Genomics:

READING genome sequences

ASSEMBLY of the sequence

ANNOTATION of the sequence

carry out dideoxy sequencing

connect seqs. to make whole chromosomes

find the genes!

“FIRST” generation sequencing

M.L. Metzker 2010. Nature Genetics Reviews 11, 31. First, 5 slides, in order of Figs.

Ion torrentIon torrent

NanoporeNanopore

5 Selected Technologies. 2nd, 3rd, and 4th generation sequencing.

Using Metzker, M.L. 2010, and T.P. Niedringhaus 2011Anal. Chem. 83, 4327.

• Solexa - - ->Illumina

Allows both directions: “pair-end”

Life: Roche/454; Polonator

• Soon to be bought by Thermo Fischer

NONE USED IN ROCHE/LIFE 454 PYROSEQUENCING

Ion Torrent (BIU)

• http://www.iontorrent.com/1-trillion-front-end/

Pacific Biosciences

New Generation:TemplateWith hairpinsOn each end

Nanopore DNA sequencing

http://lifetech-it.hosted.jivesoftware.com/videos/1016

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