importance of genetics.ppt
TRANSCRIPT
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USEFULNESSOF GENETICS
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• A.GENETICS & PROMOTION
OF GOOD HEALTH
• B. GENETICS & THE USE OFDNA TECHNOLOGY
• C. GENETICS &
IMPROVEMENT OFLIVELIHOOD
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GENETIC
ENGINEERING• genetic modification,
• is the direct human manipulation
of an organism's genome usingmodern DNA technology.
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DNA TECHNOLOGY
• Wide range of techniques used
to study , improve, create or
manipulate the genetic material
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DNA TECHNOLOGY
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•Gives rise to biochemical
products
• vaccines, hormones, enzymes ,
•genetically recombined
microbes, plants & animals;
•
medicines for gene therapy &•methods for genomic analysis
(DNA fingerprinting)
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A.GENETICS &
PROMOTION OF GOOD
HEALTH• GMO/GEO
– Genetically Modified Organism
– Genetically Engineered Organism – Organisms whose genetic material has
been altered using genetic engineering
techniques
– Use in biological & medical research,production of pharmaceutical drugs ,
experimental medicine(gene therapy),
agriculture (golden rice)
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TECHNIQUES IN DNA
TECHNOLOGY
• 2 CATEGORIES:
– 1. RECOMBINANT DNA
TECHNOLOGY – 2. DNA TECHNOLOGY WITHOUT
RECOMBINANT DNA
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RECOMBINANT DNA
TECHNOLOGY
• Refers to a process of creating a
DNA molecule by joining together
DNA or segments of DNA from 2diff. species
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STEPS in Recombinant
DNA Technology
• 1. 2 kinds of
DNA molecule
–
1 gene of interest
(eukaryotic cell)
– Other , bacterial
plasmid
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STEPS in Recombinant
DNA Technology
2. The 2 DNA are
cut at a site
with specific
sequence=
RESTRICTION
ENZYME
=fragment have
the same
recognition
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Cutting DNA•
Restriction enzymes cutDNA at specific sequences
• Useful to divide DNA into
manageable fragments
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STEPS in Recombinant
DNA Technology
3. Restriction
Fragments from 2
diff. cells have the
same recognitionsequence=join as
complementary
strands
=RECOMBINANT
DNA
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STEPS in Recombinant
DNA Technology
4. Recombinant
DNA=allowed to
produce in the
bacterial cells
-several copies of
gene
GENE CLONING=
process by whichmultiple copies of
the desired gene
are produced.
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DNA TECHNOLOGY
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DNA TECHNOLOGY
WITHOUT
RECOMBINANT DNA• Do not require the use or
creation of recombinant DNA
• Embark on a search for a certaingene
• Isolated , identified , sequenced
& amplified
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Copying DNA•
Polymerase Chain Reaction • Also called PCR
• A method of making many
copies of a piece of DNA
B GENETICS & THE
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B. GENETICS & THE
USE OF DNA
TECHNOLOGY• Diff. Technologies associated
with genetics as a science
– Includes:• RECOMBINANT DNA TECHNOLOGY
• CLONING
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Cloning
• Clone -
a memberof a group ofgenetically
identical cells• May be produced
by asexual
reproduction (mitosis)
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Cloning organisms
• A body cell from oneorganism and an egg cell
from another are fused• The resulting cell divideslike a normal embryo
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Cloning “Dolly”
B GENETICS & THE
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B. GENETICS & THE
USE OF DNA
TECHNOLOGY• Diff. Technologies associated
with genetics as a science
– Includes:• RECOMBINANT DNS TECHNOLOGY
• CLONING
• GENETIC PROFILING
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DNA PROFILING
• DNA profiles
• are encrypted sets of numbers that
reflect a person's DNA makeup• used as the person's identifier-
criminal investigations
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C GENETICS &
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C. GENETICS &
IMPROVEMENT OF
LIVELIHOOD• Contribution in the improvement
of crops & welfare of domestic
animals=selective breedingtechniques
• Improve quality of food, make us
healthier to have longer life span
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Benefits of GeneticEngineering
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Biotechnology - • The use of gene
science to create newproducts from plantsand animals
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Biotechnology
Provides:• Improved food products
• Medical advances
• An enhanced environment
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Biotechnology Breakthroughs
• Insulin (1982)– First commercial biotechproduct
–
Reliable, inexpensive source ofinsulin
• Rice– Enriched with beta-caroteneand iron
• Bananas– Containing edible hepatitis
vaccine
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Biotechnology Breakthroughs
• Potatoes with higher solidcontent
• Garlic that lowers cholesterol
• Fruits and vegetables that
reduce risks of cancer andheart disease
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Environmental Benefits
• Reduced pesticide use
•
Lower energy requirements• Cleaner water
•
Less soil erosion