HRS-10

Biomimetic peptidecombined with a red clover extract-For stronger andthicker hair

HAIR GROWTH CYCLEThe importance of hair in our lives cannot be overstated. Whether men or women lose their hair, they lose much more than their natural, youthful appearance. People also lose their self-esteem and self-confidence associated with healthy looking hair. Hair loss also, called alopecia, is a common problem affecting both men and women.

Normal hair follicle is surrounded by healthy connective tissue and dermal papilla, and has minimal or absence of inflammation nor oxidative stress. Hair loss sufferers will most of the time have the hair growth cycle disrupted by an increase of dihydrotestosterone (DHT) hormone, loss of connective tissue integrity surrounding the hair follicle, and an increase of the inflammatory process and oxidative stress in the scalp. With age, the decrease number and size of hair follicles in the scalp will also lead to thinner hair and an increase in hair loss.

SYNERGISTIC ACTION OF A PEPTIDE AND RED CLOVER EXTRACTHRS-10 is a biomimetic peptide (acetyl tetrapeptide-3) combined with a red clover extract rich in Biochanin A. HRS-10 efficacy is based on the association and synergistic action of its two ingredients.

HRS-10 has a direct action on 5-a -reductase which modulates dihydrotestosterone (DHT) to help prevent hair miniaturization. It improves ECM proteins integrity in dermal papilla for a better hair anchoring and reduces damages caused by inflammation, providing fuller, thicker and healthier looking hair.

NORMAL HA IR GROWTH CYCLE

A LTERED HA IR GROWTH CYCLEe A N A GEN + a TELOGEN HAIR LOSS

> No hormonal dysfunction> ECM proteins integrity> No i mmation

> a DHT: Shorthens anagen phase Miniaturization of follicles> Loss of ECM proteins renewal Follicle size reduction and loss of hair anchoring> mmation Disruption of hair cycle

F OLLICLE

> Inhibition of 5-a-reductase, thus modulating the conversion of testosterone to DHT> ECM proteins renewal> Cytokine reduction

> Limits miniaturization of follicles> Better hair anchoring> Normal hair cycle

HRS-10 BENEFITS H A IR LOSS HRS-10 EFFICACY

INFLAMMATAA IO N REDUCT IO N

DTDD H MO DULA T ION

ECM R E NE W AL AND ANC HORINGPROTOO E INS STI MULATIOAA N

a A N A G EN e T ELOGEN a H A IR DENSI T Y

L IMITS FO L L IC L EMINIATURIAA ZATIOAA N

B ETTTT ER HAIR ANCHORING

H AIR LOOKS T HICKER

S HEATAA H

F OLLICLE MINIATAA URIZATAA ION

IMPROPER ANCHORIN G

H AIR T HINNIG

N O MORE FOLLICLE

HRS-10

TARGETTING THE CAUSES OF HAIR LOSS

Inflammation reductionIL-1a mimics the inflamation process in human fibroblasts and induces IL-8, a pro-inflamatory cytokine.

HRS-10 helps decrease pro-i mmatory cytokines with a synergistic action compared to red clover extract alone.

DHT modulation via 5- a -reductase activityDHT is formed by the action of 5- a -reductase on testosterone. DHT causes hair loss by shortening the growth cycle, producing progressively shorter and finer hair. This is the study of the capacity of Biochanin A to inhibit the 5-a reductase activity in comparison with a well known 5- a reductase inhibitor EGCG (epigallocatechin gallate a potent antioxidant found in tea).

Biochanin A inhibits 5- -reductase activity.

ECM renewal and anchoring improvementHair follicles size is determined by: - T he volume of its dermal papilla; - T he volume of the extracellular matrix.

Healthy dermal papilla will produce good ECM proteins such as collagen type III and anchoring fibers such as laminin and collagen VII which will favor a good hair anchoring in the bulb surrounding tissue. If improper ECM renewal, hair will eventually lack vigor and will thin. Cycle after cycle, the follicle becomes smaller and finally, miniaturized and fall.

Proline is an amino acid constituent of collagen, therefore it represents a good indication of newly synthesized collagen.

Acetyl tetrapeptide-3 stimulates collagen production for better ECM integrity leading to a better anchoring.

