immunosuppressive diseases in commercial chickens
TRANSCRIPT
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Immunosuppressive Diseases in Commercial
Chickens
• Frederic J. Hoerr, DVM, PhD
• Joint Pathology Center
• October 30, 2019
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Bursa of Fabricius and B lymphocytes• The embryonic bursa is populated by
precursor embryonic cells from yolk sac and liver
• B Lymphocytes develop in medulla and migrate to cortex
• Most are destroyed by MHC selection• Migrate to blood stream and populate
secondary tissues• 12,000 follicles, each follicle has 2-500,000
cells
Sci Rep. 2018; 8: 16905. Published online 2018 Nov 15. doi: 10.1038/s41598-018-34897-4; Kwang Hyun Ko, et al.
Fellah et al, Avian Immunology 2nd, 2014, 45-63
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Thymus
• T-cells mature while traversing from cortex to medulla
• 95% of developing T lymphocytes are destroyed in development through Major Histocompatability Complex selection
• Mature T lymphocytes depart through venules at cortico-medullary junction
• Distribute to secondary lymph organs: spleen, Peyer’s patches, cecal tonsil
• Delicate balance of mature T Lymphocyte production, development, and supply to secondary organs – easily disrupted
http://bszm.elte.hu/anatomy/birds/16/
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SpleenPeriarteriolar lymphoid sheath (PALS), rich in T lymphocytes that are seeded by the thymus
Germinal centers composed of B Lymphocytes, seeded by the bursa, and plasma cells
Ellipsoid containing lymphocytes and reticular cells
Red pulp
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Recognizing Immunosuppression
• Poor/harsh vaccination responses• Exaggerated morbidity/mortality
• Early stress, later problems• Viral enteritis - Runting stunting syndrome
• Early exposure to immunosuppressive diseases• Bursal disease• Chicken infectious anemia• Marek’s disease• Newcastle• Reovirus (Viral Arthritis), Reticuloendotheliosis, ALV-J
• Diseases associated with immunosuppression• Inclusion body hepatitis, respiratory disease,
coccidiosis, gangrenous dermatitis
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Infectious Bursal Disease –Classical and Variant
Delaware Variant
R. W. Winterfield
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Very Virulent Bursal Disease (vvIBD)
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Chicken Infectious Anemia
Crowther et al, J Virol. 2003 77: 13036–13041 AAAP Slide Set
Blue WingAla Azul
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Marek’s DiseaseImmunosuppression Skin lymphoma and tumors
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Marek’s DiseaseImmunosuppression
Marek’s Disease Control
• Occurs at a young age• Difficult to confirm • Assume early immunosuppression if
Marek’s tumors or clinical disease are present
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Broiler Viral Enteritis“Runting Stunting Syndrome”
06.1029
Bursa Atrophy
Thymus Atrophy
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Broilers: Intestinal Virus Detection by Age
1 7 14 21 28 35 420
5
10
15
20
25
AstrovirusRotavirusReovirus
N=258 Cases
Age - Days
No.
Cas
es
Broilers: Cases by EntericViruses Detected
Astrovir
us
Rotaviru
s
Reovir
us
AstroRota
AstroReo
RotaReo
AstroRotaR
eo0
20
40
60
80
N=258 Cases
Virus
No.
Cas
es
Viral enteritis diagnosis: Retrospective PCR and Immune System Histopathology
Virus Positive Broilers <20 days:Maximum Thymus Depletion Score
RSS
Astrovir
us
Rotaviru
s
Reovir
us
AstroRota
AstroReo
RotaReo
AstroRotaR
eo0
1
2
3
4
Normal
N=131 Cases
Virus
Max
Thy
mus
Sco
re (M
ean)
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Day JM, Oakley BB, Seal BS, Zsak L (2015) Comparative Analysis of the Intestinal Bacterial and RNA Viral Communities from Sentinel Birds Placed on Selected Broiler Chicken Farms. PLoS ONE 10(1): e0117210. doi:10.1371/journal. pone.0117210
Enteric Virome Metagenomic Analysis: SPF Sentinals on 6 broiler farms
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Experimental Mycotoxicosis:Target Organs
Toxin Liver Kidney Immune Skin/Oral Other
AflatoxinSterigmatocystinCyclopiazonic acid
+++ + + +
TrichothecenesFusarochromanone
Moniliformin
+ +++ +++ ++
Ochratoxin ++ +++ + +Citrinin +++
Oosporein +++
Ergot +++ ++
DAS -Diacetoxyscirpenol
