iapb 2014 vegenov_bodin
DESCRIPTION
Présentation réalisée par Manuelle Bodin, responsable biologie cellulaire à Vegenov, au congrès IAPB (International Association of Plant Biotechnology) à Melbourne en août 2014 : Tissue culture rescue of a wild Asteaceae disapeared in natureTRANSCRIPT
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Tissue culture rescue of a wild
Asteaceae disapeared in nature
Manuelle Bodin, Catherine Gautier, Stéphane Buord
IAPB 2014 – Melbourne
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Cylindrocline lorencei Wild Asteraceae from Mauritius
First observed in XIXth in 2 distinct areas
Described in 1982
Considered as extinct since 1990
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Key dates
Last seeds collected by JY Lesouef, botanist in
Conservatoire Botanique National de Brest, in
1982
End of the 80s, extinction in Plaine Champagne
Failure of cultivation of the plants in all Botanic
gardens in 1987
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The 1rst rescue
In 1990, only seeds still conserved in
Brest, at -18°C
Embryo rescue for 74 seeds unable to
germinate
Only 6 plantlets obtained, 3 survived at
acclimatisation
2001, 3 clones in 2 Botanic Gardens in
the world
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2003 : flowering but no viable seeds
Failure of cuttings
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Cell biology lab in Vegenov
R&D lab,
Suppliyng services for breeders:
haplomethods,
embryo rescue,
tissue culture tools (shoot tip, protoplast, somatic embryogenesis)
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Attempt of 2nd rescue by tissue
culture, with two constrains : Use the less plant material as possible
Avoid genetic variation in tissue culture
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Starting from old stems …
Collect sprouting buds on the stems
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Starting from old stems …
Collect sprouting buds on the stems
Careful desinfection
85 explants plated => 12 survived without contamination
MS salts + Morel vitamines
BPA 0,5 + NAA 0,05 mg/l
Sucrose 20 g/l
AC 1,5 g/l
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A slow propagating process
No elongation of vitroplant stems
MS salts + Morel vitamines
2iP 1,5 mg/l
Sucrose 30 g/l
+/- AC 1,5 g/l
21°C, 16h light/day
1 month
darkness
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A slow propagating process
No elongation of vitroplant stems
MS salts + Morel vitamines
2iP 1,5 mg/l
Sucrose 30 g/l
+/- AC 1,5 g/l
21°C, 16h light/day
> One year
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Getting out of glass …
Rooting and acclimatisation
½ MS salts +
Morel vitamines
AIA 1 mg/l
Sucrose 20 g/l
21°C, 16h light/day
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No soil !
On the way back to nature
The local partner:
2011 : One of the 30 endangered species sent back to
Mauritius
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On the way back to nature
Preparing the last known habitat
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On the way back to nature
Preparing the last known habitat
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=> successful use of biotech for biodiversity
preservation purposes
Questions still to answer :
Will the plants survive in nature?
Will they be able to colonize this place?
Was Plaine Champagne the best habitat for this species?
In Vegenov In CBNB
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New project for CBNB in Haïti
In relation with OPDFM
Juniperus ekmanii
Access to suitable
explants for tissue culture
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Work supported by
Catherine Gautier
Bruno Bordenave
Stéphane Buord
Fanch Le Hir
Jean-Yves Lesouef
Nolwenn Quéniat
Estelle Cueff
Sonia Letty
Emilie Argouarch
Mikaele Paugam
Mickael Euzen
Murielle Philippot
Manuelle Bodin
Thank you for your attention
Une partie de ces travaux est
cofinancée par l'Union
européenne. L'Europe s'engage
en Bretagne avec le Fonds
Européen de Développement.