DIaPARTI\{EN'I' ()tr t\rr(.R()t}tot,o(;\,, os]\rANtA UNM,)tst.l.yI1.Sc. (I'r't,riorrrrl\ll( ll()lll( )1.( )(,\ (Stnrr.ter Svsrcrrri

Sr.llabus, Sclturtr of In\tr.u(lioI arrrl l. xarrrirrrrir,n

Semester l (l5 Wccks)

Pape. Sub.CodcNo.

Sutiecr/l,apcr'i ilte l)struction lxam Maxllrs./Wk I inre IIrs Marks

I

iiItIV

I

Ir

IV

N,IB IO I

t\,{B r02MIJ I(]JNlt] I 0-1

t\1B l5lN.IB t5ll\18 l5.lNJI} IJJ;\,1I I 199

Tt{uoRYCencral MicrobiologyVi.ologylliometrv alrd Computcrsllioohentistr\

PRN(]IICALS(;cIcrnl N,licrobioloS\\/irolosvIlionrcrr) nd (irllpUlcrsBiochcrnistnScminar

ll3

l

4

1

4

t,

(l

4

4

4

100

t00r00100

linn I l2r J 400

Scnlester II (15 Wccks)

Pap€r Sub.Codc Subject/l,aper l itlc lnstruction llxam Maxllrs/\\/k 'I in)c IIrs t!,rarks

I

II1II

Iv

N,'B 20]NIll202t\IB 20iI{A 201

lltEoI{Yirlicrohial Ph) siolog) 4Inrmunology

4A,Iinricrobi.,l n!ur,s lllrd ( hcrr^th(r.rt,t II r,,)llrolo!,\ : rd Iti,.ctr iL.,t Ic(.t,Di,lUc(4

PIIACI'ICAI,S\l icrcl'iill l,lr\ siol,.B\ .llnrntunolo-q)

4A,,tirrirrobi.rl AEs s ird ( trcrrr.,ltr(ri,tr) 4LL,Jrllology and Biochcrnic.rt 'l cctr iouci 4Scrninar 4

l3

ll

6('r)6(b)6(c)6(d)

t00t00100

r00

t00t00r00100

I

IllI\/

t\I8 25 t

l\1B 251t.1Ll 25-1

N.{B 254l\',B 299

I(nirl :l2r 4 .100

Nolc: (a) 'l hc cxlorinrrlion inctudcs bolh MIt tJ I iurd Mt) 25 I sv ihi(t, lls cxanrinrrion inctudcs trortr rVrt rSZ ",,rl

Ufr ZSZ lt,il,,i,i(c) 'l hc cxarrirrrrlion inL:ludcs l)ottr MII t5:t t,nd N,jt] 25:r r;, rbitdr tt,,,,,, '.., ,r, i -.,r,tJ. t,.rtr l\ 1 . ., ,,,., trr, :,a ,, r ,,i,:

I\t.Sc. (l'rer ious) I Semesterl'aper I MB l0l Gcneral l\licrobiolog\ (4 tinits x l5 Hrs = 60 Hrs tenchinB)

Unit IHistory of Microbiolog,r. Micloscopr. Slruclure ofmicrobial cells:

Sponlaneous generation and gcrn thcor\ ol diseasc. Contributions ofAntony van

l.ecurvenhock, Louis Pasteur.llobcIt Koclr. lil\\ard Jcnner, Winogladsky, BeiicrinkAlexarder Flemming, Waksman. I)evclopnlents in modern biology.

Principles and working ofbrighl llcld nricroscopc, fluotcsccnt microscope! phase contrast

,lricroscope. electron microscopc. n pplioatior alld importancc ofabovc nric:oscopcs.

Mensurcr)1cnt ofmicroscoPic olr.jccts. l)rokaryolic cell, Eukatyotic ccll. Organrzrttiunancl function of ccllular otganclls

(lcll u.all ofbactrlia. cell rllenbmrres. llagella. pili, capsule slruclLrre, chcnlicill slrtlcturcol lcptidolrl)'ca11. pr()loplilsls. splrcrr)l)Lrsls. nricrosontcs and ribosonral I{N,,\s. Ntrclcarnratrri3l,rltucieus.

Llnil lllvlethods of sterilization: I)hi sical nrcthods l)rr heal. nroist heat. radiation nrcthods,

Iiltratiorl melhods, cllemical mcthods and lhcir application. Concept oiconlainnrcnllacilit\'. sterilization at industrial lcvcl

MicroLrjal cultures: Concept ol purc cullurc. I\4ethods ofpure culture isolalion,Ijruichmcnt culturing lechniques. singlc ccll isolation. and pure cullure dcvclopn)ctlt

Ircscryation and Mail1tenancc ofN4icrobial culturcs: Repeatell subculluring. prcscrvulion

at to\\,1enrpera1ure, ste lesoil prcscrvalion. mineral oil preservation, dccp frcczing and

liquid nitrogen preservation. liezc-dr)'ing (l)'ophiliTalion). Advantagcs and(lisrd\,arrtaocs of each nlctho(i.

Lhil IIIlderrtificalion mcthods and classilicatioD ot bacteria: -

l\'licroscopic idcntification characteristics. st.lining nlethods simplc staining, diflcrcrr-rstaining. slr\rctural slaittiug and spccial stlirlin!l matltods.

|cological identification mclhods. NLrlrilional (cultural) idenlification charactcrs,

chcmical identificalior charact(-rs. biochcurical idetllificalion nlethods. inlll)unoloBicalchamcterislics, pathogcnic profcrli.s idcnlillcalion, genetic characteristicsidentill.ation.

l'rircil)les ofbactcriallaxononry and classillcitlionr - Nurrerical laxononly. IJcrgy'snranual and its impor'lance. gcucral pro|crtics ol b.rcterial groups.

liickettsiae-Gcncral charactcrs and thcir irr|onancc; N{ycophsma gcncral cllaraoters( hlarrrr diar TIIIC Nltcrxs and l ,( i\'

Unit IVAlgac, Fungi and thcir characters:Distribution of algac, lh:rllus orr:aliz.rrirn"p,oa,.,ion. ar8ir,";,;"j.;"-,;:;l:lii,.,iTj::]'. , .rrsac *d rheir impo.ancc

Vegcrari.vc body offurgi. Rcfrodrclior. tnritirg bodics and dispcrsar offun6alfi"I"iti?,JJ[Xli:1] .t;?Xll: ""u

rrrbit; t r'l ratiorr'r ips o' r'"lil 'n".#",L'iin"n.,*

I Scmcsler III] ISI (;cncral luicrobiolos/ (practicals)

Ceneral instructions. Micr o[,inlorr hhrrandri,rg orrnicrosc.,,;,.'i:,;rb;ii,l;: "l]'l"r\ .nti rrs di5(ipl,,,c

srrirrirrgrccrrriqucs,'u,,,,",,., ,,lli" "rr,r(rr'r,..nrcnlol rrricroscopic ohjects

Ircprriion ofr.cJie.r., ,..,".:, , . '','t,r"' 'ltllLttr'ti rl rrrd spc.r,l srrirrinl's

Stcri lizrlion 1lr occdures,nr.tL,ul.

l50lfllior altd culti!alir-lr of prrrr.. r.rrltr.r.t.ldentification tl)elhods ol bil(t(ri.rlsolationanJ culturing oI lrrnl.i rr c.r,r.,rrr.l rrr.,l,l. r.rn.i lrlt,.rcObservation of spccinren and |cnrr.rrr,rrrt .lr,Jc.

