instruction manual for spectro-uv11/v12/uv12

PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION

3, Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529 [email protected]

MRC.VER.02-11.10

INSTRUCTION MANUAL FOR

SPECTRO-UV11/V12/UV12 Spectrophotometers

Contents

Safety ……….……………………………………………………………………. 1111

General ………………………………………………………………………. 1

Electrical …………………………………………………………………….. 1

Warning ………………………………………………………………………… 2

Working Principle ………………………………………………………… 2

Unpacking Instructions …………………………………………………… 3

Specifications ……………………………………………..……………… 3

Installation …………………………………………………………………… 3

Operation ………………………………………………………..……………… 4

Prepare the Spectrophotometer ………………………….….…………… 4

Description of keys ………… ………………….….……..………… 5

Turn on spectrophotometer … ……………………….……..……………… 5

Basic operation……………………………………….…….……………… 6

Analyse Sample …………………………………………………………… 9

Basic Mode ………………………………………………….…..…….. 9

Quantitative-A (Only For V12/UV12) ………………………………….. 10

Coefficient……………………..…………………………………… 11

Standard Curve…………..………………………………………… 13

Quantitative-B(Only For UV11)…..………………………………….. 17

Maintenance ……………………………………………………………..……. 19

Lamp Replacement………………………………………………….…… 19

Replace W lamp……………………………….…………………… 19

Replace D2 lamp………………………………………………….. 20

Battery Replacement…………………………………………………….. 21

Troubleshooting……………………………………………….………………… 22

1

Safety: The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT, 1974. Read the following before installing and using the instrument and its accessories. The UV11 and 12 Series should be operated by appropriate laboratory technicians.

General: The apparatus described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory. For the correct and safe use of this apparatus it is essential that laboratory personnel follow generally accepted safe procedures in addition to the safety precautions called for in this manual. The covers on this instrument may be removed for servicing. However, the inside of the power supply unit is a hazardous area and its cover should not be removed under any circumstances. There are no serviceable components inside this power supply unit. For UV11 and 12 Series, avoid touching the high voltage power supply at all times. Some of the chemicals used in spectrophotometer are corrosive and/or inflammable and samples may be radioactive, toxic, or potentially infective. Care should be taken to follow the normal laboratory procedures for handling chemicals and samples.

Electrical: Before switching on the apparatus, make sure it is set to the voltage of the local power supply. （The Voltage Switch is in the rear of the instrument, if you want to change the voltage, you must remove the cover of the instrument first）. See Fig. 1-1.

Fig. 1-1

The power cord shall be inserted in a socket provided with a protective earth contact. The protective action must not be negated by the use of an extension cord without a protective conductor.

Warning: Any interruption of the protective conductor inside or outside the apparatus or

Voltage Switch

2

disconnection of the protective earth terminal is likely to make the apparatus dangerous. Intentional interruption is prohibited. Whenever it is likely that the protection has been impaired, the apparatus shall be made inoperative and be secured against any unintended operation. NEVER touch or handle the power supply on UV11 and 12 Series due to the high voltage. The protection is likely to be impaired if, for example, the apparatus � Shows visible damage � Fails to perform the intended measurements � Has been subjected to prolonged storage under unfavorable conditions � Has been subjected to severe transport stresses

Working Principle: The spectrophotometer consists of five parts: 1) Halogen or deuterium lamps to supply the light; 2) A Mono-chromator to isolate the wavelength of interest and eliminate the unwanted second order radiation; 3) A sample compartment to accommodate the sample solution; 4) A detector to receive the transmitted light and convert it to an electrical signal; and 5) A digital display to indicate absorbance or transmittance. The block diagram (Fig 1-2) below illustrates the relationship between these parts. Block diagram for the Spectrophotometer

Light Mono- Sample Detector Display

Source chromator Compartment

Fig1-2

In your spectrophotometer, light from the lamp is focused on the entrance slit of the mono-chromator where the collimating mirror directs the beam onto the grating. The grating disperses the light beam to produce the spectrum, a portion of which is focused on the exit slit of the mono-chromator by a collimating mirror. From here the beam is passed to a sample compartment through one of the filters, which helps to eliminate unwanted second order radiation from the diffraction grating. Upon leaving the sample compartment, the beam is passed to the silicon photodiode detector and causes the detector to produce an electrical signal that is displayed on the digital display.

