MEDT 105HEMATOLOGY LAB

A. Pre-examination Phase

1. Requisition form2. Blood Collection Techniquesa. Venipunctureb. Capillary Puncture

3. Order of draw4. Inappropriate Sites for Blood Collection5. Problematic Situations6. Submission and Receipt of Specimen7. blood smear preparation (manual and automated)

a. thick and thin smear preparation b. peripheral blood smear preparation

B. Examination Phase

i. identification of mature and immature blood cellsii. PBS grading

iii. PBS reporting

8. complete blood count (manual and automated)a. red blood cell countb. white blood cell countc. haemoglobin determinationd. haematocrit determinatione. platelet countf. differential countg. rbc indices

i. MCVii. MCHC

iii. MCHiv. RDW

9. reticulocyte count10. erythrocyte sedimentation rate11. osmotic fragility test12. Sickle cell tests13. special hematology tests

C. Post-Examination Phase

14. Validating results15. Retesting specimens16. Referring results

17. Releasing reports18. Retention of Specimens and Documents

D. Quality Assurance

Requirements: Syringes (3 or 5 cc) ETS Capillary tube (heparinized or plain) Clay and paraffin wax (sealant) 1 box glass slides Cover slip Alcohol pads Micropore Cotton 10-20 used or washed plain test tubes (5 ml) Wbc pipet Rbc pipet Hemocytometer 1 yellow quiz notebook

Grading System

20% - laboratory activity20% - quizzes10% - long exam10% - attendance

References

Dela Calzada, G. J., Faundo, A. C., Orillaza, M. A., Liu, E. B. (2012). PCQACL’s Standardization and Harmonization of Complete Blood Count in the Philippines. Quezon City : C & E Publishing

Brown, B. A. (1993). Hematology Principles and Procedures Internet Sources