gadda cv (february 15, 2017) - georgia state university · giovanni gadda v.5 2016-2017 guest...

Giovanni Gadda V. �1

Name: Giovanni GaddaRank: Distinguished University ProfessorDepartment: ChemistryCollege of Arts & SciencesGeorgia State University

a) EDUCATION

1990-1995 Ph.D., Biochemistry, 1995, Dipartimento di Fisiologia e Biochimica Generali Università degli Studi di Milano, Milano, Italy, Thesis:” Study of the Active Site of D-Amino Acid Oxidase from Rhodotorula gracilis”, Advisor: Prof. Mirella Pilone Simonetta

1987-1989 Honors, Dipartimento di Fisiologia e Biochimica Generali Università degli Studi di Milano, Milano, Italy, Thesis: “Studies of Limited Proteolysis on Spinach Ferredoxin-NADP+ Reductase”, Advisor: Prof. Giuliana Zanetti

1984-1989 Laurea Summa cum Laude 110/110 laude (B.Sc.) in Biological Sciences Università degli Studi di Milano, Milano, Italy

b) PROFESSIONAL CREDENTIALS

2014- Distinguished University Professor (Tenured), Biochemistry and Enzymology, Departments of Chemistry and Biology, Georgia State University, Atlanta, Georgia (Date of appointment 08-14-2014)

2013- Appointed at the Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, Georgia

2012- Professor (Tenured), Biochemistry and Enzymology, Departments of Chemistry and Biology, Georgia State University, Atlanta, Georgia (Date of appointment: 08-14-2012)

2007-2012 Associate Professor (Tenured), Biochemistry and Enzymology, Departments of Chemistry and Biology, Georgia State University, Atlanta, Georgia (Date of appointment: 08-14-2007)

2008 Visiting Professor, Enzymology, Dipartimento di Scienze Biomolecolari e Biotecnologie, Università degli Studi di Milano, Milano, Italy (June 2008)

2000-2007 Assistant Professor, Biochemistry and Enzymology, Departments of Chemistry and Biology, Georgia State University, Atlanta, Georgia (Date of appointment: 09-14-2000)

2005 Visiting Professor, Enzymology, Dipartimento di Scienze Biomolecolari e Biotecnologie, Università degli Studi di Milano, Milano, Italy (March 2005)


1995-2000 Postdoctoral Research Associate, Laboratory of Prof. Paul F. Fitzpatrick, Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, Project: Mechanistic and Biochemical Studies of Nitroalkane Oxidase (From: 06-13-1995 to 09-13-2000)

1995 Postdoctoral Research Associate, European Molecular Biology Organization Short Term Fellowship, Laboratory of Prof. Sandro Ghisla, The University of Konstanz, Germany, Project: Biochemical and Kinetic Studies of Cholesterol Oxidase, (From: 02-01-1995 to 05-20-1995)

1993-1995 Graduate Teaching Assistant, Università degli Studi di Milano sede di Varese, Varese, Italy (now Università dell’ Insubria, Varese, Italy)

1989-1990 Research Assistant, Laboratory of Prof. Giuliana Zanetti, Dipartimento di Fisiologia e Biochimica Generali, Università degli Studi di Milano, Milano, Italy

c) SCHOLARSHIP AND PROFESSIONAL DEVELOPMENT

c.i) Grants/Awards/Honors

2015-2018 “Nitronate Monooxygenase: A Model for Flavosemiquinones in Oxidative Catalysis”; Giovanni Gadda, P.I.; National Science Foundation; NSF CHE-1506518; $540,000 ($358,803 in direct costs); from 07/01/2015 to 07/31/2018.

2014 “Mechanisms for Heme Uptake and Degradation: New Antibacterial Venues”; Giovanni Gadda, Irene Weber, Robert Harrison and Zehava Eichenbaum, P.I.’s’ Molecular Basis of Disease Area of Focus, Georgia State University; $15,000; from 01/01/2014 to 31/05/2014.

2013 “Southeast Enzyme Conference”; Giovanni Gadda, P.I.; National Science Foundation; NSF MCB-1316227; $2,000, awarded.

2011-2015 “Mechanistic and Structural Studies of Choline Oxidase”; Giovanni Gadda, P.I.; National Science Foundation; NSF MCB-1121695; $547,729 ($379,052 in direct costs), from 08/15/2011 to 07/31/2015.

