instruction manual microtome m530 rotary microtome semi
TRANSCRIPT
Instruction manual Microtome M530
Rotary Microtome semi-automatic
Manufacturer MEDITE GmbH
and publisher: Wollenweberstraße 12
31303 Burgdorf
Germany
Read manual closely before putting device into
operation • Keep manual accessible with device •
Contact customer service in case of questions •
Specifications are subject to change without prior
notice.
Rev.: 2 – 06/2017
Declaration of Conformity
Type of instrument: Rotary Microtome semi-automatic
Model: M530
Serial No.:
We do hereby certify that the above mentioned product meets the requirements set forth in the CE Directives indicated below including all changes and addendums to date thereto. The above mentioned product has been controlled and meets the following directives and standards:
• Directive 2006/42/EC Machine
• Directive2004/108/EC EMC
• Directive 2006/95/EC Low Voltage
Burgdorf, 15.06.2015 M. Ott – Managing Director
Content
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
Content
1. Safety Instructions ............................................................................................. 1
1.1. About this Manual ................................................................................................... 1
1.2. Used Symbols and their meanings .......................................................................... 1
1.3. Hazard Sources ...................................................................................................... 1
1.4. Personal protective equipment ................................................................................ 2
1.5. Transport and stocking ............................................................................................ 2
1.6. Environmental conditions ........................................................................................ 2
1.7. Safety measures on installation location .................................................................. 3
1.8. Protectiom measures of the device ......................................................................... 3
1.9. Intended Use ........................................................................................................... 3
1.10. Approved Operators ............................................................................................ 3
2. Information about Microtome M530 .................................................................. 4
2.1. Shipment ................................................................................................................. 4
2.2. Technical Data ........................................................................................................ 4
2.3. Transport ................................................................................................................. 4
2.4. Overview components of the Microtome M530 ........................................................ 6
2.4.1. Front view ........................................................................................................ 6
2.4.2. Back Site .......................................................................................................... 7
2.4.3. Film Keyboard .................................................................................................. 7
2.4.4. Knife carrier ...................................................................................................... 8
2.4.5. Object clamp .................................................................................................... 8
3. Startup the Microtome M530 ............................................................................. 9
3.1. Preface .................................................................................................................... 9
3.2. Mounting ................................................................................................................. 9
3.3. Dismounting ............................................................................................................ 9
4. Operation .......................................................................................................... 10
4.1. Power On ...............................................................................................................10
4.2. Prepare the specimen ............................................................................................10
4.2.1. Object orientation ............................................................................................10
4.2.2. Insert the specimen .........................................................................................11
4.2.3. Remove the Cassette clamp ...........................................................................11
4.3. Prepare knife carrier ...............................................................................................12
4.3.1. Inserting the microtome blade .........................................................................12
4.3.2. Adjustment of the knife carrier .........................................................................13
Content
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
4.3.3. Adjustment of the blade holder ........................................................................13
4.3.4. Adjusting The Knife Carrier Angles ..................................................................14
4.3.5. Change the microtome blade ..........................................................................14
4.4. Use the handwheel brake .......................................................................................15
4.5. Start the cutting process .........................................................................................16
4.5.1. Trimming the specimen ...................................................................................16
4.5.2. Sectioning the specimen .................................................................................17
4.5.3. Automatic Retraction .......................................................................................17
4.5.4. Position Memory .............................................................................................17
4.6. Troubleshooting .....................................................................................................18
4.6.1. Change the Fuse .............................................................................................18
5. Cleaning, Maintenance and Recycling ........................................................... 19
5.1. Cleaning Instructions ..............................................................................................19
5.2. Timing of Cleaning And Maintenance .....................................................................19
5.3. Abandonment / Recycling.......................................................................................20
6. Notice ................................................................................................................ 21
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 1 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
1. Safety Instructions
1.1. About this Manual
This manual will help you to handling the device. Please read the manual completely and follow the advice. Acquaint yourself to the control elements and their functionality by studying the figures. This way, an adequate use of the device, is ensured. If you have problems, feel free to contact the competent MEDITE contact person.
