hemoglobin determination

Download Hemoglobin determination

Post on 03-Jun-2015

13.658 views

Category:

Health & Medicine

1 download

Embed Size (px)

DESCRIPTION

by bond king,nitish shah,rahul dev

TRANSCRIPT

  • 1. HEMOGLOBINDETERMINATION BOND KING NITISH RAHUL DEV

2. I. COLORIMETRICMETHODA. Direct visual colorimetric Method Tall quist method Dares Hemoglobinometer Acid Hematin method Alkaline Hematin method 3. B. Photoelectric colorimetric method1. Oxyhemoglobin method Measures normal hemoglobin Used 0.007 N NH4OH or 0.1% Na2Co3 Read with the wavelength at 540 nm 4. 2. Cyanmethemoglobin (HiCN) Also known as hemiglobin cyanide orferrihemoglobin cyanide All forms of hemoglobin are measuredexcept sulfohemoglobin Uses Drabkins solution Potassium ferricyanide Potassium cyanide Dihydrogen potassium phospate Distilled water PH = 7.0-7.4( blood capacity ) Used sahli pipet= (0.02ml or 20 micro liter) 5. PROCEDURE Place 5ml of Drabkins reagent into atestube Get 0.02 ml of whole blood using sahli pipet Place the 0.02 ml of blood in to drabkinsreagent through rinsing it. Mix and let it stand for 10 minutes Read in a spectrophotometer at 540 nm. 6. II. Specific gravity method/Gravitationalmethod CUSO4 method Specific gravity of copper sulfate = 1.053with an hemoglobin equivalent of 12.5 gm% Mass blood Procedure Collect blood sample Drop a blood into the solution Observe the activity of the blood Within 12 seconds, describe how the dropof blood behaves. 7. Interpretation :- Maintain = equal to 1.053 = 12.5 gm% Sink = > 1.053 = >12.5 gm% Float = < 1.053 =

Recommended

View more >