genetic variants of influenza ah1n1pdm09 virus in southern...
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Genetic variants ofinfluenzaAH1N1pdm09virusinSouthernMexico
PhD.GuadalupeAyoraTalaveraUniversidadAutónomadeYucatán
MISMSNov7-10,2016
Nuevo Leon
Yucatan
Federal District
Figure 1. Location of the Mexican states selected for the analyses. Nuevo Leon, the Fedral District and Yucatan are shaded in gray.
EpidemiologyofinfluenzainYucatan,Mexico
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J F M A M J J A S O N D
No.ofC
ases
EpidemiologicalWeek
pdm09Nationawide TotalInfYucatan pdm09Yucatan
GeneticvariantsoftheAH1N1pdm09virusin2013
HANAA/Yuc/66/2013 MayA/Yuc/79/2013 JuneA/Yuc/80/2013 JuneA/Yuc/81/2013 JuneA/Yuc/82/2013 JuneA/Yuc/116/2013 JulyA/Yuc/124/2013 JulyA/Yuc/128/2013 JulyA/Yuc/133/2013 JulyA/Yuc/134/2013 JulyA/Yuc/135/2013 JulyA/Yuc/137/2013 JulyA/Yuc/138/2013 JulyA/Yuc/141/2013 JulyA/Yuc/150/2013 JulyA/Yuc/152/2013 JulyA/Yuc/157/2013 JulyA/Yuc/214/2013 AugustA/Yuc/243/2013 AugustA/Yuc/250/2013 AugustA/Yuc/260/2013 AugustA/Yuc/286/2013 SeptemberA/Yuc/293/2013 SetemberA/Yuc/317/2013 SeptemberA/Yuc/329/2013 SeptemberA/Yuc/374/2013 October
A141TS162INone
A/Yuc/66/2013 MayA/Yuc/79/2013 JuneA/Yuc/80/2013 JuneA/Yuc/81/2013 JuneA/Yuc/82/2013 JuneA/Yuc/83/2013 JuneA/Yuc/86/2013 JuneA/Yuc/97/2013 JuneA/Yuc/101/2013 JuneA/Yuc/102/2013 JuneA/Yuc/104/2013 JuneA/Yuc/113/2013 JulyA/Yuc/116/2013 JulyA/Yuc/123/2013 JulyA/Yuc/124/2013 JulyA/Yuc/128/2013 JulyA/Yuc/133/2013 JulyA/Yuc/134/2013 JulyA/Yuc/135/2013 JulyA/Yuc/137/2013 JulyA/Yuc/138/2013 JulyA/Yuc/141/2013 JulyA/Yuc/150/2013 JulyA/Yuc/152/2013 JulyA/Yuc/157/2013 JulyA/Yuc/214/2013 AugustA/Yuc/243/2013 AugustA/Yuc/250/2013 AugustA/Yuc/260/2013 AugustA/Yuc/286/2013 SeptemberA/Yuc/293/2013 SeptemberA/Yuc/317/2013 SeptemberA/Yuc/329/2013 SeptemberA/Yuc/346/2013 SeptemberA/Yuc/374/2013 October
N341SL206S+N341SI321VL206SI34V+K432ENone
• HA Fig. 2
• NAFig. 4
Fig. 1
Antigenic Site CaConsideredinducecross-
reactiveprotection
Antigenic Site SaConsidered inducestrain-
specific protection
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ases
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A(H1N1)pdm09 A(H3N2) Infuenza B
- TheA(H1N1)pdm09viruswasunderstrongselectivepressuredueeitherto(1)theco-circulationofinfluenzaH3N2viruses,phenomenonthatwasnotobservedsincetheemergencyofthenovelvirusin2009,and/orto(2)thehostimmunepressure.
- ThesubstitutionA141Tisnearthereceptorbindingsite,iftheaminoacidchangeaffectedthereceptoraffinityofthevirus,thenitattainedacompensatorymutationinNA–N341S,inordertosustainvirustransmission.
- However,theshortperiodofcirculationoftheseviralvariantssuggeststhattheycouldhavebeenlesstransmissibleandunabletopersistoverthedominantstrain.Stilltheviruswasunderstrongselectivepressure.
A141T
S162I• Toovercomethis,asecondchangeinadifferentsitewasacquired,accompaniedinsomevirusesbyanovelmutationintheNA- L206S,withunknownproperties.
• Basedonouranalysisandthelowfrequenciesatwhichthesemutationshaveoccurredworldwide(<1%),suggestnoneofthesemutationswerefixedonthepopulationandtheselectivepressureofthevaccinationcampaignforthe2013-2014seasonmighthaveproducedviralextinctionunderabottleneckevent.
• Since2014,viruseshavemutatedatS162N– glycosilation site.
Submitted to PLosOne
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