Histology

Muscle Lab

Stephen Ernst, Ph.D. / Matt Velkey, Ph.D.

58 Skeletal Muscle

Longitudinal

Striations

Striated Muscle Fiber (cell)

Peripheral Flattened Nuclei

Endomysium

58L Skeletal Muscle

2 consecutive Z line =

Sarcomere

Dark A Band (myosin)Light I Bands(actin)

Divided by Z Line

Slide 58 Skeletal MuscleCross Section

Muscle Fibers

Peripheral Nuclei

Skeletal Muscle

Peripheral Nerve

Slide 59 H&E(Use this slide to examine CT layers in skeletal muscle; ignore, nerve fasicles which are also present)

Slide 59 Skeletal MuscleCross Section H&E Stain

Epimysium

Perimysium

Individual MusclesTendon

Epidermis Adipose TissueDermis

SKIN:

Epimysium

Perimysium

Endomysium

Slide 59 Trichrome Stain

Slide 71 (even)Slide 71 (even)

Muscle fibers (purple)Muscle fibers (purple)

Neuromuscular junctions

Neuromuscular junctions

AxonsAxons

Slide 71 (even)Slide 71 (even)

Neuromuscular Junctions(a.k.a. motor end plates)Neuromuscular Junctions(a.k.a. motor end plates)

Slide 71 (even)Slide 71 (even)

AxonAxon

Node of RanvierNode of Ranvier

Neuromuscular junctionNeuromuscular junction

Slide 71 (even)Slide 71 (even)

Neuromuscular junction(viewed from top)Neuromuscular junction(viewed from top)

AxonAxon

Slide 71 (even)Slide 71 (even)

Neuromuscular junction(viewed from top)Neuromuscular junction(viewed from top)

AxonAxon

Slide 71 (odd)Slide 71 (odd)

Neuromuscular spindleNeuromuscular spindle

Skeletal muscleSkeletal muscle

Slide 71 (odd)Slide 71 (odd)

Skeletal muscle (extrafusal fibers)Skeletal muscle (extrafusal fibers)

outer capsule of neuromuscular spindleouter capsule of neuromuscular spindle

Intrafusal fibersIntrafusal fibers

inner capsuleinner capsule

Slide 71 (odd)Slide 71 (odd)

Peripheral nerveNOT a neuromusclar spindle!

Peripheral nerveNOT a neuromusclar spindle!

Skeletal muscle(extrafusal fibers)Skeletal muscle

(extrafusal fibers)

57 Cardiac Muscle

IntercalatedDisk

(Note: Slide 98 is also quitegood for Intercalated disks) Longitudinal Fibers

Cross Sectioned Fibers

branching

Large Nucleicentrally placed

29 Smooth Muscle (Intestine)

Cross Section

Longitudinal Section

Loose Connective Tissue

Small,Fusiform (spindle shaped)FiberCentral Flattened Nucleus

250 Vagina Smooth Muscle

Cross Section Smooth Muscle

Fasicle

Longitudinal Smooth Muscle Fasicle

CT

CT

CT

250 High Power Smooth Muscle Fasciles

CTSmooth Muscle