Rccommended Irool$

Text book of Microbiologl. br Nl. Rrrrrorrs- Ueneral Microbiologl b1 StaiDier. I\.LrJr,, .rnd A.lcliergKevret! oInredical microbiologl. b) Jrrr irz. rrrclrricl "uJia"tu.rgtsaclcrial and.Mycolic infccriorr..,firnn. I J Duhos aod f f irri f injn"ntt'rtncrptes ofMicrobioiogv and lnr)rur,,l,,s\ n1 i,:,ri.. n,,ir*..J. iilon. cin.u"rgu,,a

Microbiology hy pclczar N.l.J . l(rcLl. t(l) : r.l( 1r.,,). l:(.sJnrclurcand llcprodLrction of AIJri,c. \,,1. I at ll l)) trirsct,,t..t..lnlroduoion lo Algoc hv Nlrrnrs 't

l\oducts arrd ProJrcrric: oi Al;.r.. I,r ,/rz:rrl-rcsh,walcr algac oflhc Unircd St,ric:. tr Srrrir)r tiM.I nlroduclory M) colo!). b1 Alcxo1,nly5, 1 1urspersal in Fungi by Intold. C fh,licrobial physiology hi Moer

Li::o:,,y",,1" by Scotcl \\ rrr,r \..,,,.t)e r:,rt, l,Ji.duururor)'hxIc rDr.rrrs in Mi,..ol,iulL,l) l,) (r,,lr.rl I(c.ldy (.t Iltirock's Diolof) ofrrrielooreirrisrr.. l,\. l\l:.lirj,r, j\tl (l irl

Nl.Sc. (l'r'tl ious) I Scmcs(crI'aper II MII 102 Virologl (.1 tlllils x lS lIrs = 60 hrs tcaching)

Unit IClassifiCation ofviruses, Melhods ofcultivirtion. dclection, quafititation. Propagation and

maintenancc of vimscs (bacterial. planl and aninlal \,iruscs). Slructure and replication ofplanl\iruses{a) IMV (bl( rulilln\\(r rr,'r:'r.' r'irrt:.

IInit IIStructure and replication of bacleriophagcs: L)'1ic ds lincar l)NA viruses (12, 17);

Llsogenic ds lincar DNA,ti$s (i,anrbda): ss( ircttlar l)NA r irus (i x l74); Male

spccific filamentous ss((N,|)r,irus ilrl7 urrtl lr'1Ii)

Unit IIIR(conrbinalion in phagc. rnulliplicit! rcrcliyxlior) anLl lherl(rt),I]ic nlixingStructrlrc and replication ol aninral !inrscs I^(lcoo\irus (eg. l1'pc2)

Unit I\/.Slructure and r eplication of N{)'xo! iruscs (cg. Llllucnza); l'ox virus (eg Vaccinia);

Ilcpatitis virus.Effect ofaninlal virus inlection on host ccll: Viral interference and interferon; Tumorviruses (DNA and RNA)

I Scnrcstcr' l'nl)er II Ntl] 152 \riruloS] (l'rncticals)

isolation ofphagc from soil.Cultivalion and prcscNatiolr ol phngcs

QuaDtilation of phagcsGro\(h phages ofphagc ar)d bursl siTc

Isolation ofplaquc lyl)e arrd host rangr nrlrlilrrts

l'hagc inductionLysogcny-Transductionl'ropagation ofaninral viruscs in cgg allitntoic, amniotic.rnd CAMPropagation ofviruses in tissue oullurcllaquc titration and ncutralizalion

Qua,rtitation olYinrs by HA,l\rck titrittio , plaqLlc litralionidenlificalion oI vir,trs I IAIl).nlonstrltion ol' c) lopnlhologicrrl chirrritcs

Svr|l)tonutic obscryalions ol l)lurrt Yirrrl irricotiotrs(llrloroj)last agglulirralion oI Ililnl Yirtrsrs

Rcconrnrcncled Ilooks

Ccncral Virolog) by Luria and Damelv lrolo8y and.ltununolo,,t h. l^r r,

I:]l,l:ll,or vi,orosr ui'nr,.a". ",a

Van lurcrrrrsll v trolo!) b) Sntilir

i,:l::i:::b":",i",.nd rrreir \iruscs b] s Harer'.rur(culrr lrrolot) ofthe gcne b\ War(A IaborarEor) suiac in,,;,ii,1,. i,l,ilill Hoherts Stair.t and \\'crncrrrus,c rrL, pr.,..:,rure, i" ;;il:;,,:r' ilifl:tJ] il:11,,X,,,.ll1,,.,

u"r"',,f'itl!f, ::ious) I senresrcr

,,, ",,,,.,,,, rr,11,1,,Ji,;l

i,i:ll,:,,1,1i|,,,.^{rnil I

lj]]]::1.:: , ',, r,, srrri,ri(s i,,.t r,iu,r,err)

,,i,li;ill,ll;,:lilill:i,.lli,i^lllli.H:"i,:::::v,ri.rb,r.. rari,,i.rr,.i,r.d lrcr,'cncy

l J[[T :tii:,m 5E1 ", :*;::J#t::r ;:k][tilii,i;

"Uni( II

$*fini*g*,**,i:**'m* ,, -,."1,, ^. ^."'^

Urril III alions to biologl and nticroltiologY

lnlrndLrclion to C,,D)lnrlcr.-lr,lsrc colcclrls (ir)t)lll. r)l hIr a,rrt r

^ r

,il;: ................1;i:,,,,,,

j;;;l;;i ;iiili x"\ l"i; iill'lT;L:i::1,,..B:i.tf;; :lll .,i,iil l,,i;lJli,

ilt:lfJi l,*, iit]rii:t! li,Y,t,fl !,; ::],T,,ills,r R CD.RD.D,'

',,1,r. .r,. .,ii,,,,'iii.::';;".;i",i:::,"J::,u,op. Fi/e .rrr.r r,rJcrs

l,lll],,;i:',,,,,,i;,;.i,,,,ri;li'ii,iiili,ll"f,lli,ll'1,:il.,ii:;i[ft:,J;i;,i

Unit I\IWoiJ lro""..lng, Opening, creatirlg and saving documcnts Tlping' navigating and

."f""in g "aiti,ig

*a sorrilg-checking spelling and grammar -.fomlatting - changing

oppr"."i* "f

y"u, p"ge i-lportlng giaphics - workiig with tables - wo,rkine with land

documents PdntlngDiectronic Spread S}-reet: Creating, opening and saYing files - working with worksheets

ona *ort toot, entering data a;d s;lcc1ing cclls - cditing rvorkshects - printing'

"r"oti,lg io.nrrt"" rvorking uith charts - Sunrmarize data in lists and tablcs - Analyzing

.lata wih Pivot lablcs - A[alyzing sarnple statistical data - Validating cells'

I Sctuester Paper lll tr{l} 153 Iliometq'and Conlutcrs (l'racticals)

irorn)alion or frequeltcy dislr.ibution, graphical prescnlation a d calculation oldcscriptive

