genway biotech nice expression system for lactococcus lactis · 2012-04-19 · mobitec is a partner...

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GenWay Biotech Your local Distributor: www.mobitec.com Phone: +49 (0)551-70722-0 Fax: +49 (0)551-70722-22 E-mail: [email protected] MoBiTec GmbH, Lotzestrasse 22a, 37083 Goettingen, Germany www.mobitec.com Phone: +49 (0)551-70722-0 Fax: +49 (0)551-70722-22 E-mail: [email protected] www.mobitec.com Phone: +49 (0)551-70722-0 Fax: +49 (0)551-70722-22 E-mail: [email protected] NICE ® Expression System for Lactococcus lactis Echelon , s assay services allow researchers to get quick and accurate results in the following areas: n Autotaxin (ATX) n PI3-Kinase (PI3-K) n Lysophosphatidic Acid (LPA) n Sphingosine 1 Phosphate (S1P) n Hyaluronic Acid (HA) n Collagen IV (CIV) Order Information Order No. Description Amount T-1300a-1-EC PI3-Ka Inhibitor Screening Services 20 ea T-1400-1-EC PI(3,4,5)P3 Phosphate (SHIP2) Assay Service 10 ea T-2300-1-EC PTEN Assay Service 10 ea T-1600-1-EC PTEN Fluorescence Polarization Assay Service 10 ea T-1700-1-EC Collagen IV Assay Service 20 ea T-1800-1-EC Sphingomyelinase Assay Service 10 ea T-1900-1-EC S1P Assay Service 20 ea T-2800-2-EC Lysophosphatidic Acid Assay Service 20 ea Echelon products and services are available in selected European countries only New strain for our effective & easy-to-operate expression system available Features n The system is fully food grade n No endotoxins are produced n No inclusion bodies n No spores n No extracellular proteinases n Tightly controlled gene expression allows production of toxic proteins n Simple fermentation, scale-up and downstream processing Areas of application n Production of homologous and heterologous pro- teins for food, feed, pharma and biocatalysis appli- cations n Production of prokaryotic and eukaryotic mem- brane proteins n Production of exo-polysaccharides n Production of ingredients through metabolic engi- neering: e.g. alanine, folate, diacetyl n Preparation of L. lactis as a biocatalyst by express- ion of a suitable enzyme as e.g. dehydrogenases and in situ co-factor regeneration n High throughput screening for enzyme evolution or enzyme comparison New Lactococcus lactis strain NZ9100 nisRnisK is integrated between pepQ and yqiD into a neutral locus. This means that all other aspects of this strain are fully functional including the pepN gene that has been used for the insertion of nisRnisK in NZ9000. Order Information Order No. Description Amount VS-ELS09100 NICE ® Lactococcus lactis expression Strain NZ9100 1 ml VS-ELV00500-01 NICE ® pNZ9530 Lactococcus lactis nisRnisK vector, in Strain NZ9000 1 ml VS-ELS09000-01 NICE ® Lactococcus lactis expression Strain NZ9000 1 ml VS-ELS03900-01 NICE ® Lactococcus lactis expression Strain NZ3900, food grade 1 ml VS-ELS10610-01 NICE ® E. coli host Strain MC1061 1 ml VS-ELV00100-01 NICE ® pNZ8008 Reference plasmid with gusA gene 10 µg VS-ELV00200-01 NICE ® pNZ8148 Lactococcus lactis expression vector, NcoI site 10 µg VS-ELV00250-01 NICE ® pNZ8150 Lactococcus lactis expression vector, ScaI site 10 µg VS-ELV00300-01 NICE ® pNZ8149 Lactococcus lactis expression vector, food grade, NcoI site 10 µg VS-ELK01000-02 NICE ® Nisin kit, 1 g nisin (concentration 2.5% (balance sodium chloride and denatured milk solids)), 1 ml 5% acetic acid 1 kit Lipid Research Assay Services from Echelon Order Information Order No. Description Amount RPP1075 REAL Mini System with SAF 75 RPP2075 REAL Mini System with ECOSAF 75 RPP3075 REAL Mini System with MIF 75 RPP4075 REAL Mini System with BAILENGER 75 RPP5075 REAL Mini System with VERONAL BUFFER 75 RPP6075 REAL Mini System empty 75 RP1713 REAL Graham Tests slides 90 Mini-System for Collection, Transport and Filtration of Parasites Convenient and safe handling of parasites Features n One system for safe collection, transportation and filtration of parasites n Available with different media or empty n Ready-to-use Graham‘s Test also available Description The filtration system is located inside the tube where the sample is collected, allowing to collect and filtrate the sam- ple, and recover the sediment in only one closed step. Tablespoon and glass beads are suitable for an easy col- lection and homogenization of the sample, and the double filter increases the power of filtration of the homogenized sample, resulting in cleaner sediment, with fewer impuri- ties for the analysis. The parasites are efficiently collected in the sedimentation cone by centrifugation and easily aspirated/pipetted for la- ter analysis by microscopy. MINI-SYSTEM FOR COLLECTING, TRANSPORT AND FILTRATION OF PARASITES NEW! Why not have your samples run by those who know the assays best? Features n 37,627 polyclonal and monoclonal antibodies n 16,922 proteins like antigens, native and recombinant proteins n 2,024 secondary antibodies n 2,444 peptides like blocking peptides and immunizing peptides n 640 ELISAs n Staining reagents, substrates, Western blots A provider of protein, antibody, and ELISA solutions Order Information Order No. Description Amount Antibodies 18-461-10667 Thyrotropin-releasing hormone receptor 0.05 ml 20-272-191956 Myosin cardiac heavy chain [3-48] 0.1 mg Proteins 10-663-45116 Acrp30 Mouse, Trimeric 0.01 mg 11-783-79480 Apolipoprotein A1 0.5 mg Secondary antibodies 25-732-294040 Affinity Purified Anti-Chicken IgG (H&L) (Rabbit) 10 mg 25-732-294122 Fluorescein Conjugated Affinity Purified Anti-Human IgG F(c) (Goat) 2 mg Peptides 06-785-213150 SEK1/MKK4 (Phospho-Ser80) Antibody Blocking Peptide 0.1 mg 06-271-83120 BNP (1-32) Human 1 mg ELISA kits 40-101-325007 Cortisol ELISA (Sheep) 1 kit 40-009-360001 Mycobacterium tuberculosis ELISA IgG, IgM, IgA Assay (Serum/Plasma) 1 kit Description MoBiTec is a partner of GenWay that offers high quality re- search products including proteins, antibodies, ELISA kits, research reagents and more. As a protein and antibody solutions provider, GenWay has a multi-functional technology platform specialized in using genes, proteins, antibodies, and their applications in cells, tissues and animal models for functional genomics and proteomics. GenWay’s core competencies enable the company to carry out bioinformatic analyses of protein tar- gets, produce high-quality protein antigens and ligands, and develop domain-specific IgY antibodies for immuno- affinity capture, partitioning, detection and analyses.