2007 Georgia State University, Office of Educational Opportunity and TRIO Programs, 4th Annual Community Connections Salutes Dr. Giovanni Gadda, “For his continued support and dedication to the Ronald E. McNair Post-Baccalaureate Achievement Program”, November 7, 2007.

2007 Georgia State University Outstanding Faculty Achievement Award 2007.

2006-2012 "CAREER: Structure-Function Studies on Choline Oxidase"; Giovanni Gadda, P.I.; National Science Foundation; NSF MCB-0545712; $860,173 ($591,185 in direct costs), from 07/01/2006 to 05/31/2012.

2006 "Probing the Mechanism of 2-Nitropropane Dioxygenase: a Model for Flavin Semiquinone Intermediates in Catalysis"; Giovanni Gadda, P.I.; American


Chemical Society – Petroleum Research Fund – Type AC Grant; PRF 47363-AC; $80,000, from 02/01/06 to 08/31/08.

2003 “Biochemical and Mechanistic Investigation of Choline Oxidase, a Bacterial Enzyme Involved in Tolerance Towards Environmental Stress”; Giovanni Gadda, P.I., Dabney Dixon, Mentor; Faculty Mentoring Grant, GSU; $ 11,000. From 07/01/03 to 06/31/04.

2002 National Science Foundation Travel Grant to attend the 14th International Symposium on Flavins and Flavoproteins, University of Cambridge, Cambridge, UK.

2002 “Investigation of Carbon-Hydrogen Bond Cleavage of Primary Alcohols Catalyzed by Flavoprotein Oxidoreductases”; Giovanni Gadda, P.I.; American Chemical Society – Petroleum Research Fund – Type G Grant; PRF 37351-G4; $35,000, from 07/01/02 to 08/31/05.

2002 “Biophysical Investigation of Biomolecules Using Fluorescence Spectroscopy”; Giovanni Gadda, P.I., Collaborating Investigators: David D. Wilson, Dabney W. Dixon, Jenny Yang, and Emelita D. Breyer: Quality Improvement Funds, GSU; $24,955.

2001 “Biochemical and Mechanistic Studies of Flavin-Dependent Enzymes”; Giovanni Gadda, P.I.; Research Initiation Grant, GSU; $10,000/year, from 07/01/01 to 06/30/02.

2000 GSU Start-Up Funds: (09/14/00-06/30/02) $412,000; itemized in: supplies: $40,000; equipment $210,000; support for students: $135,000 (support for 3 Ph.D. students for 3 years at about $15,000/year); summer salary: 20% summer salary for 3 years at $9,000/year.

1999 National Science Foundation Travel Grant to attend the 13th International Symposium on Flavins and Flavoproteins, University of Konstanz, Germany.

1996 National Science Foundation Travel Grant to attend the 12th International Symposium on Flavins and Flavoproteins, University of Calgary, Canada.

1995 Travel Grant from the European Community FLAPS 1 Network to attend the First European Network Meeting at the University of Wageningen, The Netherlands.

1995 Baden-Wurttemberg-Lombardy Cooperation program to study the catalytic mechanism of cholesterol oxidase in the laboratory of Prof. S. Ghisla, University of Konstanz, Germany.

1995 European Molecular Biology Organization Short Term Fellowship to study the catalytic mechanism of cholesterol oxidase in the laboratory of Prof. S. Ghisla, University of Konstanz, Germany.


c.ii) Grants as Co-P.I.

2016 “Determining the Impact of Protein-Protein Interactions on Transglutaminase Regulation and Activity”: Giovanni Gadda, Co-P.I. (T. Zeczycki, P.I., East Carolina University, NC); National Science Foundation - Molecular Biophysics; $1,080,550, from 07/01/17 to 06/31/2021 - PENDING.

2016 “Determining the Impact of Protein-Protein Interactions on Transglutaminase Regulation and Activity”: Giovanni Gadda, Co-P.I. (T. Zeczycki, P.I., East Carolina University, NC); National Institutes of Health; $1,828,268, from 07/01/17 to 06/31/2021 - PENDING.

2013-2014 “Coatings for Indoor Air Quality”: Giovanni Gadda Sub-Contractor (Prime Award: Dow Chemicals, Inc.); $20,000; from 01/01/2013 to 12/31/2014.

