Antigen antibodi 3

Antigen antibodi 3Dr.dr. I Wayan Putu Sutirta Yasa, M.SiGambar Berbagai Antigen dan EpitopI Wayan Putu Sutirta Yasa

Repeating Antigenic Determinantse.g. PEPTIDOGYCANMovement at the HingeRegionCH2 CH3CH2 CH3IgGSurface of an Antigeni.e. bacterialcell surface

I Wayan Putu Sutirta Yasa Gambar Konyugat Hapten-Karier merupakan Imunogen dan Hapten merupakan Antigen yang tidak selalu imunogenEpitopes: Antigen Regions that Interact with AntibodiesI Wayan Putu Sutirta Yasa

Antibodies are Proteins that Recognize Specific AntigensI Wayan Putu Sutirta Yasa

I Wayan Putu Sutirta Yasa Gambar Ikatan silang (cross-linkage) antara TCR dan molekul MHC-II8Clonal Selection of B Cells is Caused by Antigenic StimulationI Wayan Putu Sutirta Yasa

I Wayan Putu Sutirta Yasa

Gambar Perkembangan kadaar Ig serum pada manusiaI Wayan Putu Sutirta Yasa

Gambar Respons Antibodi Primer dan SekunderI Wayan Putu Sutirta Yasa

I Wayan Putu Sutirta Yasa

Gambar Valensi dn Aviditas interaksi antigen-antibodiI Wayan Putu Sutirta Yasa

Gambar Pembentukan kompelks imun dan presipitasiI Wayan Putu Sutirta Yasa Fungsi AntibodiI Wayan Putu Sutirta Yasa

Laboratory on immunoassayThere are two major type measured :Non Specific measured : hematology, biochemistry, urinary test Specific measured : serology test may be developed a number of its own techniques, particularly those based on the antigen-antibody interaction17Access is anImmunoassay System18Cardiac MarkersChemical (Clinical Chemistry)10-1210-910-610-310-0mg/mLg/mLpg/mLng/mLmg/mLThyroid HormonesCancer MarkersVitaminsImmunological (Immunoassay)Therapeutic DrugsFertility HormonesAllergy MarkersInfectious Disease18Basic concepts Based on : Ab + Ag = Ab:Ag (are not visualized)Ab:Ag (in proportions at or near equivalence, can be seen by colour (enzyme, fluorecence, protein stain); solid phase (ery, bentonite, latex); X ray (a,b,g : detect by machinary counter), as marker.In vivo similar to in vitroVan der Waals Energy : specificity of Ag & Ab Columbic Energy : electricityDistance between antigen and antibody pH, hydrogen, hydrophobic, electrostatic, etc19Immunoassay methodsDivided three major methods:Precipitation methods : high concentration, in solution: immunodiffusion; radial immunodiffusion, immunoelectrophoresisAgglutination methods: Ag / Immunoglobulin on solid phased : slide / tube ; agglutination inhibition; incomplete antibody tests ; complement fixation Amplification methods: low concentration( methods ; ELISA, RIA, PCR, IMUNOFLURESENSI) immunofluorescense ; ELISA ; EIA; radioimmunoassay; flow cytometry

20TERIMA KASIH

I Wayan Putu Sutirta Yasa