general stem cell biology brochure

General Stem Cell Biology

Infinite Expansion....Infinite Potential

n Stem Cell Resources

n Stem Cell Pathways

n Stem Cell Markers

n Stem Bio Overview

n Protocols

http://www.sigmaaldrich.com?cm_mmc=social-_-scribd-_-gen%20stem%20cell%20bio-_-pdf

http://www.sigma-aldrich.com/life-science.html?cm_mmc=social-_-scribd-_-gen%20stem%20cell%20bio-_-pdf

2 ORDER: 800-325-3010 TECHNICAL SERVICE: 800-325-5832sigma-aldrich.com

Gen

eral

Ste

m C

ell B

iolo

gy

Stem cells hold the key to a number of cellular processes from development, tissue regeneration, and aging. They also hold the promise of cures for many diseases and injuries as well as offer an opportunity to bridge the ‘animal to human gap’ in ADMET and drug efficacy studies, a necessary step in provid-ing therapeutic products for human use. The potential of stem cells is certain, however bringing this potential to fruitition is contingent upon several key factors, not the least of them being the highly motivated and inteligent global research

community. These scientists have and continue to contribute key incremental discoveries on nearly a daily basis and these discoveries move the entire science closer and closer to becom-ing the numerous miracle therapies that we have always been predicted. The stem cell biology platform, as presented in this brochure, highlights the most common general use media, supplements, reagents, and tools for stem cell discovery culture and analysis. Additional products can be found throughout the Sigma-Aldrich® catalog and website.

Stem Cell BiologyInfinite Expansion....Infinite Potential

Isolation Characterization Expansion DifferentiationFunctionalProfiling

In vivoTracking

Inner Cell Mass

Bone Marrow

Blood

Bone

Muscle

Lung

Liver

Intestine

Pancreas

Stomach

Esophagus

Skin

Neural Tissue

Germ Cells

Mesoderm

Endoderm

Ectoderm

ESC’s

Stem Cell Fate


Our Innovation, Your Research — Shaping the Future of Life Science 3

General Stem

Cell Biology

In lineage tree 1, the development of stem cells occurs after the formation of germ layers. These stem cells are thus restricted by germ layer commitment to their respective lineage (e.g., mesoderm is formed, giving rise to hematopoietic progenitors that become hematopoietic stem cells). Lineage tree 2 illustrates

the area that stem cells might develop similarly to PGCs, in that they avoid the lineage commitments during gastrulation and subsequently migrate to specific tissue and organ niches.

(adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)

Origins and Differentiation of Tissues

Nervous tissue/NSCs

Skin/skin SCs

Bone marrow and blood/HSCs and MSCs

Muscle and bone/tissue SC

Lung, liver, and pancreas/organ speci�c SCs

Esophagus, stomach, and intestine/intestinal SCs

Ectoderm

Primordial germ cells

Mesoderm

Endoderm1

Nervous tissue

Skin

Bone marrow and blood

Muscle and bone

Lung, liver, and pancreas

Esophagus, stomach, and intestine

NSCs/skin SCs

HSCs/MSCs/tissue SCs

Organ SCs/intestinal SCs

Ectoderm

Primordial germ cells

Mesoderm

Endoderm

Multipotent stem cells

2

Gastrulation

Gastrulation

Stem Cell BiologyInfinite Expansion....Infinite Potential

Putative developmental ontogeny of stem cells



Gen

eral

Ste

m C

ell B

iolo

gy

Genomic reprogramming in the oocyte, early embryos, and the germ line


Blastocyst (E7.5)

Maternal inheritance& regulation of pluripotency

Initiation of imprinting

Migrating PGCs

Epigenetic reprogramming

Genital ridgeE11.5

ES cells EG cells

Speci�cationof germ cells

Founder PGC (E7.5)

STELLAOCT-4ESG-1NANOGSOX2

STELLAOCT-4ESG-1

EZH2EEDHP1

Oocyte

DNA demethylationX re-activation

erasure of imprints

Presenting!

Prestige Antibodies™

Antibodies that work, right out of the box!

The most highly characterized antibodies in the industry – 1,800 NEW Prestige Antibodies, powered by Atlas Antibodies

n Designed & validated by the Human Proteome Resource (HPR)

n Standardized in universal protocols

n Over 500 immunohistochemical images for every antibody

n Publicly available data on the web

Look even closer. Go to sigma.com/prestige for more information.

Our innovation, Your Research – Shaping the Future of Life SciencePrestige Antibodies is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.


http://www.sigma-aldrich.com/prestige?cm_mmc=social-_-scribd-_-gen%20stem%20cell%20bio-_-pdf



General Stem

Cell Biology

Workflow Product Class Product Number Product NameIsolation Cryopreservation C6164 Cell Freezing Medium-DMSO 1×

Isolation Cryopreservation C6295 Cell Freezing Medium-DMSO Serum free 1x

Isolation Cryopreservation C6039 Cell Freezing Medium-Glycerol 1×

Isolation Cryopreservation C2639 Cell Freezing Medium-Serum-free 1×

Isolation Cryopreservation D2650 Dimethyl sulfoxide

Isolation Dissociation E8008 EDTA solution

Isolation Dissociation E8140 Elastase from human leukocytes

Isolation Enzymes C2014 Collagenase, rat islet isolation

Isolation Enzymes C6079 Collagenase + Protease Inhibitors, suitable for islet isolation

Isolation Enzymes C9572 Collagenase, g-irradiated

Isolation Enzymes C9722 Collagenase, sterile, cell culture

Isolation Enzymes D4818 Dispase I

Isolation Enzymes D4693 Dispase II

Isolation Isolation a0561 ACCUSPIN™ System-Histopaque®-1077 Box of 100 tubes. Each 50 ml tube contains 15 ml Histopaque 1077-1 and will separate between 15 to 30 ml of anticoagulated blood.

Isolation Isolation A6929 ACCUSPIN System-Histopaque-1077 Box of 40 tubes. Each 12 ml tube contains 3 ml of Histopaque 1077-1 and will separate between 3 to 6 ml of anticoagulated blood.

Isolation Isolation A7054 ACCUSPIN System-Histopaque-1077 Box of 12 tubes. Each 50 ml tube contains 15 ml of Histopaque 1077-1 and will separate between 15 to 30 ml of anticoagulated blood.

Isolation Isolation F1418 Ficoll® 400 SigmaUltra, Type 400-DL, lyophilized powder

Isolation Isolation F2878 Ficoll PM 70 Type 70

Isolation Isolation F4375 Ficoll PM 400 Type 400, lyophilized powder

Isolation Isolation F5415 Ficoll solution Type 400, 20% in H2O

Isolation Isolation F8016 Ficoll 400 Type 400-DL, lyophilized powder, cell culture tested

Isolation Isolation F8636 Ficoll 400 lyophilized powder, γ-irradiated, cell culture tested

Isolation Isolation F9378 Ficoll 400 Type 400-DL, lyophilized powder

Isolation Isolation H8889 Histopaque-1077 Hybri-Max™ liquid, sterile-filtered, hybridoma tested

Isolation Reagents C6164 Cell Freezing Medium-DMSO

Characterization Antibodies A2726 Anti-ADAM-10, Cytoplasmic Domain antibody produced in rabbit

Characterization Antibodies A2851 Anti-ADAM-10, Prohormone Convertase Activation Site antibody produced in rabbit

Characterization Antibodies A2976 Anti-ADAM-12, N-terminal antibody produced in rabbit

Characterization Antibodies A3101 Anti-ADAM-10, Propeptide Domain antibody produced in rabbit

Characterization Antibodies A3226 Anti-ADAM-10, N-Terminal antibody produced in rabbit

Characterization Antibodies A3976 Anti-ADAM-17, Activation Site antibody produced in rabbit

Characterization Antibodies A4226 Anti-ADAM-17, Propeptide Domain antibody produced in rabbit

Characterization Antibodies A4351 Anti-ADAM-17, Cytoplasmic Domain antibody produced in rabbit

Characterization Antibodies A5102 Anti-Aurora B antibody produced in rabbit

Product ListIn some instances scientific milestones appear as serendipitous, nearly accidental discoveries. But for most of us discovery is a process and series of small steps that are the result of a long and wide pathway of techniques, methods, and in-depth analysis. In order to serve you better we have listed our the relevant products in the following pages by ‘work flow’ which is essentially addressing all of the steps in your discovery process. For every area of study, including regenerative medicine, there is a step-wise and systematic ap-proach in gathering data. The proceeding products have been listed to appear in order of process: Isolation – products for enzymatic or gradient cell separation as well as storage/cryopreservation Characterization – product for cell identification Expansion – product to increase cell populations Differentiation – products to move cells forward along a given lineage and confirm lineage markers Functional profiling – products to assist in converting or diverting the intercellular processes, mechanisms, and pathways In vitro/in vivo analysis – products for tagging/marking cells in vitro and in vivo


In vivoTracking



Gen

eral

Ste

m C

ell B

iolo

gy

Workflow Product Class Product Number Product NameCharacterization Antibodies A5228 Anti-Smooth muscle actin, produced in mouse

Characterization Antibodies A5691 Anti-Smooth muscle alpha-actin - alkaline phosphatase, produced in mouse

Characterization Antibodies B3428 Anti-Bax antibody produced in rabbit

Characterization Antibodies B8429 Monoclonal Anti-Bax antibody produced in mouse, 2 mg/mL

Characterization Antibodies B9553 Monoclonal Anti-Bone Morphogenetic Protein 2

Characterization Antibodies C1366 Anti-CXCR3 antibody produced in rabbit

Characterization Antibodies C2542 Monoclonal Anti-N-Cadherin antibody produced in mouse





Characterization Antibodies C3960 Anti-Ciliary Neurotrophic Factor antibody, human, produced in goat

Characterization Antibodies C4085 Anti-Ciliary Neurotrophic Factor antibody, rat, produced in goat

Characterization Antibodies C4585 Monoclonal Anti-β-Tubulin−Cy3 antibody produced in mouse

Characterization Antibodies C5588 Anti-Cyclin D1 antibody produced in rabbit

Characterization Antibodies C6198 Anti-Smooth muscle alpha-actin - Cy3

Characterization Antibodies C7930 Anti-CD3, T Cell antibody produced in rabbit whole antiserum

Characterization Antibodies C8227 Anti-CXCR4 (Fusin), N-Terminal antibody produced in rabbit

Characterization Antibodies C8352 Anti-CXCR4 (Fusin) (182-196) antibody produced in rabbit

Characterization Antibodies C8354 Anti-CX3CR-1, N-Terminal antibody produced in rabbit

Characterization Antibodies C8479 Anti-CX3CR-1 (175-189) antibody produced in rabbit

Characterization Antibodies C9080 Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse

Characterization Antibodies D2442 Anti-Dopamine Transporter antibody produced in rabbit

Characterization Antibodies D4567 Anti-DNMT1, human, rat, mouse, antibody produced in rabbit

Characterization Antibodies D4692 Anti-DNMT1, human, mouse, antibody produced in rabbit

Characterization Antibodies D5567 Anti-dimethyl-Histone H3 (diMe-Lys9), hu, mo, rat, bovine, chicken, frog, Drosophila, C. elegans, A. thaliana produced in rabbit

Characterization Antibodies D5692 Anti-dimethyl-Histone H3 (diMe-Lys4) hu, mo, rat, bovine, chicken, frog, Drosophila, C. elegans, A. thaliana, produced in rabbit

Characterization Antibodies D9442 Anti-Dopamine Transporter, Extracellular Loop 2 antibody produced in rabbit

Characterization Antibodies D9567 Anti-Dopamine Transporter, C-Terminus antibody produced in rabbit

Characterization Antibodies E160 Anti-Eotaxin antibody produced in goat

Characterization Antibodies E1906 Anti-EAPII (TTRAP) antibody produced in rabbit

Characterization Antibodies F0300 Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit

Characterization Antibodies F0425 Anti-Fibroblast Growth Factor Receptor-3, Cytoplasmic antibody produced in rabbit

Characterization Antibodies F0550 Anti-Flt-3 antibody produced in goat

Characterization Antibodies F2043 Monoclonal Anti-β-Tubulin−FITC antibody produced in mouse

Characterization Antibodies F3304 Anti-Frizzled-2 antibody produced in rabbit

Characterization Antibodies F3393 Anti-Fibroblast Growth Factor-Basic (1-24) antibody produced in rabbit

Characterization Antibodies F3520 Anti-vonWillebrand Factor produced in rabbit

Characterization Antibodies F3648 Anti-Fibronectin antibody produced in rabbit

Characterization Antibodies F3777 Anti-Smooth muscle alpha-actin - FITC, produced in mouse

Characterization Antibodies F3804 Anti-Frizzled-1 antibody produced in rabbit

Characterization Antibodies F3922 Anti-Fibroblast Growth Factor Receptor-3, Extracellular antibody produced in rabbit

Characterization Antibodies F5421 Anti-Fibroblast Growth Factor Receptor-1 antibody produced in rabbit

Characterization Antibodies F5521 Anti-Fibroblast Growth Factor-Acidic antibody produced in rabbit

Product List, cont.


