number of secondary metabolites > 100,000

Concentrations span a wide dynamic range

Diverse chemical structures

• No single analytical method exists

Quantitate – absolute or relative

Metabolite identification of unknowns

March 2, 2015

Agilent Omics

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QQQ 6400 Series

Q-TOF 6500 series

TOF 6200 series

1290 Infinity II UHPLC

Hi-DEF Q-TOF 6500 series

7200B GC/Q-TOF

7000C GC/QQQ

5977A GC/MS

7100 CE 1260 SFC

Agilent Instrumentation For Metabolomics

Most Extensive Software Portfolio for Metabolomics Data Analysis

March 2, 2015

Agilent Omics

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Find features across many complex data files

Align extraction results from many files

Analyze extracted data for statistically different features

Annotate and identify interesting features

Search and map annotated metabolites to find interesting pathways

Visualize results on pathways

March 2, 2015

Agilent Omics

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Proteomics Analytical Challenge

Proteomics is the analysis of proteins in a biological system Approximately 20,000 proteins in humans

This equals approximately 1,000,000 tryptic peptides!

Analysis is typically done on enzymatically (trypsin) digested samples because • Peptides are easier to separate

• Peptides respond better by LC/MS than proteins

• MS/MS of peptides can be predicted

• Poor protein extraction and digestion lead to irreproducible results – bad data!

Concentrations span a wide dynamic range (about 1011 in plasma)

Quantitate – absolute or relative

Protein identification by matching peptide MS/MS to theoretical spectra

March 2, 2015

Agilent Omics

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Agilent Instrumentation For Proteomics

QQQ 6400 Series

Q-TOF 6500 series

HPLC-Chip

G1992A Nanospray source

Agilent Jet Stream source

1290 Infinity UHPLC

Agilent AssayMAP

Bravo

Most Extensive Software Portfolio for Proteomics Data Analysis

March 2, 2015

Agilent Omics

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Identify proteins from discovery data-dependent analysis

and/or

Target peptides for proteins of interest

Analyze data for statistically different features

Search and map annotated proteins to find interesting pathways

Visualize results on pathways

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Mass Profiler Professional

Differential Analysis

Protein Identification

Spectrum Mill

Proteomics

Agilent Microarrays Agilent SureSelect and HaloPlex Target

Enrichment

Agilent SureFISH

Agilent Genomics Products

• CGH Arrays • Gene Expression Arrays • miRNA Arrays

• Exomes and Targeted Panels • Custom Target Enrichment • RNA-Seq and Methyl-Seq

• Catalog of hundreds of probes • Custom probes

For Research Use Only. Not for use in diagnostic procedures

Design Prepare Samples

Process Samples

Data Analysis

Data Interpretation

Genomics Research Solutions

For Research Use Only. Not for use in diagnostic procedures

AriaMx Real-Time PCR System

Total Confidence qPCR for Today and Tomorrow.

March 2, 2015

Confidentiality Label

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FAST SCANNING, FAST CHEMISTRIES: • Fastest Scan Times – 3 seconds or less for all channels. • Fast Chemistries – including Brilliant III Ultra Fast Reagents • Factory Calibrated – for an even faster start.

QPCR FOR TODAY AND TOMORROW: • Modular, Inter-changeable Optical Modules • Multiple chemistries and open platform • Build in HRM Capabilities and Software for even more applications

SUPERIOR DATA & DATA ANALYSIS • Complete Software package including HRM analysis • Tight Thermal Control: Maintains within + 0.2 C or less of target temp • LEDs capture all channels with every scan

Synthetic Biology / Real Results

March 2, 2015

Agilent Omics

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Market Leading Mutagenesis + Cloning & Protein Expression

Key Investments in Products for Synthetic Biology:

- Protein Engineering Tools

- NEW! QuikChange HT Protein Engineering Kit

- Genome Engineering Tools

- New! SureGuide CRISPR/Cas Complete Nuclease Kit

- Next Generation Cloning Tools

Protein Engineering

Next Generation

Cloning Genome

Engineering

Oligo Library

Synthesis

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Agilent’s Platform for Integrated Biology

LC/MS GC/MS

Microarrays

MassHunter Qual/Quant ChemStation AMDIS

Feature Extraction

Alignment to Reference Genome NGS

For Research Use Only. Not For Use in Diagnostic Procedures.

GeneSpring Platform

Pathway Architect

Integrated Biology Software GeneSpring Suite

March 2, 2015

Agilent Omics

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GX mRNA Expression Alternative Splicing microRNA Expression Genome-wide Association Copy Number Variation Strand NGS DNA-Seq, RNA-Seq ChIP-Seq, Methyl-Seq, small RNA-Seq & SureSelect QC Mass Profiler Professional Proteomics Metabolomics Pathway Architect Multi-Omic Pathway Analysis Canonical and NL Pathways

Agilent-BridgeDB: Resolving the Mapping Problem Between Databases

March 2, 2015

Agilent Omics

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Translates identification names to pathway database names

Automatic – does not require user intervention

Metabolites Identifiers • KEGG, MetaCyc, PubChem. LMP,

HMDB, ChEBI, and CAS

Proteins Identifiers: • Swiss-Prot, UniProt, and

UniProt/TrEMBL

Genes Identifiers: • Entrez Gene, GenBank, Ensembl, EC

Number, RefSeq, UniGene, HUGO, HGNC, and EMBL

March 2, 2015

Agilent Omics

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Integrate Your Biology With Workflows From Agilent Comprehensive -omics workflows for metabolomics, proteomics, and genomics • Instrumentation and reagents • Acquisition and data analysis software

GeneSpring Suite of software for integrated biology • Pathway Architect supports pathway-directed experimentation

- Multi-omics support - Data visualization directly on pathway

• Correlation analysis for discovery of new biology/relationships - Valuable when pathways are unavailable or not relevant - Connects biology to meta data