immunofluorescence atlas hep-2 plus
TRANSCRIPT
![Page 1: Immunofluorescence Atlas HEp-2 Plus](https://reader036.vdocuments.mx/reader036/viewer/2022071617/62d1bd21e1123879b50b90f0/html5/thumbnails/1.jpg)
Immunofluorescence Atlas HEp-2 Plus
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
International Consensus on Antinuclear Antibody Pattern (ICAP), 2015
http://www.anapatterns.org/
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
International Consensus on Antinuclear Antibody Pattern (ICAP), 2015
2018 new: http://www.anapatterns.org/
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: dsDNA, Histon, Nuclesome
Pattern: Interphase cells: homogenous staining of the nucleus Mitotic cells: chromatin area strongly positive
AC-1
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Pattern: Interphase cells: fine speckled staining of the nucleus Mitotic cells: chromatin area negative
Autoantigen: SS-A (Ro60 and/or Ro52)
AC-4
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigens: SS-B (La)
Pattern: Interphase cells: fine speckled staining of the nucleus Mitotic cells: chromatin area negative
AC-4
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigens: RNP, Sm
Pattern: Interphase cells: coarse speckled staining of the nucleus Mitotic cells: chromatin area negative
AC-5
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Pattern: Interphase cells: fine granular staining of the nucleus Mitotic cells: chromatin area negative
Autoantigen: Ku
AC-4
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: Mi2
Pattern: Interphase cells: very fine dense granular staining of the nucleus Mitotic cells: chromatin area negative
AC-4
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Pattern: Interphase cells: fine dense staining of the nucleus and staining of the nucleoli Mitotic cells: chromatin area positive with some dots
Autoantigen: Scl-70 (Topoisomerase I)
AC-29
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Pattern: Interphase cells: nucleolar clumpy in the nucleus Mitotic cells: reticular staining of chromatin area
Autoantigen: Fibrillarin
AC-9
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigens: Th/To
Pattern: Interphase cells: homogenous fluorescence of the nucleoli, weak granular staining of the nucleus possible Mitotic cells: chromatin area negative
AC-8
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Pattern: Interphase cells: staining of the nucleoli and weak granular staining of the nucleus Mitotic cells: chromatin area negative
Autoantigen: PMScl
AC-8
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigens: gp210 (nuclear pore)
Pattern: Interphase cells: fine granular staining of the nucleus with accentuation of the rim Mitotic cells: chromatin area negative
AC-12
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Pattern: Interphase cells: fine linear staining of the nucelar membrane and homogenous staining of the nucleus Mitotic cells: chromatin area negative
Autoantigens: Lamin (A, B1, B2 and C)
AC-11
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Pattern: Interphase cells: multiple dots (corresponds to number of chromosomes) in the nucleus Mitotic cells: Dots in chromatin area
Autoantigens: Centromere (CENP-A, -B, -C)
AC-3
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: sp100
Pattern: Interphase cells: multiple nuclear dots in the nucleus Mitotic cells: chromatin area negative
AC-6
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Pattern: Interphase cells: negative Mitotic cells: Centromere dots in prophase and metaphase, positive midbody in anaphase
Autoantigen: Centromere E
(AC-14)
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Pattern: Interphase cells: one or two dots in cytoplasm close to nucleus membrane Mitotic cells one dot each at the poles of mitotic spindle
Autoantigen: Centrioles
AC-24
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: mitotic chromosomes
Pattern: Interphase cells: negative Mitotic cells: exclusive positivity of mitotic chromatin from prophase to telophase
AC-28
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: MSA 2
Pattern: Interphase cells: granular nuclear fluorescence in G2 phase Mitotic cells: staining of centromeres in prophase and metaphase, staining of midbody in anaphase
AC-27
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: NUMA I
Pattern: Interphase cells: very fine dense staining of the nucleus Mitotic cells: chromatin area negative, staining of spindle poles and fibres
AC-26
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: NUMA II
Pattern: Interphase cells: negative Mitotic cells: chromatin area negative, staining of the spindle
AC-25
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: Ki-67
Pattern: Interphase cells: pleomorph staining of the nucleus Mitotic cells: chromatin area positive
(AC-13)
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: PCNA
Pattern: Interphase cells: pleomorph granular pattern of the nucleus, S-phase cells stained Mitotic cells: chromatin area negative
AC-13
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Pattern: Interphase cells: polar cytplasmic staining close to one side of the nucleus Mitotic cells: chromatin area negative
Autoantigens: Golgi
AC-22
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: AMA, Mitochondria type 2 (M2)
Pattern: Interphase cells: large cytoplasmic dots organized in a network of filaments Mitotic cells: chromatin area negative
AC-21
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Pattern: Interphase cells: diffuse staining of cytplasm, overlaying larger dots Mitotic cells: chromatin area negative
Autoantigen: Jo-1
AC-20
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: PL7
Pattern: Interphase cells: diffuse staining of cytplasm Mitotic cells: chromatin area negative
AC-19
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: PL12
Pattern: Interphase cells: diffuse staining of cytplasm Mitotic cells: chromatin area negative
AC-19
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: SRP
Pattern: Interphase cells: diffuse staining of cytplasm Mitotic cells: chromatin area negative
AC-19
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigens: Lysosomes
Pattern: Interphase cells: multiple small dots in the cytoplasm Mitotic cells: chromatin area negative
AC-18
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigen: GW Bodies
Pattern: Interphase cells: multiple large dots in the cytoplasm Mitotic cells: chromatin area negative
AC-18
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigens: ribosomal phosphoproteins and coilin (mixed pattern)
Pattern: ribP Interphase cells: very fine dense staining of the cytoplasm Mitotic cells: chromatin area negative Coilin Interphase cells: 2-5 dots in the nucleus Mitotic cells: chromatin area negative
AC-19, AC-7
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigens: Vimentine
Pattern: Interphase cells: fine filamentous staining of the cytoplasm Mitotic cells: covered with multiple dots
AC-16
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© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04
Autoantigens: Actin
Pattern: Interphase cells: rigid filamentous staining of the cytoplasm Mitotic cells: chromatin area negative
AC-15