1.2. Used Symbols and their meanings
1.3. Hazard Sources
Working with the machine contents crushing hazard from the hand wheel and the specimen clamp.
Crushing hazard: Working on moveable components of the machine should be avoided. These warnings are marked with a yellow triangle and a crushing hand inside.
Warning: This symbol warns you of risks for machine, material or environment. Warnings are marked with a yellow warning triangle.
Working with this machine contents cutting hazards from the knife carrier
and specimen clamp (moving).
Cutting hazard: Working with this machine contents cutting hazards for
fingers and hand. These warning are marked with a yellow triangle and a
cutting hand inside.
Notice: Notices, meaning important information for the user, that are not classified as danger or warning, are marked with an information symbol.
Danger warning: This symbol warns you of risks for the life or health of persons. Danger warnings are marked by a red framed triangle.
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 2 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
1.4. Personal protective equipment
When working with the Microtome M530, it is necessary, according to you security policies applicable in any case, to wear protective clothings to avoid direct contact with the biologic samples. The same applies, for example, when cleaning the unit.
1.5. Transport and stocking
Observe the following notes for transport and stocking the Microtome M530.
1.6. Environmental conditions
The permissible ambient conditions for operation of the device are listed below. Operation at other ambient conditions is not permitted.
• Operation is only allowed in enclosed rooms,
• Ambient temperature from +15°C to +40°C (without strong fluctuations),
• Only set it on a vibration-free, the weight of the device corresponding laboratory bench,
• Relative air humidity max 80% non-condensing,
• The unit must not be exposed to direct sunlight,
• Maximum height of 2000 m (according to EN 61010-1:2001),
• Mains voltage fluctuation is within + / - 10 % of rated voltage.
Always use the carrying device for lifting and carrying the Microtome M530 make sure to avoid a correct attitude about material things and especially physical damage when lifting!
The Microtome M530 is supplied in a safety transport box. Transporting them always upright and avoid shocks and extreme temperature fluctuations! For the storage of the device an ambient temperature of +5 ° C to +50 ° C is permissible.
Always wear protective clothing (e.g. lab coat, gloves,) to prevent accidental contact with biological samples.
„Top“: The packaged unit must always be transported and stored so that the arrows point upwards at any time.
"Protect from wetness": The packaged unit must be protected from excessive humidity and must be stored and transported in covered.
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 3 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
1.7. Safety measures on installation location
The Microtome M530 must be installed on a stable, level and horizontally oriented base to ensure the safe and reliable operation.
1.8. Protectiom measures of the device
1.9. Intended Use
The Microtome is a highly efficient multi-purpose microtome which can be used for any tasks
in paraffin, hard- and thin-sectioning techniques in biology, medicine, industry and research.
A use in food industries and similar facilities is strictly prohibited. Arbitrary rebuilding or
changing of this laboratory instrument is strictly prohibited due to safety reasons. Use only
original MEDITE spare parts in case of replacement issues.
Operation, maintenance or service conditions mentioned in this manual have to be strictly observed.
1.10. Approved Operators
Only persons who have been thoroughly trained by the operator and commissioned can handle the Microtome M530. The operator is responsible for other people in the work area. The operating company make the instruction manual accessible to the operator and make sure, that the operator have read and understand the instruction manual.
Unauthorized modifications to the device are forbidden due to safety reasons. When replacement damaged parts of the device only original spare parts must be used.
You should always activate these two safety precautions, when the
Microtome is not in use.
The handwheel brake – if activated – keeps the handwheel in its position
and protect the operator from crushing hazards.
Don’t use the device in an explosive area.
The knife carrier comes with a finger protection, which always should be
used to decrease the risk of injury.
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 4 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
2. Information about Microtome M530
2.1. Shipment
The shipment includes:
• 1x Microtome M530,
• 1x Cover for the Microtome M530,
• 1x power cord 230V / 115V,
• 1x Tool kit,
• 1x Low profile blade holder,
• 1x TissueCut Microtome Blade,
• 1x Waste tray,
• 1x Standard clamp,
• 1x instruction manual for the Microtome M530.
