gel doc xr+ with image lab
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8/14/2019 Gel Doc XR+ With Image Lab
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Eortless and Accurate
Gel Imaging and Analysis
Molecular ImagerGel DocXR+Systems
Imaging
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Fast, Automated, Reproducible Imagingor Perect Images Every Time
For more than two decades, the Molecular Imager
systems rom Bio-Rad have been widely recognized
and trusted high-quality imaging instruments. Whether
you perorm routine imaging o PCR products or protein
puriications, the Gel Doc XR+ imaging system will meet
your needs. Designed or ease o use and an ability
to support a wide array o applications, it is a perect
it or individual laboratories, multiuser core acilities,
or research on inectious diseases such as avian lu
and H1N1 lu. Quality standards o engineering and
manuacturing make the system adaptable to academic
or biopharmaceutical laboratories. Thousands o
researchers over the years have published Gel Doc XRsystem images in dozens o journals and established a
record o reliability and excellence or the system.
The Molecular Imager Gel Doc XR+ system enables
quick and easy visualization, documentation, and
analysis o nucleic acid and protein gels, blots, and
macroarrays with a ew clicks o the computer mouse.
It is a lexible and easy to use system that supports
luorescence and colorimetric detection methods. The
large imaging area accommodates a broad range o gel
sizes, rom large handcast polyacrylamide gels to small
ReadyAgarose gels. The Gel Doc XR+ system is the
ideal accompaniment to your PCR and electrophoresis
systems, enabling image analysis and documentation
o restriction digests, ampliied nucleic acids, and
genetic ingerprinting.
What the Gel Doc XR+ System Can Do for You:
Automated workflow from image to results
n Makes your gel imaging and analysis quick
and eortless
n Eliminates the need or training
n Allows any user to repeat the same worklow
in exactly the same way
Automated image capture
n Produces beautiul pictures o your gels
n Delivers quantitative analysis o your
protein and DNA samples
Automated image optimization
n Delivers image data that is always
optimized and reproducible
without imaging artiacts
Automated image analysis
n Produces reports with data
organized the way you want
n Minimizes time rom lab
work to presentations
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Gel Doc XR+ System Applications
Nucleic Acid Protein Gel
Electrophoresis Electrophoresis Blotting
Ethidium bromide Coomassie Blue Colorimetric
SYBR Green Copper stain Qdots 525
SYBR Sae Zinc stain Qdots 565
SYBR Gold Flamingo uorescent gel stain Qdots 625
GelGreen Oriole stain CY2
GelRed Silver stain Alexa Fluor 488
Fast Blast DNA stain Coomassie Fluor Orange DyLight 488
SYPRO Ruby Fluoresce in
Krypton
SYBR Safe stain
Oriole stain
Qdot blot
Coomassie Brilliant B
R-250 stain
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Gel Doc XR+ System Is Poweredwith Image Lab Sotware
Fast Results from a Completely
Automated Workflow
The Gel Doc XR+ system is controlled by Image
Lab sotware to automatically and reproducibly
generate gel images. Image Lab sotware is ast,
taking you rom gel to printed results in seconds.
Your results are visible with a single click o the
computer mouse.
Image Lab sotware eliminates the guesswork
in imaging. You wont have to perorm tedious
repetitive steps to ind the right ocus setting.
You wont have to guess which exposure
times will best visualize the bands o interest.
Image calculations and corrections are done
automatically or your application. Whether you
are working with protein gels, nucleic acid gels,
or your own customized imaging application,
the Gel Doc XR+ system will select the proper
settings or optimum detection conditions o the
stain or label.
You wont need to set aside time or training new
users; the system and the sotware are easy
to use and work together rom setup to inish.
Imaging and image analysis can become the
simplest work in the laboratory.
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Simple and Reproducible Image Capture
Place your gel on the Gel Doc imagers sample tray and
run your protocol: Your work is done in just one step.
Design and save protocols or the imaging steps in
your experiments, and Image Lab sotware will run theprotocols exactly the same way every time. The protocol
eature negates variability in results due to dierent people
operating the imaging system. When a new project is related
to an existing one, you can reuse the previously created
protocol by revising its parameters and renaming it. Image
Lab sotware lets you devote your valuable time to research
and discovery instead o to wondering i you used your
imaging system properly.
