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PREDICTIVE TOOLS FOR TESTING
NOVEL THERAPEUTICS
Human Immune System Engrafted Mice
TACONIC BIOSCIENCESHUMAN IMMUNE SYSTEM ENGRAFTED MICE
TO ORDERUS: 1-888-822-6642 | EU: +45 70 23 04 05 | [email protected] Human Immune System Engrafted Mice
Human Immune System Engrafted MiceThe super immunodeficient CIEA NOG mouse® is the ideal model for
engraftment of human hematopoetic stem cells, and therefore the
model of choice for immune system engraftment.
NOG mice reconstituted with a variety
of human tissues represent a valuable
tool for basic research involving the
human immune system. Engrafted
NOG mice enable efficacy testing
of immunotherapies as well as the
unprecedented ability to study tumor
specific immune cell activation.
Taconic offers study ready cohorts
of hematopoietic stem cell engrafted
NOG mice, as well as expert scientific
consultation on use of the CIEA
NOG mouse® for engraftment and
reconstitution with human tissues.
For additional information on
these models, visit Taconic.com/
human-immune-system-
engrafted-mice
APPLICATIONS FOR IMMUNE SYSTEM ENGRAFTED MICE
• Hematopoiesis and immune cell
development
• Infectious disease and human-specific
pathogens
• Efficacy testing of immunotherapy and
checkpoint blocking antibodies
• Safety assessment for new
therapeutics
• Transplantation and Graft vs Host
Disease (GvHD)
• Vaccine development
• Inflammation and allergy
• Autoimmune disease
CIEA NOG mouse®
IMMUNOTHERAPY / VACCINE
INFLAMMATION / ALLERGY
GvHD / TRANSPLANTATION
AUTOIMMUNE DISEASE
IMMUNOTHERAPY / VACCINE
SAFETY ASSESSMENT
HEMATOPOIESIS
INFECTIOUS DISEASE
+ human hematopoietic stem cells (huNOG)
+ human peripheral blood mononuclear cells (huPBMC-NOG)
+ human liver, thymus and hematopoietic stem cells (BLT-NOG)
DISCUSS YOUR NEEDSUS: 1-888-822-6642 | EU: +45 70 23 04 05 | [email protected]
TACONIC BIOSCIENCESHUMAN IMMUNE SYSTEM ENGRAFTED MICE
Human Immune System Engrafted Mice
TACONIC OFFERS THREE IMMUNE SYSTEM ENGRAFTED MODELS
huPBMC-NOG
NOG mice engrafted with human peripheral blood
mononuclear cells (PBMCs)
• Model for investigation of adult/mature
cell populations
• Use is limited to short term studies
• GvHD response can be used as a screening system
for T cell modulation drugs
• Available with normal or patient
derived PBMCs
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Human CD45+ cells were enumerated by flow cytometry at daily intervals following PBMC engraftment.Significant engraftment was observed after 14 days. The majority of human CD45+ cells are CD3+ T cells.
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PBMC PBMC+ anti-TNFαAntibody
Sham0 Gray0 Gray1.5 Gray2 Gray
Irradiation exacerbates GvHD in PBMC engrafted NOG mice. Survival curves for PBMC engrafted NOG mice at increasing doses of radiation with or without pre-treatment with anti-TNFα blocking antibody.
huNOG
NOG mice engrafted with human CD34+ hematopoietic
stem cells (HSCs)
• Stable engraftment of multiple cell lineages by 12-16
weeks post-injection
• Only mice with ≥25% hCD45+ in peripheral blood
are delivered
• Long term studies possible
huNOG are available off the shelf! Place your order now for immediate delivery.
