GlnR

Schreier et al. 1991 J. Mol. Biol. 220:241Gutowski and Schreier 1992 J. Bacteriol 174:671Brown and Sonenshein 1996 J. Bacteriol 178:2450Zalieckas et al. 2006 J. Bacteriol 188:2578

GlnR controls transcription of glnRA operon in B. subtilis via two binding sites. TnrA does not act upon the first site.

lactococcus/streptococcus

lactobacillus/pediococcus/enterococcus

macrococcus/staphylococcus

Alignment of the upstream region of the glnRA operon in various species of the class Bacilli

bacillusgeo-paeni-lysini-oceano-anoxy-

leuconostoc/oenococcus

listeria

-35 -10GlnR

Bacilli (18)

Listeria (3)

Staphylococci (7)

Lactobacilli (8)

Leuconostoc (3)

Streptococci (12)

GlnR binding motifs derived from the alignment of the upstream region of the glnRA operon in various species of the class Bacilli

Eight sequence motif

>B1ATGTAAGAAAAACTAACAT>B2ATGTAAGAAAAACTAACAT>B3ATGTAAGAAAAGCTAACAT>B4ATGTTAGAAAAGCTAACAT>B5ATGTTAGGAATCCTTACAT>B6ATGTTAAGATTCCTTACAT>B7ATGTGAAGATTTGTGACAT>B8TTGTGATTTTTTGTGACAT

>LIS1ATGTTAGATTATCTTACAT>LIS2ATGTTAGATTATCTTACAT>LIS3ATGTTAGATTATCTTACAT>LIS4ATGTTAGATTATCTTACAT>LIS5ATGTTAGATTATCTTACAT>LIS6ATGTTAGAAAATCTTACAT>LIS7ATGTTAGAAAATCTTACAT>LIS8ATGTTAGAAAATCTTACAT

>Staph1ATGTAAGATTTCCTTACAT>Staph2ATGTAAGATTTCCTTACAT>Staph3ATGTTAGAAAACCTTACAT>Staph4ATGTTAGAAAACCTTACAT>Staph5ATGTTAGAAAACCTAACAT>Staph6ATGTTAGAAAACCTAACAT>Staph7ATGTCAGGAAACCTGACAT>Staph8ATGTCAGGAAACCTGACAT

>LAB1ATGTTAGAAAATCTAACAT>LAB2ATGTCAGAAAACCTAACAT>LAB3ATGTCAGAAAAGCTTACAT>LAB4GTGTGATATTTTCTAACAT>LAB5ATGTAAGAAAACCTTACAT>LAB6ATGTCAGAAAACCTAACAT>LAB7GTGTAAGAAAATATTACAT>LAB8TTGTGAGTTTTTCTAACAT

>LEU1ATGTTAGAAAACCTAACAC>LEU2ATGTTAGAAAACCTGACAC>LEU3ATGTTAGAAAACCTGACAC>LEU4ATGTTAGAAAACCTAACAC>LEU5ATGTTAGAAAACCTAACAC>LEU6ATGTTAGCTTTCCTAACAT>LEU7ATGTTAGCTTTCCTAACAT>LEU8ATGTTAGCTTTCCTAACAT

>STR1ATGTCAGAAAATCTAACAT>STR2ATGTCATAAAATCTAACAT>STR3ATGTCAGAAAATATAACAT>STR4ATGTAAGAAAATATAACAT>STR5ATGTAAGAAAATATCACAT>STR6ATGTTATATAATATCACAT>STR7ATGTTACATTTCGTGACAT>STR8GTGTTACTTTTAGTGACAT

Summary page of eight sequence motif

bacilli (- streptococci) streptococci

>1ATGTTAGAAAATCTAACAT>2ATGTTAGAAAATCTAACAT>3ATGTCAGAAAATCTTACAT>4ATGTCAGAAAACCTTACAT>5ATGTAAGAAAACCTCACAT>6ATGTAAGATTACATCACAT>7ATGTGATATTTGATGACAT>8GTGTGACGTTTAGTGACAT

Listeria and Leuconostoc left out because of small sample size

TnrA

>amtBATGTCAGGAAATCTTACAT>ansZ/yccCCTGAGAGAAAAGCTGACTG>gabPCTGGTATATTTTCTTACAC>glnQTGGGTATATTTTCTGACAA>nasAGTGTCACAAAAACTTACAC>nasBGTGTAAGTTTTTGTGACAC>nasDTTGTTACATTTTATAACAT>oppAATGGAAGAAAAACTAACGA>pucJTTGTTACATTTTCTTACAA>pucRATGTCAGTTTATGTAACAC>ykzBACGGAAGAAAAATTAACAA>ywrDTCGTCAGTTTTTCTGCCGG

Activating sites TnrA in B. subtilis

amtB

pucR