2012 “Coatings for Indoor Air Quality”: Giovanni Gadda Sub-Contractor (Prime Award: Dow Chemicals, Inc.); $8,000; from 07/01/2012 to 06/30/2013.

2011 “Assigning Function to 2-Nitropropane Dioxygenase”; Giovanni Gadda Sub-Contractor (Prime Award: NIH/NIGMS Grant No. RC2 GM092602); $14,450 ($10,000 in direct costs); from 04/01/2011 to 03/31/2012.

2008 “Netzel Scholars: opportunity in Chemistry and Biology”; Giovanni Gadda, Co-P.I. (D. Dixon, P.I.); National Science Foundation STEM Program; $599,474, from 10/01/08 to 09/30/13.

2005 "Fluorescent Aptamers for Glycoprotein Detection"; Binghe Wang, P.I., Collaborating Investigators: Giovanni Gadda and Zhen Huang; NIH (CA113917), $291,000, 7/1/05-6/30/07.

2003 “Biomolecular interactions”; David D. Wilson, P.I., Collaborating Investigators: Stuart Allison, Emelita Breyer, Dabney Dixon, Giovanni Gadda, and Tom Netzel; Research Program Enhancement, GSU; $ 87,000 (cost sharing $ 12,000; total $ 99,000), from 07/01/03 to 06/31/04.

c.iii) Scholarship & Professional Recognition

2017 Strategic Planning Working Group for Panel Education, GSU College of Arts and Sciences, Member

2017- Editorial Board of the Journal of Biological Chemistry

2017 International Organizing Committee for the 19th International Symposium on Flavins and Flavoproteins, Gröningen, the Netherlands, July 2-6, 2017

2017 Panel Member for the NSF, Division of Chemistry, Panel Name: Enzymes and Metalloproteins

2017 On-site Organizer of the Eight Annual Southeast Enzyme Conference, Atlanta, April 8, 2017


2016-2017 Guest Editor with Dr. Pablo Sobrado, Virginia Tech, for the Special Issue of Archives of Biochemistry and Biophysics on Flavoproteins: Beyond the Classical Paradigms, to be published in Summer 2017.

2016-2017 Chair Faculty Search Committee for 2 Tenure Track Positions in the Chemistry Department of Georgia State University

2016-2019 Chair of the Chemistry Department Curriculum Committee at Georgia State University

2016-2019 Elected Member, Executive Committee of the Department of Chemistry, Georgia State University.

2016- Editorial Board of BBA - Proteins and Proteomics

2016 External Reviewer for Promotion & Tenure, Department of Chemistry, University of North Florida, Jacksonville, Florida

2016 External Reviewer for Promotion & Tenure, Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, Texas

2016 Panel Member for the NSF, Division of Molecular and Cellular Biosciences, Panel Name: Mechanistic Molecular Biophysics I (reviewed 78 grant applications)

2016 On-site Organizer of the Seventh Annual Southeast Enzyme Conference, Atlanta, April 16, 2016

2016 Committee Member, Distinguished University Professor Review Committee, Georgia State University

2016 External Reviewer for Promotion & Tenure, Department of Biology and Department of Chemistry, Pomona College, Claremont, California

2015-2018 Member of the Georgia State/Honors College National Scholarship Selection Committee

2015 Behind the Scenes Job Search Committee Workshop, Georgia State University

2015- Member of the Georgia State University Chemistry and Biology Curricula Collaboration with Nursing and Health Professions

2015 Co-Chair (elected) of the Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, July 2015

2015 External Reviewer (unique) for the Biochemistry, BS Program at Middle Tennessee State University, Murfreesboro, TN

2015 On-site Organizer of the Sixth Annual Southeast Enzyme Conference, Atlanta, April 11, 2015

2015 External Reviewer for Promotion & Tenure, Department of Chemistry, University of North Florida, Jacksonville, Florida


2015 External Reviewer for Promotion & Tenure, Department of Chemistry and Biochemistry, Auburn University, Auburn, Alabama

2014 External Reviewer for Promotion & Tenure, College of Arts and Sciences, University of Alabama, Tuscaloosa, Alabama

2014 Review Panel Member for National Science Foundation CHE/CLP

2014 Co-Vice Chair (elected) of the Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, July 2014

2014- Appointed Distinguished University Professor at Georgia State University

2014 Review Panel Member for NSF/CHE/CLP

2014 International Organizing Committee for the 18th International Symposium on Flavins and Flavoproteins, Thailand, July 27-August 1, 2014

2014 On-site Organizer of the Fifth Annual Southeast Enzyme Conference, Atlanta, April 14, 2014

2013 Guest Editor for the Special Issue of Archives of Biochemistry and Biophysics on Cofactor Assisted Enzymatic Catalysis, to be published in January 2014.