Unpacking Instructions: Carefully unpack the contents and check the materials against the following packing list to

100%T 0 Abs

3

ensure that you have received everything in good condition.

Packing List Description Quantity

� Spectrophotometer ............................................................... 1

� Mains Lead ........................................................................... 1

� Cuvette ............................................................................. Set of 4, glass

� Set of 2, quartz

� Manual .................................................................................. 1

Note: V12 includes only glass cuvette, no quartz cuvette.

Specifications:

Installation: 1. After carefully unpacking the contents, check the materials with the packing list

(page 4) to ensure that you have received everything in good condition.

2. Place the instrument (Fig.1-3) in a suitable location away from direct sunlight. In order to have the best performance from your instrument, keep it as far as

Model UV11 V12 UV12 Wavelength Range 200-1000nm 325-1000nm 200-1000nm Spectral Bandwidth 4nm Optical System Single Beam, Grating 1200lines/mm Wavelength Accuracy ±2nm ±1nm Wavelength Repeatability 1nm 0.5nm Wavelength Resolution 1nm Photometric Accuracy ±0.5%T Photometric Repeatability ±0.3%T Photometric Range -0.097-1.999A

0-125%T -0.097-2.5A, 0-125%T

Stray Light ≤0.3%T Stability ±0.004A/h @500nm ±0.002A/h @500nm Display Graphic LCD (128X64 dots) Keyboard Membrane keypad Photometric Mode T, A, E Detector Si Photodiode

Sample Compartment Standard 10mm path length cuvette

Accommodates 100mm path length cuvette with optional holder Light Source Tungsten & Deuterium Tungsten lamp Tungsten & Deuterium Output USB Port Parallel Port (printer) Power Requirement AC 220V/50Hz or 110V/60Hz Dimensions (W x D x H) 480 x 360 x 160mm Weight 12kg 11kg 12kg

4

possible from any strong magnetic or electrical fields or any electrical device that may generate high-frequency fields. Set the unit up in an area that is free of dust, corrosive gases and strong vibrations.

Fig.1-3

3. Remove any obstructions or materials that could hinder the flow of air under and around the instrument.

4. Use the appropriate power cord and plug into a grounded outlet.

Fig.1-4 Rear panel

5. Turn on your UV11 and 12 Series model spectrophotometer. Allow it to warm up for at least 20 minutes before taking any readings.

Operation: Prepare the spectrophotometer Fig 1-5 is the control panel. User can perform all operations by pressing the keys and all the results and operation information are displayed on the LCD.

LCD & PANEL

COMPARTMENT

HOLDER CHANGER KNOB

FAN

POWER SWITCH

POWER CORD INLET

FUSE USB PORT

PARALLEL PORT

CONTRAST

5

Edit keys Scroll keys Fig 1-5

� Description of keys 【GOTO λ】 Set wavelength; 【ZERO】 Blank 【SET】 System function 【PRINT】 Print or delete the test results 【--】 Instruction selection keys, the instruction will display on the bottom of

the screen. The position of the keys is corresponding to the position of the instructions listed on the screen.

Note：：：：To describe conveniently, we call the left key of 【【【【----】】】】 as “F1” ，，，，and the right key of 【【【【--】】】】 as “F2” in this manual.

【∧】,【∧】 Scroll items for selecting; Browse the items for selection;

� Turn on spectrophotometer 1) Turn on spectrophotometer by pressing the Power Switch (I/O). 2) A welcome interface will display on the screen. Then the instrument starts to self-test. 3) When self-testing ended, it goes into the main menu. (Fig. 1-6)

Note: DO NOT OPEN SAMPLE COMPARTMENT LID DURING SELF-TESTING.