In vivoTracking



General Stem

Cell Biology

Workflow Product Class Product Number Product NameCharacterization Antibodies F5537 Anti-Fibroblast Growth Factor-Basic antibody produced in rabbit

Characterization Antibodies F5900 Monoclonal Anti-Interleukin-2−FITC antibody produced in mouse, clone 5334.2

Characterization Antibodies F6796 Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit

Characterization Antibodies F8925 Anti-Flt-3/Flk-2 ligand (FL) antibody, rec mo

Characterization Antibodies F9049 Anti-Fibroblast Growth Factor-10 antibody produced in goat

Characterization Antibodies F9050 Anti-Flt-3/Flk-2 ligand (FL) antibody produced in goat

Characterization Antibodies F9304 Anti-FBI-1/Pokemon antibody produced in rabbit

Characterization Antibodies F9429 Anti-FBI-1/Pokemon (C-terminal) antibody produced in rabbit

Characterization Antibodies G0157 Anti-GABA Transporter GAT-1 antibody produced in rabbit

Characterization Antibodies G0290 Anti-GATA1 antibody produced in rabbit

Characterization Antibodies G0907 Anti-Granulocyte-Macrophage Colony Stimulating Factor

Characterization Antibodies G1544 Anti-GFP, N-terminal antibody produced in rabbit

Characterization Antibodies G4154 Anti-Granulocyte-Macrophage Colony Stimulating Factor Receptor α

Characterization Antibodies G4165 Anti-gp130 antibody produced in rabbit

Characterization Antibodies G4420 Anti-GRP94 (C-terminal) antibody produced in rabbit

Characterization Antibodies G4545 Anti-GRP94 antibody produced in rabbit


Characterization Antibodies G8043 Anti-phospho-Growth Associated Protein-43 (pSer41) antibody produced in rabbit

Characterization Antibodies G8044 Anti-GATA-4 (pSer105) antibody produced in rabbit


Characterization Antibodies G8918 Anti-GRP78/BiP (GL-19) antibody produced in rabbit

Characterization Antibodies G9043 Anti-GRP78/BiP (ET-21) antibody produced in rabbit

Characterization Antibodies G9152 Anti-Galactocerebroside antibody produced in rabbit whole antiserum

Characterization Antibodies G9269 Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit

Characterization Antibodies H0163 Monoclonal Anti-Histone Deacetylase 4 (HDAC4) antibody produced in mouse

Characterization Antibodies H0164 Anti-Histone H3 antibody produced in rabbit

Characterization Antibodies H0412 Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit

Characterization Antibodies H0788 Monoclonal Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in mouse

Characterization Antibodies H0913 Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in mouse

Characterization Antibodies H2039 Anti-HP1β antibody produced in rabbit

Characterization Antibodies H2164 Anti-HP1α antibody produced in rabbit

Characterization Antibodies H2287 Anti-Histone Deacetylase 6 (HDAC6) antibody produced in rabbit

Characterization Antibodies H2537 Anti-Histone Deacetylase 7 (HDAC7) (YS-19) antibody produced in rabbit

Characterization Antibodies H2662 Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit






Characterization Antibodies H5912 Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit


Characterization Antibodies H6409 Monoclonal Anti-phospho-Histone H3 (pSer10) antibody produced in mouse




Product List, cont.


In vivoTracking



Gen

eral

Ste

m C

ell B

iolo

gy

Workflow Product Class Product Number Product NameCharacterization Antibodies H7162 Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit

Characterization Antibodies H7664 Anti-phospho-Histone H1.4 (pSer27) antibody produced in rabbit

Characterization Antibodies H7789 Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit


Characterization Antibodies H8163 Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit

Characterization Antibodies H9286 Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit

Characterization Antibodies H9411 Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in rabbit

Characterization Antibodies H9536 Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in rabbit

Characterization Antibodies H9663 Anti-Histone Deacetylase 5 (HDAC5) (PI-16) antibody produced in rabbit

Characterization Antibodies H9908 Monoclonal Anti-phospho-Histone H3 (pSer28) antibody produced in rat

Characterization Antibodies I0505 Anti-IκBα antibody produced in rabbit

Characterization Antibodies I0649 Monoclonal Anti-Interleukin-6 Receptor antibody produced in mouse

Characterization Antibodies I1142 Monoclonal Anti-Interferon-γ antibody produced in rat

Characterization Antibodies I1404 Anti-Interleukin-1 Receptor Accessory Protein, C-Terminal antibody produced in rabbit

Characterization Antibodies I1644 Anti-Interleukin-5 antibody produced in goat

Characterization Antibodies I2143 Anti-Interleukin-6 antibody produced in rabbit

Characterization Antibodies I2407 Anti-Interleukin-3 Receptor α antibody produced in goat

Characterization Antibodies I2519 Monoclonal Anti-Interleukin-8 antibody produced in mouse

Characterization Antibodies I2532 Monoclonal Anti-Interleukin-7 antibody produced in mouse

Characterization Antibodies I2657 Anti-Interleukin-5 Receptor α antibody produced in goat


Characterization Antibodies I2907 Monoclonal Anti-Interleukin-7 Receptor α antibody produced in mouse

Characterization Antibodies I2908 Monoclonal Anti-Interferon-γ antibody produced in hamster

Characterization Antibodies I3392 Anti-Interleukin-1α antibody produced in goat

Characterization Antibodies I3393 Anti-Interleukin-6 antibody produced in rabbit

Characterization Antibodies I3642 Monoclonal Anti-Interleukin-1β antibody produced in mouse

Characterization Antibodies I3653 Anti-Interferon β antibody produced in goat

Characterization Antibodies I3767 Anti-Interleukin-1β antibody produced in goat

Characterization Antibodies I3778 Monoclonal Anti-Interleukin-1α antibody produced in mouse

Characterization Antibodies I4153 Anti-Interleukin-12 Receptor β1 antibody produced in goat

Characterization Antibodies I4902 Anti-Interferon-α/β Receptor 1 antibody produced in goat

Characterization Antibodies I5018 Anti-Interleukin-1α antibody produced in rabbit

Characterization Antibodies I5027 Anti-Interferon-γ (IFN-γ) antibody produced in goat

Characterization Antibodies I5152 Anti-Interferon-γ Receptor I (IFN-γ RI) produced in goat



Characterization Antibodies I5527 Monoclonal Anti-Interleukin-1 Soluble Receptor Type I antibody produced in mouse

Characterization Antibodies I5652 Monoclonal Anti-Interleukin-2 Soluble Receptor α antibody produced in mouse

Characterization Antibodies I5777 Monoclonal Anti-Interleukin-2 Soluble Receptor β antibody produced in mouse

Characterization Antibodies I5902 Monoclonal Anti-Interleukin-2 Soluble Receptor γ antibody produced in mouse

Characterization Antibodies I6152 Anti-Interleukin-2 Soluble Receptor α antibody produced in goat

Characterization Antibodies I6277 Anti-Interleukin-2 Soluble Receptor β antibody produced in goat

Characterization Antibodies I6402 Anti-Interleukin-2 Soluble Receptor γ antibody produced in goat



Product List, cont.


In vivoTracking



General Stem

Cell Biology

Workflow Product Class Product Number Product NameCharacterization Antibodies I7901 Monoclonal Anti-Interleukin-6 antibody produced in mouse, clone 6708.11


Characterization Antibodies I8153 Anti-Interleukin-1 Receptor Accessory Protein antibody produced in rabbit



Characterization Antibodies I8638 Monoclonal Anti-Integrin β1 antibody produced in mouse

Characterization Antibodies I9016 Anti-Interferon-γ antibody produced in goat

Characterization Antibodies I9141 Anti-Interferon-γ (IFN-γ) antibody produced in goat


Characterization Antibodies IMMH2 Anti-Smooth Muscle Actin Immunohistology Kit

Characterization Antibodies J3877 Anti-JAK3 antibody produced in rabbit

Characterization Antibodies J4127 Anti-Jagged 1 antibody produced in goat

Characterization Antibodies K4763 Anti-Ku70 antibody produced in rabbit

Characterization Antibodies K4888 Anti-Ku80 (XRCC5) (557-570) produced in rabbit

Characterization Antibodies L4418 Anti-LSD1 (AOF2) (C-terminal) antibody produced in rabbit

Characterization Antibodies L4793 Anti-LSD1 (AOF2) (N-terminal) antibody produced in rabbit

Characterization Antibodies L9152 Anti-Leukemia Inhibitory Factor antibody produced in goat

Characterization Antibodies L9277 Anti-Leukemia Inhibitory Factor antibody produced in goat

Characterization Antibodies M2420 Monoclonal Anti-Monocyte Chemotactic Protein-1 produced in mouse

Characterization Antibodies M254 Anti-Monocyte Chemotactic Protein 2 antibody produced in goat

Characterization Antibodies M255 Anti-Monocyte Chemotactic Protein 3 produced in goat

Characterization Antibodies M257 Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu

Characterization Antibodies M257 Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu.

Characterization Antibodies M4819 Anti-methyl-Histone H3 (Me-Lys4) antibody produced in rabbit

Characterization Antibodies M5917 Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. mo.

Characterization Antibodies M6042 Anti-Macrophage Inflammatory Protein-1α antibody produced in goat, rec. hu.

Characterization Antibodies M6792 Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu.

Characterization Antibodies M6917 Anti-Macrophage Inflammatory Protein-1α antibody produced in goat, rec.mo.

Characterization Antibodies M7273 Anti-Microtubule-Associated Proteins (MAPs) antibody produced in rabbit

Characterization Antibodies M7786 Anti-Smooth muscle myosin, heavy chain, produced in mouse

Characterization Antibodies N0649 Anti-Neuronal Specific Enolase antibody produced in rabbit

Characterization Antibodies N3038 Monoclonal Anti-Nanog produced in mouse

Characterization Antibodies N3279 Monoclonal Anti-Nerve Growth Factor-β antibody produced in mouse

Characterization Antibodies N3908 Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in rabbit

Characterization Antibodies N4142 Anti-Neurofilament 200 antibody produced in rabbit

Characterization Antibodies N6655 Anti-Nerve Growth Factor 2.5S antibody produced in rabbit

Characterization Antibodies N6786 Monoclonal Anti-Notch1 antibody produced in mouse

Characterization Antibodies N8523 Monoclonal Anti-NF-κB antibody produced in mouse

Characterization Antibodies N8773 Anti-Nerve Growth Factor-β antibody produced in goat

Characterization Antibodies N9773 Anti-Neurotrophin-3 antibody produced in goat

Characterization Antibodies P0873 Anti-Oct4 produced in rabbit

Characterization Antibodies P2110 Anti-Platelet-Derived Growth Factor Receptor α antibody produced in goat

Characterization Antibodies P2229 Anti-Platelet-Derived Growth Factor Soluble Receptor β antibody produced in goat

Characterization Antibodies P5498 Anti-Pro-NGF antibody produced in rabbit

Characterization Antibodies P5976 Anti-Platelet-Derived Growth Factor-BB antibody produced in goat

Product List, cont.


In vivoTracking



Gen

eral

Ste

m C

ell B

iolo

gy

Workflow Product Class Product Number Product NameCharacterization Antibodies P6101 Anti-Platelet-Derived Growth Factor antibody produced in goat

Characterization Antibodies P6847 Monoclonal Anti-Interleukin-2−R-Phycoerythrin antibody produced in mouse

Characterization Antibodies R125 Anti-RANTES antibody produced in goat

Characterization Antibodies R2903 Anti-RALT/Mig-6 (SG-16) antibody

Characterization Antibodies R3028 Anti-RALT/MIG-6 (PE-16) antibody

Characterization Antibodies R5027 Anti-RIP140 antibody produced in rabbit

Characterization Antibodies R9528 Anti-RUNX1 antibody

Characterization Antibodies S192 Anti-SDF-1/PBSF antibody produced in goat

Characterization Antibodies S193 Anti-Synapsin I antibody produced in rabbit

Characterization Antibodies S2562 Anti-Stem Cell Factor Receptor (SCF R) antibody

Characterization Antibodies S2644 Anti-S-100 antibody produced in rabbit

Characterization Antibodies S2687 Anti-SDF-1β/PBSF antibody, recombinant human SDF-1β/PBSF expressed in E. coli, produced in goat, affinity isolated antibody

Characterization Antibodies S2822 Anti-Synapsin II antibody produced in rabbit

Characterization Antibodies S2947 Anti-Synapsin II (C-terminal) antibody produced in rabbit

Characterization Antibodies S4072 Anti-Sirt3 antibody produced in rabbit


Characterization Antibodies S5313 Anti-Sir2 (AS-16) antibody produced in rabbit


Characterization Antibodies S7693 Anti-Sox2 antibody produced in rabbit

Characterization Antibodies S7696 Anti-Sec23 antibody produced in rabbit

Characterization Antibodies S8316 Monoclonal Anti-SUV39H1 Histone Methyltransferase antibody produced in mouse ~2 mg/mL, clone 44.1, purified immunoglobulin, buffered aqueous solution

Characterization Antibodies T0198 Monoclonal Anti-β-Tubulin antibody produced in mouse, ~1 mg/mL, clone D66, purified immunoglobulin, buffered aqueous solution

Characterization Antibodies T0563 Anti-Transforming Growth Factor-α antibody, produced in goat

