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GLICOSILACION DE PROTEINAS
Figure 13‐32 Molecular Biology of the Cell (© Garland Science 2008)
Figure 12‐1 Molecular Biology of the Cell (© Garland Science 2008)
Figure 12‐52 Molecular Biology of the Cell (© Garland Science 2008)
UDP-Glc
ALGUNAS SECUENCIAS CONSENSO PARA MODIFICACION POST-TRADUCCIONAL DE PROTEINAS.
1) N-Glicosilación: N*XT/S (X no puede ser Pro).
2) O-glicosilación: PXS*/T*XXP
3) Fosforilación: Ser/Thr: RRXS* ; KRXXS* ; K/RK/RXS*/T* ;
K/RS*/T*XK/R; K/RS*/T*PTyr: K/RXXXD/EXXXY*
4) Sulfatación: XE/DY*X
5) Prenilación: C*AAX ; C*C ; C*XC* (en el C-terminal).
6) Miristoilación: M-G*XXXS/T
Figure 12‐51 Molecular Biology of the Cell (© Garland Science 2008)
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n m l g f
d
1
1
113131
abc
e
i h
1
13 16
1314 14
jk
GlcNAc
Man
Glc
GI GII GII
GT
MI
MII
Tra
Transferencia y procesamiento del glicano
Control de calidad plegamiento de glicoproteinas
Estructura Calnexina
Golgi
Folded speciesGI GII
CNX /CRT
G3M9 G2M9 G1M9 M9
Rapid folding
ER Cytosol
ERADMRHOS-9
Irreversibly misfolded species
GIIα
ERmanI
CNX /CRT
CNX /CRT
M8
M7G1M7
G1M8
Slower folding
Slowest folding
ERmanIIGII is an in vivo regulator of misfolded/slow-folding glycoprotein
permanence in the ER
Figure 12‐1 Molecular Biology of the Cell (© Garland Science 2008)
Procesamiento N‐glicanos en el aparato de Golgi
Figure 13‐31 Molecular Biology of the Cell (© Garland Science 2008)
GlcNAc transferasas de N‐glicanos en Golgi de mamiferos
Formacion Enzima lisosomal‐Man 6‐P
Figure 13‐44 Molecular Biology of the Cell (© Garland Science 2008)
Figure 13‐32 Molecular Biology of the Cell (© Garland Science 2008)
Figure 12‐1 Molecular Biology of the Cell (© Garland Science 2008)
Caminos sintesis O‐glicanos en celulas de mamiferos
BIOSINTESIS DE LOS DETERMINANTES DE LOS GRUPOS SANGUINEOS A Y B
Figure 12‐1 Molecular Biology of the Cell (© Garland Science 2008)
Figure 12‐56 Molecular Biology of the Cell (© Garland Science 2008)
Figure 10‐14b Molecular Biology of the Cell (© Garland Science 2008)
Figure 19‐58 Molecular Biology of the Cell (© Garland Science 2008)
Control de calidad plegamiento de glicoproteinas
Figure 13‐44 Molecular Biology of the Cell (© Garland Science 2008)
Figure 19‐58 Molecular Biology of the Cell (© Garland Science 2008)
Figure 12‐1 Molecular Biology of the Cell (© Garland Science 2008)