2013-2016 Elected Member of the Executive Committee of the Department of Chemistry, Georgia State University

2013- Appointed at the Center for Diagnostics and Therapeutics, Georgia State University

2013 On-site Organizer of the Fourth Annual Southeast Enzyme Conference, Atlanta, April 20, 2013

2012-2013 Editorial Board of ISRN Biochemistry

2012 On-site Organizer of the Third Annual Southeast Enzyme Conference, Atlanta, April 14, 2012

2011 On-site Organizer of the Second Southeast Enzyme Conference, Atlanta, April 2, 2011

2011 Organizing Committee Member, “Third International Conference on Cofactors”, Turku, Finland, July 10-15, 2011

2011 International Organizing Committee for the 17th International Symposium on Flavins and Flavoproteins, Berkeley, CA, July 24-29, 2011

2011 Third Annual Chemistry Department Poster Day

2011-2013 Elected Alternate Councilor of the Division of Biological Chemistry of the American Chemical Society

2010 Founder, Organizer and Program Chair of the First Southeast Enzyme Conference, Atlanta, April 10, 2010


2010 Herty 2011 Medal Award Selection Committee Member, ACS-GA Section

2010-2013 Editorial Board of International Review of Biophysical Chemistry (IREBIC)

2010 Second Annual Chemistry Department Poster Day

2009 Herty 2010 Medal Award Selection Committee Member, ACS-GA Section

2009 Guest Editor for the Special Issue of Archives of Biochemistry and Biophysics on Oxidative Enzymes, published in January 2010

2009 First Annual Chemistry Department Poster Day.

2009-2013 Editorial Board of Enzyme Research

2008 Herty 2009 Medal Award Selection Committee Member, ACS-GA Section.

2008-2013 Honorary Editorial Board of Biochemistry Insights

2008-2013 Editorial Board of Open Enzyme Inhibition Journal

2008 Organizing Committee Member, “Second Interdisciplinary Conference on Vitamins, Coenzymes, and Biofactors”, University of Georgia, Athens, GA, U.S.A., October 26-31, 2008

2007-2015 Director, Chemistry Department Graduate Program

2007-2015 Committee Member, College of Arts and Sciences Graduate Council

2007-2013 Georgia State University Institutional Biosafety Committee, Member

2007 Organizer, “Strategies in Enzymatic Oxidation Catalysis” Symposium, at the 234th National Meeting of the American Chemical Society, Boston, MA, U.S.A., August 19 to 23, 2007.

2006-2007 Chemistry Faculty in charge of the National Research Council Ph.D. Program Review for Georgia State University

2006-2007 Associate Director, Chemistry Department Graduate Program

2006-2012 Member of the GSU Institutional Biosafety Committee

2006-2011 Coordinator for the Chemistry Department Coordinator, Roland E. McNair Post-Baccalaureate Achievement Program

2005- Editorial Board of Archives of Biochemistry and Biophysics

2005 Content Reviewer for "Principles of Biochemistry", fourth edition, by H.R. Horton, L.A. Moran, K.G. Scrimgeour, M.D. Perry, and J.D. Rawn, Pearson Prentice Hall, New Jersey

2004-2007 Member of the Chemistry Department Petitions Committee

2003-2007 Chemistry Department Liaison for Georgia State University Library


2001-2006 Member of the Chemistry Department Curriculum Committee

2001- Ad hoc Reviewer for 46 different peer-reviewed scientific journals:

ACS CatalysisActa Crystallographica DAfrican Journal of BiotechnologyAnalytica Chimica ActaAngewandte ChemieAnnals of MicrobiologyApplied Biochemistry and BiotechnologyApplied and Environmental MicrobiologyApplied Microbiology and BiotechnologyArchives of Biochemistry and BiophysicsBiochemical Journal, BiochemieBiochemistryBiochimica Biophysica Acta Proteins and ProteomicsBioorganic ChemistryBioorganic & Medicinal ChemistryBiotechnology and Applied BiochemistryBMC BiochemistryBMC Plant BiologyCancer ResearchCatalysis CommunicationsCentral European Journal of ChemistryChemistry - A European JournalClinical Cancer ResearchComputational and Structural Biotechnology JournalCurrent MicrobiologyCurrent Pharmaceutical DesignEuropean Journal of BiochemistryFEBS LettersFEBS JournalFEMS LettersIRSN BiochemistryJournal of Agricultural and Food ChemistryJournal of the American Chemical SocietyJournal Biological ChemistryJournal of Molecular BiologyJournal of Molecular Catalysis B: EnzymaticJournal of Organic ChemistryInternational Journal of Biological MacromoleculesMicrobiology OpenNature Chemical BiologyOpen Enzyme Inhibition JournalOrganic LettersPLOS ONEStructureThe Journal of Physical ChemistryTrends in Biochemical Sciences


1999- Ad hoc Reviewer for 10 agencies:

National Science FoundationAmerican Chemical Society - PRFBiotechnology and Biological Sciences Research Council - UKAustrian Science FundJeffress Memorial TrustSouth Carolina Commission on Higher EducationPennsylvania Department of HealthUnited States-Israel Binational Science FoundationResearch CorporationNetherlands Organisation for Scientific Research

1994 Translation from English to Italian of the Textbook Principles and Techniques of Practical Biochemistry, Cambridge University Press,Cambridge, UK

1991 Habilitation to the Biology Italian National Board

c.iv) Affiliations to Professional Societies

American Society of Biochemistry and Molecular Biology (2001-)American Chemical Society (2001-)American Society for Microbiology (2003-)American Association for the Advancement of Science (1999-)

c.v) Peer-Reviewed Journal Articles (total: 88)

1. Gadda, G., Aliverti, A., Ronchi, S. and Zanetti, G. (1990) Structure-Function Relationship in Spinach Ferredoxin-NADP+ Reductase as Studied by Limited Proteolysis, J. Biol. Chem. 265, 11955-11959

2. Aliverti, A., Gadda, G., Ronchi, S. and Zanetti, G. (1991) Identification of Lysine 116 as the Target of N-ethylmaleimide Inactivation of Ferredoxin-NADP+ Oxidoreductase, Eur. J. Biochem. 198, 21-24

3. Gadda, G., Negri, A. and Pilone, M.S. (1994) Reaction of Phenylglyoxal with Arginine Groups in D-Amino Acid Oxidase from Rhodotorula gracilis, J. Biol. Chem. 269, 17809-17814

4. Gadda, G., Beretta, G.L. and Pilone, M. (1994) Chemical Modification of Lysyl Residues of Rhodotorula gracilis D-Amino Acid Oxidase, Biochem. Mol. Biol. Int. 33, 947-955

5. Gadda, G., Beretta, G.L. and Pilone, M.S. (1995) Reactivity of Histidyl Residues in D-Amino Acid Oxidase from Rhodotorula gracilis, FEBS Lett. 363, 307-310

6. Gadda, G., Edmondson, R.D., Russell, D.H. and Fitzpatrick, P.F. (1997) Identification of the Naturally Occurring Flavin of Nitroalkane Oxidase from Fusarium oxysporum as a 5-Nitrobutyl-1,5-dihydroflavin and Conversion of the Enzyme to the Active FAD-Containing Form, J. Biol. Chem. 272, 5563-5570


7. Edmondson, R.D., Gadda, G., Fitzpatrick, P.F. and Russell, D.H. (1997) Identification of Native Flavin Adducts from Fusarium oxysporum Using Accurate Mass Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry, Anal. Chem. 69, 2862-2865

8. Gadda, G., Wels, G., Pollegioni, L., Zucchelli, S., Ambrosius, D., Pilone, M.S. and Ghisla, S. (1997) Characterization of Cholesterol Oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum, Eur. J. Biochem. 250, 369-376