I;itling oldislribuliori lJi onlial, Poiss.n rnd Norrrral distribuliorl

Chi-square lesl, goodllcss offit. contillgcnc\ antl hclelr'rgcnci1l

Snlall and largc sanlpie tests, sample mcans and froperlicsI)roblems oD corrclation and reglession

ANOVA Onc rvay and two rvay classilications

Creating. doculrleDts usit'tg word processor

Exercising the fcatures ofword processor

Creating sprcad shcets

Usage ofspread shect to biological applications

Reconlnl(nd(d llooks -

lntroduotion to the theory ofstatistics by Alexander, M Mood and Franklin

Fr.rndamcntsls of Biouetry by L.N.IlalamStatistical nlethods by Sledecor and Coclrran

Intloduction to computcr and its application by Chac C'Chien

Basic Progralrrrring languagc by llaiaraman

n;o,r"iisi-., - a ,rr""nuai of statistical methods for use in l lcalth' Nutrilio11 and

Anthropolog) b1'K Vishveshwar Rao

M.Sc, tprcr iuus) I Scn)(rlrrl'alcr I\' l\ttt ll){ ltiuclcmi\ln t4 unir. r lS hrs = 60 hrs tcaching)

Unit IpH and ils bioiogical rclevanccDclerDlination of pH. prcf aratioD of buffersConcepl ofentrop). frec-cnr:rl]\. free eEq,iri;,i;';;;Ji;;;... I..j"^"i,.,ili,l[.*,],iiiTili,llltilliislcompounds

Unit IIIliological oxidarion, IlioroSicar redox ca.,iers, biorogicar membranes. eleclro,rransporr. oxidarir e plr(,sJ)lr,,r \ l.rr ro. .nd ,*f

"rir^-.1f";,",;i ,i,"iii, ii..lilFhotos) nthelic clcclrtln lriUt\r)r,fl

llnir IIII ipids clafficcrion: ll.rLril.rl liliilr. nr.,otttiologi"al i,1port,,,)

'^"_' "r''ui la(rslaglandins slrtlclurc' l'unclion l\'laiol slcroi(l\

I[l:il:]rr, Srftrclurr rnLr t)rutrurri(s ofprrrirc\, pyrinri<tines, nucteosides and

Mctabolism of putiDcs alcl pvr inritlincs Biosynthesis flnd dcgradation

Unit lVl'rotehi and anino acicrs l'rofq-nic5 6 f nmino acids. structurc, confimration andproperties of proteins, nrcrabolisrn of arovervies; Urea cycle

nino acids' biosynthesis and degradation - an

I Scmester Paper I\/ MII j5,l Iliochenristry (practicals)

f,rcnaration of bufr'crr anJ :rJjrrsrrn.. r ur.pl I

Vualllattve tes(s fot c.Lr l,r,lrr tlr,rtc..rDd rrnail.sis,rI rrnlrro\ rrsQulrlitative tesls f,,r n.nir,,, e. i,l, lrr,d ,.nlrlr..j. of urrklou.lrsI csts for lipids (qurlilijlir,JtQuantitative estinlalion ol. glLrcosc and frLtctose

Iicconlmendcd Ilooks

Iliochemislry by Lchninr:cr

9]1,1,J-:,".t &l.,n""lisrrl r,1 r.,,rrrr anLl srumph

rrocncmtslD ofNuclcic a..itls h1. l)avidson,rologtcal (-hemislr) b1 MtrlluI n J t.ardslrroclrcntistr) by \\'lrirr.. IIrr cll.. rrrr.l SnrrrLl\4elltods in l:nz\ ntolo!r :(.,rc\I lrc Ccll - Bratch anrd hjrr,lr seIic:

l,.,blr-torl crt .,rr.,-r,r . r.. i\tr.r, ,,i.t.r,\ r,t C J,.rl Rcd.tv,.l .,1

"r( ( r,'Itrll! lh\ .. .lt.il '.1 . 1 . t . , , r , , r .

, : , t

]'l.Sc. (l'rc! i0tr\) II !cmes((rl'apcr I MB 2lll Ilicrirhi:rl l'hr.irrlfif ti unitr r l5 hrr - 6ll hrs tcaching)

Unit IMiorobial nutrition Elemenlal nutricnt rcquircnlcnts ol microbes, nutritional groups ofEacteria. The autotrophy Photoautotrophy and bactcri6l photosynthesis

Chemoautotrophy and autotrcphic mctaholism.

Concept ofheterolrophy - Photoheterotrophy and chemoheterolrophy Heterotrophic

metab;lism in bacteria. Respiration (Aerobic and ana0robic) and fermentation

Unit IIMicrobiological media and culturing and cultivation of microorganisms - Autotrophic

media, defined synthetic mineml mcdia. heterotrophic media The concept ofnrolotroDhs and auxotrophs, prototrophic (minimal) media (defined media), complex

media (undefined mediat. Basct medium' enriched media' enrichment media, selective

media. biochemical media, ditlerential media. maintenance media' transport media'

Media for cultivation of fungi, and algae

Cultivation methods of bacteria, slantculturing. stab culturing, agar plate culturing' rolled

tubefuottle culturing, tube cultures, flask culturing'

Aerobic cultuing methods, anaerobic culturing methods

Envtonmental requirements of growth

Unit IIIMicrobial growth: The concept ofgro*{h and dcfinition' lormation ofprotoplasm'

tuiiaing oi.u".o.ofecules fiom eiemenlal nut cnts, supramolecules' orgnelles ofcell

and celiular components. Cell cycle in microbes and generation time

i-rrth rhur", oibo"teria Lag phase, exponential (logarithmic) phase' stationary (ideo)

nirse. decline and survival ofmicrobial cells lmportance ofeach groMh phase -ivnch-ronous cultures methods of synchronous culluring

i'oiiinrou, cuttrting merhods. l'actors cffecling growth Melhods ofgrowth

measuement.

Unit IViut*" -a prop".ti". of spores: Bacterial endospore structure' phenomenon.of

,ro*triion, Uio.t,".islry and gcnetics ofsporulction' lnduclion ofsporulation

nh"noaanon. Cerminalion of sporcs '

ii" .on""pi or ait.".e: lnlcctious discase' palhot(nic nlicrobcs propen-ies of

oathosenic microbes, Inltction. palhogcncsiq rnd virulcncc' Virulence lactore

[lii,r"#;;,;il;, bacterial tox]n" irotoxins toxuirls cndutL'xins' enlerotoxins

iiir,"i"nt "ii"-i, ,roduclion' Fxlraccllulur cnr) mrs of pJlhogen-ic ^bacteria

er'rri.rii,ill "ii"-rrl "nd to"i" '-n'ynt'-t'

laboratory tcsting methods for bactcrial

virulence proPerties

IlSemcstcr l,rDcr I MIl25l Microbial physiology (practicals)

Prcparation of microhirrlosical mcdia*.j"r,"J,,.ai",

""lr"iii;l;iiili::".:l,i..llillflJ:Jl;r'" ,,,",,,i,r media. basic media,

lsolation and cultivittii,I r,t'ulttorr(,phic nI(roh(s

:r#;:ifljJil"rl:r., nricrohes 9cnr and r(ab curtures. tube cutture, flask cultures,

Anaerobic culturing merhods. ana(rohit jarand irs usc. pyrugallol method.thioglycollate media culruring. Jnaerohic glorc hox anJ Ii. "riif"",r""

