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GeneralCatalog20112012

AppliChem GmbHOttoweg 4D-64291 DarmstadtGermanyPhone +49 6151 9357-0Fax +49 6151 9357-11eMail service@de.applichem.comwww.applichem.com

AppliChem ScandinaviaLyngby Storcenter 66, lejl. 1203DK-2800 Kongens LyngbyDenmarkPhone +45 39 64 46 66Fax +45 39 64 34 81eMail service@dk.applichem.com

AppliChem UK LtdEnterprise House58B Main StreetHigh BenthamLancasterLA2 7HYUnited KingdomPhone +44 15242 63391Fax +44 15242 63052eMail info@uk.applichem.com

AppliChem Asia Pte Ltd8 Boon Lay Way #03-10TradeHub 21Singapore 609964Phone +65 65154616Fax +65 65150220eMail service@sg.applichem.com

AppliChem Inc.2814 Mary Avenue63144 St. LouisMissouri, USAPhone +1 314 968-3331Fax +1 314 968-5006eMail service@us.applichem.com

GeneralCatalog I

Your local distributor

GeneralCatalog 20112012

II GeneralCatalog

DearColleague,Having informationatyourfinger-

tipswheneveryouneed it isoneof

themajor foundationsofproductive

work.Ournewheavyweight

theAppliChemCatalog20112012

claimsnothing less than tosimplify

your labwork.

Even though the digital age provides lots of newpossibilities, we think that there is still no sub-stitute for a printed work that provides valuablecontent. This enables you to always have pro-duct information ready at hand. Of course, youwill learn all the news and trends from our web-site, a continually updated resource about ourchemistry and capabilities.

A lot of things were accomplished last year.We set the course for the future changes thatwill benefit you. We established a new productinformation management system and im-plemented the eCl@ss classification current-ly our database holds 9000 different chemicalsubstances. In addition, we planned new logi-stic facilities, further optimized manufacturingprocesses and implemented the GHS guidelines.

We hope you enjoy browsing through theAppliChem GeneralCatalog 20112012. Pleasealso view our website www.applichem.com which is always worth a visit.

Yours sincerelyAppliChem

GeneralCatalog III

Recombinant Proteins bioconfident grade.What does that mean? Typically, growth factors and cytokines areproduced in bacteria, yeast or other eukaryotic expression systems(e.g. insect cells, CHO cells). The organism selected for production,as well as the reagents for cultivation (components of media andadditives) bear the risk of contamination by bacteria, viruses andTSE. Other components thereof, such as endogenously producedenzymes and other proteins, may contaminate the protein ofinterest. The endotoxin level of bacterially expressed growth factorsis particularly difficult to minimize. bioconfident grade reliablebiologically safe: A newly developed expression system for recom-binant proteins of animal origin uses the barley grain endotoxin-free, antibiotic-free, serum-free, no endogenous growth factors,but natural protein folding and glycosylation.

Colorada A New Generation of FluorescentLabels. The Colorada product line is a selection of the newestfluorescent labels available today. Dyestructures were carefully chosen to pro-vide top-notch brightness performances,improving bioanalytical assays to thehighest level. Eight different basicstructures with precise absorption andemission maxima were selected to coverthe full working range of wavelengths,starting from 400 nm up to 850 nm. TheColorada structures are based on 4 diffe-rent chemical classes: coumarin, dipyrro-methene borondifluoride, acenaph-thopyrrole and cyanine.

AppliCoat Plate Stabilizer Improving Quality & Stability of ELISA. Usingready-to-use ELISA kits from manufacturers is easy and con-venient. Sometimes however, home-made ELISA are required,perhaps because there is no kit available with the right antibodiesor characteristics, such as the limits of detection are not appro-priate. Ready-to-use ELISA kits from reputable suppliers may bestored for two years at 4C without any problem. With home-madeELISA kits it is a completely different story. For any new measure-ment one has to coat a new plate, because after storage for somedays the plates dont perform as well as before. In commercialELISA kit production, the plates are not only blocked after coating,but also stabilised. This easy-to-perform process has been an in-dustry standard for thirty years. For stabilisation of a plate one hasto incubate with a coating stabiliser solution. It is just as simple asa second blocking step. However there were no such high qualitystabiliser solutions freely available in low volumes for use in theresearch lab until now. AppliChem now offers a product for use inevery research lab in volumes starting as small as 50 ml, which iscalled the AppliCoat Plate Stabiliser (Cat. No. A7708).