Select key

6

Fig. 1-6 Main menu

Basic Operation The display interface is user-friendly, always you can complete all the operation according to the clues on the screen.

1) Set Wavelength Press 【GOTO λ】 to go into wavelength setting interface. Then press 【∧】or【∧】to select the wavelength you want, then press OK （F1）to confirm. After the wavelength being changed, it returns to main menu automatically. If you don’t want to change the wavelength, press Return (F2) to go back to main menu. (Fig. 2-1) 【GOTOλ】 Return

OK

Fig.2-1

2) Blank Pull the reference cuvette into the light path, Press 【zero】 to blank. (Fig.2-2) 【ZERO】 Fig.2-2 Blank

3)Utility Press 【SET】to go into system functions interface, use 【∧】or【∧】to select, then press

WL：：：：546.0nm 0.000Abs 100.0 %T

Basic Quantitative

Instrument Self-test……

WL：：：：546.0nm Please Enter WL.:

576 nm OK Return

WL：：：：546.0nm 0.000Abs 100.0 %T

Basic Quantitative

WL：：：：576.0nm zero…

100.0 %T Basic Quantitative

WL：：：：576.0nm 0.000Abs 100.0 %T

Basic Quantitative

WL：：：：576.0nm 0.000Abs 100.0 %T

Basic Quantitative

7

ok (F1) to go into the corresponding item. (Fig.2-3) 【SET】 Cancel CONTINUOUS

Fig.2-3 go into utility setting interface

3.1 Test Mode Move the cursor on “Test Mode”, followed with ok (F1) pressed, it goes into the mode selection interface.（Fig.2-4） OK Cancel

Fig. 2-4 select test mode Move the cursor on the photometric mode you want by pressing 【∧】or【∧】, then press ok (F1) to confirm. Once the symbol “√√√√” appears at the end of the item, you can press Cancel ( F2) to return to the upper interface. 3.2 Deuterium Lamp Management (only for uv 11 and uv12 ) Move the cursor on “D2 Lamp Off”, followed with ok (F1) pressed, it goes into another interface. (Fig. 2-5) OK Cancel Fig. 2-5 Deuterium lamp management Choose the deuterium lamp condition you want, press ok (F1) to confirm, then press

●●●●1. Test Mode ○○○○2. D2 Lamp Off ○○○○3. W Lamp On OK Cancel

WL：：：：576.0nm 0.000Abs 100.0 %T

Basic Quantitative

●●●●4. Dark Current ○○○○5. Reset WL. ○○○○6. Version OK Cancel

●●●●1. Test Mode ○○○○2. D2 Lamp Off ○○○○3. W Lamp On OK Cancel

●●●●1. Abs. √√√√ ○○○○2. T% ○○○○3. Energy OK Cancel

○○○○1. Test Mode ●●●●2. D2 Lamp Off ○○○○3. W Lamp On OK Cancel

√√√√ D2 Lamp Off ●●●●1. On ○○○○2. Off √√√√ OK Cancel

8

Cancel (F2) to return to upper interface. Note: When the D2 is from “off” to “on”, it will cost a few minutes to warm up.

3.3 W Lamp Management Move the cursor on “W Lamp On”, followed with ok (F1) pressed, it goes into another interface. (Fig. 2-6) OK Cancel Fig. 2-6 D2 lamp manage Choose the W lamp condition you want, press ok (F1) to confirm, then press Cancel to return to upper interface.