"'"".MicrobialgrowhexperincnG Vi,rbt"cletermination count ol.growing cultures and generation timeDetermination oimicrobial growrh b\ turbidomelric methodsStudy of bacterial grov,,th cuieFacnues effecling lhe microbial erowthTests for microbial toxins . dereirinarion of L D50lestlng lor extracellular microbial enzymes

Recommended Books

Text book o[Microbiology b1 M Bunows

S:.l:].ilyll*i",:rf b1 Srarnier. Deudrofr and Adetberstrevrew ot medrcal microbiology by Jau itl. melnicl and idelbe.nBacteriat and Mycoric infections ofman r.a. rjrr". *j ril.ilirl".",,Principles ofMicrobiology and Imnunology Uy Oavis. Dulbecco.. fiion. Cin.lerg u,aText book of Microbiologv by nanthanaravinin

lll*"Pl"]rjy by Petcz.r M.J . Ried. RD ;d chan, ECS.Mrcrobial Physiology by Moar.Brock's Biology.of microorgan isms by Madrgan. MT et alt rochemls{ry ol-baclerial growth b1 Mandelsium, Mc euillon and DawesBacterial Metabolism bv DwellelrPhotosynrhesis by Dewlin and Barker

3TIl,^?U:ry,,rn."nrs in.Microbiotogl by Gopal Reddy cr alvlcrobes ln Aclion by Seoley HW and Van-Demark- p.l

M.Sc.(Pr€vious) lI Semestcr

Paper II MB 202 Immunolos/ (4 units X l5 hrs=60 hrs teaching)

Unir I

Briefhistory ofimmunology; Antigens - isoantigens, alloantigens, haptensi Antibodies structures,genes; Structural variants, classes, and subclasses ofantibodies; Immune system - cells, vessels, organs;Immune response - antigen recognition; T-cell interactions; B-cell interations.

Immune response; Types of immunity _ innate, specific, acquired, prophylaxis; Vaccines - whole cell,subunit, recombinant protein-based, DNA-based; production ofvaccines; eA ald eC ofvaccines;Accepted pharrnacopeiar techniques: Future ofvaccines - bacteriar and vimr vaccines in market;

Advantages and disadvantages ofvaccines; Approaches ofvaccine design; Acquired and naturaltolerance; Mechanism of tolerance; properties of interferon; production and assav of interfemnc.

Urit III

MHC Complex; trnmunoglobulin superfamily; Selfand non-selfrecognition; cenetics ofdssuetransplaotation; Cenetic control ofimmune resF,onse; Macrophage r€striction; Structure ofMHC; MHCproducts an(cell surface; MHC and tissue transplantation; Human MHC and disease association;Autoimmunity and HLA; Exa$ples of autoimmunity eod treatnent; Inflammation and anaphylacticreactions; Immune reaction of infection, reactions to infectious agents

Unit tl

Unit lV

Antigen-antiHy resotions; Polyfunctional antibodies; Cross reactivity; primary antigen_antibodyreactions ir vr'eo; socondao/ sntigdn-sntibody reactions r1z viuo; Sensitivity ofantigetr-antibody-reactions;Immunological methods - agglutination, precipitation, complement fixation, radioactive and enryme_mediated amplification for det€ction ofa igen-anliMy reactions; Immunization _ adjuvants,lymphokines; Altemate pathway for complement fixation; protective immunity to infection; Role ofimmune mechanism in protection against infection; Immune effector mechanisms in specific infections;Evasion ofimmune defenses; Tumor immunity - host-tumor relationship, tumor immunity in humans;Tumor antigens; Mechanism oftumor destruction by the immune system; Tumor surveillance; Immuno-diagnosis or cancers; lmmuno-prophyla-ris ofcancer; Immuno-tierapy of cancer.

lI Semester paperll ivll252 lmmunology (practicals)

Agglutination reactions Widal. VDRI,. llA. Blood group/t1,pingPrccrpitalion tesr: Ring interf'hasc. sinplr r.r.licl Jilfu.ion. r,rr".tis.lqnl.inununuclcctrophoresis1\\eutralir,af ion te$ - Plaque neutralizltion. IIaeme absorplion testRaising ofantiseraSepcration of serum, WBC, RBC, plasma, CBI) and differential blood pictureI ymphohlast trnns[ormation. Jermc plaquc rlJ{I. I-cell miBralion.Sepcration of serum prolcinsBlot transfer and detection ofprotein on blot by stainingLymphoc),te viability tesrELIS, Indirect agglulination (a) Hepati(is (b) pregnancy hCG AgRaising ofpolyclonal Ab and MonoclonalAb -

Recomnrcndcd Books

Immunology and immunoparhotogy by Stewrn SellCellular and molecular immunology by Abul K. Abbas er alImmunology by Herman N. EosenTest book oflrnmunology by BarretMolecular basis ofimmunology by Constantin BenaImmunology - The sciencc ofself-non sclf discrimination by lan KleinEssential Immunology b) Roill. lVImmunology by Kuby, J.

M.Sc, (l'rcvious) ll Semestcr Ptpcr III MB 203 Antimicrobial Agents andChcmothcrap) ({ units x l5 hrs = 60 hrs teaching)

Unit IHistory ofchemothciapy. lypes ofanrimicrob'ial agents. Plants ahd arsenicals asthcrapeutic agents. Paul Ehrlich and his contributions to chemotherapy. Developmtnt ofslnlhetic drugs. Jevelopment of anribiorics.Chemical non-medicinal antimicrobials- sanitizers. disinfectants, aantiseptics.Selccli\e toxicity md larger sites ol drug actionSynthetic nredicinal agenrs {drug:.)- sulphonamides. antitubercufar compounds.nitrofurans. nalidixic acid, melrorddazoles,Antibiotics - Definition ofantibiotics, types (chemical) ofantibiotics, cell wall inhibitors,membrane inhibitors, inhibitors of macromolelcular synthesis, antimetabolites.

Unit IIBactericidal and bactedriostatic agents, Factors affecting static and cidal activity,phenols and phcnolic compounds, alcohols, halogens, heavy metals, dyes, detergents,aldehydesNon-medical uses of antibiotics.Assay methods ofantimicrobial agcnts Phenol coefficient, qualitative assay ofdrugs(drug sensitivty testing), quantitative assays liquid tube assay (MIC), agar tube assayAgar plate assay.

