gene sen2 perspectives for using in tetraploid potato breeding€¦ · perspectives for using in...

Gene Sen2 –perspectives for using in

tetraploid potato breeding

JAROSŁAW PLICH

I H A R - P I B

M Ł O C H Ó W R E S E A R C H C E N T E R , P O L A N D

Synchytrium endobioticum (Schilberszky) Percival

Yield losses: 50-100%

Soil contamination (spores viable > 40 year)

Quarantine pathogen

Resistant potato cultivars

Resistance against S. endobioticum

Pathotype 1(D1)• Major locus Sen1 on chromosome XI

(Hehl et al. 1999)

• Major locus Sen1-IV chromosome IV (Brugmans et al. 2006)

~40 virulentpathotypes

• QTLs on chromosomes I, IX (Ballvora et al. 2011)

• QTLs on chromosomes I, II, VI, VIII, VIII, X, XI (Groth et al. 2013)

• Locus on chr. XI + QTLs on all potato chromosomes (Obidiegwu et al. 2015)

• Locus on chromosome XI – pathotypes 6, 18 (Bartkiewicz et al. 2018)

• Sen3 - chromosome XI – pathotypes 2, 6, (18)(Prodhomme et al. 2019)

• Sen2 – chromosome XI – broad spectrum resistance (Plich et al.2018)

Potato clone

DG 97-264resistant against eight pathotypes:

1 (D1) 2 (G1) 6 (O1) 8 (F1) 18 (T1) 2 (Ch1) 3 (M1) 39 (P1)

Gene Sen2 –broad-spectrum resistance

Most important in Europe Identified in Poland

Gene Sen2 in diploid potato progeny

DG 97-264

DG 97-1805

Gene Sen2 in diploid potato progeny

Sen2

Gene Sen2 –genomic localization

Gene Sen2 – introducing into tetraploid potato genepool

DG 97-264Resistant to 8 pathotypes

1(D1) 2(G1) 6(O1) 8(F1) 18(T1) 2(Ch1) 3(M1) 39(P1)

DG 01-591Resistant to 8 pathotypes, 2n gamets

1(D1) 2(G1) 6(O1) 8(F1) 18(T1) 2(Ch1) 3(M1) 39(P1)

4x – 2x


DG 01-591Resistant to 8 pathotypes, 2n gamets

1(D1) 2(G1) 6(O1) 8(F1) 18(T1) 2(Ch1) 3(M1) 39(P1)


4x – 2x

4x – 4x

R - 33 R - 33 R - 33

R - 33 R - 33

R - 33 R - 33 R - 33

DG

97

-26

4

DG

97

-18

05

An

cest

or

1

An

cest

or

2

DG

01

-59

1

M 6

2 8

19

11

-10

-48

Deo

dar

a

Tom

ensa

SEN 14-01 i 14-02

R R R S S S S

5450_3 (TasI)

2502_1(HinfI)

32_502

MappingpopulationSEN 12-01

DG 97-264

DG 97-1805

Gene Sen2 – DNA markers

DG

97

-26

4

DG

97

-18

05

An

cest

or

1

An

cest

or

2

DG

01

-59

1

M 6

2 8

19

11

-10

-48

Deo

dar

a

Tom

ensa

SEN 14-01 i 14-02

R R R S S S S

5450_3 (TasI)

2502_1(HinfI)

32_502

Gene Sen2 – DNA markers

Is gene Sen2 present in tetraploid potato cultivars?

Cultivar/clone Resistance to (some) virulent pathotypes

Markers for Sen2 Y1del AAT1 Kc81032 Markers for Sen33

DG 97-264 1 1 1 0

DG 01-591 1 1 1 0

R-I-3 1 1 1 0

Adam 1 0 1 1 1

Antares 1 0 0 0 0

Apollo 1 0 0 0 0

Bałtyk 1 0 0 0

Bzura 1 0 1 1 1

Cekin 1 0 1 1 1

Gandawa 1 0 1 1 1

Gawin 1 0 1 1 1

Giewont 1 0 0 0 0

Ibis 1 0 1 1 1

1 –Obidiegwu et al. 2015; 2 – Bartkiewicz et al. 2018; 3- results from paper Prodhomme et al. 2019


Markers for Sen2 Y1del AAT Kc8103 Markers for Sen3

Igor 1 0 1 1

Ikar 1 0 1 1 1

Karolin 1 0 1 1 1

Kuba 1 0 1 1 1

Megusta 1 0 1 1

Miriam 1 0 1 0 0

Nicola 1 0 0 0

Ora 1 0 1 1 1

Otolia 1 0 0 ? 0

Palladia 1 0 1 1

Rudawa 1 0 1 1 1

Saphir 1 0 0 0 0

Zagłoba 1 0 1 1 1



Markers for Sen2 Y1del AAT Kc8103 Markers for Sen3

Ari 0 0 0 0

Bryza 0 0 0 0

Calrose 0 0 0 0

Carpatin 0 0 0 0

Cayuga 0 0 0 0

Dalila 0 0 0 0

Sarpo Mira 0 0 0 0

Sebago 0 0 0 0

Seneca 0 0 0 0

Wauseon 0 0 0 0


A new project: Sen2Breed

Using of Sen2 gene in breeding of new potato cultivars resistant to virulent pathotypes of Synchytrium endobioticum, causal agent of potato wart disease

The aim of Sen2Breed

▪ To show benefits of introduction of the gene Sen2 into new potato cultivars

▪ To present advantages of using DNA markers linked with Sen2 in breeding programs

▪ To develop advanced potato breeding lines possessing the gene Sen2 (single or in combination with other resistance sources e.g. Sen1, Sen3)

Take-home message

▪ Gene Sen2 was identified and mapped to potato chromosome XI

▪ Sen2 provides high level of resistance for broad spectrum of S. endobioticum pathotypes in diploid as well as in tetraploid potatoes

▪ Potato cultivars with Sen2 will available… soon (relatively soon ☺)

Acknowledgments

Jarosław Plich

Ewa Zimnoch-Guzowska

Jarosław Przetakiewicz

Beata Tatarowska

Bogdan Flis

Technical Staff

gene sen2 perspectives for using in tetraploid potato breeding€¦ · perspectives for using in...

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