Characterization Antibodies T0688 Anti-Transforming Growth Factor-β1 antibody produced in chicken

Characterization Antibodies T0951 Anti-Tet Repressor (TETR) antibody produced in rabbit

Characterization Antibodies T1940 Anti-Transforming Growth Factor-β Soluble Receptor III antibody produced in goat

Characterization Antibodies T1941 Anti-TrkB antibody produced in goat

Characterization Antibodies T2200 Anti-β-Tubulin III antibody produced in rabbit

Characterization Antibodies T2565 Anti-Transforming Growth Factor-β Soluble Receptor II antibody produced in goat

Characterization Antibodies T2817 Anti-Tie 1 antibody produced in goat affinity isolated antibody

Characterization Antibodies T3192 Anti-Tie 2 antibody produced in goat

Characterization Antibodies T4026 Monoclonal Anti-β-Tubulin antibody produced in mouse, clone TUB 2.1, ascites fluid

Characterization Antibodies T4442 Anti-Transforming Growth Factor-β2 antibody produced in goat



Characterization Antibodies T4930 Anti-Thrombopoietin antibody produced in goat

Characterization Antibodies T5076 Monoclonal Anti-β-Tubulin Isotype III antibody produced in mouse, ~2 mg/mL, clone SDL.3D10, purified immunoglobulin, buffered aqueous solution

Characterization Antibodies T5201 Monoclonal Anti-β-Tubulin antibody produced in mouse, ~2 mg/mL, clone Tub2.1, purified immunoglobulin, buffered aqueous solution

Characterization Antibodies T5293 Monoclonal Anti-β-Tubulin antibody produced in mouse, clone 2-28-33, ascites fluid

Characterization Antibodies T5442 Anti-ADAM-17 (TACE), C-Terminal antibody produced in rabbit

Characterization Antibodies T7576 Anti-TGN46 antibody produced in rabbit

Characterization Antibodies T8660 Monoclonal Anti-β-Tubulin III antibody produced in mouse, clone SDL.3D10, ascites fluid

Characterization Antibodies T9429 Anti-Transforming Growth Factor-β, pan antibody produced in rabbit

Product List, cont.


In vivoTracking



General Stem

Cell Biology

Workflow Product Class Product Number Product NameCharacterization Antibodies T9826 Anti-TGN38 antibody produced in rabbit

Characterization Antibodies U3254 Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat

Characterization Antibodies V1014 anti-Vascular Endothelial Growth Factor Receptor-2 from goat

Characterization Antibodies V1014 Anti-Flk1, Vascular Endothelial Growth Factor Receptor 2 antibody

Characterization Antibodies V1514 Anti-VE-Cadherin antibody produced in rabbit

Characterization Antibodies V2258 Monoclonal Anti-Vimentin antibody produced in mouse, clone LN-6, ascites fluid

Characterization Antibodies V4630 Anti-Vimentin antibody produced in goat, whole antiserum

Characterization Antibodies V5255 Monoclonal Anti-Vimentin antibody produced in mouse , clone VIM-13.2, ascites fluid

Characterization Antibodies V5764 Anti-Vesicular GABA Transporter antibody produced in rabbit

Characterization Antibodies V6389 Monoclonal Anti-Vimentin antibody produced in mouse, ~1 mg/mL, clone V9, purified immunoglobulin, buffered aqueous solution

Characterization Antibodies V6630 Monoclonal Anti-Vimentin antibody produced in mouse, clone V9, ascites fluid

Characterization Antibodies V7881 Monoclonal Anti-Vitronectin antibody produced in mouse

Characterization Antibodies W2141 Anti-Wnt-1 antibody produced in goat

Characterization Antibodies W2266 Anti-Wnt-4 antibody produced in goat

Characterization Antibodies W2516 Monoclonal Anti-Wnt-3a antibody produced in mouse

Characterization/Differentiation Marker/Receptors F8674 Fas/Fc Chimera human, recombinant, expressed in mouse NSO cells

Characterization/Differentiation Marker/Receptors F8799 Fas/Fc Chimera from mouse, recombinant, expressed in Sf21 cells

Characterization/Differentiation Proteins/Peptides A1229 Angiopoietin-3 from mouse recombinant, expressed in mouse NSO cells

Characterization/Differentiation Proteins/Peptides A1479 Angiopoietin-4 human recombinant, expressed in mouse NSO cells,

Characterization/Differentiation Proteins/Peptides A3156 Bovine Serum Albumin, globulin-free, g-irradiated

Characterization/Differentiation Proteins/Peptides A3782 Human Serum Albumin, fatty acid-free, 99%

Characterization/Differentiation Proteins/Peptides A4941 Activin A, recombinant, human

Characterization/Differentiation Proteins/Peptides A5226 Angiopoietin-1 human, recombinant, expressed in mouse NSO cells

Characterization/Differentiation Proteins/Peptides A5843 Human Serum Albumin, low endotoxin

Characterization/Differentiation Proteins/Peptides A6955 Angiogenin human, recombinant, expressed in Escherichia coli

Characterization/Differentiation Proteins/Peptides A7223 Human Serum Albumin, recombinant, from pichia pastoris

Characterization/Differentiation Proteins/Peptides A7979 Bovine Serum Albumin, 35% solution in DPBS

Characterization/Differentiation Proteins/Peptides A8806 Bovine Serum Albumin, fatty acid-free

Characterization/Differentiation Proteins/Peptides A9847 Angiopoietin-2 human, recombinant, expressed in mouse NSO cells

Characterization/Differentiation Proteins/Peptides A9975 ADAM-10 from mouse, recombinant, expressed in Sf21 cells

Characterization/Differentiation Proteins/Peptides B1182 Bcl-2 human recombinant, expressed in Escherichia coli

Characterization/Differentiation Proteins/Peptides B1436 BAX Inhibiting Peptide V5

Characterization/Differentiation Proteins/Peptides E2153 E-Cadherin/Fc Chimera from mouse

Characterization/Differentiation Proteins/Peptides H0786 Heregulin-β, EGF Domain human, recombinant, expressed in Escherichia coli

Characterization/Differentiation Proteins/Peptides H5529 Heregulin-α, EGF Domain human, recombinant, expressed in Escherichia coli

Characterization/Differentiation Proteins/Peptides H7660 Heregulin-β1 (EGF Domain) human, recombinant, expressed in Escherichia coli

Characterization/Differentiation Proteins/Peptides I3265 Interferon-γ human

Characterization/Differentiation Proteins/Peptides I3275 Interferon-γ from rat

Characterization/Differentiation Proteins/Peptides I4151 Interferon β 1a human

Characterization/Differentiation Proteins/Peptides I4276 Interferon-αA human

Characterization/Differentiation Proteins/Peptides I4401 Interferon-αA/D human

Characterization/Differentiation Proteins/Peptides I4777 Interferon-γ from mouse

Characterization/Differentiation Proteins/Peptides I4784 Interferon from human leukocytes

Characterization/Differentiation Proteins/Peptides I8654 Interferon-γ porcine

Characterization/Differentiation Proteins/Peptides I8657 Interferon α from rat

Product List, cont.


In vivoTracking



Gen

eral

Ste

m C

ell B

iolo

gy

Workflow Product Class Product Number Product NameCharacterization/Differentiation Proteins/Peptides I8782 Interferon αA from mouse

Characterization/Differentiation Proteins/Peptides I8907 Interferon β from rat

Characterization/Differentiation Proteins/Peptides I9032 Interferon β from mouse

Characterization/Differentiation Proteins/Peptides n6784 Noggin, mouse recombinant

Characterization/Differentiation Proteins/Peptides N8911 Notch-3/Fc Chimera from mouse

Characterization/Differentiation Proteins/Peptides N9036 Notch-3/Fc Chimera human

Characterization/Differentiation Proteins/Peptides N9161 Notch-2/Fc Chimera from rat

Characterization/Differentiation Proteins/Peptides N9268 Notch-1/Fc Chimera from rat

Characterization/Differentiation Proteins/Peptides O8137 Osteoprotegerin/Fc Chimera from mouse

Characterization/Differentiation Proteins/Peptides O9631 Osteoprotegerin/Fc Chimera human

Expansion/Differentiation Adhesion/Attachment/Matrices C0543 Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Expansion/Differentiation Adhesion/Attachment/Matrices C1809 Collagen from kangaroo tail

Expansion/Differentiation Adhesion/Attachment/Matrices C3867 Collagen, Type I solution from rat tail Approx. 100mg/vial

Expansion/Differentiation Adhesion/Attachment/Matrices C5533 Collagen from human placenta

Expansion/Differentiation Adhesion/Attachment/Matrices C7661 Collagen I from rat tail

Expansion/Differentiation Adhesion/Attachment/Matrices C8919 Collagen from calf skin solution, sterile-filtered, 1 mg/mL

Expansion/Differentiation Adhesion/Attachment/Matrices C9301 Collagen from chicken sternal cartilage powder

Expansion/Differentiation Adhesion/Attachment/Matrices C9791 Collagen from calf skin powder

Expansion/Differentiation Adhesion/Attachment/Matrices E1270 ECM gel from Engelbreth-Holm-Swarm murine sarcoma

Expansion/Differentiation Adhesion/Attachment/Matrices E2153 E-Cadherin/Fc Chimera from mouse

Expansion/Differentiation Adhesion/Attachment/Matrices E2278 E-Cadherin/Fc Chimera human

Expansion/Differentiation Adhesion/Attachment/Matrices F0635 Fibronectin from rat plasma powder

Expansion/Differentiation Adhesion/Attachment/Matrices F0895 Fibronectin from human plasma 0.1% (Solution),

Expansion/Differentiation Adhesion/Attachment/Matrices F1141 Fibronectin from bovine plasma solution, 1 mg/mL

Expansion/Differentiation Adhesion/Attachment/Matrices F2006 Fibronectin from human plasma

Expansion/Differentiation Adhesion/Attachment/Matrices F2518 Fibronectin from human foreskin fibroblasts Cellular

Expansion/Differentiation Adhesion/Attachment/Matrices F4759 Fibronectin from bovine plasma lyophilized powder

Expansion/Differentiation Adhesion/Attachment/Matrices H4777 Heparan sulfate proteoglycan

Expansion/Differentiation Adhesion/Attachment/Matrices L2020 Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Expansion/Differentiation Adhesion/Attachment/Matrices L6274 Laminin from human placenta

Expansion/Differentiation Adhesion/Attachment/Matrices O2260 Osteopontin from mouse, recombinant, expressed in mouse NSO cells

Expansion/Differentiation Adhesion/Attachment/Matrices O3514 Osteopontin from bovine milk

Expansion/Differentiation Adhesion/Attachment/Matrices O4264 Osteopontin human recombinant, expressed in mouse NSO cells

Expansion/Differentiation Adhesion/Attachment/Matrices P5864 Proteoglycan from bovine nasal septum

Expansion/Differentiation Adhesion/Attachment/Matrices P5989 Proteoglycan from chicken sternal cartilage

Expansion/Differentiation Adhesion/Attachment/Matrices S7439 L-Selectin/Fc Chimera from mouse

Expansion/Differentiation Adhesion/Attachment/Matrices V0132 Vitronectin, from rat plasma

Expansion/Differentiation Adhesion/Attachment/Matrices V8379 Vitronectin from human plasma

Expansion/Differentiation Adhesion/Attachment/Matrices V9881 Vitronectin from bovine plasma

Expansion/Differentiation Antibiotics P4333 streptomycin/penicillin G, 100 ×, stabilized

Expansion/Differentiation Antibiotics G1272 Gentamicin solution, 10 mg/mL

Expansion/Differentiation Antibiotics P0781 Penicillin-Streptomycin, 100 ×

Expansion/Differentiation Classical Media D5796 DMEM

Expansion/Differentiation Classical Media D8062 DMEM

Expansion/Differentiation Classical Media D8537 DPBS without Ca and Mg


In vivoTracking

Product List, cont.



General Stem

Cell Biology

Workflow Product Class Product Number Product NameExpansion/Differentiation Classical Media I3390 IMDM

Expansion/Differentiation Classical Media M0644 MEM

Expansion/Differentiation Classical Media M4530 Med 199



Expansion/Differentiation Classical Media R8758 RPMI

Expansion/Differentiation Cyokines/Growth Factors/Chemokines

B1434 Bone Morphogenetic Protein 7 human, recombinant, expressed in CHO cells


B2680 BMP-4


B3555 Bone Morphogenetic Protein 2 (BMP-2) human, recombinant, expressed in Escherichia coli,cell culture tested


B3795 Brain-derived neurotrophic factor human recombinant, expressed in Escherichia coli


C3710 Ciliary Neurotrophic Factor human, ≥97% (SDS-PAGE), recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested


C3835 Ciliary Neurotrophic Factor from rat, ≥98% (SDS-PAGE and HPLC), recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested


C8615 CXCL16 human recombinant, expressed in Escherichia coli


E1257 Epidermal Growth Factor from murine submaxillary gland, cell culture tested


E1388 β-Endothelial Cell Growth Factor human recombinant, expressed in Escherichia coli


E4127 Epidermal Growth Factor from murine submaxillary gland


E4269 LONG™ EGF human, recombinant, expressed in Escherichia coli


E4643 Heparin-Binding EGF-Like Growth Factor human recombinant, expressed in Sf21 cells


E8399 Eotaxin-3 human, recombinant, expressed in Escherichia coli,


e8403 Eotaxin−Biotin from mouse


E9152 Eotaxin-2 from mouse, recombinant, expressed in Escherichia coli


E9644 Epidermal Growth Factor human recombinant, expressed in Escherichia coli


F0291 Fibroblast Growth Factor-Basic human (1)


F1168 Fibroblast Growth Factor-9 human


F1802 Fibroblast Growth Factor-8c from mouse


F1927 Fibroblast Growth Factor Receptor 2β (IIIc)/Fc Chimera from mouse


F2052 Fibroblast Growth Factor Receptor 2β (IIIb)/Fc Chimera from mouse


f3133 Fibroblast Growth Factor from bovine pituitary


F3422 Flt-3/Flk-2 ligand human, recombinant, expressed in Escherichia coli






F5392 Fibroblast Growth Factor-Basic from bovine pituitary (1)


F5542 Fibroblast Growth Factor-Acidic human


In vivoTracking

Product List, cont.