Unit IUPrinciples of chemotherapy and Drug ResistanceThe physical and lab djagnosis, tests for sensitivity, choice drug determinatioq, dosage,

route ofadministration, combined drug therapy. Policies ofantibiotic usage. Antiviralagents - Biological antiviral agents- inteferon and its action, chemical antiviral agents.Phenomcnon ofdrug resistance, basis ofdrug resistance, biochemistry ofdrug resistance,genetics ofdrug resistance. Control ofdrug resistant bacte a

Unit lVMode ofaction ofimportant drugs - Cell wall inlibitors (betalactam drugs), membraneiohibitors (polymlxin), Ribosomal inhibitors (aminoglycosides steptomycin), folicacid inhibitors (sulfa drugs). antifungal drugs (nystatin).

ll Sencstor l,aper lll MB 25-l Antintt"""l''iiin'u'

ut"'ts and Chcmothcrapv

Testing fbr drug sensiliviryDeterntination .f M IC values (rube dilution method)I)ctcmlinalion ot phcnol coctficierrr rReidel Walker coefficienr. RWCr5ludy lorantimicrobial spcctrunt of anlimicrobialsDelerminalion ofcidal and slatic acli\ir\Scrcening for antibiolic producing microbesChemical assay methods for anriiricrobral drupsMicrobiological assays of antimicrobial drugs

Rccommended books

Disinfecrion. srcrilizalion and prescnarion Ed. Block. SS.Microbial contamainarion conrrol l-aciliries Ed. Run_k.le, RS and philios- GBPrinciples and merhods ofsreriliTalion in heotLh science Uv pi.ll". i.L" l"Inhibition and dcsrruclion of the microbial c.ll Ed. H;;;'Biochcmisrry ofarrimicrobial acrion by Franllin. IJ arid Snow, L.lhc Molecular basis ofantibiolic iction by Cale et alAnribiotics and chemorherapy by Cenod et alPharmaceutical microbiologoy by Hueo RussellMicrobiological assays by FIewinAntiviral drugs by S.KargerBurgers' medicinal chemisrry (all volumes) Ed Manfield E. World.Antibiotic interactions by WilliamsThe control ofantibiotic resistant bacteria by Harris and Harris

M.Sc. (Pr€rious) ll Scmestcr l'aper I\: i\rB 201 Enzymolollr'and BiochemicalTechniqucs ({ units r l5 hrs = 60 hrs teaching)

Unit I[.]nzymes nomenclature, classillcalion nlethods lor dctcrmination of cn-zyme activityIsolition and pirrificatioh olcnzymes. Ilnzyme kineticsi Ellect ofpH,'substateconcentration , temperaturc ard inhibitors. lsoenrymes. Competitive and non-

competitive inhibition

Unit llMcchanism of enzyme action Aclion of fubonuciease, chymotrypsin, and tlvpsinCoenzyme catalysis. Mechanism ofPAXPO and thiamine pyrophosphate enzymes

Control ofenzymes. Regulation ofenzyme activity: allosteric enzymes and feed back

mecha.isms. Metabolic compartamentalization in relation to enzyme, enzymes and

secondary metabolites

Il Semester Paper lV NIB 25,1 Enzymolog/.nd Biochemical Techniqucs(Practicals)

Dctermination ofsaponification value and iodine number of fals

Quantitative estimation of inorganic and organic phosphate

Partial purification ofenzymes (B-amylase, urease, pyrophosphotase and catalase)

Effcct of substrate concentration. pH, time and temperature on enzyme activity

Calculation ofKm for partially Purified enzyme

Inhibition of enzymc activity

Unit IIIOptical methods: Elementary treatment ofbiochemical methods, colou metry and

spectrophotometry, flurometry, optical rotation, fl uorescence

Seperatign methods: ChLromatographic techniques paper, thin layer, ion exchange, gel

filtation and affmity chromatogaphy. Diffusion, dialysis, cell disruption methods,

centrifugation techniques, cell free extracts and their use in metabolic studies.

Unit IvGeneral method ofstudy of intermediary metabolism

Electrophoretic techniques and application, counter current distdbutionRadio isotopes detection alld measurement ofradioactivity - scientilation counters,

autoradiography, stable isotopes and their use. Uses ofmutants in study of metabolism

Rccommcn(led llo(,ks

lliochcmistry hy LehninSerOutlines oflliochnnistry by Cohn and Slunrphlliochemistrli.of Nuclcic ucids by Daviclsontliological Chenristry by Mullai and CjardsDiochemistry by Whitc, Ilandler and SmilhVcthods in Enz)mology seriesI hc Cell :Bratchamd Ivlirsky seriesLaboratory expcrimcnrs in Microbiology by (iopal Reddy et alBiochemistry ltb rniurual by Jayaranran

l)hl'\lt I \ll,lN.l Ol )ll( ltolll()l.o(;\. Osll-,\){1,\ trNlVI,lltsl l'r'll.Sc. (l,ina1) \ll( lt()ltl()1.()(;\ (Srnrestrr S\stcnr)

51'll:tbrrs, Schtntr. ol Irrslru(lion :rn(l I,l\xrrrinlllion

Se estcr lll 115 \\'ecks)

Papcr Sub.CL\lcNo.

Subicct,'l'apcr lirlc lIlJlnrctior) Iix.nr iUr\I lrs/Wk I inrc Ilrs Nlnrks

t

IIIIIIV

100

t00100

100

Mts30INrr] 102MR lr0 iMa I0.1

IHLORYNlolccular Biology and :!licrcbial Ccnclics 4

IndLrstrial Microbiology 4

Soil illicrobiology 1

Nlcdical Bacleriology 4

PIt.\C I IC,\I,Siuolecular lliology and l\'licrobial (;coclics ,l

lndustrial l\{icrobiobgl- .l

Soil Microbiology .1

lvlcdical aacteriology lSeninir 4

lolrl lll.l

ll.l

:l

b

d

I

IIIliIV

NIB ]J I

ivlB ji:t\tB:l5lMB 3J,IMB J99

100

Scmesler lV (15 \\'ccks)

Paper Sub.CodeNo.

Subjcc/Papcr litlc Inslruction Exam MaxHrVWk Time tlrs Marks

III

I

1V

I

I]

lllIV

i\,1I1.101

t\'18.102NlB.l0:l\1ll .10 I

THI]ORYMoleculir Biotechnolo8y 4

ivicrobial BiotechndloSy .1

i\licrobioloSy olFo l flnd llnviroDnler)t 4

Mcdicil Virology an(l I'rrasitolos), ,l

I)ll.i\C ll(;\l.SMolc{"ullr tiiotcchrolo8y ,l

Microbirl lliolcchrrclogy Jiuicrobiology ol l:ood ltnd l-nvironmcnt 4

Mcdicrl Virology and l'arasitolog,y ISeminnr 4

l3

l

100

100

100

t00

Nlti -li I

vtB 151ivlu,l5.lN{B 454Mt] 199

(,(r)6(b)6(c)6(d)

100

100

t00r00

I otrl .... .12 [,1 .100

Nole'[he strdents of M.Sc i]ilral Micr(rbiology hrvc k) undcrgo an cducalioDaltour/training/visitto sub.jcct based induslr ies/institrllions arxl s|bmit a rcport as parl oftheir ac.rdemic curricuhrur.

