improved analytical technologies for detection of foodborne ......cyclodextrin, polyurethane...
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Improved Analytical Technologies for Detection of Foodborne Toxins and
Their MetabolitesCRIS 5010-42000-049-00D
Chris Maragos
Mark Busman Mike Appell
Zakir Hossain
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Monitoring: the first (and last)line of defense
• Supports efforts to mitigate toxin production• Supports efforts to determine toxicological relevance
– Hazard assessment– Trace-back causation
• Reduces exposure– Improves food safety– Improves feed safety
• Facilitates trade
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Goals of this CRIS
• Improve Methods for Detection of Toxins & Related Metabolites
• Reduce Exposure to Toxins& Related Metabolites
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Objectives• Improved Detection Methods
Objective 1: Improve detection of foodborne toxins through development of novel technologies based upon biosensor platforms and new component materials.
Objective 2: Improve detection of foodborne toxins through development of direct detection technologies based upon novel mass-spectrometric platforms
Objective 3: Improve the ability to detect and measure “masked” mycotoxins and biomarkers of mycotoxin exposure in commodities and foods
• Reduce ExposureObjective 4: Improve toxin detection methods and reduce exposure through the development and application of synthetic materials.
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Recent Accomplishments(mile high view)
• Objective 1 (Biosensors)• iSPR biosensor for T-2 toxin,
Zearalenone (ZEA) & Deoxynivalenol(DON) in maize
• Biolayer Interferometry Sensor forDON in wheat dust (Sanders)
• Antibodies & immunoassays forCyclopiazonic acid (CPA) in maize & cheese
• Rare Earth Luminescence assay forCPA
• Objective 2 (Novel Mass Spec Methods)• Laser-Assisted
Ambient ionization-mass spectrometryaflatoxin B1
O
O
OHOH
O OH
HH
HO
OH
O
O
O
NH
N
O
O
O
H
H
H
OH3C
O
OCH3
H3COH
O
H
H3C O
CH3
O
H
O
H3C O
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Recent Accomplishments(mile high view) (2)
• Objective 3 (Masked Mycotoxins)• Antibodies & Immunoassays for
masked fumonisins• LC-HRMS for masked fumonisins
• Objective 4 (Reducing Exposure)• Molecularly imprinted polymers (MIPs)
for citrinin• “Nanosponges” for ochratoxin A (OTA)• Computational modeling of zearalenone
(ZEN), CIT, & trichothecenes
N
C
O
HO
O
O
O
CH3
H
Cl
OH
H
H
HN
OHOH
OHO
OHO O
O
HO
O
OHO O
O
HO R
O
OH
C
O
HO
O
CH3
CH3
H CH3H
HO
OH
O
O
O
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Objective 1:Novel Biosensors
Md Zakir Hossain
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Imaging Surface Plasmon Resonance (iSPR)
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iSPR Biosensors• Multiplexing: one sample extract, one chip,
multiple tests
• Reusable chips
• Fairly rapid for quantification
• Can be made more rapid for qualitative testing
• Significant potential for automation
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Multiplex Biosensor for Fusarium toxins in wheat
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Immunoassays for CPA
NH
N
O
O
OH
H
H
H
CPAA neurotoxin
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Luminescent Probes for CPA
(1) Detection of CPA(2) Mechanism of
Action
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Objective 2:
Novel Mass Spectrometric Methods
Ambient ionization MS of mycotoxins
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Presentation of Small Surfaces to the DART-MS Interface
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Advantages of Laser Assistance to Dart-MS
– allows analysis of difficult analytes– allows spatial resolution of sampling– applicable to imaging applications
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Accomplishments for ambient ionization objective (laser assisted DART-MS)
– construction and characterization of IR laser assisted DART-MS apparatus
– development of technique for IR laser assisted DART-MS analysis of AFB1 in maize extract
– development of technique for IR laser assisted DART-MS analysis of AFB1 from glass slides
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Accomplishments for ambient ionization objective (multiplexed analysis)
-development of technique for DART-MS multiplexed analysis of AFB1 and T-2 toxin from maize extract-DART-MS and other ambient ionization methods compared for multiplexed analysis of 10 common Fusarium toxins in maize extract-development of technique for paper spray-MS analysis of fumonisins
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Objective 3