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Page 1: GenWay Biotech NICE Expression System for Lactococcus lactis · 2012-04-19 · MoBiTec is a partner of GenWay that offers high quality re - search products including proteins, antibodies,

GenWay Biotech

Your local Distributor:

www.mobitec.comPhone: +49 (0)551-70722-0Fax: +49 (0)551-70722-22E-mail: [email protected]

MoBiTec GmbH, Lotzestrasse 22a, 37083 Goettingen, Germany www.mobitec.com Phone: +49 (0)551-70722-0 Fax: +49 (0)551-70722-22 E-mail: [email protected] www.mobitec.com Phone: +49 (0)551-70722-0 Fax: +49 (0)551-70722-22 E-mail: [email protected]

NICE® Expression System for Lactococcus lactis

Echelon,s assay services allow researchers to get quick and accurate results in the following areas:

n Autotaxin (ATX)n PI3-Kinase (PI3-K)n Lysophosphatidic Acid (LPA)n Sphingosine 1 Phosphate (S1P)n Hyaluronic Acid (HA)n Collagen IV (CIV)

Order Information Order No. Description Amount T-1300a-1-EC PI3-Ka Inhibitor Screening Services 20 ea T-1400-1-EC PI(3,4,5)P3 Phosphate (SHIP2) Assay Service 10 ea T-2300-1-EC PTEN Assay Service 10 ea T-1600-1-EC PTEN Fluorescence Polarization Assay Service 10 ea T-1700-1-EC Collagen IV Assay Service 20 ea T-1800-1-EC Sphingomyelinase Assay Service 10 ea T-1900-1-EC S1P Assay Service 20 ea T-2800-2-EC Lysophosphatidic Acid Assay Service 20 eaEchelon products and services are available in selected European countries only

New strain for our effective & easy-to-operate expression system availableFeaturesn The system is fully food graden No endotoxins are producedn No inclusion bodiesn No sporesn No extracellular proteinasesn Tightly controlled gene expression allows production

of toxic proteinsn Simple fermentation, scale-up and downstream

processing

Areas of applicationn Production of homologous and heterologous pro-

teins for food, feed, pharma and biocatalysis appli- cations

n Production of prokaryotic and eukaryotic mem-brane proteins

n Production of exo-polysaccharidesn Production of ingredients through metabolic engi-

neering: e.g. alanine, folate, diacetyln Preparation of L. lactis as a biocatalyst by express-

ion of a suitable enzyme as e.g. dehydrogenases and in situ co-factor regeneration

n High throughput screening for enzyme evolution or enzyme comparison

New Lactococcus lactis strain NZ9100nisRnisK is integrated between pepQ and yqiD into a neutral locus. This means that all other aspects of this strain are fully functional including the pepN gene that has been used for the insertion of nisRnisK in NZ9000.

