Histology LaboratoriesMolecules to Systems

2003Compiled by

James D. Jamieson, MD/PhDThomas L. Lentz, MD

No part of this image collection may be distributed outside of the Yale University Intranet.

Alberts, B. et al. Molecular Biology of the Cell. 4th Edition, Garland Science, New York, 2002.Gartner, L. P. and Hiatt, J. L. Color Atlas of Histology, Williams & Wilkins,

Baltimore, 1994.Kerr, J. B. Atlas of Functional Histology. Mosby, London, 1999.Kessel, R. G. and Kardon, R. H. Tissues and Organs: a text-atlas of scanning

electron microscopy. W. H. Freeman, San Francisco, 1979.Lentz, T. L. Cell Fine Structure. W. B. Saunders, Philadelphia, 1971.Lodish, H. et al. Molecular Cell Biology. W. H. Freeman, New York, 2000.Mizoguti, H. Color Slide Atlas of Histology. Nihon Shashin Shinbunsha, Tokyo.Young, B. and Heath, J. W. Wheater’s Functional Histology. Churchill

Livingstone, Edinburgh, 2000.Micrographs taken by George Palade, Marilyn Farquhar, James D. Jamieson,

Nicolai Simionescu, Maya Simionescu, David Castle, Thomas L. Lentz.

Web Resourceshttp://info.med.yale.edu/webpath/webpath.htmCushing Library Educational Software/Cell Biology/Several Histology Resources

AcknowledgementsSources of Micrographs, Diagrams and Figures

Bone Laboratory

Adult Bone

Some Useful Terms

Compact bone = cortical bone

Trabecular bone = spongy = cancellous bone

Osteoid: Bone matrix not yet calcified.

Woven bone: immature bone with randomly arranged collagen fibers. Produced when osteoblasts rapidly produce osteoid as in fetal bone development; fracture repair (callus).

Lamellar bone: regular parallel bands of collagen. Woven bone eventually remodeled into lamellar bone. The normal form of bone in adults.

Compact bone

Trabecular bone

Cortical Bone

Volkmann’s canals

Haversian canalsLamellar bone

Haversian canal

Osteon: Lamellar Bone

Osteocytes

Canaliculi

Osteocytes in Lacunae in Lamellar Bone

Trabecular Bone

Trabecular Bone: XS Long Bone

Muscle

Trabecular Bone

Trabeculae

Marrow

Periosteum

Endosteum

Periosteum

Fibrous layer

Cellular layer

Compact bone

Osteoclasts

Bone Marrow

Endosteum

Articular Surface of Long Bone

Articular Surface of Long Bone

Cartilage

Bone

Collagen layer

Bone Formation

Intramembranous Ossification

(No cartilage model; bones of skull)

Intramembranous Ossification: Fetal Skull

OsteoclastsOsteoblasts

Osteoclasts

Osteoblasts

Intramembranous Ossification: Fetal Skull

Endochondral Ossification

(Ribs, long bones, vertebrae; cartilage model)

Endochondral Bone Formation

Fetal Finger

Epiphyseal growth plates (GP)

DiaphysisEpiphysis (E) Epiphysis

Epiphysis With Trabecular Bone

GP

E

Metaphysis

Growing Tibia

GP

E

Articular cartilage

M

D

GP

Bone

Calcified cartilage

Osteoid

Growing Tibia

Chondrocyte Growth Sequence

Resting

Proliferation

Hypertrophy

Maturation

Zone of calcifying cartilage

Development of Trabecular Bone

Calcified cartilage

BoneOsteoblasts; osteoid

Bone Remodeling

Activation Resorption Formation Sequence

Osteoclasts

Howship’s lacuna

Osteoblasts (OB)

Osteoid

Vessels in Resorption Canal

Osteocytes in new lamellar bone

Resorption Canal XS

OB

Osteoid

Some pathology to think about.

Mammary Carcinoma Metastases to Vertebra

Callus at Fracture Site

New Bone at Fracture Site

Osteoporosis with Vertebral Collapse

Osteogenic Sarcoma/Osteoid