Results and Reports
In addition to printing a picture o your gel or your records, Imag
Lab sotware creates and prints reports o your experimental
data. Any part o the report can be copied into popular documen
processing applications such as Adobe Acrobat and Microsot
Word, PowerPoint, or Excel iles. To include a 3-D view o your
gel, copy it using the Snapshot tool, and paste it into your
presentation slide. High quality, good-looking reports are easy toproduce with the combined power o the Gel Doc XR+ system
and Image Lab sotware.
When an analysis parameter is changed, the results tables are
updated instantly to relect the new data.
Tutorials
With Image Lab sotware, you dont need previous imaging
experience to produce optimal gel images. Detailed tutorials are
accessible via the toolbar and start-up page to acquaint you with
all o the Image Lab sotware capabilities.
Lane proles depict band intensity and represent quantities o sample
components separated in a gel.
The image stays ocused at any zoom point.
Automated workfow or any application.
Three Unique Features
The power o Image Lab sotware stems rom three eatures
n Image Lab sotwares proprietary algorithms (patent pending
calibrate the system at setup or automatic control o ocus a
any zoom level and automatic correction o imaging artiacts
n Image Lab sotware perorms lat ielding corrections
speciically and consistently or each application
n Image Lab sotware saves the entire worklow (image capture
results, report) in a protocol ile. The protocols can be edited,
resaved, reused, and shared among multiple users
Tutorials or novice users.
Its that easy to use Image Lab to generate the data andreports that you need. Please look at the other tutorials ormore detailed explanations o other Image Lab eatures.
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Molecular Biology Applications
The Gel Doc XR+ system can be used in multiple
workows. Gel electrophoresis remains an important tool
or quantitative analysis o complex protein and nucleic
acid samples. Applications include nucleic acid isolationor amplifcation or molecular cloning techniques, nucleic
acid quality evalution prior to quantitative PCR, gene
silencing, gene modulation, and gene expression. DNA gel
electrophoresis is also used or fne separations o nucleic
acid ragments or entire plasmids or analysis, cloning,
transection, environmental testing, and quality assurance
testing. Gel Doc XR+ systems are suitable or laboratories
engaged in RNAi analysis, molecular diagnostics,
epigenetics, pharmacogenomics, and orensic testing o
genomic DNA.
Alternative to UV illumination to better preserve DNA samples. Top,
serial dilu tions o EtBr-stained precision molecular mass ruler (Bio-Rad
Laboratories, Inc.) on agarose gel imaged with UV light; bottom, serially
diluted precision molecular mass ruler stained with SYBR Sae stain on
agarose gel imaged with XcitaBlueconversion screen.There is no loss
in sensitivity when a combination o SYBR Sae nucleic acid uorescent
stain and less harmul blue excitation is used instead o UV-excitable EtBr.
The image o the gel stained with SYBR Sae stain was taken using the
XcitaBlue conversion screen and a flter or SYBR Sae stain and GFP.
Less harmul detection methods preserve samples better and more
efciently or downstream uses such as cloning.
Safe DNA Imaging
Acquire images o DNA electrophoresis samples
without exposing samples or users to harmul UV
radiation by adding the XcitaBlue conversion screen
to your Gel Doc XR+ system. The screen enablesdetection o DNA with SYBR Sae stain and other blue
lightexcitable uorescent stains such as GelGreen and
SYBR Green. Images and analyzed data produced
by Gel Doc XR+ systems are oten incorporated into
sample preparation and sample quantitation workows.
Fluorescence,RU
18,000
16,000
14.000
12,000
10,000
8,000
6,000
4,000
2,000
0
Sample load, ng
51.2 25.6 12.8 6.4 3.2 1.62 0.8 0.4 0.2 0.1
SYBR Sae andXcitaBlue conversionscreen
EtBr and UV light
Ethidium Bromide vs. SYBR Safe DNA Stain Detection
PFGE gel stained with EtBr.
E. coli colony screening by PCR.
ReadyAgarose 96 Plus gel stained with EtBr.
