H6H

6-hydroxyhyoscyamine

H6H

ScopolamineHyoscyamine

Hyoscyamine 6-hydroxylase (H6H)

is bi-functional enzyme

C

H

CH2OH

PhR= .CO

H

OR

N-CH3

H

OR

N-CH3O

H

OR

N-CH3OH

H

Hyoscyamine 6-hydroxylase(H6H)

6-hydroxyhyoscyamine

-anticholinergic agent-first stimulates and then depresses the central nervous system-antispasmodic actions on smooth muscle

-anticholinergic agent-depresses the cerebral cortex-powerful hypnotic-slows the heart

Atropa belladonnaHyoscyamus albusScopolia japonicaMany Datura sp.

Hyoscyamus nigerDuboisia leichhardtiiDatura sanguinea

H6H

Scopolamine

Hyoscyamine

H

OR

N-CH3

H

OR

N-CH3OH

H

H

OR

N-CH3O

Metabolic Engineering of Hairy Rootsfor Tropane Alkaloid Production

Time Courses of H6H Expression in Hairy Roots