how are synaptic vesicles clustered?
DESCRIPTION
How are synaptic vesicles clustered?. Daniel Gitler, Department of Physiology and Cell biology, Faculty of Health Sciences and Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev. Presynaptic terminal. Postsynaptic terminal. Gitler et al., J Neurosci 2004. - PowerPoint PPT PresentationTRANSCRIPT
How are synaptic How are synaptic vesicles clustered? vesicles clustered?
Daniel Gitler, Department of Daniel Gitler, Department of Physiology and Cell biology, Physiology and Cell biology,
Faculty of Health Sciences and Faculty of Health Sciences and Zlotowski Center for Zlotowski Center for
Neuroscience, Ben-Gurion Neuroscience, Ben-Gurion University of the NegevUniversity of the Negev
Presynaptic terminalPresynaptic terminal
Postsynaptic Postsynaptic terminalterminal
Gitler et al., J Neurosci 2004
Vesicles redistribute into the axon when synapsins are ablated
500 nm
Orenbuch et al., J Neurosci 2012
0 10 20 30 400.0
0.2
0.4
0.6
1.0 SypI-EGFP
TKO
Flu
ores
cenc
e R
ecov
ery
Time (min)
WT
Measuring vesicle mobilityW
T
Before Bleach Recovery
Darcy at al. 2006
Orenbuch et al., J Neurosci 2012
Model for vesicle clustering
Synapsins are ATP binding proteins
Esser et al., 1998
Crystalographic structure of ATP bound to the central domain of synapsin I
Do mutations in ATP binding sites affect clustering and mobilization of vesicles?
Other topics
• Does mobilization of reserve vesicles require endocytosis?
• The basis for epilepsy in the synapsin TKO mice and in cases of human familial epilepsy linked to synapsin
• The possibility of a functional interaction between synapsin and alpha-synuclein, a protein involved in Parkinsons disease