9. Gadda, G., and Fitzpatrick, P.F. (1998) Biochemical and Physical Characterization of the Active FAD-Containing Form of Nitroalkane Oxidase from Fusarium oxysporum, Biochemistry 37, 6154-6164

10. Gadda, G., and Fitzpatrick, P.F. (1999) Substrate Specificity of a Nitroalkane Oxidizing Enzyme, Arch. Biochem. Biophys. 363, 309-313

11. Gadda, G., Dangott, L.J., Johnson, W.H.Jr., Whitman, C.P., and Fitzpatrick, P.F. (1999) Characterization of 2-Oxo-3-Pentynoate as an Active-Site-Directed Inactivator of Flavoprotein Oxidases: Identification of Active-Site Peptides in Tryptophan 2-Monooxygenase, Biochemistry 38, 5822-5828

12. Pollegioni, L., Gadda, G., Ambrosius, D., Ghisla, S., and Pilone, M.S. (1999) Cholesterol Oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum: Effect of Surfactants and Organic Solvents on Activity, Biotechol. Appl. Biochem. 30, 27-30

13. Gadda, G., Banerjee, A., and Fitzpatrick, P.F. (2000) Identification of an Essential Tyrosine Residue in Nitroalkane Oxidase by Modification with Tetranitromethane, Biochemistry 39, 1162-1168

14. Gadda, G., and Fitzpatrick, P.F. (2000) Iso-Mechanism of Nitroalkane Oxidase: 1. Inhibition Studies and Activation by Imidazole, Biochemistry 39, 1400-1405

15. Gadda, G., and Fitzpatrick, P.F. (2000) Mechanism of Nitroalkane Oxidase: 2. pH and Kinetic Isotope Effects, Biochemistry 39, 1406-1410

16. Gadda, G., Choe, D.Y., and Fitzpatrick, P.F. (2000) Use of pH and Kinetic Isotope Effects to Dissect the Effects of Substrate Size on Binding and Catalysis by Nitroalkane Oxidase, Arch. Biochem. Biophys. 382, 138-144

17. Gadda, G., Banerjee, A., Dangott, L.J., and Fitzpatrick, P.F. (2000) Identification of a Cysteine Residue in the Active Site of Nitroalkane Oxidase by Modification with N-ethylmaleimide, J. Biol. Chem. 275, 31891-31895

18. Gadda, G., Banerjee, A., Fleming, G.S., and Fitzpatrick, P.F. (2001) Evidence for An Essential Arginine In the Active Site of Nitroalkane Oxidase, J. Enzyme Inhibition 16, 157-163

19. Daubner, S.C*, Gadda, G.*, Valley, M.P., and Fitzpatrick, P.F. (2002) Cloning of Nitroalkane Oxidase from Fusarium oxysporum Identifies a New Member of the Acyl-CoA Dehydrogenase Superfamily, Proc. Nat. Acad. Sci. USA 99, 2702-2707 *These authors contributed equally to the study.


20. Gadda, G. (2003) Kinetic Mechanism of Choline Oxidase from Arthrobacter globiformis, Biochim. Biophys. Acta 1646(1-2), 112-118

21. Gadda, G., and McAllister-Wilkins, E.E. (2003) Cloning, Expression, and Purification of Choline Dehydrogenase from the Moderate Halophile Halomonas elongata, Appl. Environ. Microbiol. 69(4), 2126-2132

22. Gadda, G. (2003) pH and Deuterium Kinetic Isotope Effects Studies on the Oxidation of Choline to Betaine-Aldehyde Catalyzed by Choline Oxidase, Biochim. Biophys. Acta 1650(1-2), 4-9

23. Ghanem, M., Fan, F., Francis, K., and Gadda, G. (2003) Spectroscopic and Kinetic Properties of Recombinant Choline Oxidase from Arthrobacter globiformis, Biochemistry 42, 15179-15188

24. Fan, F., Ghanem, M., and Gadda, G. (2004) Cloning, Sequence Analysis, and Purification of Choline Oxidase from Arthrobacter globiformis: a Bacterial Enzyme Involved in Osmotic Stress Tolerance, Arch. Biochem. Biophys. 421, 149-158

25. Gadda, G., Powell, N.L.N., and Menon, P. (2004) The Trimethylammonium Headgroup of Choline is a Major Determinant for Substrate Binding and Specificity in Choline Oxidase, Arch. Biochem. Biophys., 430, 264-273

