GENETICAL FEATURES OF GENETICAL FEATURES OF CHROMOSOMAL FORMCHROMOSOMAL FORM ««CC»»

OF MICROTUS OF MICROTUS MAXIMOWICZIIMAXIMOWICZII FROM THE FROM THE

RUSSIAN FAR EAST RUSSIAN FAR EAST Irina V. Kartavtseva, Irina N. Sheremetyeva

Institute of Biology & Soil Science, Far Eastern Branch of Russian Academy of Sciences, Vladivostok (Russia)

Anastasia B. Ryabkova Khabarovsk Antiplague Station, (Russia)

Svetlana A. Romanenko, Olga L. Gladkikh Institute of Molecular and Cellular Biology SB RAS (Russia)

Lubov V. Frisman

Institute of Complex Analysis of Regional Problems Far Eastern Branch of Russian Academy of Sciences, Birobidzhan (Russia)

The role of chromosomal rearrangements in speciation still unclearly.

The maximowicz's vole Microtus maximowiczii (Rodentia) is convenient species for investigating this problem.

Introduction

Rearrangements by Mejer et al., 1996

1 - is fusion of two middle sized pairs of metacentrics to formating of large metacentric.

All rearrangements for this species can be divided into three types:

2 - is Robertsonian rearrangements.

3 - is pericentric inversions of 4 pairs.

Areal and terra typica points of Areal and terra typica points of M. maximowiczii M. maximowiczii

M. m. maximowiczii Schrenck 1858,

Amurskii and Khabarovskii regions in Russia, and in northeastern China

M. m. ungurensis Kastchenko 1913,

Buryatia and Chitinskii regions in Russia and North-Eastern Mongolia

ChinaMongolia

Russia

Korea

For this species was described five chromosomal forms (Kartavtseva et al., 2008):A : 2n = 39 - 44, NF = 53 - 60

(Buryatiya), B: 2n = 38 - 42, NF = 54 - 57 (Buryatiya), C: 2n = 39 - 41, NF = 55 - 60 (Far East) D: 2n= 40=43, NF= 54-58 (Zabaikalskii

kray and E. Mongolia)V: 2n = 36 - 40, NF = 52 - 56

(Transbaikalia and W.Mongolia).

Russia

Mongolia

China

«B»2n=38-42

«V»2n=40-43

«A»2n=39-44

«D»2n=40-43 «C»

2n=39-41

5 chromosomal forms of M. maximowiczii (Kartavtseva et al., 2008)

M. m. maximowiczii M. m. ungurensis

M. m. ungurensis

M. m. maximowiczii

"A“2n=39-44

"C“2n=39-41

control region mtDNA

Mmax45 Mmax41

Mmax38 Mmax39

Mmax42 Mmax40

Mmax37 Mmax43

Mmax44Mmax47

Mmax33 Mmax48 Mmax51

Mmax52Mmax49Mmax50

Mmax33 Mmax34

Mmax36 Mmax35

Mmax8Mmax11

Mmax7Mmax9 Mmax10

Mmax30 Mmax29

Mmax25 Mmax26

Mmax27Mmax31 Mmax28

Mmax32 Mmax1

Mmax2Mmax4

Mmax5 Mmax3

Mmax6 Mmax16 Mmax17

Mmax18 Mmax24

Mmax14 Mmax22 Mmax13 Mmax12 Mmax15 Mmax23 Mmax21 Mmax19 Mmax20

Microtus oeconomus

98

98

13

98

96

74

31

47

50

69

73

95

93

92

83

68

51

87

83

76

55

76

79

34

52

74

78

78

45

42

68

45

65

80

55

50

50

25

59

29

62

65

0.01

А

С

D

B

V

“Во

сто

чн

ая”

“Юж

ная

”“З

апад

ная

”

M. m. ungurensisM. m. ungurensis M. m. maximowiczii M. m. maximowiczii

Material Map of chromosomal investigation

We investigated karyotype of 36 individuals of Maximowicz's vole from six earlier not investigated populations of the territory Middle Amur river (chromosomal form C).

Literature data: 2n=42 2n=39

Our data: (2n=40-41) New data

2n=40-41

2n=40

2n=41

Chromosomal forms “D” and “C”

2n=402n=42

New chromosomal nomenclature of M. maximowiczii by fluorescent in situ hybridization (FISH)

M. maximowiczii FISH by Lemskaja et al., 2010 from chromosomal form “A”

M. maximowiczii (#2350)from Romanovka (chromosomal form “A”, our data)11

11,20

20

2n=41

M. Maximowiczii (#2060)from Norskii reservation (our data)

20

2011

11

Map of investigation of control region A B

mt DNA 940 np

A

A

B

Conclusion

In the territory Middle Amur river (chromosomal form C):

Stabilization of karyotype with Robertsonian rearrangement chromosomal pairs 11 and 20 and inversion of pair 14

Two clasters of control region of mt DNA can be results of isolations of two animals groups.

Thanks for attention