Gen

eral

Ste

m C

ell B

iolo

gy

Workflow Product Class Product Number Product NameExpansion/Differentiation Cyokines/Growth Factors/

ChemokinesF5895 Fibroblast Growth Factor Basic Fragment 1-24 bovine (1)


F6926 Fibroblast Growth Factor-8b from mouse








F8675 Fibroblast Growth Factor Receptor-4/Fc Chimera human


F8924 Fibroblast Growth Factor 10 human, recombinant, expressed in Escherichia coli


F9174 Fibroblast Growth Factor Receptor-1α (IIIc)/Fc Chimera human


F9175 Flt-3/Flk-2 Ligand from mouse (1)


F9299 Fibroblast Growth Factor Receptor-1β (IIIc)/Fc Chimera human


F9424 Fibroblast Growth Factor Receptor-2α (IIIb)/Fc Chimera human


F9549 Fibroblast Growth Factor Receptor-2α (IIIc)/Fc Chimera human


F9647 Fibroblast Growth Factor Receptor 2β (IIIc)/Fc Chimera from mouse


F9786 Fibroblast Growth Factor-Basic Heparin Stabilized human (1)


F9799 Fibroblast Growth Factor Receptor-2β (IIIc)/Fc Chimera human


F9924 Fibroblast Growth Factor Receptor-3α (IIIc)/Fc Chimera from mouse


G0282 Granulocyte-Macrophage Colony-Stimulating Factor from mouse, recombinant, expressed in Escherichia coli, lyophilized powder, ≥97% (SDS-PAGE), cell culture tested (Sigma)


G0407 Granulocyte Colony-Stimulating Factor


G0792 Granulocyte-Macrophage Colony-Stimulating Factor from rat


G5035 Granulocyte-Macrophage Colony-Stimulating Factor from human


G7545 gp130 Soluble Fragment human


G8160 Granulocyte Colony-Stimulating Factor


H1404 HGF, recombinant, human


H9661 HGF, recombinant, human


I0406 Interleukin-6 from rat recombinant, expressed in Escherichia coli


I0523 Interleukin-2 from mouse, recombinant, expressed in Escherichia coli


I0779 Interleukin-2 Soluble Receptor α human, recombinant, expressed in mouse NSO cells


I0904 Interleukin-2 Soluble Receptor β human, recombinant, expressed in Sf21 cells


I1029 Interleukin-2 Soluble Receptor γ human, recombinant, expressed in Sf21 cells


I1145 Interleukin-5 from mouse, recombinant, expressed in Sf21 cells


I1271 LONG™ R3 IGF-1, Recombinant Analog human (1)

Product List, cont.


In vivoTracking



General Stem

Cell Biology


ChemokinesI1395 Interleukin-6 human recombinant, expressed in Escherichia coli


I1645 Interleukin-8 human, recombinant, expressed in Escherichia coli


I1646 Interleukin-3 human recombinant, expressed in Escherichia coli


I2276 Interleukin-12 human, recombinant, expressed in Sf21 cells


I2393 Interleukin-1β from rat, recombinant, expressed in Escherichia coli




I2526 Insulin-like Growth Factor-II human (1)




i2656 Insulin-like Growth Factor-I (E3R) human (1)


I2778 Interleukin-1α human, recombinant, expressed in Escherichia coli


I3263 Insulin-like Growth Factor-II fragment 33-40 (1)


I3268 Interleukin-6 human from human (osteosarcoma cells),


I3657 Insulin-Like Growth Factor Binding Protein-2 from mouse (1)


I3769 IGF-1, recombinant, human


I3901 Interleukin-1α from rat, recombinant, expressed in Escherichia coli


I4144 Interleukin-3 from mouse recombinant, expressed in Escherichia coli


I4638 Interleukin-1β Fragment 163-171 human, synthetic


I4892 Interleukin-7 from mouse, recombinant, expressed in Escherichia coli


I5271 Interleukin-1β from mouse, recombinant, expressed in Escherichia coli




I5277 Interferon-γ Receptor 1 human


I5396 Interleukin-1α from mouse, recombinant, expressed in Escherichia coli


I5403 Insulin-Like Growth Factor Binding Protein-2 human (1)


I5771 Interleukin-6 Receptor Soluble Fragment human recombinant, expressed in Sf21 cells




I7389 Interleukin-3 human recombinant, expressed in Escherichia coli


I7908 Interleukin-2 human


I8148 Interleukin-1 Soluble Receptor, Type II human


I8523 Interleukin-12 from mouse, recombinant, expressed in Sf21 cells


I8779 Insulin-like Growth Factor-I from mouse (1)


I8904 Insulin-like Growth Factor-II from mouse (1)

Product List, cont.


In vivoTracking



Gen

eral

Ste

m C

ell B

iolo

gy


ChemokinesI9279 Interleukin-11 from mouse, recombinant, expressed in Escherichia coli


I9401 Interleukin-1β human, recombinant, expressed in Escherichia coli


I9646 Interleukin-6 from mouse recombinant, expressed in Escherichia coli


I9780 Interleukin-2/Fc Chimera (non-lytic) human, recombinant, expressed in NS.1 cells


I9905 Interleukin-2/Fc Chimera (non-lytic) from mouse, recombinant, expressed in CHO cells


L3408 Transforming Growth Factor-β1 Latency Associated Peptide human, recombinant, expressed in Sf21 cells


L5158 LIF (Leukemia Inhibitory Factor), recombinant, mouse


L5283 LIF (Leukemia Inhibitory Factor), recombinant, human


M1439 MIG from mouse


M252 MIG human


M261 Platelet-Derived Growth Factor-BB from rat


M6167 Macrophage Inflammatory Protein-1α from mouse, recombinant, expressed in Escherichia coli


M6292 Macrophage Inflammatory Protein-1α human, recombinant, expressed in Escherichia coli


M6417 Macrophage Inflammatory Protein-1β human, recombinant, expressed in Sf21 cells


M6542 Macrophage Inflammatory Protein-1β from mouse, recombinant, expressed in Escherichia coli


N0513 Nerve Growth Factor-7S from murine submaxillary gland


N1408 Nerve Growth Factor-β human recombinant


N1780 Neurotrophin-4 human


N1905 Neurotrophin-3 human


N2513 Nerve Growth Factor-β from rat recombinant


N6009 Nerve Growth Factor-2.5S from murine submaxillary gland


N8133 Nerve Growth Factor from Vipera lebetina venom


N8898 Nerve Growth Factor Receptor/Fc Chimera human recombinant


N9411 Nerve growth factor receptor/Fc chimera from mouse recombinant


P1985 Platelet-Derived Growth Factor Soluble Receptor α human


P3076 Platelet-Derived Growth Factor-AA human


P3201 Platelet-Derived Growth Factor-BB human


P3326 Platelet-Derived Growth Factor-AB human


P3618 Platelet-Derived Growth Factor Receptor β/Fc Chimera human


P4056 Platelet-Derived Growth Factor-BB from rat

Product List, cont.


In vivoTracking



General Stem

Cell Biology


ChemokinesP4306 Platelet-Derived Growth Factor-BB human


P8147 Platelet-Derived Growth Factor from human platelets


P8953 Platelet-Derived Growth Factor from porcine


R2274 RANTES from mouse


R6267 RANTES human


S190 Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor human, recombinant, expressed in Escherichia coli,


S2437 SCF Soluble Receptor human


S5816 Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse recombinant, expressed in Escherichia coli


S7901 Stem Cell Factor human recombinant


S8406 Stromal Cell-Derived Factor 1β/pre-B Cell Growth Stimulating Factor human, recombinant, expressed in Escherichia coli,


S9915 Stem Cell Factor from mouse recombinant


T0892 Interleukin-2 from rat spleenocytes


T1566 Tie-1/Fc Chimera human, recombinant, expressed in mouse NSO cells


T1654 Transforming Growth Factor-β1 from human platelets


T2815 Transforming Growth Factor-β2 human, recombinant, expressed in NSO cells


T2926 Transforming Growth Factor-β5 amphibian, recombinant, expressed in Sf21 cells


T3448 Tie-2/Fc Chimera from mouse, recombinant, expressed in mouse NSO cells


T3698 Transforming Growth Factor-β Receptor II/Fc Chimera from mouse, recombinant, expressed in mouseNSO cells


T4567 Transforming Growth Factor-β Soluble Receptor III human, recombinant, expressed in mouse NSO cells


T5050 Transforming Growth Factor-β1 from porcine platelets


T5278 Transforming Growth Factor-α from rat, g-irradiated


T5300 Transforming Growth Factor-β2 from porcine platelets


T5425 Transforming Growth Factor-β3 human, recombinant, expressed in Sf21 cells


T7039 Transforming Growth Factor-β1 human, recombinant, expressed in insect cells


T7039 Transforming Growth Factor-β1 human, recombinant, expressed in insect cells


T7924 Transforming Growth Factor-α human, recombinant, expressed in Escherichia coli


T8569 TrkC/Fc Chimera human recombinant, expressed in mouse NSO cells


T8694 TrkB/Fc Chimera human recombinant, expressed in mouse NSO cells,


T9533 Transforming Growth Factor-α from rat


V1260 VEGF Receptor-3 (Flt-4)/Fc Chimera human

Product List, cont.


In vivoTracking



Gen

eral

Ste

m C

ell B

iolo

gy


ChemokinesV3388 Vascular Endothelial Growth Factor 121 human (1)


V3513 Vascular Endothelial Growth Factor 120 from mouse (1)


V3638 Vascular Endothelial Growth Factor 164 from rat (1)


V4512 Vascular Endothelial Growth Factor from mouse (1)


V6012 Vascular Endothelial Growth Factor D human (1)


V6633 VEGF Receptor-3 (Flt-4)/Fc Chimera from mouse


V6758 VEGF Receptor-2(Flk-1, KDR)/Fc ChimeraVEGF Receptor-2


V7259 Vascular Endothelial Growth Factor human (1)

Expansion/Differentiation Enzymes F2803 Flt-3/Fc Chimera, rec mo

Expansion/Differentiation Enzymes F7426 Flt-3/Fc Chimera human, recombinant, expressed in mouse NSO cells,

Expansion/Differentiation Enzymes L0164 LYN B human, recombinant, expressed in insect cells

Expansion/Differentiation Enzymes L9913 LYN A human, recombinant, expressed in insect cells

Expansion/Differentiation Specialty Media 51800C Claycomb medium

Expansion/Differentiation Stemline Media S0189 Stemline HSC

Expansion/Differentiation Stemline Media S0192 Stemline HSC

Expansion/Differentiation Stemline Media S0196 Stemline Keratinocyte

Expansion/Differentiation Stemline Media S1569 Stemline MSC

Expansion/Differentiation Stemline Media S1694 Stemline T cell

Expansion/Differentiation Stemline Media S3194 Stemline Neural Stem Cell Expansion medium

Expansion/Differentiation Stemline Media S3444 Stemline DC

Expansion/Differentiation Stemline Media S9945 Stemline Keratinocyte Medium Supplement with BPE

Expansion/Differentiation Vitamins, Hormones, Misc. A4034 sodium ascorbate (Takahashi, 2003)

Expansion/Differentiation Vitamins, Hormones, Misc. A6791 Atrial Natriuretic Peptide fragment 3-28 rat

Expansion/Differentiation Vitamins, Hormones, Misc. D4902 Dexamethasone, cell culture tested

Expansion/Differentiation Vitamins, Hormones, Misc. D8893 Dexamethasone, γ-irradiated, cell culture tested

Expansion/Differentiation Vitamins, Hormones, Misc. R0635 Retinol Acetate - soluble

Expansion/Differentiation Vitamins, Hormones, Misc. R2625 retinoic acid

Expansion/Differentiation Vitamins, Hormones, Misc. R7882 Retinol Acetate

Expansion/Differentiation Vitamins, Hormones, Misc. T1568 Thrombopoietin human, recombinant, expressed in Escherichia coli

Expansion/Differentiation Vitamins, Hormones, Misc. T4184 Thrombopoietin from mouse, recombinant, expressed in NSO cells

Functional Profiling Inhibitors P4484 Purvalanol A

Functional Profiling Ion Channels 17783 Calcein-AM

Functional Profiling Kit BLOT2 Northern Blots, poly(A)+ RNA from BALB/c mouse, multiple tissue

Functional Profiling Kit BLOT3 Northern Blots, poly(A)+ RNA from BALB/c murine embryo

Functional Profiling Kit BPQ24 BioPORTER® QuikEase™ Protein Delivery Kit

Functional Profiling Kit BPQ96 BioPORTER QuikEase Protein Delivery Kit

Functional Profiling Kit CSAA1 Panorama® Antibody Microarray - Cell Signaling Kit

Functional Profiling Kit D7440 JumpStart™ Taq ReadyMix™ for Quantitative PCR

Functional Profiling Kit DMN10 GenElute™ Direct mRNA Miniprep Kit sufficient for 10 purifications

Functional Profiling Kit DNAQF DNA Quantitation Kit, Fluorescence Assay

Functional Profiling Kit G1N10 GenElute Mammalian Genomic DNA Miniprep Kit sufficient for 10 purifications

Functional Profiling Kit GRAA2 Panorama Antibody Microarray - Gene Regulation I Kit

Product List, cont.


