graphical abstract znonp tigriopus...graphical abstract znonp tigriopus.pptx author: mac created...
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Male
Egg sacs
Ovigerous fem
ale
0
20
40
60
80
100
CTR 15 30 60
Mean N° n
auplii/female
Copper µg/L
21 28
* * * *
*
0
20
40
60
80
100
120
CTR 15 30 60
Nau
plii an
d cope
podid %
Copper µg/L
Nauplii Copepodid
Nauplii and
copepodid aBer 6d exposure
* *
0
200
400
600
800
2 4 6 8 10 12 14
Cumula8
ve sum
of n
auplii
(inds/fem
ale)
Days
CTR 15 µg/L 30 µg/L 60 µg/L
* * * * * *
* * *
*
**
*
0
1
2
3
4
5
6
CTR 15 30 60
Broo
d nu
mbe
r/Female
Copper µg/L
* * *
0
5
10
15
20
CTR 15 30 60
Aborted egg sacs %
Copper µg/L
*
0
20
40
15 30 60 Inhibi8o
n
Copper µg/L
*
InhibiJon rate of copepodid
producJon aBer 6d compared to
control
0
5
10
15
20
CTR 15 30 60
Ovigerous fe
males % on 14d
Copper µg/L
* * *
0
5
10
15
20
CTR 15 30 60
N° n
auplii/broo
d
Copper µg/L
Tigriopus fulvus
Sublethal endpoints of copepoda
Sub-‐chronic test
0
20
40
60
80
100
120
15 30 60
Cope
podid mou
lt nu
mbe
r redu
c8on
(%)
Copper (µg/L)
**
0
5
10
15
20
25
30
0 0.05 0.1 0.15 0.2 0.25
Mea
n na
uplii
/bro
od
ZnO NP mg/L
!a!
c!!c!
!d!
!a!b!
0
40
80
120
160
200
0 0.05 0.1 0.15 0.2 0.25
Mea
n To
tal N
aupl
ii/Fe
mal
e
ZnO NP mg/L
b!
d!
c!
!a!
d!!e!
0
20
40
60
80
100
0 0.05 0.1 0.15 0.2 0.25
% N
on-v
iabl
e eg
gs/F
emal
e
ZnO NP mg/L
!a!a!!a!
!b!
!c!
b!
0!
1!
2!
3!
4!
0! 0.05! 0.1! 0.15! 0.2! 0.25!
Haching()m
e((days)(
ZnO(NP(mg/L(
!a,b!!a,b! !a,b!!a!
!b!!b!
9!
10!
11!
12!
13!
14!
0! 0.05! 0.1! 0.15! 0.2! 0.25!
(Ovigerous(fe
male(ap
pearan
ce()me(
(days)((m
ean±
sd)(
ZnO(NP(mg/L(
!a! a! !a!!a!
!c!
b!
0!
1!
2!
3!
4!
5!
6!
7!
8!
0! 0.05! 0.1! 0.15! 0.2! 0.25!
Mean(Broo
d/(fe
male(
ZnO(NP(mg/L(
!!e!
!a!b!
!b!!c!
!d!
Chronic effects of ZnO NP on mortality and reproduc8ve traits of the marine copepod Tigriopus
fulvus