2.2. Technical Data
Fine feed steps setting values: 1 – 99 in 1 µm sequences
Coearse feed steps setting values: 5 - 100 in 5 µm sequences
100 –500 in 50 µm sequences
DImensions incl. Tray (L/W/H): 530 x 377 x 281 mm
Weight: 26 kg
Voltage: 110 ... 240 VAC / 50/60 Hz
Fuse: 2x T1.6 A
2.3. Transport
When unpacking, carrying or moving the Microtome M530, use the recessed handles, which
are positioned at the front and at the back of the unit.
Do never use the knife holder or the section waste tray as handle for
carrying!
Before every transport remove the knife holder and the section waste tray!
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 5 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
Remove the section waste tray by pulling it to the front and the knife holder by unlocking it
and also pulling it away to the front.
Carry handles
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 6 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
2.4. Overview components of the Microtome M530
2.4.1. Front view
Handwheel brake
Handwheel
Knife carrier
Multiple functional
knob for change
section thickness
Section waste tray Specimen clamping
system
Film keyboard
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 7 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
2.4.2. Back Site
2.4.3. Film Keyboard
Counter
Type plate
Power Connector with fuses
Reset Counter
Buttons for saving profils
Trim / Fine Mode Actual Section Thickness
Position of object feed Automatic Retraction
On / Off
Automatic Retraction
Trim
Objection feed foward Objection feed backward
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 8 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
2.4.4. Knife carrier
2.4.5. Object clamp
Cassette clamp
Lever for fix and lose
the specimen
finger guard
Lever for fix and lose
the angle adjustment
Lever for fix and lose
the blade
Lever for fix and lose
the blade carrier
Lever for fix and lose
the knife carrier
Screws for angle
adjustment
Lever for fix and lose
the casette clamp
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 9 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
3. Startup the Microtome M530
3.1. Preface
3.2. Mounting
3.3. Dismounting
The Microtome M530 must be installed on a stable, level and horizontally
oriented base to ensure the safe and reliable operation.
Before start to work on the Microtome M530, leave the unit on the
designated workspace for at least 2 hours or more, to adjust to room
temperature.
Remove the microtome blade and activate the handwheel brake, when
dismounting the Microtome M530!
To Transport the Microtome M530, use the carry handles. For more
information, see chapter 2.3 – Transport.
To Transport the Microtome M530, use the carry handles. For more
information, see chapter 2.3 – Transport.
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 10 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
4. Operation
4.1. Power On
Check, if the on / off switch is in the „0“ (off) position. If not, press the switch to the „0“
position. Plug in the power cord and press the on / off switch in the „I“ (on) position. The
Microtome M530 is ready for operation, if the display of the film tastatur light on.
4.2. Prepare the specimen
4.2.1. Object orientation
The angle of the cassette clamp can adjust to 8° in all directions. To adjust the angle, release
the cassette clamp by turning the lever (3) in your direction. After this you can rotate the
cassette clamp for 360° and you can adjust the angle by turning the angle-adjustment
screws (1, 2). Screw (1) is for the vertical adjustment, screw (2) for the horizontal
adjustment.
When finished the adjustment, fix the cassette clamp by turning the lever (3) in the fix-
position.
Always release the lever (3) before adjusting the angle adjustment. If not,
the object clamp can be damaged.
Make sure the contact surfaces of the cassette are free from paraffin to
achieve an optimal clamping.
After a long period of non-use, pull of the plug in to protect the device by lightning strike or something near it.
3
1
2
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 11 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
4.2.2. Insert the specimen
To insert a specimen, pull the lever (1) in your direction, and then insert the specimen in the
cassette clamp (2).
To remove the specimen, press the lever (1) in your direction and remove the specimen.
4.2.3. Remove the Cassette clamp
If you want to install another cassette clamp, turn the lever (1) in your direction.
After this remove the complete lever by pulling out the the right side. Now you can remove
the cassette clamp. Notice that the cassette clamp must be in center before you remove it.
Insert the new cassette clamp, notice, that the hole of the adapter need to fit with the hole
from the object clamp. After this, insert the lever in the object clamp and fix it by pressing the
lever in the fix-position.