Cell Proliferation Test Kit XTT. Cell proliferation as-says are widely used in cell biology for the study of growth factors,cytokines or media components. They are also applied in the scree-ning of cytotoxic agents and lymphocyte activation. In order todetermine the number of viable cells Cell Proliferation Kit XTTemploys 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetra-zolium-5-carboxanilide salt (XTT). It is only in living cells thatmitochondria are capable of reducing XTT to form an orangecolored water soluble dye. Therefore, the concentration of the dye isproportional to the number of metabolically active cells. This testreplaces the radioactive assays.

Size Exclusion Chromatography. Size-exclusionchromatography (SEC) is a popular method to separate biomole-cules based on their size. Primarily, it is applied to the separation ofbiopolymers such as proteins and nucleic acids, i.e. water-solublepolymers. This system is also called gel filtration, typically with

beads of dextran or agarose serving as gelmatrix. Smaller molecules pass signi-ficantly slower through the column thanlarger molecules. Not to be confused withgel electrophoresis, there are big diffe-rences in terms of the separation prin-ciple. SEC does not require electric currentand the sieving effect will not separatesmall molecules first. AppliChems size-exclusion chromatography columns arepacked with AppliXchange-G25 M, a bea-ded composite material composed par-tially of polymerized dextran. It exhibitshigh selectivity, high resolution andchemical stability. Typical applications are

desalting, buffer exchange, purification of proteins and nucleicacids, and dye terminator removal.

SeparateIT. Improving Separation During Electrophoresis:SeparateIT gels represent a novel gel matrix for DNA electrophore-sis. Gel polymers are arranged in a conceptually different way, inaccordance with a new theoretical model of gel electrophoresis.SeparateIT gels selectively retard the migration of large molecules,so that DNA bands remain sharp but are more spread out relative toeach other. Thanks to this increased spacing, the resolving power ofSeparateIT gels is at least twice that of any other gels, includingpolyacrylamide gels. Two DNA fragments in the 70-150 bp rangethat differ by 1 bp can be separated on SeparateIT gels that are 8 cmlong.

ExitusPlus, now even more active DNA decontaminationas effective as bleach, but nonhazardous. We further developed theformulations of all liquid ExitusPlus reagents, resulting in adecontamination activity as high as bleach. The new reagentscontain some alcohol (< 5 %) for improving the access of theactive components to the surface, and a reduced concentration ofcolor indicator.

NewProducts

AppliChem ships most bioche-micals at ambient temperaturethat have to be stored at either2-8C or 20C. This includesproducts like Albumin, Pro-teinase K, X-Gal, IPTG or liqui-fied Phenol. We know fromexperience that refrigeratedshipment of these products isnotnecessary, sincewecouldntobserve a reduction in quality or activity of theproducts under these conditions, even if theshipment took several days.For longterm storage please place such productseither in a refrigerator or in a freezer uponarrival, as indicated on the package label/bottle.Shipments with dry ice will have an extra dryice charge of 23.80 for shipments outsideGermany. In case temperature sensitive productshave been ordered together with insensitive pro-ducts, we keep the right to send them separatelyby courier to guarantee the quality of theproducts.

Ordering Boxes

In terms of safety and opti-mization of packing and ship-ment it is of advantage whencertain bottled products arepacked in boxes of 6 pieces(1L) and 4 pieces (2.5L).Therefore, the price per piecein a box normally is lower than

the price of a single bottle. Ordering odd quan-tities, e.g. 5 or 7 pieces, will raise the costs forpacking dramatically. Thus, calculation is asfollows: the maximum numbers of boxes will becharged at the box price, additional bottles willbe charged at the single bottle price.

IV GeneralCatalog

ShippingInformation

DextraSEC

Oligonucleotide Purification

Conductivity

Elution volume [mL]

Elution volume [mL]

Elution volume [mL]

Absorption

Absorption

Absorption

pH

Oligo Desalting from 0.8 MNaCl

Removal of Rhodamine fromOligonucleotide Labelling

Removal of ammonia(33%NH3) fromNucleic acid

MaximumPerformanceSize Exclusion Chromatography (Gel Filtration)

Applications, e.g.

dye terminator removal in automated

DNA sequencing procedures

sharp peaks

more than 90% recovery

easy-to-use

high resolution

high chemical stability

DextraSEC

VI GeneralCatalog

UniqueDNA-ExiusPlusTheonlydecontaminationsolution thateffectivelydestroysnucleicacids.non-corrosivenon-hazardousbiodegradableready-to-usesolution foruse inPCRworkplacesdecontaminatesworkingsurface