3.4 Dark Current Move the cursor on “Dark Current ”, followed with ok (F1) pressed to get dark current. (Fig.2-7) Once the environment changed, such as temperature, instrument’s station, etc, you should going this operation. OK Auto Fig. 2-7 Get dark current 3.5 Reset WL . Move the cursor on “Reset WL .”, then press OK (F1), system begins to reset wavelength. (Fig.2-8) OK Auto Fig. 2-8 Calibrate Wavelength 3.6 See the version Move the cursor on “Version ”, then press OK (F1), you’ll find the version of this instrument’s hardware and software. (Fig.2-9) Press any key to return.

○○○○1. Test Mode ○○○○2. D2 Lamp Off ●●●●3. W Lamp On OK Cancel

√√√√ W Lamp On ●●●●1. On √√√√ ○○○○2. Off OK Cancel

●●●●4. Dark Current ○○○○5. Reset WL. ○○○○6. Version OK Cancel

Get Dark Current.

○○○○4. Dark Current ●●●●5. Reset WL. ○○○○6. Version OK Cancel

Calibrating WL….!

9

OK Any key Fig. 2-9 Version

Analyze Sample Before measurement Make a blank reference solution by filling a clean cuvette half full with distilled or de-ionized water or other specified solvent. Wipe the cuvette with tissue to remove the fingerprints and droplets of liquid. Fit the blank cuvette into the 4-cell linear changer and place the cuvette in the slot nearest you. For the UV11 and 12 Series, push the changer so that the cuvette is in the light path (Push the rod in). Close the lid. We provide two test methods in UV11 and 12 Series, they are basic mode and quantitative mode.

1. Basic Mode Firstly, set the wavelength you need (Ref. Page 7).

1) Go into basic test mode Pull the blank cuvette into the light path, then press Basic (F1). After automatic blanking,

it goes into Basic test interface. (Fig. 3-1) Basic Cancel Fig.3-1 go into Basic test Interface 2) Begin to test Pull the sample into the light path, then press Test (F1) to measure. The test result displays on the screen. And it will be numbered automatically. (Fig.3-2)

Test Cancel Fig.3-2 Basic test

WL：：：：546.0nm 0.000Abs No. WL. T% Abs Test Cancel

WL：：：：546.0nm 0.000Abs 100.0 %T

Basic Quantitative

WL：：：：546.0nm 0.000Abs No. WL. T% Abs Test Cancel

WL：：：：546.0nm 0.000Abs No. WL. T% Abs 1 260 100.0 0.000 2 330 100.0 0.000 Test Cancel

○○○○4. Dark Current ○○○○5. Reset WL. ●●●●6. Version OK Cancel

TYPE: SPECTRO-UV12 SOFTWARE: V2.1.0 HARDWARE: V1.2

10

Repeat step 2) to measure other samples, the results will be numbered orderly. Total 200 groups of test data can be saved in the RAM, 3 groups per screen. Use 【∧】,【∧】keys to scroll. Press Cancel 【F2】to return to the main menu.

3) Clear or Print the data Press the key of 【PRINT】, it displays as follow: (Fig.3-3) 【PRINT】 Cancel Fig.3-3 print or clear Move the cursor on the item you want, then press Ok (F1) to confirm. Item 1 means all the data will be deleted after printing. Item 2 means all the data will be deleted from screen and RAM without printing. Item 3 means returning to upper interface without any choice, also you can press Cancel (F2) to return Note: You can also Set wavelength and Blank by pressing 【GOTO λ】 and【ZERO】

individually in the Interface of Basic Test.

2.Quantitative-A ( Only for UV/V 12) Press Quantitative （F2）to go into quantitative test main menu. Three items are under your choice. (Fig.3-4) You can create, load and delete curves. Quantitative Cancel Fig.3-4 go into quantitative Interface 2.1 Create curve

Use 【∧】and 【∧】to move the cursor on the first item, then press OK (F1) to confirm. You have two methods to choose. (Fig.3-5) ok Cancel Fig.3-5 go into method choice Interface

1. Print and Clear 2. Only Clear, Not Print 3. Cancel OK Cancel

WL：：：：546.0nm 0.000Abs No. WL. T% Abs 1 260 100.0 0.000 2 330 100.0 0.000 Test Cancel

