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PRESS & AWARDS

Dr. Gabriel Lee (gabriel@topsciedit.com)

1998 - Singapore O & A level certificate

2004 - B.S, Biochemistry,

U of Wisconsin-Madison

2011 - Chief research advisor at TopSCIedit.com

2012 - PhD, Biomedical Science,

U of Massachusetts Medical School

ABOUT MYSELF

! " " !

Dr. David Lambright 推薦函 1

University of

Massachusetts

Medical School

David Lambright

Professor

Program in Molecular Medicine

University of Massachusetts Medical School

373 Plantation Street

Worcester, MA 01605

508.856.6876 (Office) 508.856.4289 (Fax)

August 24, 2013

RE: Mengtse Lee

To whom it may concern:

It is pleasure to write in enthusiastic support of Dr. Mengtse Lee's application for a teaching or

research position. Mengtse completed his graduate thesis research in my lab at the UMASS

Medical School.

Mengtse's thesis research concerned GEFs (GDP/GTP exchange factors) and GAPs (GTPase

activating proteins) that control membrane trafficking by activating and deactivating GTPases of

the Arf and Rab families. His first project involved a collaboration with a postdoctoral fellow to

solve the crystal structure of the autoinhibited form of a Sec7 domain GEF that activates Arf

GTPases in response to stimulation of cell surface receptors (DiNitto, Delprato, Lee, et al., 2007,

Mol. Cell 28: 569-583). Mengtse subsequently became interested in Vps9 domain GEFs, which

activate Rab5 subfamily GTPases, and in particular, the possibility (suggested by structural

observations) that Vps9 and Sec7 domains GEFs evolved similar mechanisms to promote

nucleotide exchange even though their overall tertiary structures are unrelated. To explore this

hypothesis, he undertook a stopped flow kinetic comparison of both the intrinsic exchange

reaction for several Arf and Rab GTPases as well as the reactions catalyzed by Sec7 and Vps9

domains. These studies, which represent the first detailed kinetic analysis for either of these

important domain families, demonstrate a fundamental relationship between the catalytic

mechanisms, including very similar kinetic properties, linkage equilibria, and dependence on the

Mg2+ co-factor. A manuscript describing this work has been completed and will be submitted in

the near future. Here I should mention that Mengtse was the first person in my lab to use stop

flow and the first to do a detailed kinetic analysis. Although I provided critical feedback,

Mengtse was responsible for the design, execution, and analysis of these experiments.

In addition to the crystallographic and kinetic studies described above, Mengtse generated a

variety of reagents, including antibodies and fluorescent protein fusions, for cell based analyses

of Sec7 and Vps9 domains GEFs, TBC domain GAPs, and various Rab GTPases. He has

considerable experience with mammalian cell culture, western blotting, co-immune precipitation,

siRNA knockdowns, and fluorescence microscopy. Some of this work has been published

(DiNitto, Lee, et al., 2010, Biochemistry 49: 6083-6092) and we are also preparing a manuscript

on at set of TBC domain GAPs that will include subcellular localization analyses that Mengtse

performed with the help of Greg Pazour's lab in my department.

Mengtse's command of both spoken and written English is excellent. For example, he wrote a

complete draft of the paper comparing the catalytic mechanisms of Vps9 and Sec7 domains as

well as a review article on the structural mechanisms for regulation of trafficking by Rab GTPase

(Lee et al., 2009, Traffic 10:1377-1389). Like his thesis, the drafts were scholarly and generally

well written.

SCIENTIF IC PAPERS

What is a scientific paper?

1. Fixed number of words

2. Academic English

3. Fixed format

ACADEMIC ENGLISH

What is academic English?

Not spoken or conversational English

Serious, formal English

Usually written from the third person.

An example of conversational English:

Let me tell you that eukaroytic cells are

sorted into different network of

endomembranes.

C O N V E R S A T I O N A L E N G L I S H

Let me tell you that eukaroytic cells are sorted into a network of endomembranes.