Turn the cassette clamp in the center position, before you start to remove
the cassette clamp.
The screws of the angle adjustment have a lock function for center the
cassette clamp. If you will center the cassette clamp turn the both screws as
long as they locked. When both screws locked, the cassette clemp is in the
center.
1
2
1
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 12 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
4.3. Prepare knife carrier
4.3.1. Inserting the microtome blade
To insert the blade, turn the finger guard to the front and lose the insertion for the blade by
pressing the lever (1) to the front. Now the insertion for the blade will open.
The gap from the blade carrier will open.
Now insert the microtome blade carefully in the gap (2)! Press the lever (1) back to fix the
microtome blade.
The knife carrier has ergonomic grips, which can be set in each position. To
positioning the grips pull the grips careful out and turn it in the new position.
Lose the grips for lock.
After every operation or non-use, put the finger guard over the microtome
blade and activate the brake for the handwheel!
1
2
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 13 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
4.3.2. Adjustment of the knife carrier
The complete knife carrier can be moved forward and backward on the rails or taken off the
rails. Pull up the lever (1) to release the knife carrier. Turn the knife carrier in the new
position and pull down the lever (1) to fix the knife carrier.
4.3.3. Adjustment of the blade holder
The blade holder can be moved in the right or left direction. Pull down the lever (1) to release
the blade holder. Turn the blade holder in the new position and pull up the lever (1) to fix the
blade holder.
1
1
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 14 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
4.3.4. Adjusting The Knife Carrier Angles
The knife carrier has a scala for the angle-adjustment on the left side (2). The angle can
adjust from 6 to 12 degree.
Pull down the lever (1) to release the knife carrier. Turn the knife carrier in the new position
and pull up the lever (1) to fix the blade holder.
4.3.5. Change the microtome blade
To insert the blade, turn the finger guard to the front and lose the insertion for the blade by
pressing the lever (1) to the front. Now the insertion for the blade will open.
Take the cleaning brush - the brush has an integrated magnet on the back, which simplifies
the removal of the microtome blade. Move the back of the cleaning brush to the microtome
blade until the magnet adheres to the blade and pull the blade carefully out of the blade
holder head.
.
2
1
1
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 15 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
Put the microtome blade in the knife carrier head and press up the lever (1).
4.4. Use the handwheel brake
Tip the lever from “open“ position to “close” position. The lever will automatically move to
“close“ position. Avoid to overwinding the lever to the mechanical end position.
Turn down the lever to release the handwheel.
Do not rotate the handwheel while the brake is activated. The device can be
damaged!
Handwheel not fixed Handwheel fixed
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 16 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
4.5. Start the cutting process
4.5.1. Trimming the specimen
Without turning the handwheel tip button (8) to approach the specimen to the blade in min
5µm steps or in actual user selected trim value (example 20µm) . One tip one movement to
the specimen. Acustic signal confirmed action.
Hold pressing button (8) and turn the handwheel each turn moves the specimen min 5µm or
user selected trim value to the blade.
Use a different area of the cutting edge for trimming than for sectioning by
moving the blade holder left or right.
After every operation or non-use, put the finger guard over the microtome
blade and activate the brake for the handwheel!
Before the cutting process starts, the knife carrier and specimen clamp
needs to adjusting. For more Information, read chapter 4.2 Prepare the
specimen and chapter 4.3 Prepare the knife carrier.
1
3
4
6
2
5
7
8
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 17 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
If you want to move the specimen away from the blade tip button (6) each tip moves the
specimen 6µm away from the blade. Hold pressing button (6) until second acustic signal
appears moves the specimen automatically into home position. You can stop that
automatically movement by again tip button (6).
Check if "Trim" is shown on the display. Set the slice thickness by turning the multiple
functional knob (Example 50 µm). The display shows the currently set cut thickness.
Cut the sample by turning the hand wheel until the desired object area is reached.
4.5.2. Sectioning the specimen
Check if "Fine" is shown on the display. Set the section thickness by turning the multiple
functional knob (Example 5 µm). The display shows the currently set cut thickness.
Cut the sample by turning the hand wheel until the desired object area is reached.