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Mycotoxin ImmunosuppressionToxin Lymphoid
AtrophyCell
MediatedHumoral Disease
InteractionTrichothecenes(T-2. DAS, Others) + + + +Moniliformin + +Fumonisins + +Aflatoxin + + + +Ochratoxin + + + +Citrinin +Cyclopiazonic Acid +Sterigmatocystin +
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Broiler, 50 days, hepatosis
• Bile duct proliferation• Differential diagnosis
• Hepatotoxic mycotoxins• Aflatoxin, cyclopiazonic acid,
sterigmatocystin• Implications
• Damaged liver• Altered hepatic processing of
nutrients• Immunosuppression
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Variant Reovirus -Challenge Study
VETERINARY DIAGNOSTIC PATHOLOGY, LLC 17
HeartTendon
Bursa follicular lymphoid depletion
Thymus cortex lymphoid depletion
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Newcastle Disease - Lymphocyte Necrosis and Depletion
Spleen ThymusCecal tonsil
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Stages of Bursal Disease: Histopathology• Normal Bursa
• Virus infection, acute necrotizing phase• Lymphocyte necrosis/apoptosis –rapid progression• Strain variable
• Apoptosis and lymphoid depletion, absent or minimal inflammation
• Hemorrhage, edema, granulocytic and histiocytic inflammation• Cytotoxic T cells rapidly enter and associated with decline of viral load• End point: the lymphocyte population is destroyed
• Follicular restitution phase• Absent to substantially complete• Small undifferentiated follicles, lower immunocompetence• Large differentiated follicles, higher immunocompetence
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21
1A 2A 3A 5A
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Stages of Infectious Bursal Disease
Normal
Score 0Diffuse necrosis (Score 5, acute)
5
4
3 2 1
Recovery Phase
Follicular Restitution
5 Acute
Acute Phase
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Post Bursal Disease Follicular RestorationSmall vs. Large Follicles • Small undifferentiated
follicles• Lymphocyte restoration
from medullary stem cells• Minimal or no immune
response capability●Large follicles
– Rapidly proliferating B cells– Correlated with partial recovery of
antibody response– Withers et al. Immunology 117, 558-565,
2006– The antibody response does not occur
in the bursa– M. E. Ifrah et al. Poultry Science 96, 2017
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Age of Immune InjuryBursa and Thymus: Critical First 14 DaysPrimary lymphoid organs seed secondary organs
B Lymphocytes
Cecal Tonsil/GutSpleen Gland of Harder
T Lymphocytes
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Bursa Survey: 6 flocks – Spleen Germinal Centers (B-cell centers)
Age Units B1 B2 B3 B4 B5 B6 MeanVirus
Detect #Nec Comment
14 days 1 1 1 1 1 1 1 0 Within normal limits
21 days Tissue not available
29 days 3.5 3.5 3.5 3.5 2 2 3 2 0 Recovery IBD
34 days 3 3 3 3.5 3 1 2.75 2 0 Recovery IBD
41 days 3 3 3 3 3 1 2.6667 2 0 Recovery IBD
14 days 1 1 1 1 1 1 Within normal limits
21 days 1 1 1 1 1 1 Within normal limits
28 days 4 4 1 4 4 3.4 1 4 Acute bursitis/IBD
33 days 3.5 3.5 3.5 3 3.5 3.4 2 0 Recovery IBD
41 days 3 3 3 3 3 3 2 0 Recovery IBD
41 days 3 3 3 3 3 3 2 0 Recovery IBD
14 days 1 1 1 1 1 1 Within normal limits
21 days 4 4 3.5 1 1 2.7 1 3 Acute bursitis/IBD
28 days 4 3.5 4 3 3 3.5 2 0 Recovery IBD
33 days 3 3 3 3 3 3 2 0 Recovery IBD
41 days 2.5 3 3 3.5 3 3 2 0 Recovery IBD
14 days 1 1 1 1 1 1 Within normal limits
22 days 1 1 1 1 1 1 Within normal limits
29 days 3 3 3.5 3.5 3.5 3.3 2 0 Recovery IBD
34 days 3 3 3.5 3 3 3.1 2 0 Recovery IBD
41 days 3 3 3 3 2.5 2.9 2 0 Recovery IBD
41 days 3 2.5 3 3 3 2.9 2 0 Recovery IBD
14 days 4 1 1 1 1 1.6 1 1 Acute bursitis/IBD
21 days 3 3.5 3.5 3.5 3.5 3.4 2 0 Recovery IBD
28 days 3.5 3.5 3.5 3.5 3.5 3.5 2 0 Recovery IBD
32 days 2.5 2.5 2.5 2.5 3 2.6 2 0 Recovery IBD
41 days 2.5 2.5 2 2 2.5 2.3 2 0 Recovery IBD
41 days 1 2.5 2.5 2.5 2.5 2.2 2 0 Recovery IBD
14 days 1 1 2 1 1 1.2 1 1 Acute bursitis/IBD
21 days 4 4 4 4 3.5 3.9 1 4 Acute bursitis/IBD
28 days 3 3 3 3 3 3 2 0 Recovery IBD
33 days 2.5 4 2.5 3 3 3 2 0 Recovery IBD
41 days 2.5 2.5 1 2.5 2.5 2.2 2 0 Recovery IBD
41 days 2.5 1 2.5 2.5 2.5 2.2 2 0 Recovery IBD
25.8
16.6
18.8
11.6
4.2
7.2
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Thymus Pathology: Lymphocyte Depletion
0=Within Normal Limits
5=Severe
2
3
4
0
5