26. Fan, F., and Gadda, G. (2005) On the Catalytic Mechanism of Choline Oxidase, J. Am. Chem. Soc. U.S.A., 127(7), 2067-2074

27. Ghanem, M., and Gadda, G. (2005) On the Catalytic Role of the Conserved Active Site His466 of Choline Oxidase, Biochemistry, 44, 893-904

28. Francis, K., Russell, B., and Gadda, G. (2005) Involvement of a Flavosemiquinone in the Enzymatic Oxidation of Nitroalkanes Catalyzed by 2-Nitropropane Dioxygenase, J. Biol. Chem., 280, 5195-5204

29. Fan, F., and Gadda, G. (2005) Oxygen- and Temperature-Dependent Kinetic Isotope Effects in Choline Oxidase: Correlating Reversible Hydride Transfer with Environmentally Enhanced Tunneling, J. Am. Chem. Soc. U.S.A., 127, 17954-17961

30. Fan, F., Germann, M.W., and Gadda, G. (2006) Mechanistic Studies of Choline Oxidase with Betaine Aldehyde and its Isosteric Analog 3,3-Dimethylbutyraldehyde, Biochemistry, 45, 1979-1986

31. Ghanem, M., and Gadda, G. (2006) Effects of Reversing the Protein Positive Charge in Proximity of the N(1) Flavin Locus of Choline Oxidase, Biochemistry, 45, 3437-3447

32. Gadda, G., Fan, F., and Hoang, J.V. (2006) On the Contribution of the Positively Charged Headgroup of Choline to Substrate Binding and Catalysis in the Reaction Catalyzed by Choline Oxidase, Arch. Biochem. Biophys., 451,182-187

33. Francis, K, and Gadda, G. (2006) Probing the Chemical Steps of Nitroalkane Oxidation Catalyzed by 2-Nitropropane Dioxygenase with Solvent Viscosity, pH, and Substrate Kinetic Isotope Effects, Biochemistry, 45, 13889-13898


34. Hoang, J.V., and Gadda, G. (2007) Trapping Choline Oxidase in a Nonfunctional Conformation by Freezing at Low pH, Proteins 66, 611-620

35. Zargari, A., Selander, C., Rasool, O., Ghanem, M., Gadda, G., Crameri, R., and Scheynius, A. (2007) Mala s 12 is a Major Allergen in Patients with Atopic Eczema and has Sequence Similarities to the GMC Oxidoreductase Family, Allergy 62, 695-703

36. Fan, F., and Gadda, G. (2007) An Internal Equilibrium Preorganizes the Enzyme-Substrate Complex for Hydride Tunneling in Choline Oxidase, Biochemistry 46, 6402-6408

37. Zou, J., Hofer, A.M., Lurtz, M.M., Gadda, G., Ellis, A.L., Chen, N., Huang, Y., Holder, A., Louis, C., Welshhans, K., Rehder, V., and Yang, J.J. (2007) Developing Sensors for real Time Measurement of High Ca++ Concentrations, Biochemistry 46, 12275-12288

38. Quaye, O., Lountos, G.T., Fan, F., Orville, A.M., and Gadda, G. (2008) Role of Glu312 in Binding and Positioning of the Substrate for Hydride Transfer Reaction in Choline Oxidase, Biochemistry 47, 243-256

39. Pennati, A., Aliverti, A., Zanetti, G., and Gadda, G. (2008) Effect of Salt and pH on the Reductive Half-Reaction of Mycobacterium tuberculosis FprA with NADPH, Biochemistry 47, 3418-3425

40. Mijatović, S., and Gadda, G. (2008) Oxidation of Alkyl Nitronates Catalyzed by 2-Nitropropane Dioxygenase from Hansenula mrakii, Arch. Biochem. Biophys. 473, 61-68

41. Rungsrisuriyachai K., and Gadda, G. (2008) On the Role of Histidine 351 in the Reaction of Alcohol Oxidation Catalyzed by Choline Oxidase, Biochemistry 47, 6762-6769

42. Francis, K., and Gadda, G. (2008) The Non-Oxidative Conversion of Nitroethane to Ethylnitronate in Neurospora crassa 2-Nitropropane Dioxygenase is Catalyzed by Histidine 196, Biochemistry 47, 9136-9144