In vivoTracking



General Stem

Cell Biology

Workflow Product Class Product Number Product NameFunctional Profiling Kit HPFM4 Panorama® Human Protein Function Array -Signal Transduction

Functional Profiling Kit ID0010 In vitro Director™ PCR System 3′ FLAG®

Functional Profiling Kit ID0020 In vitro Director PCR System 5′ c-Myc

Functional Profiling Kit ID0030 In vitro Director PCR System 5′ FLAG

Functional Profiling Kit ID0040 In vitro Director PCR System 5′ Anchor™

Functional Profiling Kit MRN10 GenElute mRNA Miniprep Kit sufficient for 10 purifications

Functional Profiling Kit N5659 Northern Bound™ Human Alzheimer’s Total RNA Blot II from human

Functional Profiling Kit N5784 Northern Bound Human mRNA Blot

Functional Profiling Kit N5909 Northern Bound Human mRNA Blot II

Functional Profiling Kit N7409 Northern Bound Human Normal Brain Total RNA Blot

Functional Profiling Kit N7534 Northern Bound Human Normal Brain Total RNA Blot II

Functional Profiling Kit NA2000 GenElute Blood Genomic DNA Kit sufficient for 10 purifications

Functional Profiling Kit PPAA4 Panorama Antibody Array -p53 pathways

Functional Profiling Kit R2653 RapidTransit™ Transformation Kit

Functional Profiling Kit RTN10 GenElute Mammalian Total RNA Miniprep Kit sufficient for 10 purifications

Functional Profiling Kit SH0211 MISSION® shRNA Human G-Protein Coupled Receptors, Glycerol Set-1

Functional Profiling Kit SH0231 MISSION shRNA Human G-Protein Coupled Receptors, Lentiviral Transduction Particle Set 1

Functional Profiling Kit SH0511 MISSION shRNA Human Tumor Suppressors: Glycerol Set-1

Functional Profiling Kit SH0531 MISSION shRNA Human Tumor Suppressors: Lentiviral Transduction Particle Set

Functional Profiling Kit SH0831 MISSION shRNA Human Cytokines and Chemokines, Lentiviral Transduction Particle Set 1

Functional Profiling Kit SH1011 MISSION shRNA Human Ion Channels: Glycerol Set-1

Functional Profiling Kit SH1031 MISSION shRNA Human Ion Channels, Lentiviral Transduction Particle Set 1

Functional Profiling Kit SH1311 MISSION shRNA Human Cytokine and Chemokine Receptors, Glycerol Set-1

Functional Profiling Kit WGA1 GenomePlex® Whole Genome Amplification (WGA) Kit

Functional Profiling Kit WGA4 GenomePlex Single Cell Whole Genome Amplification Kit

Functional Profiling Kit WTA1 TransPlex® Whole Transcriptome Amplification Kit

Functional Profiling Kit XNAB2 Extract-N-Amp™ Blood PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications

Functional Profiling Kit XNABRE REDExtract-N-Amp™ Blood PCR Kit sufficient for 10 extractions, sufficient for 10 amplifications

Functional Profiling Reagent D5758 Diethyl pyrocarbonate ≥97% (NMR)

Functional Profiling Transfection/Transduction N2913 N-TER Nanoparticle siRNA Transfection System

Functional Profiling Transfection/Transduction NPT01 NeuroPorter™ Transfection Kit

Functional Profiling Transfection/Transduction SHC002 MISSION Non-Target shRNA Control Transduction Particles

Functional Profiling Transfection/Transduction SHC002V MISSION Non-Target shRNA Control Vector

Functional Profiling Transfection/Transduction SHC003 MISSION TurboGFP™ Control Vector

Functional Profiling Transfection/Transduction SHC003V MISSION TurboGFP Control Transduction Particles

In vitro & In vivo Tracking Stains/Syes 32857 Wright Stain solution

In vitro & In vivo Tracking Stains/Syes D8417 DAPI

In vitro & In vivo Tracking Stains/Syes G9641 Giemsa stain

In vitro & In vivo Tracking Stains/Syes HT90T Gram stain for tissue

In vitro & In vivo Tracking Stains/Syes M5655 MTT

In vitro & In vivo Tracking Stains/Syes P1951 Phalloidin–Tetramethylrhodamine B isothiocyanate Phalloidin from Amanita phalloides

In vitro & In vivo Tracking Stains/Syes P5282 Phalloidin, Fluorescein Isothiocyanate Labeled Phalloidin from Amanita phalloides

In vitro & In vivo Tracking Stains/Syes T8154 Trypan Blue solution 0.4%, liquid, sterile-filtered, cell culture tested

In vitro & In vivo Tracking Stains/Syes X4626 XTT

Product List, cont.


In vivoTracking



Gen

eral

Ste

m C

ell B

iolo

gy

Category Marker Function/Pathway Gene PN SA Antibody

ASCAdipose-derived mesenchymal stem cell

ASC, PSC CXCR4 chemokine cell surface receptor CXCR4 C8227 Anti-CXCR4 (Fusin), N-Terminal

ASC, PSC ATP Binding Cassette G2 transporter protein ABCG2 B7165 Anti-Breast Cancer Resistance Protein

ASC, PSC ATP Binding Cassette G2 transporter protein ABCG2 B7059 Monoclonal Anti-Breast Cancer Resistance Protein

ASC, PSC ATP Binding Cassette G2 transporter protein ABCG2 B7684 Monoclonal Anti-Breast Cancer Resistance Protein

AGPC Glial Fibrillary Acidic Protein Intermediate filament protein GFAP G9269 Anti-Glial Fibrillary Acidic Protein

AGPC Glial Fibrillary Acidic Protein Intermediate filament protein GFAP G3893 Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP)

BSCOsteoblast mesenchymal stem cell, periosteum progenitor

CM GATA-4 Cardiomyocyte differentiation through BMP GATA4 G8044Anti-phospho-GATA4 (pSer105) antibody produced in rabbit

CM Cardiac troponin I TNNI3

EB Embryoid body

EB, HSC, CM Flk1 Cell surface receptor KDR V1014 Anti-Vascular Endothelial Growth Factor Receptor 2

EB, HSC, CM Flk1 Cell surface receptor KDR V9134Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2

EB, HSC, CM Flk1 Cell surface receptor KDR V3003Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)

EGC Embryonic germ cell

Epithelial SC Cytokeratin 19 Epithelial intermediate filament protein KRT19 C6930 Monoclonal Anti-Cytokeratin Peptide 19

Epithelial SC p63 Trp63 P3362 Monoclonal Anti-p63

Epithelial SC p63 Trp63 P3737 Monoclonal Anti-p63

ERC Endometrial Regenerative Cell

ERC, BSC, MSC, ASC, SMPC Endoglin (CD105) TGFbeta coreceptor ENG

ESCSSEA-3 (stage-specific embryonic antigen)

glycolipid antigen

ESCSSEA-4 (state-specific embryonic antigen)

glycolipid antigen

ESC GCT343 karatan sulfate antigen

ESC GCTM2 karatan sulfate antigen

ESC Embryonic stem cell

ESC, ASC, ERC, BSC, GSC CD9 Cell adhesion, motility CD9

ESC, HSC, MSC, ERC, GSC Thy-1 (CD90) GPI-anchored cell surface protein, adhesion THY1 M7898 Monoclonal Anti-Thy 1.1

ESC, MSC, BSC tissue nonspecific alkaline phosphatase alpl

ESC, PSC Rex-1 zinc finger protein Zfp42

GSC Integrin alpha 6 Cell adhesion ITGA6

GSC Stra8 STRA8

GSC Germline stem cell

HSC CD38 Cell surface receptor, enzyme C1586 Monoclonal Anti-CD38

HSC CD45 Cell surface receptor, protein phosphatase PTPRC C7556 Monoclonal Anti-CD45

HSC CD34 Cell surface/cell adhesion CD34

HSC c-Kit (CD117 antigen) Stem Cell Factor receptor KIT K0131 Monoclonal Anti-c-kit/SCF Receptor antibody

HSC c-Kit (CD117 antigen) Stem Cell Factor receptor KIT C6740Anti-phospho-c-Kit (SCFR) (pTyr703) antibody produced in rabbit

HSC c-Kit (CD117 antigen) Stem Cell Factor receptor KIT C6615Anti-phospho-c-Kit (SCFR) (pTyr730) antibody produced in rabbit (1)

HSC c-Kit (CD117 antigen) Stem Cell Factor receptor KIT C6490Anti-phospho-c-Kit (SCFR) (pTyr823) antibody produced in rabbit (1)

HSC c-Kit (CD117 antigen) Stem Cell Factor receptor KIT C6365 Anti-phospho-c-Kit (SCFR) (pTyr936) antibody

HSC Sca-1

HSC Hematopoietic stem cell

LSC CD96 CD96

Stem Cell Marker Table



General Stem

Cell Biology

Category Marker Function/Pathway Gene PN SA AntibodyLSC Cytokeratin 18 Epithelial intermediate filament protein Krt18 C1399 Monoclonal Anti-Cytokeratin Peptide 18

LSC Cytokeratin 18 Epithelial intermediate filament protein Krt18 C8541 Monoclonal Anti-Cytokeratin Peptide 18

LSC Hepatic/biliary stem cells

MPC Desmin cytoskeletal protein DES D8281 Anti-Desmin

MSC HLA class I Antigen processing/presentationHLA-A, HLA-B, HLA-C

H1650 Monoclonal Anti-HLA Class I Antigen

MSC CD11b (Integrin alpha M) Cell adhesion ITGAM C0551 Monoclonal Anti-CD11b

MSC CD59 Cell adhesion CD59

MSC Mesenchymal (stromal) stem cell

MSC, ERC CD44 Cell adhesion (hyaluronic acid receptor) CD44 C7923 Monoclonal Anti-CD44

NPC beta-III Tubulin microtubule protein TUBB3 T2200 Anti-β-Tubulin III

NPC beta-III Tubulin microtubule protein TUBB3 T8660 Monoclonal Anti-β-Tubulin III

NPC beta-III Tubulin microtubule protein TUBB3 T5076 Monoclonal Anti-β-Tubulin Isotype III

NPC Microtubule Associated Protein 2 microtubule protein MAP2 M2320 Monoclonal Anti-MAP2 (2a+2b)

NPC Microtubule Associated Protein 2 microtubule protein MAP2 M9942 Monoclonal Anti-MAP2

NPC Musashi 1 RNA binding MSU1

NSC vimentin Intermediate filament protein VIM V4630 Anti-Vimentin

NSC vimentin Intermediate filament protein VIM V6389 Monoclonal Anti-Vimentin

NSC vimentin Intermediate filament protein VIM V5255 Monoclonal Anti-Vimentin

NSC Nestin NES

NSC Neural stem cell

NSC A2B5 Cell surface ganglioside marker A8229 Monoclonal Anti-A2B5

NSC, GSC, MSC Integrin beta1 (CD29) Cell surface adhesion factor ITGB1 I7533 Anti-phospho-Integrin β1 (pSer785)

NSC, ESC, PSC, HSC, CSC CD133/Prominin 1 PROM1

OLPC Oligodendrocyte Marker O4 cell surface protein O7139 Monoclonal Anti-Oligodendrocyte Marker O4

OLPC Galactosylceramide mylelin membrane lipid GALC G9152 Anti-Galactocerebroside

PESC Prostate endothelial stem cell

PESC, ECS, EGC, GC Oct4 Maintains pluripotency POU5F1 P0873 Anti-POU5F1 (Oct4)

PSC Nanog Maintains pluripotency NANOG N3038 Monoclonal Anti-Nanog

PSC SOX2 SOX2

PSC, EGC, EC Tra-1-60 karatan sulfate antigen

PSC, EGC, EC Tra-1-81 karatan sulfate antigen

RPEC Retinal pigment epithelium

RPC, OPC MITF Transcription factor MITF M6065 Monoclonal Anti-Microphthalmia antibody

SC Stem cell

SMPC Smooth muscle myosin contractile protein MYH11 M7786 Monoclonal Anti-Myosin (Smooth)