●●●●1.Create Curve ○○○○2.Load Curve ○○○○3.Remove Curve Ok

Cancel

WL：：：：546.0nm 0.000Abs 100.0 %T

Basic Quantitative

WL.:546.0nm ●●●● Coefficient ○○○○ Standard Curve Ok Cancel

●●●●1.Create Curve ○○○○2.Load Curve ○○○○3.Remove Curve

Ok Cancel

11

If you have known a curve’s equation, you can choose “Coefficient” method. Input all the coefficients to complete the equation, then to measure unknown samples. If you don’t know the equation, you’ll have to create a standard curve by standard samples. At most 9 samples can be used. 2.1.1 Coefficient

1) Move the cursor on “Coefficient ”, then press ok (F1), it goes into the wavelength setting interface automatically.(Fig.3-6),

ok

Cancel

Fig.3-6

2) choose the wavelength you need by pressing 【∧】and 【∧】,followed with ok (F1) pressed. It goes into the coefficient setting interface. (Fig. 3-7)

ok

Cancel

Fig.3-7

3) Input the coefficient K There are five zeros on the screen, the cursor is on the first one at first. Press 【∧】 or 【∧】, the first zero will vary between “0 ~9 “. Choose the number or symbol you need, then press ok (F1) to confirm, the cursor then moves to the next zero automatically. The second to the fifth bits vary between “0~9” and decimal point. Complete other bits of Coefficient K in the same way.


WL.:546.0nm Work WL.:

546 nm

Ok Cancel

WL.:440.0nm Coefficient K: 0 0 0 0 0 Ok Cancel

WL.:546.0nm

Wavelength：：：：

440 nm Ok Cancel


12

4) Input the coefficient B When the last bit of K value being confirmed and Ok (F1) pressed, the interface goes into the Coefficient B setting interface. (Fig. 3-8)

ok

Cancel

Fig.3-8

The first bit is symbol bit, vary from “+” to “-“, other bits are number bits. The way to Input B is the same as K does. After the last bit of B being confirmed, the curve will display on the screen. (Fig.3-9)

ok

Cancel

Fig.3-9

5) Begin to test When the curve displays on the screen, you can use it to test the unknown samples’ concentration. Just Press Test to do it. (Fig.3-10)

WL.:440.0nm Input Coefficient B + 0 0 0 0 0 Ok Cancel

WL.:440.0nm Input Coefficient K 0 1 5 . 8 Ok Cancel

WL.:440.0nm Input Coefficient B + 0 . 0 0 2 Ok Cancel

0.500A

r=0.999 0 0.8C

C=15.8*A+0.002 Test Cancel



13

Test

Cancel

Fig.3-10

6) Print Press 【Print 】in the concentration measure interface, the test results will be printed. (Ref. P.10)

2.1.2 Standard Curve

1) Insert Blank Reference Move the cursor on “Standard Curve”, then press ok (F1). The system will ask you to

insert the blank reference. (Fig.3-11), a. Pull the blank cuvette in the light path, then close the compartment lid. b. Press 【GOTO λ】to set wavelength, Ref. Page 6. c. Press Ok (F1) to blank.

ok

Cancel

Fig.3-11

2) Input the number of standards After blanking, the system will ask you to input the number of the standards. Use 【∧】 or 【∧】to choose the number you need, then press Ok (F1) to confirm. Note: At most 9 standards are permitted. Other number is in valid. (Fig. 3-12)

0.500A

r=0.999 0 0.8C C=15.8*A+0.002 Test Cancel

WL：：：：440.0nm 0.000Abs No WL Abs Conc 1 440 0.010 0.160 2 440 0.020 0.318 3 440 0.040 0.634 Test Cancel


