injectable alginate/poly(γ- glutamic acid)/f127 gel for bone formation and differentiation
TRANSCRIPT
Injectable alginate/poly(γ-glutamic acid)/f127 gel for bone formatio
n and differentiation.
• Cellulose (CELLU) ( rejected )
• Textile Research Journal - Manuscript ID TRJ-14-0115 ( rejected )
• eXPRESS Polymer Letters (EPL-0005595) ( rejected )
• Preparation of gel samples
• Swelling ratios test
• Water retention capacity test
• Tensile tests
• Evaluation of platelet adhesion
• Blood coagulation test
• Cell culture and MTT assay
Table 1. The preparation and the compositions of hydrogel blends
Ingredient Designation
Aalginate
CaCl2 A-C
Na-PGA(with CaCl2) A-NP
Ca-PGA
0% F127 A-CP
5% F127 A-CP-5F
10% F127 A-CP-10F
15% F127 A-CP-15F
20% F127 A-CP-20F
25% F127 A-CP-25F
30% F127 A-CP-30F
Figure 1 The swelling ratio of
A-C, A-NP, A-CP, and A-CP-F series hydrogels with nor
mal saline at different pH.
0
0.5
1
1.5
2
A-C A-NP A-CP A-CP-
5F
A-CP-
10F
A-CP-
15F
A-CP-
20F
A-CP-
25F
A-CP-
30F
swel
ling
ratio
s
pH4 pH7 pH10
Figure 2 The swelling ratio of A-C, A-NP, A-CP, and A-CP-F series
hydrogels in DI water at different temperature.
0
1.5
3
4.5
A-C A-NP A-CP A-CP-
5F
A-CP-
10F
A-CP-
15F
A-CP-
20F
A-CP-
25F
A-CP-
30F
swel
ling
ratio
s
50°C
37°C
30°C
25°C
Figure 3 The water retention of A-C, A-NP, A-CP, and A-CP-F series
hydrogels with the increase of
centrifugal force.
0
50
100
A-C A-NP A-CP A-CP-5F
A-CP-10F
A-CP-15F
A-CP-20F
A-CP-25F
A-CP-30F
WR%
Figure 4 Tensile strength and breaking elongation of A-
CP-F series hydrogel.
0
0.15
0.3
0.45
0 5 10 15 20 25 30
F127 content (%)
Tens
ile S
treng
th (M
Pa)
0
25
50
75
Tensile Strength
Breaking Elongation
Bre
akin
g El
onga
tion
(%)
Figure 5 The dependence of the adsorption amount of HSA and HPF on A-C, A-NP, A-CP, and A-CP-F series hydrogel.
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
0.5
HPF HSA
prot
ein
adso
rptio
n (μ
g/cm
2 )
A-C
A-NP
A-CP
A-CP-5F
A-CP-10F
A-CP-15F
A-CP-20F
A-CP-25F
A-CP-30F
Figure 6 The platelet adhesion of A-C, A-NP, A-CP, and A-CP-F series hydrogels.
0
25
50
75
100
30 90Time (min.)
Plat
elet
Adh
esio
n (%
)
A-C A-NP A-CP A-CP-5F A-CP-10F
A-CP-15F A-CP-20F A-CP-25F A-CP-30F
Figure 7 Comparison of anticoagulati
on time of A-C, A-NP, A-CP, and
A-CP-F series hydrogels.
0
15
30
45
APTT PT
Tim
e (s
ec.)
Control
A-C
A-NP
A-CP
A-CP-5F
A-CP-10F
A-CP-15F
A-CP-20F
A-CP-25F
A-CP-30F
Figure 8 MTT assays of A-C, A-NP, A-CP,
and A-CP-F series
hydrogels with MG63 cel
l.
0
0.15
0.3
0.45
3days 7days 14days 21days
ELIS
A a
bs
Control
A-C
A-NP
A-CP
A-CP-5F
A-CP-10F
A-CP-15F
A-CP-20F
A-CP-25F
A-CP-30F