SMPC Calponin, basic cytoskeletal protein CNN1 C6047 Monoclonal Anti-Calponin

SMPC Actin, smooth muscle alpha cytoskeletal/contractile protein ACTA2 A5228 Monoclonal Anti-Actin, α-Smooth Muscle

Stem Cell Marker Table

Key:

AGPC Astroglia Progenitor Cell

ASC Adipose Stromal Cell

BDPC Bile Duct Progenitor Cell

BSC Blood Stem Cell

CM Cardiomyocyte

EB Embryoid Body

EC Tetracarcinoma Stem Cells

EGC Embryonic Germ Cells

ERC Endometrial Regenerative Cell

ESC Embryonic Stem Cells

GC Germ Cells

HSC Hematopoietic Stem Cell

LSC Leukemic Stem Cell

MPC Mesoderm Progenitor Cell

MSC Mesenchymal Stem Cell

NPC Neural Progenitor Cell

NSC Neural Stem Cell

OLPC Oligodendrocyte Progenitor Cells

OPC Osteoclast Progenitor Cell

PESC Pluripotent Embryonic Stem Cell

PESC Prostate Endothelial Stem Cells

PSC Pluripotent Stem Cell

RPC Retinal Progenitor Cell

RPEC Retinal pigment Epithelilial cell

SMPC Smooth Muscle Progenitor Cell



Gen

eral

Ste

m C

ell B

iolo

gy

Glycolipid Antigens of Mouse and Human EC and ES Cells

Antigen Oligosaccharide structure

Globoseries glycolipids

SSEA3 Galβ1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer

SSEA3, SSEA4 NeuNAcα 3Galβ1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer

Forssman GalNAcα1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer

Latoctoseries glycolipids

SSEA1

Galβ1 → 4GlcNAcβ1 → 4Galβ1 → 4Glcβ1 → Cer 3

Fucαl

Ganglioseries glycolipids

9-O-acetyl GD3 (ME 311) (9-O-acetyl) NeuNAcα2 → 8NeuNAcα2 → 3Galβ1 → 4Glcβ1 → Cer

GT3 (A2B5) NeuNAcα2 → 8NeuNAcα2 → 8NeuNAcα2 → 3Galβ1 → 4Glcβ1 → Cer


All of these glycosphingolipid antigens are synthesized by extension from a common precursor, lactosylceramide. The likely parts of these structures comprising the epitopes recognized by the different antibodies are highlighted. The enzymes responsible for the addi-tion of the third sugar residues (galactosyl-, glucosaminyl-, and sialyltransferase) appear to regulate the rate-limiting step that controls which synthetic pathway predominates. Human EC and ES cells are characterized by expression of the glycolipid antigens SSEA3 and SSEA4, and not the lactoseries antigen SSEAl (Lex). By contrast, mouse EC and ES cells are typically characterized by their expression of SSEAl. Mouse cells also express the globoseries Forssman antigen, which is not synthesized by human cells. Thus, despite the ap-parent distinction between mouse and human EC and ES cells in terms of their terminal sugars that comprise the different epitopes, the undifferentiated cells of both species do seem to share in common the expression of globoseries core structures.

Differential Expression of Characteristic Surface Marker Antigens of Mouse and Human EC and ES Cells

Antigen Mouse Human Comments

Forssman Yes No Globoseries glycolipid

SSEA 1 (Lex) Yes No Lactoseries glycolipid

SSEA3 No Yes Globoseries glycolipid

SSEA4 No Yes Globoseries glycolipid

TRA-1-60 No Yes Keratan Sulfate

TRA-1-81 No Yes Keratan Sulfate

GCTM2 No Yes Keratan Sulfate

Liver alkaline phosphatase (L-ALP) Yes Yes No antibody is available for murine ALP, but human L-ALP is rec-ognized by antibodies TRA-2-54 and TRA-2-49.

MRC Class 1 No Yes H-2 in the mouse; HLA in humans


→



General Stem

Cell Biology

Common Antibodies Used to Detect Antigens Expressed by Human EC and ES Cellsiso

MC631 SSEA3 Rat IgM Undifferentiated hES-EC

MC480 SSEA1 (Lex) Mouse IgM Differentiated hES-EC; includ-ing trophoblast

A2B5 GT3 Mouse IgM Neural

ME311 9-O-acetylGD3 Mouse IgG Neural

VINI556 GD3 Mouse IgM Neural

VIN2PB22 GD2 Mouse IgM Neural

MC8 1 3-70 SSEA4 Mouse IgG Undifferentiated hES-EC

TRA-1-60 TRA-1-60 Mouse IgM Undifferentiated hES-EC

TRA-1-81 TRA-1-81 Mouse IgM Undifferentiated hES-EC

TRA-2-54 L-ALP Mouse IgG Undifferentiated hES-EC

TRA-2-49 L-ALP Mouse IgG Undifferentiated hES-EC

TRA-1-85 Ok[a] Mouse IgG Panhuman

BBM1 β2-microglobulin Mouse IgG HLA

W6/32 HIA-A, -B, -C Mouse IgG HLA


Isolate!

Sigma’s MirPremier® microRNA Isolation Kit –Higher Purity miRNA, 50% faster with no detectable large RNA

mirPremier microRNA Isolation Kit provides a rapid and effiecient method for purifying and enriching miRNAs and other small RNAs directly from cells or tissues as rapidly and simply as a total RNA preparation.

n Optimized for purification of miRNAs and other small RNAs directly from diverse biological sources

n Rapidly purify and enrich miRNA in 30 minutes for downstream applications

n Higher-purity miRNA with no detectable large RNA

n No hazardous organic extractions

More rapid, more pure, less hands-on. To learn more, visit sigma.com/mirepremierMirPremier is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.


http://www.sigma-aldrich.com/mirepremier?cm_mmc=social-_-scribd-_-gen%20stem%20cell%20bio-_-pdf



Gen

eral

Ste

m C

ell B

iolo

gy

ES Marker Mouse Human

Alk Phos POS POS

BCRP-1 POS POS

CX43 POS POS

CX45 POS POS

FGF-4 POS POS

FOXD3 POS NEG

gp130 POS POS

LIFR POS NEG

Nanog POS POS

OCT 3/4 POS POS

REX-1 POS POS

SOX 2 POS POS

SSEA-1 POS NEG

SSEA-3 NEG POS

SSEA-4 NEG POS

STAT3 POS POS

TERT POS POS

TRA-1-60 NEG POS

TRA-1-81 NEG POS

UTF-1 POS POS

ES Line NIH Code Karyotype AC133 Alk Phos CD90 Cripto Cryopreservation Feeder-free GCTM-2 GDF3 Genesis hTERT Nanog OCT4 REX-1 SOX 2 SSEA-3 SSEA-4 TG343 TRA-1-60 TRA-1-81 UTF-1

H1 WA01 XY + + + + + + + + + + + + + + +

H7 WA07 XX + + + + + + + + + + + +

H9 WA09 XX + + + + + + + + + + + + + +

HES-1 ES01 XX + + + + + + + + + + + +

HES-2 ES02 XX + + + + + + + + + + + +

HES-3 ES03 XX + + + + + + + + + + + +

HES-4 ES04 XY + + + + + + + + + + + +

HES-6 ES06 XY + + + + + + + + + + +

HSF-6 UC06 XX + +

hESBGN.01 BG01 XY + + + + + + + + + +

hESBGN.02 BG02 XY + + + + + + + + + +

Miz-hES-1 MI01 XY + + + / - + + +

1995, First non-human primate ES line

2000, NIH issue guidelines for Federal funding of ESC research


2003, Ohio establishes the Center for Stem Cell and Regenerative Medicine


2001, Federal Government announces restrictions on funding for Stem Cell Research

2004, CA and NJ appropriate funding at the state level to support SC Research

2007, ES-like cells derived from skin fibroblasts

2003 - 2007, IL, MD, CT, IN, and other states pass legislation for state funding

1981, ES Cell First isolated in mice

1998, First human ES line



General Stem

Cell Biology

ES Line NIH Code Karyotype AC133 Alk Phos CD90 Cripto Cryopreservation Feeder-free GCTM-2 GDF3 Genesis hTERT Nanog OCT4 REX-1 SOX 2 SSEA-3 SSEA-4 TG343 TRA-1-60 TRA-1-81 UTF-1

H1 WA01 XY + + + + + + + + + + + + + + +

H7 WA07 XX + + + + + + + + + + + +

H9 WA09 XX + + + + + + + + + + + + + +

HES-1 ES01 XX + + + + + + + + + + + +

HES-2 ES02 XX + + + + + + + + + + + +

HES-3 ES03 XX + + + + + + + + + + + +

HES-4 ES04 XY + + + + + + + + + + + +

HES-6 ES06 XY + + + + + + + + + + +

HSF-6 UC06 XX + +

hESBGN.01 BG01 XY + + + + + + + + + +

hESBGN.02 BG02 XY + + + + + + + + + +

Miz-hES-1 MI01 XY + + + / - + + +

1995, First non-human primate ES line





2001, Federal Government announces restrictions on funding for Stem Cell Research

2004, CA and NJ appropriate funding at the state level to support SC Research

2007, ES-like cells derived from skin fibroblasts

2003 - 2007, IL, MD, CT, IN, and other states pass legislation for state funding

1981, ES Cell First isolated in mice

1998, First human ES line



Gen

eral

Ste

m C

ell B

iolo

gy

Unraveling and exploring the mysteries of life have been driving forces behind scientific studies since Aristotle first attempted to understand the origins of life. Through the eyes of the microscope, cellular processes have been observed, investigated, understood, and manipulated. This quest has intensified with the discovery of embryonic and adult stem cells. In recent years the genetic modification of stems cells, and the latest discovery, the transformation of somatic cells into stem cells have emerged as primary areas of therapeutic biomedical research. Potentially, stem cells can be transformed into any of the over 200 specialized cells in the body. Their use in cell-based therapies, drug development, and new disease models is still in its infancy. The future potential in basic and applied clinical research is fueled by over 2,000 articles published yearly.

Since the 1950s, adult stem cells have been used in bone marrow transplants and to develop a variety of treatments for diseases. Bone marrow transplants are used to treat malignant diseases such as acute and chronic leukemias, Hodgkin's disease and non-Hodgkin's lymphoma, myelodysplasia, multiple myeloma, myelofibrosis, and renal cell carcinoma. Increasingly, success has been seen in the treatment other diseases including aplastic anemia, sickle cell anemia, immune deficiency disorders, and autoimmune diseases such as scleroderma and multiple sclerosis. It was not until 1963 that the first qualitative description of bone marrow cell’s self-renewing activities by Ernest McCulloch and James Till, Ontario Cancer Institute, established the concept of stem cells.1 The first embryonic stem cells derived directly from the blastocyst in culture was reported by Martin Evans and Matthew Kaufman, University of Cambridge, in 1981.2 During the 1980s and 1990s, techniques for targeting and altering genetic material and in growing human cells in the laboratory flourished and laid the groundwork for human stem cell research. It was observed that telomerase activity is expressed in unspecialized stem cells and progenitor cells, but suppressed in somatic cells, and specific cell surface antigens SSEA-3, SSEA-4, Tra-1-60 and Tra-1-81 can be used to identify stem cells.

The first cloning of a mammal by somatic cell nuclear transfer (SCNT) in 1997 by Ian Wilmut, Roslin Institute, demonstrated mammalian differentiated cells can be transformed to an undifferentiated state by trans-acting factors present in the oocyte. This was followed by the search for factors that could mediate similar reprogramming of somatic cells.3 The following year, James Thomson, University of Wisconsin – Madison, used trans-acting factors to reprogram isolated cells from the inner cell mass of early embryos, leading to the development of the first human stem cell lines and John Gearhart, Johns Hopkins University, derived human embryonic germ cells from primordial germ cells.4,5 Pluripotent stem cell lines were then prepared from both. Subsequent research has yielded a better understanding of the characteristics, growth, and differentiating properties of adult and embryonic stem cells. Researchers are now beginning to identify the transcription factors necessary to reprogram human somatic cells to induced pluripotent stem cells.

The reprogramming and induction of human somatic cells to stem cells that exhibit the characteristics of embryonic stem cells have been pursued by many laboratories and was reported in separate papers by Shinya Yamanaka, Kyoto University, and James Thomson, University of Wisconsin – Madison, in 2007. Their findings were based on the similarity to human embryonic stem cells in morphology, proliferation mechanisms, surface antigens, pluripotent cell-specific gene epigenetic status, and telomerase activity. Yamanaka utilized Oct3/4, Sox2, Kif4, and c-Myc transcription factors to reprogram human dermal fibroblast cells. In this method, c-Myc and Klf4 may be involved in modifying chromatin structures so that Oct3/4 and Sox2 bind to their targets. Thomson transduced IMR90 fibroblast and postnatal fibroblast cells with a combination of Oct4, Sox2, Lin28, and NANOG. Lin28 and NANOG have been shown to improve cloning efficiency and recovery. The induced pluripotent human stem cells by both methods maintained their ability to differentiate into all three germ layers in vitro.

One potential limitation of the Yamanaka method using the c-Myc factor would be mutation through viral integration. The altered or forced expression of c-Myc may result in unwanted differentiation of the induced pluripotent stem cells. By using different transcription factors to reprogram and generate cells having the same characteristics as stem cells, the viability of this technology has been validated as a method to induce somatic cells. The induction process and how to harness it is now under further investigation.