WL.:546.0nm ○○○○Coefficient ●●●●Standard Curve Ok Cancel

WL.:546.0nm 0.000Abs Please Insert Blank: Ok Cancel

14

ok

Cancel

Fig.3-12

3) Input the concentration of standards When you have finished the last step, the system will ask you to input the standards’ concentration one by one. See the below figure 3-13.

ok

Cancel

Cancel

Fig. 3-13

You should input the No.1 standard sample’s concentration first as the screen indicates. Please Ref. Page 12- Input the coefficient B , the inputting way is the same. 4) See the curve When the last one is finished, for example total 6 standards are used, the curve will be drawn on the screen, and the curve equation will also display on the screen. (Fig.3-14)

WL.:546.0nm 0.000Abs Please Insert The Blank Sample! Ok Cancel

WL.:546.0nm 0.000Abs Number: 3 Ok Cancel

WL.546.0nm 0.000Abs Insert 1 # Standard Input 1 # Conc: 0 0 0 0 0 Ok Cancel

WL.:546.0nm 0.000Abs

The Number of the

Standards: 6 Ok Cancel



15

ok

Cancel

Fig.3-14

The curve and the curve equation will be saved in the RAM automatically, total 200 curves can be saved, and they’ll be numbered orderly. 5) Test Insert the unknown concentration sample in the holder, then close the compartment lid and pull the sample in the light path. Press Test (F1) to measure. The results will display on the screen. (Fig. 3-15)

Test

Cancel

Fig. 3-15 6) Print You can print the test results and the curve, just press 【Print 】. Please Ref. Page 11 .

2.2 Load Curve

Use 【∧】and 【∧】to move the cursor on the second item, then press OK (F1) to confirm. (Fig.3-16), you’ll go into the curve choice interface.

WL.546.0nm Insert 1 # Standard Input 1 # Conc: 0 0 . 1 8 Ok Cancel

0.500A

r=0.999 0 0.8C C=1.0*A+0.001 Test Cancel

0.500A

r=0.999 0 0.8C C=1.0*A+0.001 Test Cancel




16

ok Cancel

Fig.3-16 go into curve choice Interface

All the curves you create will be saved in the RAM automatically, you can select the one you want using 【∧】and 【∧】followed with Ok (F1) pressed.

Note: Total 200 curves can be saved, you can find them by pressing 【∧】and 【∧】.

ok

Cancel

Cancel Test

Fig.3-17 2.3 Delete Curve

Use 【∧】and 【∧】to move the cursor on the third item, then press OK (F1) to confirm. (Fig.3-18), you’ll go into the curve deleting interface. ok Cancel

Fig.3-18 Go into curve choice Interface

Move the cursor on the equation you want to remove, then press Ok (F1), the curve will be deleted from the screen and RAM.

No: C=K*A+B 1. C=0.860××××A+0.000 * 2. C=0.732××××A+0.125 3. C=0.005××××A+0.002 <

OK Cancel

○○○○1.Create Curve ●●●●2.Load Curve ○○○○3.Remove Curve

Ok Cancel

0.500A

r=0.999 0 0.8C C=0.860*A+0.000 Test Cancel


No: C=K*A+B 1. C=0.860××××A+0.000 * 2. C=0.732××××A+0.125 3. C=0.005××××A+0.002 <

OK Cancel

No: C=K*A+B 1. C=0.860××××A+0.000 * 2. C=0.732××××A+0.125 3. C=0.005××××A+0.002 <

OK Cancel

○○○○1.Create Curve ○○○○2.Load Curve ●●●●3.Remove Curve

Ok Cancel

17

2.Quantitative-B (Only for UV 11)

This is a simple quantitative method, always is called Single Point Standard Curve Method . If you have a standard sample, and it’s Absorbance can be measured, then a curve can be created through zero. And you can use the curve to measure other samples. For UV11, the curve will not display on the screen, but you can use it to measure. Once the Quantitative (F2) be pressed, system will ask you to insert the blank first. (Fig.3-19) Quantitative Cancel

Fig.3-19 go into quantitative Interface

You should operate as the follow steps: 1) Insert the Blank Reference, standard sample and unknown sample cuvettes into the

changer slots, then close the compartment lid. 2) Pull the blank cuvette in the light path, Press 【GOTO λ】 to set wavelength, please

Ref. Page7. 3) Press Ok (F1) to blank. Then system asks you to input the standard’s concentration.