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Protein gels stained with silver stain (top) and Coomassie Blue
stain (bottom). Documentation o protein gels or lab notebooks or
sample analysis, including protein purity and concentration assessment
or dierential protein expression studies or to monitor gene modulation
products, is supported by the array o analys is tools in the Gel Doc XR+
system. The gel stained with silver stain shows salmon muscle, soybean,
and rat brain extracts, protein mixtures, andE. coli
extracts comparedwith Precision Plus Protein unstained standards.
Protein gels fuorescence-stained with Oriole stain (top) and
Flamingo fuorescent gel stain (bottom).Achieve higher levels
o sensitivity with uorescent stains to dierentiate proteins with low
expression. Resolve closely spaced spots or bands or protein profling,
quantitation, and characterization.
Capturing Gene Typing Results
Gel Doc XR+ systems are the imagers o choice or
pulsed ield gel electrophoresis (PFGE). Gel Doc XR+
system images generated rom CHEF Mapper XA
systems or PFGE have been used oten by PulseNet
International (a global network o health and oodregulatory agency laboratories coordinated by the
Centers or Disease Control and Prevention) or strain
typing and molecular epidemiology studies. Image
data and analysis o PFGE gels have contributed to
new indings in cancer research, ood saety, public
health, quality control, and genome mapping.
Images produced by the Gel Doc XR+ system have contributed
to new ndings in many elds.This gel shows a strain oSalmonella
identifed in a ood saety experiment.
Molecular ImagerGel DocXR+System
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Life ScienceGroup
09-0689 1009 Sig 0308Bulletin 5838 Rev B US/EG
Bio-RadLaboratories, Inc.
Web site www.bio-rad.com USA 800 4BIORAD Australia 61 02 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil55 21 3237 9400Canada 905 364 3435 China 86 21 6426 0808 Czech Republic420 241 430 532 Denmark44 52 10 00 Finland09 804 22 00 France 01 47 95 69 65Germany089 318 84 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary36 1 455 8800 India 91 124 4029300 Israel03 963 6050Italy39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666 New Zealand0508 805 500Norway23 38 41 30 Poland48 22 331 99 99 Portugal351 21 472 7700 Russia 7 495 721 14 04 Singapore 65 6415 3188 South Africa 27 861 246 723Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland061 717 95 55 Taiwan 886 2 2578 7189 United Kingdom 020 8328 2000
Adobe Acrobat is a trademark o Adobe Systems Incorporated. Coomassie is a trademark o BASF Aktiengesellschat. Excel and Microsot are
trademarks o Microsot Corporation. GelGreen is a trademark o Biotium, Inc. SYBR is a trademark o Invitrogen Corporation. CHEF (U.S. patent
5,549,796, issued to Stanord University) is exclusively licensed to Bio-Rad Laboratories, Inc.
Specifcations
Automation Capabilities
Workow automated selection Application driven; user selected or recalled by a protocol
Workow automated execution Controlled by a protocol via application specifc setup or image area, illumination source, flter, analysis,
and reporting
Workow reproducibil ity 100% repeatabilit y via recallable protocols; rom image capture to quantitative analysis and reports
Autoocus (patent pending) Precalibrated ocus or any zoom setting or sample height
Image at felding** Dynamic; precalibrated and optimized or every application Autoexposure 2 user-defned modes (intense or aint bands)
Hardware Specications
Maximum sample size 28 x 36 cm
Maximum image area 25 x 26 cm
Excitation source Trans-UV and epi-white are standard (302 nm included, with 254 and 365 nm available as options);
optional trans white, sel-powered or conversion screen; optional XcitaBlue UV/blue conversion screen
Illumination control 3 modes (trans-UV, trans white, epi-white)
Detector CCD
CCD resolution (H x V) 1,392 x 1,040 pixels
Pixel size (H x V) 4.65 x 4.65 m
Cooling system Not applicable
Camera cooling temperature Not available
Filter holder 3 positions (2 or flters, 1 without flter)
Emission flters 1 included (standard), 3 optional
Dynamic range >3.0 orders o magnitude
Pixel density (gray levels) 4,096
Instrument size (L x W x H) 36 x 60 x 96 cmInstrument weight 32 kg
Operating Ranges
Operating voltage 110/115/230 V AC nominal
Operating temperature 1028C (21C recommended)
Operating humidity