Paralleling this achievement, research groups have continued to make progress in developing methods to improve the efficiency of human therapeutic cloning via SCNT. Advances have been made in the preparation of recipient cytoplasts, preparation and transfer of donor DNA, and parthenogenic activation of embryonic development. Recently, Andrew French, Stemagen Corporation, has been able to clone blastocysts from somatic cells.8 The ability to prepare nuclear transfer stem cells by this method is promising to regenerative medicine and cell-based drug discovery.

The preparation of human induced pluripontent stem cells is a milestone in stem cell biotechnology as is its impact on medical applications. This opens up the possibility of using them as well as embryonic stems cells in treating medical conditions such as cancer and birth defects, along with generating tissue for arthritis, burns, diabetes, heart disease, strokes, spinal cord injury, and disabilities like Alzheimer’s and Parkinson’s disease. Currently hematopoietic stem cells are the only stem cells being used in the treatment. Human induced pluripotent stem cells may have a role in drug development for efficacy or toxicity screening; thus, eliminating the need for animal testing and shortening the time to commercialization. They open the window for personalized medical treatment with cell-based therapies based on stem cells that contain the genetic makeup or genetically engineered DNA of the patient.

Stem Cell BiologyWhere we’ve been and where we’re heading!By: Dr. Greg Wall, PhD, BioChemsultants, LLC.



General Stem

Cell Biology

The study of embryonic stem cells will continue to further our understanding of how they transform. Both private and government funding of embryonic stem cell research will be critical to reach this goal to provide a platform for the future. Human induced stems cells eliminate the need to use human embryos; thus, minimizing or even eliminating cultural, political, religious, and ethical concerns. The commercial and social impact to be gained by stem cell research coming to fruition is immeasurable. The ability to use stem cells instead of scalpels will affect how we practice medicine and approach personalized healthcare. As our understanding of genomics and proteomics has enriched our appreciation of the complexities of life, our exploration of stem cells will solve even more of its mysteries.

References

1. Becker, A.J., et al., Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells. Nature, 197, 452-454 (1963).

2. Evans, M.J., and Kaufman, M.H., Establishment in culture of pluripotential cells from mouse embryos. Nature, 292, 154-156 (1981).

3. Wilmut, I., et al., Viable offspring derived from fetal and adult mammalian cells. Nature, 385, 810-813 (1997).

4. Thompson, J.A., et al., Embryonic Stem Cell Lines Derived from Human Blastocysts. Science, 282, 1145-1147 (1998).

5. Gearhart, J., et al., Derivation of pluripotent stem cells from cultured human primordial germ cells. P.N.A.S., 95, 13726-13731 (1998).

6. Yamanaka, S., et al., Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 131, 861-872 (2007)

7. Thomson, J.A., et al., Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science, 318, 1917-1920 (2007).

8. French, A.J., et al., Development of Human cloned Blastocysts Following Somatic Cell Nuclear Transfer (SCNT) with Adult Fibroblast. Stem Cells published online Jan 17, 2008, http://stemcells.alphapharmedpress.org/cgi/reprint/2007-0252v1.pdf

9. Dr. Greg Wall, Ph.D., BioChemSultants, LLC

Stem Cell BiologyWhere we’ve been and where we’re heading!By: Dr. Greg Wall, PhD, BioChemsultants, LLC.

The information contained below is a compilation of various publications. For your convenience Sigma product numbers have been added where applicable, however these products have not been tested in stem cell applications. Culture media formulation for ES (Embryonics Stem) and MEF Mouse Embryonic Fibroblast) feeder cells For 500 ml of ES complete media:

100 ml fetal bovine serum (F2442) 5 ml 100x non-essential amino acids, NEAA (M7145) 5 ml 100 x Pen-Strep (P4333 or P0781)5 ml 100 x sodium pyruvate (S8636 or P4562)5 ml beta-mercaptoethanol Stock solution = 3.5 ul diluted to 5 ml neat beta-maerpac-toethanol (M7522) with tissue culture grade water (W3500) - make fresh, do not store diluted solution 380 ml DME, high glucose (D5796) [See M3942 for reduced serum MegaCell formulation] 1 ml 106 Units/ml LIF (L5158)

For positive and negative selections, add: 5 ml 100x G418 (G8169)0.1 ml 5000x Ganciclovir ( use G2536 at 10 mM in PBS, P5368) Filter-sterilize as needed, store media at 4˚ C, pre-warm to 37 degrees C before use.

2x Freezing media preparation20% fetal bovine serum (F2442)20% DMSO (D2650)60% ES cell media Store freezing media at -20 degrees C and thaw to 37 de-grees C or room temperature before use. Ready-to-Go Freezing mediaSerum-free cyropreservation medium (C6295) Contains MEM, 8.7% DMSO, and methylcelluloseDMSO-free cryopreservation medium (C6039) Contains MEM, glycerol, calf and fetal bovine serumStandard cryopreservation medium (C6164 Contains MEM, DMSO, calf and fetal bovine serumComplete media for mouse embryonic fibroblast (MEF) feeder cells

For 500 ml media: 445 ml DMEM (D5796)50 ml fetal bovine serum (F2442) 5 ml 100x Pen-Strep (P4333 or P0781)Gelatin-treated culture ware All dishes/plates need to be treated with 0.2% gelatin for 5 minutes at room temperature. 0.2% gelatin is made from diluting 2% gelatin solution (G1393) with PBS (P5368) and autoclave to sterilize. The volume depends on culture vehicle, plates, slides, etc. It is necessary to cover the entire culture surface.

Protocols for Stem Cell CultureStem and feeder cell culture and cryopreservation



Gen

eral

Ste

m C

ell B

iolo

gy

Expansion and irradiation of MEF feeder cells n Plate one vial primary MEF cells in one T75 flask with 20 ml

MEF media. n Expand 1x T75 flask of cells into 1x T175 flasks. n Split 1x T175 flask of cells into 3 T175 flasks. n Split 3x T175 to 6xT175 flasks if more feeders are needed. n Trypsinize and pool all cells, spin down and resuspend cells in

50 ml MEF media. n Irradiate cells (gamma irradiation at 3000 rads). n Spin down and resuspend cells in MEF media. n Add an equal volume of 2x Freeze media. n Mix well and freeze for overnight in 0.5 ml aliquots at

-80 degrees C. n Store in liquid nitrogen.

Each T175 flasks of cells can be aliquoted into 6 vials. Each vial can then be plated onto 1x T25 flask. You may need to adjust these numbers according to plating efficiency.

Routine maintenance of ES cells To maintain a healthy and undifferentiated culture, cells need to grow at high density (above 106 to 107/ 8cm2 or one well of a 6-well plate) and they should be passaged frequently. As a general rule, the media should be changed daily and cells should be passed every other day with a 5 fold split. Cells are oftens ‘shocked’ when coming out of freeze and may show a lag time from initial culture set up to the first passage, perhaps 3 to 4 days.

Tips: Do a visual check of ES cultures. Observe for nesting (cells are in contact with each other). Undifferentiated ES cells should appear as compact colonies with prominent nucleoli.

When cells are colonial or ‘nested’ individual cell borders should not be distinguishable but the colony should have a well de-fined boundary and may appear a little refractile under a phase microscope.

To separate ES cells from MEF’s plate co-cultures on non-coated plates for 30 minutes at 37 degrees C. Feeder cells will attached leaving a relatively pure ES cell population in suspension.

To pass ES cells: n Aspirate media off the plate and rinse once with PBS. n Trypsinize cells with 0.05% trypsin (T3924) at 37 degrees C

for 3 to 5 minutes. n Add 5 ml of ES media and pipet vigorously to get single cell

suspension. n Spin at 1000 rpm for 5 minutes. n Plate cells out onto irradiated feeder cells.

Freezing ES cells on 96-well plates Aspirate media and wash once with PBS.

Freeze down the 3 x gelatinized only plate at -20 degrees C for DNA prep.

For the 2 x plates with feeder cells, add 25 ul trypsin STV and incubate at 37 degrees C for 5 minutes.

Disaggregate cells with 100 ul ES media and spin cells at 1K for 5 minutes.

Take off supernatant. Be careful not to suck cells from bottom.

Resuspend cells in 75 ul ES media.

Add 75 ul 2x freezing media.

Cover plates with parafilm, place plates in plastic bags and then in Styrofoam box and put in -80 degrees C freezer.

Thawing clones n Prepare feeder cells (50 ul /well) on 96-well plates. n Remove plates from -80 degrees C and let plates sit in warm

bath. n After clones are thawed spin plates at 1K for 5 minutes. n Remove supernatant from well. n Resuspend clone in 100 ul ES media and add clone to fresh

well with feeder cells. n After cells grow to confluence pass cells to 1 well of 24-well

plate. References

1. Stanford Transgenic Reesearch Center – online protocols

2. Nagy Lab, Samuel Lunenfeld, Mount Sinai Hospital – online protocols

3. Darwin Transgenic Mouse Core Facility, Baylor College of Medicine – online protocols

4. Comparative and Functional Genomics Dept., Max Planck Institute – online protocols

Stem and feeder cell culture and cryopreservation



General Stem

Cell Biology

Freezing hES CellsMany of the established hES cells have low recovery upon thawing, as low as 0.1 to 1%. This may be because of the method of passaging the cells. Mechanical picking or using collagenase dispersion usually results in large cell aggregates, which presumably do not get cryopreserved as efficiently as smaller clumps. Trypsinized cells in our lab have a recovery rate of about 10 to 20% or higher and do not require more compli-cated procedures such as vitrification (Figure 38-9).

Freezing MediumThe best recovery rate was observed in freezing medium con-sisting of 90% FBS-10% dimethyl sulfoxide (DMSO). However, Oct-4 expression in the thawed cells was lower than in cells fro-zen in hES cell growth medium with 10% DMSO. Nevertheless, by the next passage, the expression and distribution of Oct-4 and other markers of undifferentiated cells were indistinguish-able between these two freezing conditions. We routinely use the 90% FBS-10% DMSO medium.

Freezing ProtocolSelect a high-quality confluent culture with good morphology for freezing. We also recommend taking a picture of a sample field and staining for molecular markers characteristic of undif-ferentiated hES cells for future reference.

Materials needed:n Chilled freezing medium: 90% FBS, 10% DMSOn Cryovials, labeled with the line, passage number, and daten Cryovial rack (rack with ice reservoir by Corning)(Sigma Cat.

No. CLS430525)n Styrofoam rack from packaging for 15 ml centrifuge tubesn -80 °C freezer

1. Trypsinize the cells; centrifuge in PMEF medium (see earlier explanation).

2. Resuspend the pellet in the cold freezing medium. We rec-ommend freezing one confluent 35 mm plate per vial in 0.5 ml of freezing medium. Work quickly and keep the cells on ice after the addition of the freezing medium.

3. Aliquot cell suspension into prechilled freezing vials and sandwich the vials between two Styrofoam racks: tape to prevent the two racks from separating and transfer to a –80 °C freezer overnight. Transfer the cryovials to liquid nitrogen for long-term storage.

Thawing hES CellsThawing hES cells is a relatively simple procedure. The main rule to follow is to do everything quickly.

Preparation1. Prepare mitomycin C-treated PMEFs a day before thawing.2. Make a thawing medium. We use 70% hES cell growth

medium supplemented with 2x hLIF and 8 ng/ml bFGF with

30% hES cell- or PMEF-conditioned medium.3. Change the medium on the PMEF plate to the thawing

medium; equilibrate in the CO2 incubator for one hour. For 35 mm plates, use 1.5 ml medium; for four-well plates, use 0.5 ml medium per well.

4. Prepare a 50 ml conical tube with 10 to 15 ml of warm hES cell growth medium.

Thawing1. Thaw the vial in a 37 °C water bath, constantly agitating

while ensuring that the neck of the vial is above the water level. Check the content of the vial after about 40 seconds and at 10-second intervals until only a small piece of ice remains.

2. Quickly spray the vial with 70% isopropanol, then using a 1 ml pipetteman*, add warm hES cell medium to the contents of the vial dropwise with gentle agitation. Do it quickly but very gently. Immediately transfer the contents into the prepared 50 ml tube with warm hES cell medium; centrifuge at 160 x g for 5 minutes.

3. Remove the medium completely without touching the pellet.4. Add 0.5 ml of hES cell thawing medium, gently resuspend

the cells using a 1 ml pipetteman (2 to 4 repetitions), and transfer to prepared PMEF plates with equilibrated hES cell-thawing medium. Spread the cells evenly throughout the well by moving the plate several times in two directions at 90 degrees to each other; avoid swirling.

5. Check the cells the next day; if there are many dead cells or the medium has changed color, change two-thirds of the medium; otherwise, do not change it for another day.

