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www.mascom-bremen.de GC-APPI Interface for Thermo Scientific LC-MS Instruments

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Page 1: GC-APPI Interface - images.mascom-bremen.deimages.mascom-bremen.de/GC-APPI.pdf · TECHNICAL OVERVIEW The MasCom GC-(APPI) interface for Thermo Scientific LC-MS systems allows you

www.mascom-bremen.de

GC-APPI Interface

for Thermo Scientific™

LC-MS Instruments

www.mascom-bremen.de

Page 2: GC-APPI Interface - images.mascom-bremen.deimages.mascom-bremen.de/GC-APPI.pdf · TECHNICAL OVERVIEW The MasCom GC-(APPI) interface for Thermo Scientific LC-MS systems allows you

Get the most out of your Orbitrap... ...by expanding it to GC-MS with the new MasCom GC-(APPI) interface

The MasCom GC-(APPI) interface design is fully compatible to all Xcalibur source housings. It allows you to switch from LC to GC operation within minutes. The entire GC-system (oven, transfer line, APPI ion source) is simply operated as a standard Xcalibur device and part of the instrument method. Together with the ease of use, the MasCom GC-(APPI) interface is a must for any analytical labo-ratory using Orbitrap MS.

www.mascom-bremen.de

Page 3: GC-APPI Interface - images.mascom-bremen.deimages.mascom-bremen.de/GC-APPI.pdf · TECHNICAL OVERVIEW The MasCom GC-(APPI) interface for Thermo Scientific LC-MS systems allows you

TECHNICAL OVERVIEWThe MasCom GC-(APPI) interface for Thermo Scientific LC-MS systems allows you to fully exploit the superb high-resolution performance of Orbitrap™ instruments for your GC analyses. Within minutes the change from standard LC-(ESI) to GC-(APPI) is done. After heating up the transfer line and interface to operating temperature you are ready for the first GC injection!High resolution and accurate mass with external calibration is now available for your GC-MS analyses!

FEATURES OF THE GC-APPI INTERFACE

• Swift change from LC-MS to GC-MS operation within minutes

• The MasCom GC-(APPI) interface hardware is fully compatible with current and previous LC Orbitrap systems

• Operation of the interface, transfer line and GC oven are seamlessly integrated into the Xcalibur data analysis software

• Integrated gas purifier system

• Widest range of ionizable com-pounds among all GC-(API)-MS methods available

GC-APPI Interfacefor Thermo Scientific™ LC-MS Instruments

Page 4: GC-APPI Interface - images.mascom-bremen.deimages.mascom-bremen.de/GC-APPI.pdf · TECHNICAL OVERVIEW The MasCom GC-(APPI) interface for Thermo Scientific LC-MS systems allows you

Diagram 1: Accurate mass spectra of Benzo(a)pyrene

Diagram 2: Measurement of 5 pg 13C12-2,3,7,8-TCDD on column with CS2 as dopant

Sampling rate: 10 Hz @ R= 10.000 (FWHM)

High resolution, speed, accuracy and dynamic range are fully available even by running a wide range of different compounds. Accurate mass‚ at your fingertips‘ is not a phrase anymore in GC-MS – it has become reality.

The high sampling rate of the Exactive™ with high resolution and accurate mass performance results in excellent chromatographic separation capabilities.

250.0 250.5 251.0 251.5 252.0 252.5 253.0 253.5 254.0 254.5 255.0 255.5 256.0m/z

0

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Rela

tive

Abun

danc

e

252.0931

253.0965

254.1003

Benzo(a)pyrene C22H12, M

+: 252.0934

7.90 7.95 8.00 8.05 8.10 8.15 8.20 8.25 8.30 8.35Time (min)

m/z

020406080

100

020406080

100AA: 381501

AA: 479303

331.0 332.0 333.0 334.0 335.0 336.0 337.0 338.00

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100 333.9330

331.9358

335.9301

337.9205

Relat

ive A

bund

ance

Relat

ive A

bund

ance

O

O Cl

Cl Cl

Cl

13C12-2,3,7,8-TCDD

The addition of a dopant can help increasing the sensitivity without sacrificing the chromatographic performance.

Page 5: GC-APPI Interface - images.mascom-bremen.deimages.mascom-bremen.de/GC-APPI.pdf · TECHNICAL OVERVIEW The MasCom GC-(APPI) interface for Thermo Scientific LC-MS systems allows you

Diagram 3: Selected ion chromatogram of EPA Mix 8270

High resolution and accurate mass available with external calibration are the key features of Orbitrap mass spectrometers. This technology has proven its value in LC-MS for almost ten years. Although the demand for the same performance in GC-MS has been voiced already many years ago, the MasCom GC-(APPI) interface for the first time enables these powerful capabilities of the Orbitrap systems for GC-MS analyses.

FEATURES OF THE GC-APPI IONIZATION METHOD

• Direct interaction of VUV photons with analytes = true single photon ionization with very little excess energy deposition in generated molecular ions

• Almost exclusive formation of intact molecular radical cation (M•+) with little or no fragmentation, in contrast to GC-(EI)-MS

• Ionization of a wide range of organic compounds including dioxins with very high sensitivity in the fg-range

• Better suited for the detection of small organic compounds as GC-(APCI)-MS

• Virtually no matrix effects, adduct formation, or other transformation reactions observed, in contrast to GC-(APCI)-MS

• The very high reproducibility of the GC chromatograms allows MS/MS analyses at pre-selected GC retention times leading to ultimately high selectivity for e.g. in targeted analyses. Structural infor-mation about unknown compounds in non-targeted analyses becomes readily available

• Optionally, very simple addition of dopants to the ion source chamber leads to strongly increased selecti-vity of the method towards com-pound classes; e.g. toluene (“soft” protonation) or CS2 (“soft” charge transfer)

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Relat

ive A

bund

ance

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100 4.63 5.766.79

6.16

5.28

5.09

6.17

6.01

6.847.94

5.15

9.048.84

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10.9710.95

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Time (min)

N H 2

N O 1

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N H 2 N O 2

Cl

C H 3

O HC H 3

N H

N H

N H 2

Cl

N H 2

Cl

Use your existing LC-MS Orbitrap instrument and expand its capabilities to high resolution GC-MS. With MasCom‘s new GC-(APPI) interface you are able to run a GC sample at any time on your LC-MS instrument.

Page 6: GC-APPI Interface - images.mascom-bremen.deimages.mascom-bremen.de/GC-APPI.pdf · TECHNICAL OVERVIEW The MasCom GC-(APPI) interface for Thermo Scientific LC-MS systems allows you

Get the most out of your Orbitrap...

MasCom Technologies GmbHSophie-Germain-Str. 4 · 28201 BremenPhone: +49 421-57297-0 · Fax: +49 421-571032E-Mail: [email protected]

MASCOM TECHNOLOGIESMasCom Technologies, located in Bremen, Ger-many, was founded in 1991 and has started with offering service and repair for high resolution mass spectrometer from Thermo Fisher (former Finnigan MAT). Today MasCom Technologies refurbishes and sells mass spectrometer and provides worldwide service and repair for ion trap MS, quadrupole MS, and high resolution GC-MS systems from Thermo Fisher. Almost exclusively MasCom offers service for the MAT95XL/XP instruments for more than 20 years. In 2000 the on-line shop for MS spare parts and consumables was opened and expanded until today with over 7000 different parts sold to more than 3000 custo-mers worldwide. For more than 15 years MasCom also manufactures secondary electron multipliers (SEM) and has become one of the world leading manufacturer for multipliers.

www.mascom-bremen.de