(Fig. 3-20) Ok Cancel Fig. 3-20

4) Pull the standard cuvette in the light path, then input the value of the concentration.

WL.:546nm ●●●● Coefficient ○○○○ Standard Curve Ok Cancel

WL：：：：546.0nm 0.000Abs 100.0 %T

Basic Quantitative


WL.:546.0nm 3.000Abs Please Insert Blank: Ok Cancel


WL：：：：546.0nm 0.000Abs

Please Insert Blank: Ok Cancel


WL.:546.0nm 3.000Abs Please Insert Standard: Input Conc.: 0 0 0 0 0 Ok Cancel

WL：：：：546.0nm 0.000Abs 100.0 %T

Basic Quantitative

18

There are five zeros on the screen, the cursor is on the first one at first. Press 【∧】 or 【∧】, the first zero will vary between “0 ~9 “. Choose the number you need, then press ok (F1) to confirm, the cursor then moves to the next zero automatically. The 2nd ~5th bits vary between “0~9” and decimal point. Complete other bits of the concentration value in the same way. 5) When you have finished the 5th bit, press Ok (F1) to go into the test interface. (Fig.

3-21)

Ok Cancel

Fig. 3-21 Go into test interface 6) Push the blank cuvette into the light path, press【ZERO】to blank. Then pull the unknown sample cuvette into the light path, it’s concentration will display on the screen.

WL.:546.0nm 3.000Abs Please Insert Standard: Input Conc.: 1 . 0 0 0 Ok Cancel

WL.:546nm 3.000Abs Please Insert Standard: Input Conc.: 1 . 0 0 0 Ok Cancel

WL.:546.0nm 0.000Abs

0.000 Conc.

Cancel

WL：：：：546.0nm 0.000Abs 100.0 %T

Basic Quantitative

19

Maintenance Inside Structure

Fig.4-1 Inside Planform 1. Lamp Replacement A. Replace Tungsten-Halogen Lamp 1. Turn off and unplug the instrument. 2. Remove the four screws on the sides of the spectrophotometer. 3. Remove the Cuvette Holder Control Knob by unscrewing the rod counterclockwise. 4. Remove the cover of the instrument very carefully and place it in the right of the

instrument. BE SURE NOT TO PULL PANEL WIRING LOOSE!

5. Unscrew the two screws from the Lamp Chamber and remove its cover. (Caution: The cover may be HOT ，，，，be sure to wear gloves when act)

6. Unplug and remove the lamp from ceramic base (the black connector). Insert the new lamp; pushing it in as far as it will go.

Fig.4-2

Compartment

Cell-holder

Mono-chromator Chamber

Fan

Lamp Chamber Radiator

Tungsten-Halogen Lamp

20

CAUTION: 1) do not handle the lamp with bare fingers. Use tissue or cloth when handling lamp. 2) There’s no difference in polarity of the two legs of Tungsten-halogen lamp. 7. Switch the instrument on. Set the wavelength at 340 nm, insert an empty cuvette,

and blank the instrument. If the energy is low, adjust the lamp by “pulling” or “pushing” it so that the light beam is focused on the entrance slot of the mono-chromator. Since the lamp socket is pre-aligned, there will be minimum, if any, adjustment required.

8. Switch the instrument off, re-cover the lamp chamber and tighten the two screws. 9. Reinstall the instrument cover by positioning the front of the cover first and then sliding

the back of the cover over the backside grill plate. Be sure to prevent all wires from being pinched in the process.