BLT-NOG
NOG mice injected with CD34+ hematopoietic stem
cells (HSCs) and surgically engrafted with donor-
matched thymus and liver sections
• Stable engraftment of multiple cell lineages, with
enhanced T and B cell function
• Particularly suited to vaccine studies, antibody
generation studies and any study for modeling
human B cell responses
Human fetal thymus
Kidneycapsule
CD34* human HSCs
Human liver
Flow cytometry analysis of human cell lineages after CD34+ HSC engraftment. (A) Representative flow cytometry plot of the human (hCD45+) cell to mouse cell (mCD45+) ratio at 12 weeks post-engraftment. (B) Relative percentage of human cells expressing the B cell marker CD20 vs. the T cell marker CD3. (C) Consistent engraftment ratios of greater than 25% hCD45+ cells in over 80 percent of animals (n=91), with highly reproducible relative T cell and B cell enrichment levels. Each dot represents an individual mouse analyzed by flow cytometry 12 weeks post CD34+ HSC engraftment.
huPBMC-NOGhuNOGBLT-NOG
Health designation
Produced according to
Taconic’s commercial
health standards
Transportation
huNOG and BLT-NOG
may be transported
via Taconic’s regular
transportation network,
including on Taconic
truck routes
Licensing
No MTA or license fee
is required
100
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20
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Peri
phera
l Blo
od
(%
)
hCD45+
CD20+
B cells
CD3+ T
cells
CD4+ T
cells
CD8+ T
cells
hCD8
hC
D4
53
44
hCD3
hC
D20
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mC
D4
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hCD45
HUMAN IMMUNE SYSTEM ENGRAFTED MICE
CHOOSE TACONIC
For more than 60 years, Taconic has anticipated
the needs of the scientific community to deliver
models and services that meet the diverse needs of
biomedical and biopharmaceutical researchers.
Today that forward thinking and commitment to
working collaboratively has resulted in a client-centric
environment infused with a knowledge bank that
allows you to select the optimum model for your
study based on informed insight into the generation
of genetically engineered mouse and rat models.
YOUR COLLABORATIVE PARTNER
As a full-service biosciences company, Taconic can help
you acquire, test, develop, breed, cryopreserve, prepare,
and distribute highly relevant research lines worldwide.
Whether you require custom genetically engineered,
cell or tissue engrafted models or traditional models,
Taconic’s scientists will partner with you to rapidly
and efficiently deliver the highest quality models.
TALK TO A SCIENTIST
Our scientific teams are happy to meet and talk
with you about the most efficient way to achieve
your study goals. Working in partnership with
clients the world over, our scientific teams offer
expert advice that can help you speed up your
research and reduce your overall costs.
TALK TO A REPRESENTATIVE
For general information, you can talk to a member
of our customer service team. Our customer
service team is here to help you make the right
decisions and get the models you need fast.
Contact us at [email protected]
VISIT TACONIC.COM
For more information on the entire Taconic
portfolio of products and services designed to
help further your research, visit Taconic.com
TACONIC.COM
US: 1-888-822-6642EU: +45 70 23 04 05 [email protected]
Take Your Research Further
GEMS MANAGEMENT
Taconic’s fully integrated GEMs
Management brings innovative models
from design to study-ready cohorts with
unprecedented speed and transparency.
• Embryology
• Rapid Colony Expansion
• Contract Breeding
• Surgical Services
• Tissue Collection
• Genotyping and Molecular Analysis
• Microbiome and Germ-Free Research
Models and Services
GEMS DESIGN
Taconic Biosciences GEMs Design
empowers our clients to develop research
models specifically suited to the unique
needs of their discovery and development
studies or therapeutic programs.
• Gene Inactivation
• Gene Mutation or Replacement
• CRISPR Gene Editing
• Transgene Expression
• miRNA Expression
• Cohort Production Packages
PRECISION RESEARCH MODELS
Research organizations demand
precision tools that better reflect human
physiology. Taconic Biosciences leads
the field delivering innovative solutions
to meet these continually evolving
needs. Our core competencies include
the delivery of complex strategies that
both integrate human genetic sequences
and engraft human cells and tissues into
custom mouse and rat models.
• Human Gene Replacement
• Human Cell and Tissue Engraftment
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