improvements in swimbladder inflation in yellowtail kingfish (seriola lalandi) larvae lindsey...
TRANSCRIPT
![Page 1: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/1.jpg)
Improvements in swimbladder Improvements in swimbladder inflation in yellowtail kingfish inflation in yellowtail kingfish
((Seriola lalandiSeriola lalandi) larvae) larvae
Lindsey WoolleyFlinders University
Supervisors:A/Prof. Jian QinDr Bennan Chen
Wayne Hutchinson
![Page 2: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/2.jpg)
Problem
Cleans Seas Tuna production > 1 million YTK fingerlings per year
Currently ) 10 % larval survival rates
High swimbladder malformations per production run 0-60 days post hatch (dph)
Failed inflation = Decreased survivability in larval rearing
![Page 3: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/3.jpg)
Introduction Swimbladder internal gas-filled sac, contributes to
the ability of a fish to maintain neutral buoyancy
Flexible-walled organ found dorsally below the notochord
Impermeable to gas: poorly vascularized and lined with a sheet of guanine crystals
Swimbladder of a rudd (Scardinius erythrophthalmus)
![Page 4: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/4.jpg)
7 dph larvae without swimbladder inflation
Failed initial inflation
Linked to abiotic factors
Abnormal development, liquid dilated swimbladder collapses with hypertrophy of epithelium
Swimbladder malformationSwimbladder malformation
![Page 5: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/5.jpg)
Why is swimbladder malformation Why is swimbladder malformation detrimental?detrimental? Decreased survival
higher mortality under stress
Delayed growth fish with no functioning swimbladder are 20-30% smaller in weight
than normal fry
Skeletal deformities occurrence of lordosis (curvature of the 2nd and 3rd vertebrae)
Metabolic demands higher abnormal larvae have buoyancy abnormalities higher energy requirements to maintain normal swimming
behaviour
= reduced production performance
![Page 6: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/6.jpg)
Project objectives
Increase swimbladder inflation rates of YTK larvae (< 2 % malformation)
Determine body density and distribution of larvae in rearing tanks
Determine abiotic factors that promote optimum swimbladder inflation
Increase overall survival rates to 25 % by 2011
![Page 7: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/7.jpg)
Research plan
Develop a standardized protocol to assess swimbladder inflation use of anaesthetics compromises swimbladder
volume
Describe larval swimbladder development morphology and histological assessments
(0 - 10 dph)
Determine effect of swimbladder inflation on body density and larval distribution within rearing tanks density and distribution studies
1. Swimbladder and body density assessment
![Page 8: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/8.jpg)
Research plan2. Abiotic factors
Investigate effects of surface skimmers Skimmers remove oil from water surface Allow larvae to gulp air at the surface for
initial inflation
Photoperiod Natural vs. artificial (halogen) light Various photoperiod light regimes
Temperature 20 – 25 °C
![Page 9: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/9.jpg)
Research plan3. Commercial validation
Assess YTK swimbladder malformation in weaned larvae CST Arno Bay Hatchery 40 dph YTK
Determine consequences of YTK swimbladder malformation on grow-out CST Arno Bay sea cages Fingerling – juvenile (5 g - 500 g)
Validate findings with Southern bluefin tuna (SBT, Thunnus maccoyii) larvae Describe swimbladder development in SBT Investigate effect of abiotic factors on
swimbladder inflation rates
![Page 10: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/10.jpg)
0 – 2 days post hatch
Forms as an envagination of the digestive tract
Lumen increases in size
Epithelium cells decrease in thickness to allow for dilation
Rete mirabile develops as fine capillaries
Lumen is liquid-filled at this stage
ResultsYTK swimbladder development
![Page 11: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/11.jpg)
3 – 5 days post hatch
Inflation occurs within a discrete window between 3 – 5 dph
Liquid- filled bladder becomes inflated with air … ? process is still not understood properly
Gas gland develops at the anterior pole, composed of squamous epithelium
Rete mirabile increases in complexity – functions as a countercurrent exchanger
Critical period for Critical period for swimbladder inflationswimbladder inflation
![Page 12: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/12.jpg)
Post larval stage Reared in hatchery (0 -40 dph)
3.5 g
Reared in nursery (40 – 60 dph) 3.5-5 g
Grow-out in sea cages (>5 g)
7 dph larvae with an inflated swimbladder9 dph larvae with
collapsed swimbladder
8 dph larvae with inflated swimbladder
![Page 13: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/13.jpg)
Fish with non-inflated swimbladdersFish with inflated swimbladders
Specific gravity With inflation: 1.022 g cm-3
Without inflation: 1.030 g cm-3
MANOVA: P< 0.001
2 dph liquid-filled
Swimbladder
Initial Inflation
Body density change with growth
Inflation failure = sinking death syndrome (10 – 15 dph)
An increase in bladder volume = larvae are less dense then their environment and rise to the surface
![Page 14: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/14.jpg)
Commercial applicationsCommercial applications
Increase the rate of swimbladder inflation, contributing to the overall increase in larval survival rates
Define a standard protocol for swimbladder assessment at 3 – 5 dph larvae
Define the range of abiotic factors that promote optimal swimbladder inflation
Compare the swimbladder development between YTK and SBT
Increase production of YTK larvae to a 25 % survival rate by 2011
![Page 15: Improvements in swimbladder inflation in yellowtail kingfish (Seriola lalandi) larvae Lindsey Woolley Flinders University Supervisors: A/Prof. Jian Qin](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56649cab5503460f9496bc8c/html5/thumbnails/15.jpg)
Acknowledgements Australian Seafood Cooperative Research Centre“This work formed part of a project of
the Australian Seafood Cooperative Research Centre, and received funds from the Australian Government’s CRCs Programme, the Fisheries R&D Corporation and other CRC Participants”.
Flinders University • A/Prof. Jian Qin
SARDI-Aquatic Sciences • Dr Bennan Chen • Wayne Hutchinson
Clean Sea Tuna Ltd. Arno Bay Hatchery • Mike Thomson • Alex Czypionka
Hatchery system images courtesy of Paul Skordas
(SARDI Hatchery)