6. The colonies usually begin to appear in 3 to 4 days and can be ready for splitting in 5 to 10 days (Figure 38-7).

hES Cell Quality ControlAlthough the morphology of hES cells is often used for evaluat-ing the quality of the culture and its readiness for passaging or freezing, this criterion alone cannot be used for an assessment of ES cell pluripotency. Staining for the expression of Oct-4 or alkaline phosphatase even in colonies of "perfect" morphol-ogy can result in one or both of these markers appearing in the cells only at the periphery of the colony. It is important, there-fore, to regularly assess the cells by analyzing the expression of markers of pluripotent cells. We look at Oct-4, SSEA-3, SSEA-4, TRA 1-60, and TRA 1-81 by immunostaining, or we perform an enzyme assay for alkaline phosphatase. The procedures for such assays and available antibodies are described elsewhere.

*adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006

Mouse ES cell freeze/thaw protocolsStem and feeder cell culture and cryopreservation



Gen

eral

Ste

m C

ell B

iolo

gy

Serum-Free and Specialty Media

Classical Media

Sera

Balanced Salt Mixtures

Reagents and Supplements

ECACC Cell Lines Labware

Technical Information

Cell Culture Manual2008-2009, 3rd Edition

Thinking Neuroscience?Think Sigma!

Show Seminars

Cell Biology

Antibodies

RNAi

Cell Culture

Custom Services

GENOMICS

FUNCTIONAL GENOMICS & RNAi

CELL BIOLOGY

PROTEOMICS

METABOLOMICS

PRODUCTS FOR

Life ScienceResearch2008-2009

2008-2009

ANTIBODIES

ANTIBIOTICS

CELL CULTURE

ENZYMES,SUBSTRATES,INHIBITORS

RNAi TOOLS

CUSTOMPRODUCTS

REAGENTS

UNITED STATESUS $

WebOrder/Customer Service sigma-aldrich.com/order Technical Service sigma-aldrich.com/techinfo

PhoneOrder/Customer Service 800-325-3010 Technical Service 800-325-5832

FaxOrder/Customer Service 800-325-5052 Technical Service 800-240-4668

MailSigma-Aldrich Corporation P.O. Box 14508 St. Louis, MO 63178 USA

Cell Culture ManualThe new 2008–2009 Sigma Cell Culture Manual, 3rd Edition, containing:Extensive technical information, protocols and formulas Complete line of application-tested cell culture products

The most comprehensive Cell Culture Manual ever!To request your copy go to sigma-aldrich.com/ccmanual

Antibody Catalog2007-2008 Antibodies CatalogThe catalog includes over 4,000 high quality monoclonals,polyclonals, secondaries and conjugates.

Also featured: n Hundreds of new antibodies n Application photos n Associated gene symbols n Technical appendix

To request your copy go to sigma-aldrich.com/antibodies

Neuroscience CatalogThe new catalog will present products useful in all areas of neuroscience research, including:

The 2008 - 2009 Sigma Life Science Catalogn 2000+ New Products – supporting Genomics, Functional Genomics, Cell Biology, Proteomics, and

Metabolomicsn New! Life Science Web Tool Guide – learn about the optimal online tool for finding the products

and technical information you needn Introducing the Sigma BioUltra grade – setting the quality standard for Life Science research – High-purity, high-activity BioUltra proteins with complete documentation – Well-defined, high-purity BioUltra reagents with consistent certification testingn Over 34,000 Laboratory Reagents and Biochemicals to support your research – antibodies,

antibiotics, cell culture media & reagents, enzymes, substrates, inhibitors, RNAi tools, Labware, and custom products

n Agonists and Antagonists to Neurotransmitter Receptors

n Ion Channel Modulators n Antibodies to Receptors, Ion Channels and

Transporters n Neuroproteins and Neuroenzymes n Neurotoxins n Neuropeptides n RNAi Libraries

n PCR/Amplification Reagents n Cloning Systems n Transfection Reagents n Antibody Arrays n Cell Culture Media, Supplements and Growth

Factors n Tools for Alzheimer’s Disease Researchn Centrally Acting Drugs

Additional Resources


http://www.sigma-aldrich.com/antibodies?cm_mmc=social-_-scribd-_-gen%20stem%20cell%20bio-_-pdf

http://www.sigma-aldrich.com/ccmanual?cm_mmc=social-_-scribd-_-gen%20stem%20cell%20bio-_-pdf

Sigma-AldrichStem Cell Biology Platform!

Infinite Expansion, Infinite Potential!

Sigma-Aldrich is pleased to announce the latest and most comprehensive selection of products for stem cell research! This platform is comprised of products necessary to the stem cell workflow from isolation and cryopreservation to in-vivo tracking with all of the related profiling tools in between!

Please visit sigma-aldrich.com/stembio to view our complete portfolio!

In addition to our product profiles you will also find the latest references, application notes, and a new Bulletin Board feature that will enable stem cell scientists like you to consult and exchange ideas and information!

Our Innovation, Your Research — Shaping the Future of Life Science

Our NEW Stem Cell Biology Platform begins with our comprehensive web site! Easily find products and detailed usage information in the following stem cell areas:

Isolation

Expansion

Characterization

Differentiation

Functional Profiling

In-vivo tracking and

Much More!

sigma-aldrich.com



http://www.sigma-aldrich.com/stembio?cm_mmc=social-_-scribd-_-gen%20stem%20cell%20bio-_-pdf


Accelerating Customers’

Success through Leadership

in Life Science, High

Technology and Service

KJE03670-5047600058

World Headquarters3050 Spruce St., St. Louis, MO 63103(314) 771-5765sigma-aldrich.com

Order/Customer Service (800) 325-3010 • Fax (800) 325-5052

Technical Service (800) 325-5832 • sigma-aldrich.com/techservice

Development/Bulk Manufacturing Inquiries (800) 244-1173

©2008 Sigma-Aldrich Co. All rights reserved. SIGMA, , SAFC, , SIGMA-ALDRICH, ALDRICH, , FLUKA, , and SUPELCO, are trademarks belonging to Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P. Hybri-Max, Accuspin, GenElute, and Director are trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Histopaque and MISSION are register trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Ficoll is a registered trademark of GE Healthcare. QuikEase is a trademark of Gene Therapy Systems, Inc. LONG is a trademark of GroPep Limited. BioPorter is a registered trademark of Gene Therapy Systems, Inc. Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip.

ArgentinaSIGMA-ALDRICH DE ARGENTINA S.A. Free Tel: 0810 888 7446 Tel: (+54) 11 4556 1472 Fax: (+54) 11 4552 1698

AustraliaSIGMA-ALDRICH PTY LTD. Free Tel: 1800 800 097 Free Fax: 1800 800 096 Tel: (+61) 2 9841 0555 Fax: (+61) 2 9841 0500

AustriaSIGMA-ALDRICH HANDELS GmbH Tel: (+43) 1 605 81 90 Fax: (+43) 1 605 81 20

BelgiumSIGMA-ALDRICH NV/SA.Free Tel: 0800 14747 Free Fax: 0800 14745 Tel: (+32) 3 899 13 01 Fax: (+32) 3 899 13 11

BrazilSIGMA-ALDRICH BRASIL LTDA.Free Tel: 0800 701 7425 Tel: (+55) 11 3732 3100 Fax: (+55) 11 5522 9895

CanadaSIGMA-ALDRICH CANADA LTD.Free Tel: 1800 565 1400 Free Fax: 1800 265 3858 Tel: (+1) 905 829 9500 Fax: (+1) 905 829 9292

ChinaSIGMA-ALDRICH (SHANGHAI) TRADING CO. LTD.Free Tel: 800 819 3336 Tel: (+86) 21 6141 5566 Fax: (+86) 21 6141 5567

Czech RepublicSIGMA-ALDRICH spol. s r. o. Tel: (+420) 246 003 200 Fax: (+420) 246 003 291

DenmarkSIGMA-ALDRICH DENMARK A/S Tel: (+45) 43 56 59 10 Fax: (+45) 43 56 59 05

FinlandSIGMA-ALDRICH FINLAND OYTel: (+358) 9 350 9250 Fax: (+358) 9 350 92555

FranceSIGMA-ALDRICH CHIMIE S.à.r.l. Free Tel: 0800 211 408Free Fax: 0800 031 052Tel: (+33) 474 82 28 00 Fax: (+33) 474 95 68 08

GermanySIGMA-ALDRICH CHEMIE GmbHFree Tel: 0800 51 55 000 Free Fax: 0800 64 90 000 Tel: (+49) 89 6513 0 Fax: (+49) 89 6513 1160

GreeceSIGMA-ALDRICH (O.M.) LTD.Tel: (+30) 210 994 8010 Fax: (+30) 210 994 3831

HungarySIGMA-ALDRICH Kft Ingyenes zöld telefon: 06 80 355 355 Ingyenes zöld fax: 06 80 344 344 Tel: (+36) 1 235 9055 Fax: (+36) 1 235 9050

IndiaSIGMA-ALDRICH CHEMICALS PRIVATE LIMITED Telephone Bangalore: (+91) 80 6621 9600 New Delhi: (+91) 11 4165 4255 Mumbai: (+91) 22 2570 2364 Hyderabad: (+91) 40 4015 5488 Fax Bangalore: (+91) 80 6621 9650 New Delhi: (+91) 11 4165 4266 Mumbai: (+91) 22 2579 7589 Hyderabad: (+91) 40 4015 5466

IrelandSIGMA-ALDRICH IRELAND LTD. Free Tel: 1800 200 888 Free Fax: 1800 600 222 Tel: (+353) 1 404 1900 Fax: (+353) 1 404 1910

IsraelSIGMA-ALDRICH ISRAEL LTD.Free Tel: 1 800 70 2222 Tel: (+972) 8 948 4100 Fax: (+972) 8 948 4200

ItalySIGMA-ALDRICH S.r.l. Numero Verde: 800 827018 Tel: (+39) 02 3341 7310 Fax: (+39) 02 3801 0737

Japan

SIGMA-ALDRICH JAPAN K.K.

Tel: (+81) 3 5796 7300

Fax: (+81) 3 5796 7315

Korea

SIGMA-ALDRICH KOREA

Free Tel: (+82) 80 023 7111

Free Fax: (+82) 80 023 8111

Tel: (+82) 31 329 9000

Fax: (+82) 31 329 9090

Malaysia

SIGMA-ALDRICH (M) SDN. BHD

Tel: (+60) 3 5635 3321

Fax: (+60) 3 5635 4116

Mexico

SIGMA-ALDRICH QUÍMICA, S.A. de C.V.

Free Tel: 01 800 007 5300

Free Fax: 01 800 712 9920

Tel: 52 722 276 1600

Fax: 52 722 276 1601

The Netherlands

SIGMA-ALDRICH CHEMIE BV

Free Tel: 0800 022 9088

Free Fax: 0800 022 9089

Tel: (+31) 78 620 5411

Fax: (+31) 78 620 5421

New Zealand

SIGMA-ALDRICH NEW ZEALAND LTD.

Free Tel: 0800 936 666

Free Fax: 0800 937 777

Tel: (+61) 2 9841 0555

Fax: (+61) 2 9841 0500

Norway

SIGMA-ALDRICH NORWAY AS

Tel: (+47) 23 17 60 60

Fax: (+47) 23 17 60 50

Poland

SIGMA-ALDRICH Sp. z o.o.

Tel: (+48) 61 829 01 00

Fax: (+48) 61 829 01 20

Portugal

SIGMA-ALDRICH QUÍMICA, S.A.

Free Tel: 800 202 180

Free Fax: 800 202 178

Tel: (+351) 21 924 2555

Fax: (+351) 21 924 2610

RussiaSIGMA-ALDRICH RUS, LLC Tel: +7 (495) 621 6037 +7 (495) 621 5828 Fax: +7 (495) 621 5923

SingaporeSIGMA-ALDRICH PTE. LTD.Tel: (+65) 6779 1200 Fax: (+65) 6779 1822

South AfricaSIGMA-ALDRICH SOUTH AFRICA (PTY) LTD.Free Tel: 0800 1100 75 Free Fax: 0800 1100 79 Tel: (+27) 11 979 1188 Fax: (+27) 11 979 1119

SpainSIGMA-ALDRICH QUÍMICA, S.A.Free Tel: 900 101 376 Free Fax: 900 102 028 Tel: (+34) 91 661 99 77 Fax: (+34) 91 661 96 42

SwedenSIGMA-ALDRICH SWEDEN ABTel: (+46) 8 742 4200 Fax: (+46) 8 742 4243

SwitzerlandSIGMA-ALDRICH CHEMIE GmbH Free Tel: 0800 80 00 80 Free Fax: 0800 80 00 81 Tel: (+41) 81 755 2828 Fax: (+41) 81 755 2815

United KingdomSIGMA-ALDRICH COMPANY LTD.Free Tel: 0800 717 181 Free Fax: 0800 378 785 Tel: (+44) 1747 833 000 Fax: (+44) 1747 833 313 SAFC (UK) Free Tel: 01202 712305

United StatesSIGMA-ALDRICH P.O. Box 14508 St. Louis, Missouri 63178 Toll-Free: 800 325 3010 Toll-Free Fax: 800 325 5052 Call Collect: (+1) 314 771 5750 Tel: (+1) 314 771 5765 Fax: (+1) 314 771 5757

Internet sigma-aldrich.com


http://www.sigmaaldrich.com/techservice?cm_mmc=social-_-scribd-_-gen%20stem%20cell%20bio-_-pdf

general stem cell biology brochure

Documents