10. Reinstall the four screws and the Cuvette Holder Control Knob . B. Replace Deuterium Lamp WARINING: Wear UV protection Glasses before changing the Deuterium Lamp! 1. Turn off and unplug the instrument (VERY IMPORTANT: HIGH VOLTAGE ). 2. Remove the four screws on the sides of the spectrophotometer. 3. Remove the Cuvette Holder Control Knob by unscrewing the rod counterclockwise. 4. Remove the cover of the instrument very carefully and place it in the right of the

instrument. BE SURE NOT TO PULL PANEL WIRING LOOSE!

5. Unscrew the two screws from the Lamp Chamber and remove its cover.

(Caution: The cover may be HOT ，，，，be sure to wear gloves when act)

6. Disconnecting the 3-wire connector by pulling it straight up. Remember the direction of the white connector. (Fig. IV in the following picture)

7. Loosen and remove the two white screws (Fig. I and II in the following picture) from the deuterium flange. Then pull the Deuterium lamp out by pinching its flange. (Note: Fig. III is a positioning bolt, there’s no need to loosen it)

Zoom in of D2 Fig.4-3

IV

III

II

I

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8. Replace a new pre-aligned lamp provided by or an authorized Service Provider. Be sure the socket of the lamp flange matches with the positioning bolt. Then tighten the two screws.

9. Reconnect the wire connector (make sure the wire connection orientation is the same as step 6 .)

10. Turn on the instrument. After self-testing, select 300 nm and press【ZERO】. Check to make sure that the light beam is focused on the entrance slit of the monochromator. Since the lamp is pre-aligned, there will be minimum, if any, adjustment required.

11. Switch the instrument off, re-cover the lamp chamber and tighten the two screws. 12. Reinstall the instrument cover by positioning the front of the cover first and then sliding

the back of the cover over the backside grill plate. Be sure to prevent all wires from being pinched in the process.

13. Reinstall the four screws and the Cuvette Holder Control Knob . 2. Battery Replacement UV11 and 12 Series can save many data in the RAM, so a battery must be used in the mainboard. When the battery is disabled, you’ll have to replace it as the following indication.

1) Turn off and unplug the instrument (VERY IMPORTANT: HIGH VOLTAGE ). 2) Unscrew the 13 white screws from the bottom board and remove the board.

(Fig.4-4)

Fig.4-4 Bottom

3) Pick out the old battery and replace a new one. (Fig.4-6)

Fig. 4-5 Main Board 4) Recover the bottom board and tighten the 13 screws.

Battery

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Troubleshooting

PROBLEM Possible Cause Solution

Power cord not connected to outlet Plug instrument in Dead Power outlet Change to a different outlet Wrong voltage setting Switch to your local voltage or

call an authorized service engineer

Instrument Inoperative after switch power on

Internal fuse blown or defective electronic component

Call an authorized service engineer

Light beam blocked: Holder misaligned

Check sample holder

Lamp is old or defective Replace lamp Lamp is off alignment Refer to lamp replacement

instructions in this manual

Instrument cannot set 100%T (0.000A)

Defective electronic component Call an authorized service engineer

No sufficient warm up time warm up at least 20 minutes Use glass cuvette in Ultra-violet range

Change to quartz cuvette

Significant temperature change Lamp not adjusted properly

Check lamp if has been properly installed. Refer to lamp replacement instructions in this manual

Lamp old or defective Replace with a new lamp

Unstable power supply equip a manostat to make the voltage stable

Instrument drift and noise

Defective or dirty detector or defective electronic component


Insufficient sample volume Fill cuvette with more samples

Wrong wavelength setting Failed to blank (0A/100%T)

Check analytical procedure and wavelength setting. Get dark current and calibrate wavelength in utility menu by press【SET】.

Stray sample preparation vapors. Prepare sample away from instrument. Use proper ventilation

Bubbles or particles in solution Check sample preparation and analytical procedure

Incorrect readings obtained

Instrument out of electronic calibration


instruction manual for spectro-uv11/v12/uv12

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