(a)'lhccranrinnlion inclLrd€sl,olhNII] l5l lnd Ml] 45l syllabi(b) l-h€ c\xnrinirtion includcs both Ml] 352 :tttrl Ml) 452 syllabi(c)'Ihc c\amination irrclLrdcs both Mli.l5l turd MB 45lsyllnbi(d) lhe examination includcs both Mt] l5,l lt d MB 454 syllabi

M.Sc. (Final) III Scmcster Microlriotog).Paper I MB 301 Molccular Biology antl trIicrobiril Gcnetics

(4 units x lS hrs = 60 hrs tcaching)

Unit IStructLrre ofDNA and its biosynthesis -. Detailed structure ol DNA , variation ftomWatson and Crick model, Z-DNA, A & I] DNA. denaturation and melling cun.es.Geuome organization in prokaryoles and eukaryoteslinzymes-involvcd in replication, step by step process, hetcroduplexes,Modcs of DNA replicatiorI- Semiconscrvative ancl conservativeMechanisrns of I)NA replication in E.coli (bi directional), Mitochond.ial (D_loop), ViralDNA (ltoi.l.lNc CIRCLts), Single srranded DNA phages ( C X 174).l'r.LJr)'(,ric lclurncrc ind its replicarion. Selfi.h DNA. A rr .ic.lucnces

Unit IIRNA structurc and biosynthesis: m-RNA, r.-RNA IJINA structLrrcs'ltaoscription apparatus and proteins involved in transcriptiont'rokaryotic and cukaryotic transcdption. processing of FRNA, r_RNA, mJiNA splicingConccpt ofribozyrneGenetic.code and Wobble hypothesisProtein synthesis - Translation in Prokaryotes and eukaryotesPost translational modifi cations

Unit IIIMutagenesis- Types of mutagens, molecular basis of mutations, analysis of mutations,sitc directed mutagcnesis and reve$e geneticsDNA damage and rcpair me6hanisms. Mutagenicity testing using microbial systemsConccpt ofgene Bcnzer's fine structure analysis, introns and cions.Complementation and functional allelisnl

Unit lVDdctedal translornlation and rccombination- l)iscovcry, detcction, tnolecularmechanisnrs of trallsli)rmatio11, transformation methodsBactcrial codugation Sex factor in bacteria, F and HFR trurlslcr. nlechanism oflransfcr, Linkagc ntapping, Mcchanism of recombinationBJcterial lrinsdLrction trcnsJuctiL,n phcnumenon. methoLls ut lrJnsduction, co_lransduction, generalized, specialized and abortive transdLlction, sex-ductions, anil theirapplicationsGenetics of eukaryotic viruses

I'rpcr I Ntll -151 Molccular lliolog-v an(l I'litrohinl Gcnetics (Practicals)

Ir\trac(iorvl\olation ol DNr\ (Plasmid DNr\ ANI) grnornic l)NA) (Mini prcparation and

largc prcparationE:itimalion oil)NA. RNA and Protein (colourimctry)

Determinalion of molecular rveighl'of DNA. resolvcd on ag,arose gel electrophoresis

Dctermination ofmolecular weiglt ofprotcin br" I'A(il:InductioD ol mutalions by pl, ysical/chemical mutagcns. scrccnil1g and isolation ofmutants, lleplica plating technique'Iranslbrmation in bacteria

Cbnjugati(D in bacteria

ComplcmcrtatioD tcsts in bacteria

llrotoplast prepilration and rcgeneration

Reronltllcnrlcd l)ooks

Ivlolccular biology by David and FrcifclderMicrobill gclclics by David and FreitildcrGcnctics ol brctcria and their viruscs by William llaycsMolecular biology ofthec gerle by Watson et al'l hc Ilioshcnristry of Ducleic acids by Davidson JN

Molecr.rlar hiotcchnology by Primerose

Molecular []iotcchnology by Bcmard R. Glick and Jack J Pastcrnak

f)NA Microlrrilys Ild. IvI. Schena

- M.Sc. (Final) III Semcstcr lllicrobiologPrpcr ll Mli 302 Industrial Microbiology (4 units r 15 hrs = 60 hrs teaching)

Unit IIntroductior to industrial microbiology Dcfinition, scope, history, microorganisms,

propcrtios a|d industrial products

Screcninglirrrrticrobesofindustrial irnportance. Prinrary scrccning, scrccning for

anlvl.lsc. orllxnic acid, antibiotic. atuino acid and vitarnirr producilg microorganisnls-

Sccondrrv scr.tning. Iurthcrevaluation of Priolary isolxtcs

I)ctcction r d xssxy ofttnncntation Products. I'hysico-chcrrricxl melhods and biologicxl

assitys. l;cnncnlattion equipment and its use. Design of tcrmentor, typcr ol lernlentor,

rgilillion, ucretioll- anlilbam, pll and temperattlrc control

Unit I1

Inocularu rncdia, irtoculam preparation

Rawlnatcrials Saccharidcs, starchy and cellulosic nlaterinls

['crmcntation mcdia and stedlization.'Iypes of l'crmcnlations processcs Solid state, surfacc itnd submcrged Lrmentations

Unit IIIUillch. lcd balch and conrinu()us Itrnrd.\(.t.,f ,,t ri.r,,,rrrri,,.\ l,ruJL,cr.,."1:,i ll.,li:,']'

turl orrnrrhir\lc rcrr.(.,,:,ri(,r.Ircrnrcnlalion lypc rc:rclions, alcoholic_ f.ii.,*,J.1 ,*.a acid, propionir.:rcitl. burantlioland acctonc-butanol typcs

Unit lVstrain developmcnt strategies. Enviro.lnentar factors and genetic ractors forrmprovemcnl. lnrnrobilization methoA. +Uso.ption, corai"ri f,ril.r"",

",ii.r,rcru ""Uclosslink,rgc.t1pci.'l,carrlcrs.,lLi\JnlJ|firnt]disadrantapes

pxpcr II MI).152 In(lusrrixl Microbiolory (practicals)

Scrcerring for irn)ylis,j 11r,,ducinp nt I.tIrsntiScrecrring lbr urgunJe .ruiJ pro,lrrcirr! rrricr.,,or3.rnisrr.lsolation of antibiotic lror.luciI]r micruirlr,,rution "nJl,,rtffi;;';;;i:

"""'*tt-isms bv ciowded plate technique

Scpcralion of ;rmino acids by chrornrtugrlphlEstimation ofglUco\c hy DNS merhodEstimetion ofethunol by Jichrom.rrte rnelhoJExtimation ofnlaltoscImmobilization of microbial cclls by entrapment method

Recommended Ilooks

Industrial Microbiology by Casida. LEIndustrial Microbiology by patel, AHIndustrial Microbiology by lvlillcr. B!l and LitskvInJustrial Microhiolopy hr l,rs;rrrr.rrrrl Drrnn\licmhirl Tcrhl-t,'f v hr l,-1 1.1.,. t, l .rrr I pr r rrrr.rn, D.llrL,cl,(Iltstryi.l'lt),l.lslri:tl \lt(r. nl. i\ ,{ h\ R,rinbowiItLl Rol.Ilconomic Microbiology by Iiosc \iol I V

I::::ll;:l llllrll:':,,d rr,.,rr(r,,,or,,sv h) r osrr 11 \\ M and Ke y. c rr onlTr('llcrlsl\i llrnlc(lrIl.,lugy. AJI rolt.rnrs llJ. Murrav Mon-V^".lJioluchnoloogy (n tcxr hook ot.in(lusrri.rl l,ti..oUiotogyi

'eji.u"c.u, a Crur*Advances in Applicrl Vic rubiology l.d. pcrlman S"ri", ot roturi!.-' * ' ' ""''

LaDoralL,ry cxpcflrn(,nts irr ntierobiol,,gl by Copil Reddyel al

M.Sc. (Final) III Semtsl('r iUicrohiolog)