First person, casual, wordy

Eukaryotic cells are compartmentalized into a network of endomembranes.

C O N V E R S A T I O N A L E N G L I S H (cont.)

I heard that journal editors like authors to

write in simple English. Journal editors

hate to see literary tricks(特殊寫作技巧)

and metaphors (暗喻).

Y O U R T U R N …

I heard that journal editors like authors to

write in simple English. Journal editors

hate to see literary tricks and metaphors.

Literary tricks and metaphors are not

seen in academic journals. Journal

editors are happy with plain English

vocabulary.

A N S W E R

J O U R N A L W R I T I N G F O R M A T

Abstract 摘要

Methods 研究方法

Results 結果

References 參考書目

Introduction

緒論

Discussion

討論

T H E I N T R O D U C T I O N

Why is the

introduction an

inverted ladder

shape?

Abstract 摘要

Methods 研究方法

Results 結果

References 參考書目

Introduction

緒論

Discussion

討論

T H E I N T R O D U C T I O N (CONT.)

Background (Big picture)

Literature review

Research rational

& hypothesis

Research value

Brief

summary of

results

Why is the

introduction an

inverted ladder

shape?

Bad Example!

There is not enough

background information

for the audience to

understand what is going

on.

G I V I N G C O N T E X T

This essay is now

readable.

Additional background

information provides

enough context for the

audience to understand

the content.

G I V I N G C O N T E X T (cont.)

1. In recent years, there has been a tremendous

wave of interest in the relationship between

and _______.

9 SENTENCES for the INTRODUCTION

Example: In recent years, there has been a

tremendous wave of interest in the relationship

between consumption of hydrogenated oil

(氫化油) and cancer.

2. One of the more intriguing issues prevailing

throughout recent years of research is the question

of how _______ causes _______.

Y O U R T U R N …

Example: One of the more intriguing issues prevailing

throughout recent years of cancer research is the

question of how consumption of hydrogenated oil

causes cancer.

2. One of the more intriguing issues prevailing

throughout recent years of research is the question

of how _______ causes _______.

A N S W E R

3. Recently, _______ is getting considerable

attention from _______.

Y O U R T U R N …

3. Recently, _______ is getting considerable

attention from _______.

Example: Recently, hydrogenated oil is getting

considerable attention from the cancer

research community.

A N S W E R

4. _______has increasingly been the object of

_______ research in recent years.

Y O U R T U R N …

4. _______has increasingly been the object of

_______ research in recent years.

Example: Hydrogenated oil has increasingly

been the object of cancer research in recent years.

A N S W E R

5. A number of issues have appeared on recent

research on the relationship between _______

and _______.

Y O U R T U R N …

Example: A number of issues have appeared on

recent research on the relationship between

hydrogenated oil and cancer.

5. A number of issues have appeared on recent

research on the relationship between _______

and _______.

A N S W E R

6. The literature is full of discussions surrounding the

relationship between _______ and _______.

Y O U R T U R N …

Example: The literature is full of discussions

surrounding the relationship between hydrogenated

oil and cancer..

6. The literature is full of discussions surrounding the

relationship between _______ and _______.

A N S W E R

7. For sometime there has been evidence that

_______caused _______.

Y O U R T U R N …

Example: For sometime there has been evidence

that hydrogenated oil caused cancer.

7. For sometime there has been evidence that

_______caused _______.

A N S W E R

8. A growing number of research studies are now

available to shed some light on how _______

caused _______.

Y O U R T U R N …

Example: A growing number of research studies

are now available to shed some light on how

hydrogenated oil caused cancer..

8. A growing number of research studies are now

available to shed some light on how _______

caused _______.

A N S W E R

8. With increasing usage of _______, it is crucial to

find out whether it is _______.

Y O U R T U R N …

Example: With increasing usage of hydrogenated

oil in snacks, it is crucial to find out whether it is

carcinogenic.

8. With increasing usage of _______, it is crucial to

find out whether it is _______.

A N S W E R