Hematopoietic and Lymphatic System

Blood Parasites

Trypanosoma

• Trypanosoma cruzi

Causing chagase disease.

• Trypanosoma bruci

African sleeping disease.

morphological forms of Hemoflagellate

•

Trypanosoma gambianse brucii

• Vector Tse Tse fly.

• Trypomastigote stage in the blood stream.

• African sleeping disease.

Life Cycle of Trypanosoma brucei spp

Life

Cycle

of

Trypanosoma

brucei

spp

Trypanosoma cruzi

Vector: reduviid bugs.

Disease: American Chagas disease.

Trepomastigote stage in blood.

"pseudocysts" of Trypanosoma cruzi

• containing  the amastigote stage in

heart muscle

Life Cycle:

Plasmodium spp.

• P. falciparum.

• P. ovale.

• P. vivax.

• P.malariae

Plasmodium Falciparum

a. Multiple ring stage inside RBC.

Plasmodium Falciparum

• Cresent or banana shape gametocyte

Plasmodium spp.

• Gametocytes of • P. ovale.P. vivax.• P.malariae.• Round in shape• Male -->Micro

gamete --> diffuse.• Female --> macro

gamete --> condensed.

Plasmodium life cycle

Leishmaniasis

• Leishmaniasis is transmitted through the bite of female phlebotomine sandflies.

• Leishmania donovani (Visceral VL)

• Leishmania tropica (Cutaneous CL)

• Leishmania braziliensis ( Mucocutaneous leishmaniasis MCL)

Leishmania spp.

• Promastigotes stage in sand flies.

• Amastigote in the tissue.

Leishmania life cycle•

Bacteriology

Media and additives present in blood culture bottle

• Several media are used for blood culture bottles like.

• Trypticase soy broth. Thioglycolate media, Brain heart infusion.additives SPS (sodium poly anethel sulfonate)

Media used for Isolation

• Blood Media: most commonly used.

• Chocolate agar :

• Thioglycolate media: (enriched)

• MacConkey agar: Enterobacteria.

inoculation

• . And we do the culture by streaking method.

Swap method

take the swap under sterile condition, rotate it on the first quadrant of blood agar plate and replace it in a thioglycolate broth.

Streptococcus pyogenes

• Colonies of Streptococcus pyogenes on sheep blood agar.

• Notice: * Presence of b hemolysis around colonies

• * Enhanced hemolysis around stabbing sites

• * Sensitivity to bacitracin (Disk A)

Streptococcus pyogenes

• Pin point colony: (white or gray)

• Gram stain: G+ve, cocci, single chain.

• Catalase enzyme: differentiate between

Streptococcus –ve

Staphylococcus +ve

Staphylococcus aureus

• G-positive cocci in clusters,

• typical of Staphylococcus aureus

• Catalase test used to differentiate Staphylococci

• from Streptococci

• Coagulase test used to

differentiate S. aureus

• from other Staphylococcus spp.

• Mannitol salt agar: selective and differential for Staphylococcus spp.

• (Staphylococcus aureus manitol fermenteryellow)

Pseudomonas aeruginosa

• Greenish discoloration of media due to production of pyocyanin by Pseudomonas aeruginosa

Pseudomonas aeruginosa

• Results of oxidase test

E. coli

• G-negative bacilli,• typical of E. coli

• Colonies of E. coli on MacConkey

agar

• (Pink color indicates lactose

fermentation)

• IMViC reaction of E. coli:

• + + - -

KIA A/A

Proteus spp.

• Proteus - Members of the genus Proteus will swarm at certain intervals and produce a pattern of rings due to their motility.

Urease Test, H2S

• (Positive Urease Test)

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