howard rines 1 , hedera porter 1 , marty carson 2 , and jerry ochocki 2
DESCRIPTION
Introgression of Oat Crown Rust Resistance from Diploid Avena strigosa and Tetraploid Avena murphyi. Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2 1 USDA-ARS Plant Science Research, and Dept. of Agronomy and Plant Genetics, Univ. of Minnesota - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/1.jpg)
Howard Rines1, Hedera Porter1,
Marty Carson2, and Jerry Ochocki2
1USDA-ARS Plant Science Research, and Dept. of Agronomy and Plant Genetics, Univ. of Minnesota
2USDA-ARS Cereal Disease Lab, and Dept. of Plant Pathology, Univ. of Minnesota
Introgression of Oat Crown Rust Resistance from Diploid Avena strigosa
and Tetraploid Avena murphyi
![Page 2: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/2.jpg)
Crown Rust on Oat
![Page 3: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/3.jpg)
Crown rust of oat
ResistancePast – major genes from A. sterilis (6x),
relatively short-lived effectiveness
![Page 4: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/4.jpg)
Crown rust of oat
Resistance
Past – major genes from A. sterilis (6x), relatively short-lived effectiveness
Current – Pc94 from A. strigosa (2x), only effective single gene
![Page 5: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/5.jpg)
Crown rust of oat
Resistance
Past – major genes from A. sterilis (6x), relatively short-lived effectiveness
Current – Pc94 from A. strigosa (2x), only effective single gene
Alternatives –Major gene combinationsRace non-specific – partial, adult
plant, multigenic
![Page 6: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/6.jpg)
Crown rust of oatResistance
Past – major genes from A. sterilis (6x), relatively short-lived effectiveness
Current – Pc94 from A. strigosa (2x), only effective single gene
Alternatives – Major gene combinations Race non-specific – partial, adult
plant, multigenic
New sources – A. strigosa (2x) – CI 6954SP A. murphyi (4x) – P12
![Page 7: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/7.jpg)
Parents Rust susceptibilityA. strigosa CI 6954 HRA. murphyi P12 HRA. sativa cv. Ogle SA. sativa cv. Black Mesdag (BM) S
Rust reaction of the parental lines.
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Cross 1
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A. strigosa CI6954SP X A. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S
F1
(2n=1x + 3x=28) S
![Page 10: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/10.jpg)
A. strigosa CI6954SP X A. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S
F1
(2n=1x + 3x=28) S
colchicine fertile sectors self-pollinate C1
(2n=2x + 6x=56) S
![Page 11: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/11.jpg)
A. strigosa CI6954SP X A. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S
F1
(2n=1x + 3x=28) S
colchicine fertile sectors self-pollinate C1
(2n=2x + 6x=56) X A. sativa cv. Ogle S (2n=6x=42)
S BC1F1
partial fertile self, (1x + 3x + 3x) highly fertile female (2n=7x=49)
S
![Page 12: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/12.jpg)
A. strigosa CI6954SP X A. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S
F1
(2n=1x + 3x=28) S
colchicine fertile sectors self-pollinate C1
(2n=2x + 6x=56) X A. sativa cv. Ogle S (2n=6x=42)
S BC1F1
partial fertile self, (1x + 3x + 3x) X A. sativa cv. Oglehighly fertile female (2n=7x=49) (2n=6x=42)
S S BC2F1
(2n=6x + 0-7) 18R, 62S
![Page 13: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/13.jpg)
A. strigosa CI6954SP X A. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S
F1
(2n=1x + 3x=28) S
colchicine fertile sectors self-pollinate C1
(2n=2x + 6x=56) X A. sativa cv. Ogle S (2n=6x=42)
S BC1F1
partial fertile self, (1x + 3x + 3x) X A. sativa cv. Oglehighly fertile female (2n=7x=49) (2n=6x=42)
S S BC2F1
BC1F2 (2n=6x + 0-7)
(2n=6x + 0-7) 18R, 62S 27R, 130S
X
![Page 14: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/14.jpg)
A. strigosa CI6954SP X A. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S
F1
(2n=1x + 3x=28) S
colchicine fertile sectors self-pollinate C1
(2n=2x + 6x=56) X A. sativa cv. Ogle S (2n=6x=42)
S BC1F1
partial fertile self, (1x + 3x + 3x) X A. sativa cv. Oglehighly fertile female (2n=7x=49) (2n=6x=42)
S S BC2F1 X A. sativa cv. Ogle
BC1F2 (2n=6x + 0-7) (2n=6x=42)
(2n=6x + 0-7) 18R, 62S S 27R, 130S BC3F1
(2n=6x + 0–7) 157R, 170S
X
![Page 15: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/15.jpg)
Possible interpretations
• Suppressor in Black Mesdag– segregates out in BC1F2, BC2F1, and BC3F1 progeny
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A. strigosa CI6954SP X A. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S
