How well do your enzymes

work? L/O ;- To know what can affect

how well an enzyme works

What does this phrase say?

• (Beryllium – Boron) / Nitrogen / (Zinc – Nitrogen) / Yttrium / (Manganese – Nitrogen) / (Beryllium – Boron) / Sulphur

• (Cadmium – Carbon) / Iodine / Germanium / Sulphur / (Titanium – iodine)

• Yttrium / Oxygen / (Copper – Carbon) / (Krypton – Potassium)

• Fluorine / Oxygen / Oxygen / Cadmium – Carbon • Samarium / Aluminium / (Lithium – Iodine) / Erbium • (Maganese – Nitrogen) / Oxygen / (Lithium – Iodine) /

(Beryllium – Boron) / Carbon / (Copper – Carbon) / (Lithium – Iodine) / (Beryllium – Boron) / Sulphur

• Sulphur / Oxygen • (Titanium – Iodine) / Helium / Yttrium• Carbon / (Sodium – Nitrogen) / Nitrogen • Beryllium • (Sodium – Nitrogen) / Boron / Sulphur / Oxygen / (Krypton –

Potassium) / Beryllium / (Cadmium – Carbon)

Enzymes can only one on one type of food but else do you think may affect

how well they work?

Enzymes are affected by

Temperature and pH

What temperature do you think your

enzymes work best at?

Trypsin is an enzyme which breaks down protein. If Typsin is added to

milk it will break down the milk turning it from cloudy to see-

through

How could you use Trypsin to find out how temperature affects how well enzymes work

ProcedureProcedure• Take 3 boiling tubes, and fill them each with 10ml of milk – Take 3 boiling tubes, and fill them each with 10ml of milk –

place them in a rackplace them in a rack

• Add Add 10ml10ml of of cold Trypsincold Trypsin to to oneone boiling tube with milk in, and boiling tube with milk in, and begin the timer – time how long it takes for the solution to begin the timer – time how long it takes for the solution to become transparentbecome transparent

• Repeat this with the second boiling tube, but this time add Repeat this with the second boiling tube, but this time add 10ml warm Trypsin10ml warm Trypsin

• Repeat this with the third boiling tube, but this time add Repeat this with the third boiling tube, but this time add 10ml 10ml boiling Trypsinboiling Trypsin

• Record all your resultsRecord all your results

How will you know when it has gone see-

through?

Assessment• What does you experiment tell

you about enzymes?• Try to explain why this happened?•Explain how ACCURATE you think

your results were?• How could you make your results

more accurate?

TemperatuTemperaturere• This experiment This experiment

shows that shows that enzymes work enzymes work best at a certain best at a certain temperaturetemperature

• If it is too cold the If it is too cold the enzyme takes a enzyme takes a long time to break long time to break the food downthe food down

• But if it is too hot But if it is too hot the enzyme may the enzyme may not function at all not function at all – it has been – it has been denatureddenatured

• Enzymes in the human body usually denature (stop Enzymes in the human body usually denature (stop working) above 45working) above 45ooCC

QuizQuiz

1. Enzymes are made of protein.

2. Enzymes only work in acid solutions.

3. Enzymes can only break down molecules.

4. A protease is an enzyme that breaks down protein into smaller molecules.

8A Digestion - True or False?

True or False?

TRUE

FALSE

FALSE

TRUE

1. An enzyme works best at a particular temperature.

2. Most enzymes in the human body work best at about 57°C.

3. Enzymes work best in boiling water.

4. Snakes produce one enzyme that can digest everything in its victim from the skin to the bones.

5. Amylase is an enzyme that breaks down starch.

8A Digestion - True or False?

True or False?

TRUE

FALSE

FALSE

FALSE

TRUE