Order Information Order No. Description Amount VS-ELS09100 NICE® Lactococcus lactis expression Strain NZ9100 1 ml VS-ELV00500-01 NICE® pNZ9530 Lactococcus lactis nisRnisK vector, in Strain NZ9000 1 ml VS-ELS09000-01 NICE® Lactococcus lactis expression Strain NZ9000 1 ml VS-ELS03900-01 NICE® Lactococcus lactis expression Strain NZ3900, food grade 1 ml VS-ELS10610-01 NICE® E. coli host Strain MC1061 1 ml VS-ELV00100-01 NICE® pNZ8008 Reference plasmid with gusA gene 10 µg VS-ELV00200-01 NICE® pNZ8148 Lactococcus lactis expression vector, NcoI site 10 µg VS-ELV00250-01 NICE® pNZ8150 Lactococcus lactis expression vector, ScaI site 10 µg VS-ELV00300-01 NICE® pNZ8149 Lactococcus lactis expression vector, food grade, NcoI site 10 µg VS-ELK01000-02

NICE® Nisin kit, 1 g nisin (concentration 2.5% (balance sodium chloride and denatured milk solids)), 1 ml 5% acetic acid 1 kit

Lipid Research Assay Services from Echelon

Order Information Order No. Description Amount RPP1075 REAL Mini System with SAF 75 RPP2075 REAL Mini System with ECOSAF 75 RPP3075 REAL Mini System with MIF 75 RPP4075 REAL Mini System with BAILENGER 75 RPP5075 REAL Mini System with VERONAL BUFFER 75 RPP6075 REAL Mini System empty 75 RP1713 REAL Graham Tests slides 90

Mini-System for Collection, Transport and Filtration of ParasitesConvenient and safe handling of parasitesFeaturesn One system for safe collection, transportation

and filtration of parasitesn Available with different media or emptyn Ready-to-use Graham‘s Test also available

DescriptionThe filtration system is located inside the tube where the sample is collected, allowing to collect and filtrate the sam-ple, and recover the sediment in only one closed step.Tablespoon and glass beads are suitable for an easy col-lection and homogenization of the sample, and the double filter increases the power of filtration of the homogenized sample, resulting in cleaner sediment, with fewer impuri-ties for the analysis. The parasites are efficiently collected in the sedimentation cone by centrifugation and easily aspirated/pipetted for la-ter analysis by microscopy.

MINI-SYSTEM FOR COLLECTING,TRANSPORT AND FILTRATION

OF PARASITES

NEW!

Why not have your samples run by those who know the assays best?

Featuresn 37,627 polyclonal and monoclonal antibodiesn 16,922 proteins like antigens, native and recombinant

proteinsn 2,024 secondary antibodiesn 2,444 peptides like blocking peptides and immunizing

peptidesn 640 ELISAsn Staining reagents, substrates, Western blots

A provider of protein, antibody, and ELISA solutions

Order Information Order No. Description Amount Antibodies 18-461-10667 Thyrotropin-releasing hormone receptor 0.05 ml 20-272-191956 Myosin cardiac heavy chain [3-48] 0.1 mg Proteins 10-663-45116 Acrp30 Mouse, Trimeric 0.01 mg 11-783-79480 Apolipoprotein A1 0.5 mg Secondary antibodies 25-732-294040 Affinity Purified Anti-Chicken IgG (H&L) (Rabbit) 10 mg 25-732-294122 Fluorescein Conjugated Affinity Purified Anti-Human IgG F(c) (Goat) 2 mg Peptides 06-785-213150 SEK1/MKK4 (Phospho-Ser80) Antibody Blocking Peptide 0.1 mg 06-271-83120 BNP (1-32) Human 1 mg ELISA kits 40-101-325007 Cortisol ELISA (Sheep) 1 kit 40-009-360001 Mycobacterium tuberculosis ELISA IgG, IgM, IgA Assay (Serum/Plasma) 1 kit

DescriptionMoBiTec is a partner of GenWay that offers high quality re-search products including proteins, antibodies, ELISA kits, research reagents and more.As a protein and antibody solutions provider, GenWay has a multi-functional technology platform specialized in using genes, proteins, antibodies, and their applications in cells, tissues and animal models for functional genomics and proteomics. GenWay’s core competencies enable the company to carry out bioinformatic analyses of protein tar-gets, produce high-quality protein antigens and ligands, and develop domain-specific IgY antibodies for immuno-affinity capture, partitioning, detection and analyses.