4.5.3. Automatic Retraction
To prevent the knife and the specimen from damage when the object clamp is moving up again, the specimen can be pulled back during upward movement by activating the automatic “Retraction”. The “Retraction” can be switched on and off by pressing button (2).
4.5.4. Position Memory
The M530 offers the possibility to store 3 values for trimming positions. These values can be used to recover certain positions within a short time. A position can be stored by pushing one of the button (3), (4) or (5) for more than one second until a signal appears. The value of the actual positioning then is stored in your chosen memory and can be approached any time later on by just pushing the memory key again. Occupied memory numbers are shown “M1 … M3” including their values in the display to avoid an overwriting of your positions. Of course you can overwrite your values just by proceding the same way again.
If the function is activated there is an “R” appearing in the display.
Press the multiple functional knob to switch between the trim and fine-cut
mode.
Press the multiple functional knob to switch between the trim and fine-cut
mode.
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 18 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
4.6. Troubleshooting
Problem Possible Reason Knife Carrier cannot be
fixed
Clamping lever has to be readjusted
Cutting movement (rotation)
too hard
Guiding rolls dirty or defective, call MEDITE service for help
Piston can not be driven
forwards or backwards
Thrust / pullback-motor are defect. Call MEDITE service for help
Device does not turn on Fuse defect. Change the fuse (see chapter 4.6.1 – Change the
Fuse). If the problem is still exists, call MEDITE service for help.
Screen does not turn on
Display defect. Call MEDITE service for help
Keypad buttons do not
respond / react incorrectly
Film tastatur defect. Call MEDITE service for help
4.6.1. Change the Fuse
To change the fuses switch-off the device. Press the on / off switch in the "0" position and
remove the power cord. Then squeeze the two pins (1) and pull the drawer (2) with the
fuses out carefully.
In chapter 2.2 - Technical Data are the data of the fuse. Replace the defect fuse (3) with a
new fuse and slide the tray back into the appropriate slot until you hear a clear click.
After each restart of the Microtome these values are deleted for safety.
1
2
3
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 19 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
5. Cleaning, Maintenance and Recycling
5.1. Cleaning Instructions
The Microtome M530 is a precision instrument. Of course it is necessary to keep it clean to
guarantee a long timespan between maintenance checks. So, after each use, you have to
clean the specimen clamp, the knife carrier, the section waste tray and all visible guiding.
The surface of the Microtome may be cleaned with standardized, not scratching cleaning
agents, if needed. To remove sticking deposits of sections, use a brush. Don’t use sharp-
edged blades or tools!! These will destroy the surface of the Microtome. The whole
Microtome has to be checked regularly by MEDITE service.
5.2. Timing of Cleaning And Maintenance
Activity daily Every week Every 12
months
Clean the Microtome from remains of
sections and wax as explained in point 4.1 X
Check for all guiding, locking- and clamping-
levers to work perfect X
Recommendation:
Contact you responsible MEDITE
representative or Service for maintenance
of the hidden parts.
X
Before starting the cleaning, always switch-off the device and pull of the power cord.
A regularly maintenance of the Microtome M530 (cleaning and greasing of
the relevant mechanical parts, cleaning of parts inside and exchange of
expandable parts) by MEDITE Service is the main requirement for a well
working microtome! Ask your local MEDITE representative for a
maintenance check.
MEDITE recommends you to include a daily cleaning procedure – after
finish of routine work - into your cleaning and maintenance plan.
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 20 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
5.3. Abandonment / Recycling
After expiration of instrument’s life span please transfer unit into professionally disposal or
send it back to MEDITE. As a manufacturer / distributor we take back the used equipment,
requiring a payment for disposal.
Instruction manual Microtome M530
MEDITE GmbH . Wollenweberstraße 12 . 31303 Burgdorf . Germany 21 Phone +49 5136 88 84 0. Fax +49 5136 88 84 55. [email protected] . www.MEDITE.de
6. Notice
MEDITE GmbH
Wollenweberstraße 12
31303 Burgdorf
Germany
Phone +49 5136 88 84 0
Fax +49 5136 88 84 55
[email protected] · www.MEDITE.de