hematology manual for the technician

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(Sickling Test) : , , . Hb S SCA SCT EDTA Heparin ACD Hb S

: Sickling solution (Solubility Method)1 :* :1- 2 Sickling solution phosphate buffer Dithionite reagent .2- 20 .3- .4- 10 .* :- ( Hb S ) .- .

Hb electrophoresis

* : Sickling solution (S) (Sickling solution). .

() :* :1- Sodium hydrosulfite .2- (Cover glass) .( ).3- ( ) (Cover glass) .4- 2 24 .5- 40* :- .- .* : (Cover glass) 24 (Cover glass) . . (Sodium dithionite) , .

ESR

: ESR Erythrocyte Sedimentation Rate . . ESR : . . :- 0 13 .- 0 20 .- 7 11 . ESR : Non Specific :- .- .- .- .- . :- :1. .2. .- :1. .2. . ESR : ESR Westergren 300 200 .1. ( ) .2. .3. .

Westergren VES-MATIC 20 .

VES-MATIC 20

Complete Blood Count :

1- .."RBC .. "Red Blood Cells* .Polycythemia* .. Anemia2- ..WBC.. White Blood Cells Leucocytosis Leucopenia3- ..Blood Platelets..PLT* Thrombocytosis* thrombocytopenia4- Hb:5- PCV .. Packed Cell Volume /Haematocrit .. HCT- , .- .- (MCV / MCHC).6- Mean Corpuscular Haemoglobin .. MCH:- MCH () .* MCH .7- .. Mean Corpuscular Haemoglobin concentration ..MCHC:- MCHC .- MCHC .* MCHC Hypochromia Iron deficiency anaemia.

8- Mean Cell Volume .. MCV:- MCV .- Microcyte :1- Iron deficiency anaemia.2- Thalassemia.- MCV .- Macrocyte Megaloblastic anaemia.- :1- .. Folate deficiency.2- B12 ..B12 deficiency.9- Mean Platelet Volume ..MPV10- Red Cell Distribution Width ..RDW11- Platelet Distribution Width ..PWD 13g/dl 12 13 MCV CCMH 3 : Microcytic hypochromic Iron deficiency anemiaMacrocytic VitB12 and Folate deficiencyNormocytic normochromic Hemolytic anemia

Peripheral blood smear

EDTA :1- Thin smear Thick smear Giemsa ( ) ... Thick smear , .2- ( Immunoassay ) rapid immunodiagnostic strip test : Optimal Rapid Malaria Test ) :1- 2 ( wells ) ( Conjugate - well ( Wash - well )2- ( Conjugate - well ) buffer ( Wash - well ) buffer 1 .3- 10 ( ) ( Conjugate - well ) , .4- ( Dipstick ) ( Conjugate - well ) 10 .5- ( Dipstick ) ( Wash - well ) 10 . :-- ______ ( )- _________ ( P. Vivax P. Malaria P.Ovalae )- 3 ________ ( P.Falciparum )3- PCR .

COAGULATION

PT +PTT

(BCT)(1) (Bleeding Time B.T) : () (B.T). : .(2) (Prothrombin Time PT : . (Extrinsic Pathway) . : (PT) 11 16 .(3) (Partial Thromboplastin Time PTT) : (Intrinsic Pathway). : (PTT) 25 35 .(4) (Thrombin Time T.T) : (Fibrinogen). : (Thrombin Time) 16 22 ( 1 : 31) 8 10 ( 1 : 11).(5) (Fibrinogen Concentration) : . : 160 440 / .(6) ((Fibrinogen / Fibrin Degradation Products - F.D.P : (Fibrin) (F.D.P) (F.D.P). : 250 500 / .(7) (Haemophilia A and B) :1- (VIII) A .2- (IX) B . . : .(8) : : (VII , XI , XII) .(9) (Natural Anticoagulants)() Activity of Protein S : (S) : (Liver Disease) . (Pregnancy) . (Warfarin) . (Oral Contraceptives) . (HIV) (DIC) . (K) . : (S) 70 123 % .() Activity of Protein C : (C) : (Oral Contraceptives) . (Liver Disease) . (Pregnancy) . (HIV) . (DIC) . : (C) 70 140 % .() 3 (Activity of Antithrombin III) : 3 (Antithrombin III) : : 3 (Antithrombin III) . 3 (Antithrombin III) . : (Liver Disease) (Renal Disease) . (Oral Contraceptive) . : (Spectrophotometer).(10) (Lupus Anticoagulant LA) : (Systemic Lupus Erythermatous SLE) . . . (PTT) .Haut du formulaire

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