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Immune Surveys by HistopathologyOctober 2018-October 2019
• Bursa: 1438 US Broiler flocks• Thymus & Spleen ~370 flocks
• Survey designs• 21 days (bursa only)• 12 - 24 days (mostly bursa, some thymus)
• ~2 day intervals• 12 – 45 day+, bursa, also thymus and spleen
• ~3 day intervals
• 21 states and territories
• Clinically normal chickens• 5-7 birds sampled per flock• 10-15 flocks per survey
• Pathologist blinded to vaccination program
• Data in Excel, pivot table analysis
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Bursa by Age and Lesions
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Bursa by Age and Bursa Score Trends
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Thymus by Age and Lesions
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Thymus by Age and Thymus Lymphocyte Depletion Trends
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Bursa and Thymus Lymphoid Depletion Trends
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Alabama Broilers 1990’s
Lymphocytic Depletion by Age ofSubmission (N=631)
10 15 20 25 30 35 40 45 50 55 60
1.0
1.5
2.0
2.5
3.0
3.5
4.0
Bursa ThymusAge at Submission to Laboratory (Days)
Mea
n De
plet
ion
Scor
e
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CAV Infection& Thymus Atrophy after Depleted Maternal Immunity
• No anemia observed• Lymphocyte and macrophage suppression
• T-cell growth factor, interferon, and transformation• Macrophage FC receptor expression, interleukin-1,
phagocytosis and bactericidal activity
CAV Challenge21 4935
Maternal antibody
Immunosuppression
Age-days
1
McConnell, Adair & McNulty (Avian Dis 37:366-374, 1993)
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Summary of 6-year Study: Alabama Broilers CAV infection was widespread Thymus atrophy associated with CAV infection CAV, thymus atrophy, and bursa atrophy were more prevalent in chickens
with clinical disease• Respiratory• Gangrenous Dermatitis• Coccidiosis
Hagood et al. Avian Dis 44:803-808. 2000.
Laboratory findings correlated with economic losses.
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Thymus and Bursa Lymphocyte Depletion
Bursa Lymphocyte Depletionby Maximum Thymus Depletion
Thymus 1 Thymus 2 Thymus 3 Thymus 41
2
3
4
Kruskal-WallisP<0.0001
aa,b
b,c c
Maximum Thymus LymphocyteDepletion
Mean
Bur
saLy
mpho
cyte
Deple
tion
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Spleen Germinal Center Trends
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Spleen Germinal Center Trends by Bursal Lesions
Normal Bursa
Acute Bursitis/IBD
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Bursa and Spleen Interaction 20-25 days
Mean GC Normal Bursa
Mean GC Acute IBD
Minimal GC Normal Bursa
Minimal GC Acute IBD
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Decreased Germinal
Centers at 20-25 days
• Reduction in antibody production in spleen and other GC-rich tissues
• Gut, conjunctiva, bronchi, biliary tract
• Gut is acquiring mature function• E. maxima peak scores at 26-27 days
• Respiratory vaccine virus shedding and reaction• Potential determinate of severity of reovirus
infection (tenosynovitis)
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Immunosuppressive Interactions in Broilers
Bronchitis, Influenza, Paramyxovirus, LT,
Pneumovirus
Bursal Disease
Chick Anemia
E. coli, Gangrenous Dermatitis, Coccidiosis
Marek’s, Viral enteritis
Mycotoxins
Other
Permanent Immunosuppression
Transient Immunosuppression
Cost, Livability
Feed Conversion
Condemnations
14 217 28 35 42
Hatch stress
Vaccination reaction
49
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[email protected] South Fort Valley RdFort Valley, VA 22652
CF.320.5.Veterinary Diagnostic Pathology, LLC 42
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Histopathology of Broiler Disease by Age
1 7 14 28 35 4221 49 56
Omphalitis, Aspergillus, Dehydration, Ammonia
Viral Enteritis
CAV Blue Wing
IBV/PMV Vx Reaction Harsh Immuno/cycling
Rickets
E. maxima
Necrotic enteritisCAV Thymus Atrophy/T-Cell
Bursal Dis / B-Cell
Bronchitis, PMV, LT Challenge
E. coli, Salmonella E. coli, Ornithobacterium, Enterobacter
Gangrenous Dermatitis/Cellulitis
Feed change
Feed manufacture and management, water, lights, ventilation, daily care
Coccidia Vx or challenge
Viral Proventriculitis
Adenovirus - vertical Adenovirus - immunosuppression
Ascites/Synovitis/Tendonosis/DPM