F1
(2n=1x + 3x=28) S
colchicine fertile sectors self-pollinate C1
(2n=2x + 6x=56) X A. sativa cv. Ogle S (2n=6x=42)
S BC1F1
partial fertile self, (1x + 3x + 3x) X A. sativa cv. Oglehighly fertile female (2n=7x=49) (2n=6x=42)
S S BC2F1
BC1F2 (2n=6x + 0-7)
(2n=6x + 0-7) 18R, 62S 27R, 130S
X
![Page 17: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/17.jpg)
Possible interpretations
• Suppressor in Black Mesdag– segregates out in BC1F2, BC2F1, and BC3F1 progeny
• Precedent:– Pc62 (from A. sterilis) suppressed by Pc38– Pc94 (from A. strigosa) suppressed by Pc38
![Page 18: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/18.jpg)
Possible interpretations
• Suppressor in Black Mesdag– segregates out in BC1F2, BC2F1, and BC3F1 progeny
• Precedent:– Pc62 (from A. sterilis) suppressed by Pc38– Pc94 (from A. strigosa) suppressed by Pc38
• Tested:– Pc94 x Black Mesdag --- R
![Page 19: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/19.jpg)
Possible interpretations
• Suppressor in Black Mesdag
– segregates out in BC1F2, BC2F1, and BC3F1 progeny
• Precedent:– Pc62 (from A. sterilis) suppressed by Pc38– Pc94 (from A. strigosa) suppressed by Pc38
• Tested:– Pc94 x Black Mesdag --- R
• Tested:– New R x Black Mesdag– New R x Pc38
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Pc 38 Jerry Black(Pc 38) Mesdag
R S R S R S - - - - * 0 6 0 7 * 0 2 - - * 0 10 - - * 0 2 0 6 * 0 7 0 5 *
Pc 94 0 4 0 11 R,MR
CrossCrossOgle-c
R S 7 08 1 6 3 3 7 7 0 8 0
Plant No. Self
R S12(3) - 1 - -12(3) - 3 10 020(10) - 1 - -20(10) - 3 9 1 24(3) - 1 7 024(3) - 2 9 0
Inoculated at seedling stage with crown rust.
Test cross of resistant A. strigosa/Black Mesdag BC2F2
*crosses with Black Mesdag 35R 47S 2MR/R 3RSP
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Transmission of Introduced Resistance
• Past efforts with A. strigosa sources
1. Forsberg and co-workers– Resistance present on an alien addition or
substitution chromosome, only female transmission– Used gamma irradiation treatment
2. Aung and Chong-- Resistance on an alien addition chromosome,
unstable transmission-- Induced non-homologous pairing using crosses to A.
longiglumis CW57
![Page 22: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/22.jpg)
Transmission of Introduced Resistance
• Past efforts with A. strigosa sources
1. Forsberg and co-workers– Resistance present on an alien addition or
substitution chromosome, only female transmission– Used gamma irradiation treatment
2. Aung and Chong-- Resistance on an alien addition chromosome,
unstable transmission-- Induced non-homologous pairing using crosses to A.
longiglumis CW57
![Page 23: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/23.jpg)
A. strigosa CI6954SP X A. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S
F1
(2n=1x + 3x=28) S
colchicine fertile sectors self-pollinate C1
(2n=2x + 6x=56) X A. sativa cv. Ogle S (2n=6x=42)
S BC1F1
partial fertile self, (1x + 3x + 3x) X A. sativa cv. Oglehighly fertile female (2n=7x=49) (2n=6x=42)
S S BC2F1 X A. sativa cv. Ogle
BC1F2 (2n=6x + 0-7) (2n=6x=42)
(2n=6x + 0-7) 18R, 62S S 27R, 130S BC3F1
(2n=6x + 0–7) 157R, 170S
X
![Page 24: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/24.jpg)
Reciprocal crosses to Ogle-C of resistant A. strigosa CI 6954SP BC3 derivatives as:
BC3 plant female male _ S R MR S R MR
12(1)-3 4 3 0 1 1 012(3)-12 4 6 0 6 4 015(6)-8 3 6 7 2 1 520(5)-19 5 3 0 2 4 020(7)-6 4 7 1 4 3 120(7)-16 7 5 2 5 4 120(10)-7 3 2 0 5 8 020(10)-17 3 6 1 1 3 020(10)-20 5 5 0 7 3 020(12)-1 2 2 6 3 7 020(12)-9 7 3 5 7 8 0
Total 47 48 22 43 46 7
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Buckthorn nursery test of resistant A. strigosa BC3F2
• Plant No. R S R/S• 12(1)-1 39 16 2.4• 12(1)-7 54 14 3.9• 12(3)-5 56 15 3.7• 15(6)-19 2 26 0.1• 18(5)-4 41 13 3.2• 18(5)-10 52 19 2.7• 20(7)-6 51 15 3.4• 21(6)-6 43 5 8.6• 23(4)-15 18 0 N/A
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Cross 2
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A. strigosa CI6954SP X A. murphyi P12 (2n=2x=14) (2n=4x=28) HR colchicine HR
C1
(2n=2x + 4x=42) MR
![Page 28: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/28.jpg)
A. strigosa CI6954SP X A. murphyi P12 (2n=2x=14) (2n=4x=28) HR colchicine HR
C1 X A. sativa cv. Ogle (2n=2x + 4x=42) (2n=6x=42)
MR S BC1F1
(2n=1x + 2x +3X=42) S
![Page 29: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/29.jpg)
A. strigosa CI6954SP X A. murphyi P12 (2n=2x=14) (2n=4x=28) HR colchicine HR
C1 X A. sativa cv. Ogle (2n=2x + 4x=42) (2n=6x=42)
MR S
BC1F1 X A. sativa cv. Ogle (2n=1x+2x+3X=42) (2n=6x=42)
S S
BC2F1 (2n=?)