I'nprr ltl IIB -103 Soil \licrohiologl ('l unils r l5 hrs = 60 hrs teaching)

llnit I-fhe soil as habitat [or microorganisms: gcrrtr'rl dcrerrptrunulsoil soil slructure'

diffcrcnc...'iinlr'ng soils rlld facl{\r\ ol-((olof i(.rl'lrllriruanceSoil Microorganis-ms: Distribution' abundancc' nlethods of cstimation' biomass

a"uaur",rr"nl "nuironmelrlal

factors. activity lLnLl linctioDs o1'soil bacteria' fungi' algae'

nrolozou, lrluc green rlgac and 'oil lenility

Microbirl ,livcrsity in soil anJ ils signilicJrrce

Unit IIOrganic nratter clcconlposition both nalive iind ilddcd organic matter and factors

,ov,:nrinrl thc,.l,:comPositiolrii".r" "ri.,r

nl cirrbonaceous mJt(ri:rls irr snrt ('llrrluj(' hcrniccllulose cnd lignin

JGmpositi.,n, thctors goveming thc rlecorlposition and [riochcmistrl' ol decomposition

Irlinc,cti,ttlnn or ninogenous orgcntc matter nricrobcs involved and factors influencing

the processcs. Soil humus lomlation

UNit IIINiirin.rti"n N1icrobes invol'erl. iaclors inllurncing ritrification, nitrifying bacteria and

biochcmical mcchanism Dcnilrification nricrobcs involved, factors influencing and

the mechrnism of denitrification and nitratc follutionNirros.cn tixation - Asymbiotic and symbiotic nitrogcn fixation, microorganisms

inu.riiea. fioctremisrry and genetics of nitrogen fixation, measurement ofniaogen

fixation, ecological and economic importance ofnitrogen fixation'

Unit IVMicrobial transformation of phosphorus in soil

Microbial transformationof sull'ur in soil

Microbial trnnslbrmationof iron in soilgi;ie.tilirc,l,, - bacterial fertiliz€rs and production olrhizobial inocularts and blue-

grcer algae. quality control tesrts

Prpcr lll llll353 Soil Ilicrobiolop (l'raeticxls)

Setting of WinogradskY's column

Istimition olsoil cornposition by sedimentation n)cthod

inumcralion ofsoil miiroorganisms (bacteria), iiclinomycctes, fungi) by standard plate

countlistimation ofsoil microbial activity by CO2 cvolution

lsoiation oicellutose decomposing microbes arrd cstimalion ofcellulose activity

EJn,"tion olanr-onifiers, nitrifiers and dcuitrilicrs in soil by MPN METHOD

l)l':l]lli ,lll'' "" " ''g or- Ilhiz"hitrnr \r'i rron, root nodules iucr Azospirilrun, lionr grasses

Biological onrichlrcnt isolation ofRhizohiLrm f.rorn soil bv Lconard Jar experimcnlNodulation tcsting b). lubc/jar nrelhodS,'lrrbiliu.rr!i,'rr ut r.rck phosphare h1 mr(ruorgirni:rn5 anJ e\rinrnrionI(sl )! ,ur rDir)('rII leachtng by I hrobrcilfus spA,,rs.rnclr for sullhatc reducing bact(n,1Ohicn-nrion rnd asscssment oIsorl algce.clgal biofcrtilizersBrctcriel hiottrtilrzcrs and lhcir useIlsri ation ofN2 Iixalion (Micro Kjcdhasl method,/Gc method)

Ilcconrntcnderl Ilooks

Soil Microbiology hy Alexander MartinN{icrrrbial r:cology, Iundar)tentals and Applications Ed. Benjarnin-CurrmingsSoil lliotcchnology hy JM IynchMicrobial Iicology: Organisnls, Habitats, Activities by Stolp, I LSorl Mit'robiolrrgy ,rnd Uiochemisrry by paul 1.. and pi. r-trrifMicrobi.ul L-colegl: l,rinciples, Methods and Applications b1, Lavin, Seidlcr, l{ogulBiological Nitrigcn Fixation by euispelSoil Microorganisrns and plant Grouh byN.S,. SLrbbaRao.I-aboratory experimcnts in microbiology ty Gopal Reddy et al

M.Sc, (Finat) III Scrnester MicrobiotogyI'aper lV i\tll30l Mcdical Bacteriotogy (l units r lS h r:s"= 60 hrs tcachingy

I nir I

Principlcs ,r I tr lcrlical iUicrobioology:Classiticxtion ol lllcdically important mioro-organisms. Nonnal Iora ofhun)an bodyOrigin ofnormll llom, rolc ofthc resiclent tloia, eflccr ofantirrri",,riri"i"g",ri.'", *.rrafi)ora, charactLtistics of normal floraaDistribution arld occurance ofnormal flora (Skin, conjuncliva, rrosc, nasoplrarynx,sinuscs. rrrourn. uppcr rcspiralory lracl. inlcsrinal tmct, rrrogeni,;i i;;.;;" " ,"Brctrri,r rrr thc bL,od rnd tissrrcs. firclors sinl]ucncing norm'al fl,,ra.

Unit ItPIope|tir'i ulpirtlrogcnic microorga isms. lactors thal inlluence pathocenicitvIypr niinti.riorrs. st,rrrce ofinlccrions. d ifle."nr n.od".,r"rn. jf ;;i...:;i";;'

Disgrrostie microbiology Types ofspecimen. spccinrcn collection, transportation ofspccitncn. f,rocersing. laboratory inrcstrgations- spcciilc lab I ests, non-spccific lab tests.diognosis nnd rcportUsc of lab animals in diagnosric microbiodlcrgl

Unil lllSystcmaric baotcriology - Detailed sludl-. of morphology, cullural characteristics,iu)lillcnic :rruclurc, pathogenesis. diagnostic lab tcst. epidcmiology, prevention and

trdatmcnt ol rhc lbllowing bacterial pathogens.Btcterial eir bornc inlections Il-Ilacmolytic strcpk)cocco, pncumococci,(:orynchddci fi diphlheriae, Mycobacterium tihertulo.tis, Mycohac erium leproe,Neis.tl,titt n.ni giliLlcs, haemophilus inJluenzde-

Scxually t.rrrsn)iltcd diseascs caused by bacletia, Ircponint pullidum, Neisseria

Unit IVSvsstcrnirtic irrcleriol9l)gy Detailed study ofnx,rfholoqy, cullural characteristics,arliscnic \tnrctlrre, pathogenesis, diagnostic lab lcsts. cpidcmiology, prevcntion andlreatment ol lhe following pathogenic bacteriVatcr bornc inl'ections - E-coli. Salmonella typhi, Shiy;:lla lvsenteriae,s Vibriocholcr.roc- Wol]nd infections Slaphvlococcus aurL'u.r, ( losticliu),m tetani, Clostridiumwelchi. l'strnktmonas.