Untreated Acetyl tetrapeptide-30

2

4

6

8

10+35 %

OH

-Pro

line

(μ

g/m

L)

Control IL-1α IL-1α +DMS1μM

IL-1α +Red clover

0.5% 1%

IL-1α +HRS-10

0.5% 1%

050

100150

20025030035 0400450 -17% -11% -20% -33% -48%

IL-8

(pg/

mL)

IL - 8 PRODUCTION BY FIBROBL A STS

Control EGCG 100μM

TY

PE

1

TY

PE

2

TY

PE

1

TY

PE

2

Biochanin A 100μM0

25

50

75

100+17,2% -93 %-64%-5 %-11%

5-α

redu

ctas

e ac

tivity

(%)

INHIBITION OF 5-a - REDUCTA SE

H YDROXYPROLINE DOSA GE IN FIBROBLA STS

Quantification of laminin, type III collagen synthesis induced by Acetyl tetrapeptide-3 on fibroblasts. It has been demonstrated that molecules which have an activity on these ECM proteins stimulation, also increase hair follicle size and improve hair anchoring.

Acetyl tetrapeptide-3 stimulates dermal papilla extracellular matrix proteins, and thus has a direct effect on structures surrounding hair follicles.

Collagen VII is a major constituent of the anchoring fibrils and is located in the basement membrane (DEJ) around the papilla. In this experiment, corticoids are used to induce a decrease in the hair papilla cells growth and ECM protein.

Acetyl tetrapeptide-3 provides a repairing effect at the dermal-epidermal junction level, helping hair anchoring.

IMPROVING HAIR FOLLICLE LENGTHHuman hair follicles in anagen phase are isolated from skin biopsys and cultured with or without Acetyl tetrapeptide-3 for 8 days.

Acetyl tetrapeptide-3 improves by 35% the length in comparison with untreated areas after 8 days of treatment.

Control(untreated)

+ Corticoïds Corticoïds+ Acetyl

tetrapeptide-3

0

1

2

3

4

70 %

Scor

e

Q UA NTIT ATIVE EV A LU ATION OF COLL A GEN VII

Type III Collagen Laminin0

20

40

60

80+285 %

+65 %

Fluo

resc

ence

inte

nsity

Untreated Acetyl tetrapeptide-3

S TIMUL ATION OF ECM PROTEINS SYNTHESIS BY FIBROBL A STS

Day 0 Day 80

500

1000

1500

2000

2500

3000

3500

4000+35 %

Folli

cle

elon

gatio

n (μ

m)

Untreated Acetyl tetrapeptide-3 (0.0006% HRS-10)

ME A SUREMENT OF HA IR LENGTH

Control + Acetyl tetrapeptide-3

Type III Collagen

Laminin

F IBROBL A STS

Skin + Corticoïds

Normal skin (untreated)

Skin + Corticoïds + Acetyl tetrapeptide-3

Collagen VII

DEJDermis

SC

Epidermis

PROVIDING FULLER AND THICKER LOOKING HAIR Test protocol

- 30 men with androgenetic alopecia: 15 treated with the HRS-10 lotion (5%) and 15 with a placebo.

- Daily application of 20 drops of the HRS-10 lotion or placebo for a period of 4 months.- A digital trichogram (TrichoScan professional) was taken at D0 and 4 months later.

HRS-10 induces a visible increase in the anagen hair density in comparison with placebo.

HRS-10 induces a strong reduction in the telogen hair density in comparison with placebo.

A/T ratio = Comparison of the number of anagen and telogen hair, which is an indication of the percentage of active hair follicles.

HRS-10 increases the A/T ratio by 46% compared to a reduction of -33% for the placebo.

Green: anagen Red: telogen

Before treatment

After treatment

a anagen hair e telogen hair

Day 0 4 months Day 0

Placebo HRS-10

4 months80

84

88

92

96

100

104 +13%

-2 %

# of

hai

rs/c

m2

Day 0 4 months Day 0

Placebo HRS-10

4 months0

10

20

30

40

50

60

70

-29 %

+23 %

# of

hai

rs/c

m2

A VER A GE A N A GEN HA IR DENSITY

A VER A GE TELOGEN HA IR DENSITY

Day 0 4 months Day 0

Placebo HRS-10

4 months0

1

2

3

4+46% *

-33%

Ana

gen/

Telo

gen

ratio

A N A GEN / TELOGEN R ATIO

* p<0.05

HRS-10