ifocus_brochure_focus proteomics

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iFOCUS: A tablet-sized device for the IEF of proteins and peptides for clinical and basic research applications Why IEF? Immobilized pH gradients (IPGs) are stable and highly reproducible pH gradients for the isoelectric focusing (IEF) of proteins. IPGs are capable of separating protein charge isoforms differing by 0.001 pI units, a resolution an order of magnitude higher than labile carrier ampholyte generated pH gradients, which can resolve differences of only 0.01 pI units [1]. IPGs spanning 0.1 pH units have been described for the separation of proteins differing by a single amino acid substitution [2]. iFOCUS™ and SYNAPSE™ Technologies Conventional IEF devices are microprocessor controlled and utilize Peltier cooling systems, both of which significantly contribute to the cost of the instrument, but do not contribute to IEF performance. The iFOCUS is an IEF device with a footprint nearly identical to an Apple iPad. Elimination of unnecessary features such as Peltier cooling, microprocessors, and integrated power supplies dramatically reduces cost and makes the iFOCUS small size possible without compromising performance or capability. Versatile high performance IEF at low price. One‐fourth the cost of competing devices. Small footprint. (About the size of an Apple iPad) Advanced safety circuitry of the SYNAPSE controls high voltage to the iFOCUS via separate feedback loops that use dedicated and well‐isolated high and low voltage circuits. Rapid IEF in less than 12 kVh for most samples. Focus Proteomics Exploring the subliminal proteome Figure 1. Patent pending iFOCUS™ (left) with the SYNAPSE™ advanced safety circuitry (right). The iFOCUS can run either 7 or 11 cm IPGs. iFOCUS Foolproof Protocols and Kit Reagents iFOCUS protocols are the culmination of over three decades of experience in protein sample preparation, IEF, and two-dimensional electrophoresis. Focus Proteomics provides optimized protocols and kit reagents. iFOCUS is about one-fourth the cost of competing IEF instruments. iFOCUS protocols are the culmination of over three decades of experience... iFOCUS ideal for small clinical laboratories needing to provide specialized testing.

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Page 1: iFocus_Brochure_Focus Proteomics

iFOCUS: A tablet-sized device for the IEF of proteins and peptides for clinical and basic research applications

Why IEF? Immobilized pH gradients (IPGs) are stable and highly reproducible pH gradients for the isoelectric focusing (IEF) of proteins. IPGs are capable of separating protein charge isoforms differing by 0.001 pI units, a resolution an order of magnitude higher than labile carrier ampholyte generated pH gradients, which can resolve differences of only 0.01 pI units [1]. IPGs spanning 0.1 pH units have been described for the separation of proteins differing by a single amino acid substitution [2].

iFOCUS™ and SYNAPSE™ TechnologiesConventional IEF devices are microprocessor controlled and utilize Peltier cooling systems, both of which significantly contribute to the cost of the instrument, but do not contribute to IEF performance. The iFOCUS is an IEF device with a footprint nearly identical to an Apple iPad. Elimination of unnecessary features such as Peltier cooling, microprocessors, and integrated power supplies dramatically reduces cost and makes the iFOCUS small size possible without compromising performance or capability.

• Versatile high performance IEF at low price.

• One‐fourth the cost of competing devices.

• Small footprint. (About the size of an Apple iPad)

• Advanced safety circuitry of the SYNAPSE controls high voltage to the iFOCUS via separate feedback loops that use dedicated and well‐isolated high and low voltage circuits.

• Rapid IEF in less than 12 kVh for most samples.

Focus Proteomics

Focus Proteomics

Focus Proteomics

Focus Proteomics

Focus Proteomics

Exploring the subliminal proteome

Exploring the subliminal proteome

Exploring the subliminal proteome

Exploring the subliminal proteome

Exploring the subliminal proteome

Figure 1. Patent pending iFOCUS™ (left) with the SYNAPSE™ advanced safety circuitry (right). The iFOCUS can run either 7 or 11 cm IPGs.

iFOCUS

Foolproof Protocols and Kit Reagents iFOCUS protocols are the culmination of over three decades of experience in protein sample preparation, IEF, and two-dimensional electrophoresis. Focus Proteomics provides optimized protocols and kit reagents.

iFOCUS is about one- fourth the cost of competing IEF instruments.

iFOCUS protocols are the culmination of over three decades of experience...

iFOCUS ideal for small clinical laboratories needing to provide specialized testing.

Page 2: iFocus_Brochure_Focus Proteomics

References1. Hamdan M., Righetti, P.G. (2005). Proteomics today: Protein assessment and biomarkers using mass

spectrometry, 2D electrophoresis, and microarray technology. Wiley & Sons, Hoboken, pp 219- 265. 2. Cossu, G., Righetti, P.G. (1987). Journal of Chromatography A: Resolution of Gγ and Aγ fetal

hemoglobin tetramers in immobilized pH gradients. J. Chromatogr. 398 (1987) 211- 216. 3. Smejkal GB (2004). The Coomassie Chronicles: Past Present, and Future Perspectives in

Polyacrylamide Gel Staining. Expert Rev. Proteomics 1, 381-387.

Figure 2. Two dimensional gel electrophoresis of Escherichia coli K12 cell lysates isolates over 1,100 proteins over the pH 4-7 range.

Focus Proteomics

Focus Proteomics

Focus Proteomics

Focus Proteomics

Focus Proteomics

Exploring the subliminal proteome

Exploring the subliminal proteome

Exploring the subliminal proteome

Exploring the subliminal proteome

Exploring the subliminal proteome

Clinical Applications IEF enables unrivaled separation of hemoglobin and transferrin isoforms used in clinical diagnosis. Over 1,000 hemoglobin variants have been described. IEF in the iFOCUS using IPGs instead of carrier ampholytes and optimized kit chemistries provides the highest resolution and reproducibility necessary for diagnostic accuracy.

Basic Research Applications IEF can differentiate post-translation modifications of intact proteins such as phosphorylation that SDS PAGE or LC-MS cannot. IEF is capable of resolving thousands of proteins and this number of proteins is nearly squared when IEF is coupled to an orthogonal second dimension such as two-dimensional electrophoresis [3].

Economical high throughput IEF The iFOCUS is about one-fourth the cost of IEF instruments from BioRad and Agilent. Four iFOCUS systems can run 48 IPGs of varying pH gradients and lengths. Special academic pricing makes the iFOCUS affordable to small universities, colleges, and teaching laboratories. The small footprint makes the iFOCUS ideal for small clinical laboratories needing to provide specialized testing.

Custom 6000V Power Supply iFOCUS and SYNAPSE sold separately or as a complete system with the new Focus Proteomics 6000V Power Supply.

Contact: Focus Proteomics, LLC 32 Overlook Circle

Hudson, NH 03051

Phone: (603) 204-4947Email: [email protected]

www.focusproteomics.com

Proteomics 6000V Power Supply