I'apcr IV IIB 35,1 Mcdical Ihctcriology (Practicals)

Preparatiou ofdiffereqt types of cultue media/observation. Mannitol salt aga., BlairParker medium, Macconkey agar, Lowensten-Jension medium, Wilson Blair Bismuthsulphitt' mrdirrm. Biochemical media.Staininq lechniques - Gram's staining, AFB staining, Albcrt Slaining. Capsular stainingIsolrrtion lntl identilication ofvarious pathogcnic hrclerix hv nricroscopic. biochcmical,cn,.irnir(ic r.rl s.rol,)gicll tests

llirtlcr i,,[, ri,:,rl r':<irnrinatiorl of urin.. pus. throlt s\\,ll) eiL, lior]r pxli.nts tbr diaqnosis

Iirtorrtnterrdcd llooks

Rc!ie,,v oI \lcLlical llicrlobiology by Ja\.iilz, l1clni(k :rn(l .^d.lbcrqI)iagnostic )"licrobiology by Bailey and ScottiVledical i\licnrbiology by Cruckshanak ct al Vol I & II'Icxt book ol l!,licrobiology by Ananthanarayanan and jiryaram Panikerllactcrirrl discuscs by Wilson aod Topleylvf edical i\'licrobiology by Slre(ies[,aboratury cxpcrtncnts in microbiology by Gopnl licddy et al

M.Sc. (Final, IV \rnrertcr MicrohintnnPaper I MB {0t Motccutar Biorrchnoto$ t{ unit\ r ii"t'is = oO tr.. te"ctrinel

Unit ICcne regulation and exprcsrion IR.p..,;:;;;;:.,,:;;;:';';;..1,:.i'fil:".,1I1i,1;l::ill,l;l:.phane operons

L'ene retulation in euka|rotic srsr.:

:ll";;;;;lr ;:;" ,;:i:

_,.Til,ll .i$lli

t,Hi:,ffi " ?.il::5::|":: j;:.,*,:ess:lCer. !AMP. cUMP. prorcin kin rsr,. ( j-nr,,r(ins5tero/d/n(plide hormorre regulution. tr..rrc,pcrrlic regulation

Urit IIPlasmid.s Dcfinirion..rlr'(s oi-nr.rsrnids. idcntification and crassitication oi.piasmids,purtrcarron ofpla5mids. olrsmid rr.lnsfar ar.'l irr.n,..tuni...-iio"ii".i.i"iion l, ounrf",Transposable elemcnrs befinirr.n. clcrccri,,

"t ,..r;;.irl;; ;;;"i*ir, ,1p.. rt.bacteriat kansposons..""tunir'n. ulr.u,,.po.i;i";:,;;;_;t;;;;. lr'r'ilT,lr* ",hansposons.

PCR.TechnologS Principlc. dc.igning nfrrirncr. p(.R n)clhodoto].:\. RT-p(.R,mLrltiplex PCR. idenfiricerron rrr nr n!-j,,-,..,,s,iri*,;li;'ffi;l[iJ.il:;.r,Unit IIICeneticc.rgineering ( lunrng vrctor.. enzrntc\ rn\r\l\cd in genctic entr.rnecring./ f\lrerctron mapping. clnning .1s,sqr;... rncrhnJ. r,t genr rran-.f* - '

-uelcct,on ol clones and lheir ernression Exprcsstoi ofcloncd gcnes in .veast aid E.coli,Blotrnat\sis Sourhem. Northcrn cna n,"rr.rns tit,rii- iino ,r""iriil',.., ox"h1'bridizarion.

S:lo.Trc and c.-D\A tibrary consrrucrion und a;,ptic.rrion.

Hybndoma lechnoloSr - principle ond appliccri,n

Unit IV

X^1:"{,1 ll*"1' Rl.Lp. RAPD. ArLp. t6s r.RNA rninsuene ctup and microarays_ Assa\s. rpplications in disea,ie p;files. drug rargel, genediscovery, drug action and toxicin,Molecula. biology dara bases and bioinlbnnarics Ovcrvicw and dala mining ofsequence dala bases. DNA sequencing mcthodologl rnd snfi *".". ."uu"n".companson and alignmcnr. Conccnt i, f fhurmu. ogi:nom ic. . rn,l i,.

"nnii.rii",,IPR and patenting - Concept and overr rcs

Paper I MB45l Molccutxrlliotcchnology (l,racticats)

Rest clion mappingPCR technique - demonstrationPlasmid detectioll and Isolation of plasmid DNAPreparation of competent cells and transft nrnationGene cloning in bacleria - denmnslration

Southem transler demonstralionDemonstration of ItFLp/AF LpInduction ofenzymes I_ac opcronfransposon mutagenesisDialysis and separation of proteins b). column chromatographv

Recommendcd Books

Nlolecular biolog).by David and lrcjii,ldcrMicrobial geoetics by Drvid and FrejlelderGenetics ofbacteria and thcir viruscs by Williffn ltaycsMolecular biolog) ofthee gene hy \\'rlsun et rlThe Biochemistry of nuclcic acrds h1 DJvro\on JNMolecular bioleclulology b) primeroseMolecxlar Biotechnology by Bcmard R. Glick and Jack J I,astern.*DNA Microarral,s Ed. M. Schena

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_''fermentative production ofglutanric.rciJ - II\cs. 1l,cr,nr!anism. ino'culunr prcparatron.production mediurn. fcrment3lirrn Jnd doun,,r"u. pr,r.."aii,,g

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F'ermentative producIion oItetracy.clines, uses, chlortetracycline- oxt._retracycline,Ielracvcline and semisynthetic tel;acyclines. slructures, microorBanisrns. inoculumprenarariL,n. production rncdium. lermentarinn ond re.o,ren. m.tiods

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!l::1|.0.11"rt,:lT" l.,ns -Subs(rules. rypical srructures. microorganisms. inmoc;utumpreparatron. I I -hldroxy lation. process and reco\ cD.

PrincipJcs of raccineproduction end lypes oI\accine::Mic[objal biopcsticidesMicrobial products fiom Elcnelically modified (cloned) organisms eg. Insrulin

Paperll MB4S2 Microbial Biotechnolos/ (practicals)

Production ofethanol by flask fercnentatjon, recoverv ofethanol by distillarion andralcul.rtion oI tlrnrenut iL,n cifi ci(nc\Preparation of Beer by Microbial lermentationPreparation ol liom grcpes.liuits bv l.ennc lationProducrioll ofcitric acid b) lungcl t!rmenl.rrion. rcco\en and cstimalio,,l'roduclron o, amino rcid (Clut3mii acld lysiner b1 ferminrarionProductiot) ofamylase b1 lcrmcntatron. recovedn dnd estimationProduction and estimalioll ol penicrllin b] llask 6rmentationlmmobtllzed bacteria.4.ea5L cnzyme in lcrmcnrationScale up of fcmrcntation.

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(.1 units n l5 hrs = 6{) hrs tcaching)

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vpes of micr oorganisurs in milk. significance of microotga.nismsin milk. microbni0ological examinalion ofrnilk. conrrol ofmicrobial flora of milk

Unit IIMicrobes and animal interactions - I{un1en nti(.robiologv. tcrmilc miclobial conlmunitiesMicrobes in the prodrrction ofenerg.- frorn agricultural and domestic uastesMethanogenesis (Biogas) and microbiology of nrethogcnesis

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Utrit IVMicroorganisms and water pollutionWater-borne pathogeDic microorganisms and their transmissionSanitary quality ofwater. Waler pollution duc to degradation oforganic matterAerobic se\r'age treatment Oxidation ponds. lrickling lllters. a.tivatcd sludge treatmcntAnaerobic seu,age treatment Septic tonkMicroorganisms in air and their importance (hriel account)

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Unit IIIDetailed study ofcontac! and sexualiy lransmitled \ iral discases - Small pox. llerpesrHerper simplcx r irus t. i.lepatitis r iruse. hnd lhelr dis(ises/\cqulrcJ tmmunodeficiencr s\ ndrome (.\lDS )

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lV Ml| 454 N{edical Virolog, and parasitology (practicats)

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