R,MR,MS,S
![Page 30: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/30.jpg)
Summary
• Oat crown rust resistance in accessions of A. strigosa (2x) and A. murphyi (4x)
![Page 31: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/31.jpg)
Summary
• Oat crown rust resistance in accessions of A. strigosa (2x) and A. murphyi (4x)
• Resistance suppressed in early generation crosses with A. sativa, but resistant segregants in later generations
![Page 32: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/32.jpg)
Summary
• Oat crown rust resistance in accessions of A. strigosa (2x) and A. murphyi (4x)
• Resistance suppressed in early generation crosses with A. sativa, but resistant segregants in later generations
• Resistance is transmitted equally via male or female. In most cases, transmission appears stable.
![Page 33: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/33.jpg)
Summary
• Oat crown rust resistance in accessions of A. strigosa (2x) and A. murphyi (4x)
• Resistance suppressed in early generation crosses with A. sativa, but resistant segregants in later generations
• Resistance is transmitted equally via male or female. In most cases, transmission appears stable.
• Preliminary segregation data suggest most lines have a single major gene.
![Page 34: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/34.jpg)
Summary
• Oat crown rust resistance in accessions of A. strigosa (2x) and A. murphyi (4x)
• Resistance suppressed in early generation crosses with A. sativa, but resistant segregants in later generations
• Resistance is transmitted equally via male or female. In most cases, transmission appears stable.
• Preliminary segregation data suggest most lines have a single major gene.
• Hypersensitive response of many plants further support single major gene.
![Page 35: Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2](https://reader035.vdocuments.mx/reader035/viewer/2022062309/56814588550346895db26c3c/html5/thumbnails/35.jpg)
Cross 3
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A. sativa cv. Ogle X A. murphyi P12 (2n=6x=42) (2n=4x=28) S HR
F1
(2n=3x + 2x=35) MS, MR
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A. sativa cv. Ogle X A. murphyi P12 (2n=6x=42) (2n=4x=28) S HR
F1
(2n=3x + 2x=35) MS, MR
colchicine fertile sectors self-pollinate F2 C1
(?x + ?x) (6x + 4x) 2n = ? (2n=10x=70)
S S
X
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A. sativa cv. Ogle X A. murphyi P12 (2n=6x=42) (2n=4x=28) S HR
F1
(2n=3x + 2x=35) MS, MR
colchicine fertile sectors self-pollinate F2 C1
(?x + ?x) (6x + 4x) X A. sativa cv. Ogle 2n = ? (2n=10x=70) (2n=6x=42)
S S S BC1F1
(2n= 6x + 2x =56) S
X
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A. sativa cv. Ogle X A. murphyi P12 (2n=6x=42) (2n=4x=28) S HR
F1
(2n=3x + 2x=35) MS, MR
colchicine fertile sectors self-pollinate F2 C1
(?x + ?x) (6x + 4x) X A. sativa cv. Ogle 2n = ? (2n=10x=70) (2n=6x=42)
S S S BC1F1 X A. sativa cv. Ogle (2n= 6x + 2x =56) (2n=6x=42)
S S BC2F1
(2n=6x + 0-7) S
X
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A. sativa cv. Ogle X A. murphyi P12 (2n=6x=42) (2n=4x=28) S HR
F1
(2n=3x + 2x=35) MS, MR
colchicine fertile sectors self-pollinate F2 C1
(?x + ?x) (6x + 4x) X A. sativa cv. Ogle 2n = ? (2n=10x=70) (2n=6x=42)
S S S BC1F1 X A. sativa cv. Ogle (2n= 6x + 2x =56) (2n=6x=42)
S S BC1F2 BC2F1
(2n=6x + 2x=56) (2n=6x + 0-7) S, MS S
X
X
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