Detection of “Masked” Mycotoxins
Antibodies/Immunoassays/Biosensors
Mass Spec-Based Methods
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Conjugated form (masked)(hidden from traditional monitoring techniques)
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Antibodies for NDFrc-FB1
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Application of Immunoaffinity columns and LC-MS for detection of masked fumonisins
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Isotope ratio calibration curve: matrix standards
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Naturally contaminated maize
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Example analyses:Type FB1 (ppm) FB1-frc (ppm)
Corn meal 0.22 (0.17) 0.54 (0.47)
Maize (artificially contaminated) 200.14 (32.06) 21.68 (2.02)
Maize (naturally contaminated) 10.26 (1.67) 3.37 (0.40)
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Synthetic Materials for Detection of Citrinin
p-quinone o-quinone
Citrinin is a toxic contaminant of a number of agricultural commodities, including corn, grains, cheeses, and notably Monascus-fermented red rice
Associated with kidney and liver damage, and often occurs with aflatoxins and ochratoxins
EU has set a regulated limit of 2000 ppb for citrinin in Monascus-fermented rice products (supplements)
Advisory limits for exposure in agricultural commodities have been issued for Japan (200 ppb) and EU (100 ppb)
Developed an analytical method to improve determination of citrinin in corn using a novel synthetic material for sample clean-up
Why synthetic materials? economical capability to incorporate chemical groups that do not exists in biological
binding materials solvent-tunable affinity that can overcome the limitations of current
materials
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Preparation of Molecularly Imprinted Polymers (MIPs) for Citrinin
Rational design of materials
MISPE Analysis SUMMARY•Deprotonation of citrinin is a major factor governing the shifts in fluorescence excitation maxima.•A reliable method to detect citrinin in maize was developed using LC-fluorescence detection with MISPE sample clean up (LOD 0.01 µg g-1 ; LOQ 0.03 µg g-1 ).•Significant recoveries of citrinin were obtained (82.3-91.5%) between 0.03 - 3 µg g-1
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Preparation of “Nanosponges” for Sequestration of Ochratoxin A
Ochratoxin A can contaminate a variety of commodities and foods, including fruit juices and wines.
SPERP-HPLC/FLD
Nanoscale features enable the novel β-cyclodextrin, polyurethane nanosponge material to selectively bind ochratoxin A in grape juice and wine during SPE sample clean-up.
A liquid chromatography method using fluorescence detection (LC-FLD) was capable of detecting ochratoxin A levels below regulatory levels in grape juice and wine.
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Use of Cheminformatics and Bioinformatics to Inform Detection Properties
In collaboration with Prof. Yufeng Jane Tseng’s group at National Taiwan Universityand Dr. Yi-shu Tu, a visiting scientist at NCAUR
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Predictive Modeling of Cross-Reactivity Based Upon Structural Features
Trichothecenes
very large family of chemically related mycotoxins
False-positive detection is associated with spatial autocorrelation indices.Trichothecenes with bond connectivity similar to DON can interfere with DON detection
Zearalenoneand analogs
potent estrogenic metabolite produced by some Fusarium and Gibberella species
OH
HO
O
O
O
pH and the general structure of zearalenone must be conserved during fluorescence analysis to ensure accurate quantitation
AlternariolAlternariol monomethyl etherEuropean Commission has mandated the development of standardized methods for Alternaria toxins in foods
Quantum chemical analysis indicatesUV and Fluorescence based detection require separation techniques to distinguish between alternariol and alternariol monomethyl ether
Future Projects• More accurate determination of Ochratoxin A in oats• Sample clean-up through green imprinted magnetic beads • Incorporation of synthetic materials into additive manufacturing
at NCAUR
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Accomplishments (Non-Publication)
• MTAs – antibodies (old mechanism) • MTRAs• CRADA -Biosentinel• Biological Material Commercial Evaluation
Agreements – antibody producing cell lines• Licensing Agreements ~30• Testing kits based on these materials are
provided by several manufacturers and are sold world-wide
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Thanks for your attention!