43. Finnegan, S., and Gadda, G. (2008) Substitution of an Active Site Valine Uncovers a Kinetically Slow Equilibrium between Competent and Incompetent Forms of Choline Oxidase, Biochemistry 47, 13850-13861

44. Gadda, G. (2008) Hydride Transfer Made Easy in the Reaction of Alcohol Oxidation Catalyzed by Flavin-dependent Oxidases, Biochemistry 47, 13745-13753

45. Kamio, M., Ko, C., Zheng, S., Wang, B., Collins, S.L., Gadda, G., Tai, P.C., and Derby, C.D. (2009) The Chemistry of Escapin: Identification and Quantification of the Components in the Complex Mixture Generated by an L-Amino Acid Oxidase in the Defensive Secretion of the Sea Snail Aplysia californica, Chemistry 15, 1597-1603

46. Rungsrisuriyachai, K., and Gadda, G. (2009) A pH Switch Affects the Steady-State Kinetic Mechanism of Pyranose 2-Oxidase from Trametes ochracea, Arch. Biochem. Biophys. 483, 10-15

47. Orville, A.M., Lountous, G.T., Finnegan, S., Gadda, G., and Prabhakar, R. (2009) Crystallographic, Spectroscopic, and Computational Analysis of a Flavin-C4a-Oxygen Adduct in Choline Oxidase, Biochemistry 48, 720-728


48. Francis, K., and Gadda, G. (2009) Inflated Kinetic Isotope Effects in the Branched Mechanism of Neurospora crassa 2-Nitropropane Dioxygenase, Biochemistry 48, 2403-2410

49. Chen, N., Zou, J., Wang, S., Ye, Y., Huang, Y., Gadda, G., and Yang, J. (2009) Designing Protease Sensors for Real-time Imaging of Trypsin Activation in Pancreatic Cancer Cells, Biochemistry 48, 3519-3526

50. Quaye, O., Cowins, S., Gadda, G. (2009) Contribution of Flavin Covalent Linkage with Histidine 99 to the Reaction Catalyzed by Choline Oxidase, J. Biol. Chem. 284, 16990-16997

51. Francis, K., and Gadda, G. (2009) Kinetic Evidence for an Anion Binding Pocket in the Active Site of Nitronate Monooxygenase, Bioorg. Chem. 37, 167-172

52. Quaye, O., and Gadda, G. (2009) Effect of a Conservative Mutation of an Active Site Residue Involved in Substrate Binding on the Hydride Tunneling Reaction Catalyzed by Choline Oxidase, Arch. Biochem. Biophys. 489, 10-14

53. Xin, Y., Gadda, G., and Hamelberg, D. (2009) Cluster of Hydrophobic Residues Controls the Entrance to the Active Site of Choline Oxidase, Biochemistry 48, 9599-9605

54. Pennati, A., and Gadda, G. (2009) Involvement of Ionizable Groups in Catalysis of Human Liver Glycolate Oxidase, J. Biol. Chem. 284, 31214-31222

55. Gadda, G., and Francis, K. (2010) Nitronate Monooxygenase, a Model for Anionic Flavin Semiquinone Intermediates in Oxidative Catalysis, Arch. Biochem. Biophys. 493, 53-61

56. Rungsrisuriyachai, K., and Gadda, G. (2010) Role of Asn510 in the Relative Timing of Substrate Bond Cleavages in the Reaction Catalyzed by Choline Oxidase, Biochemistry 49, 2483-2490

57. Finnegan, S., Agniswamy, J., Weber, I., and Gadda, G. (2010) Role of Val464 in the Flavin Oxidation Reaction Catalyzed by Choline Oxidase, Biochemistry 49, 2952-2961

58. Quaye, O., Nguyen, T., Gannavaram, S., Pennati, A., and Gadda, G. (2010) Rescuing of the Hydride Transfer Reaction in the Glu312Asp Variant of Choline Oxidase by a Substrate Analogue, Arch. Biochem. Biophys. 499, 1-5

59. Finnegan, S., Yuan, H., Wang, Y.F., Orville, A.M., Weber, I., and Gadda, G. (2010) Structural and Kinetic Studies on the Ser101Ala Variant of Choline Oxidase: Catalysis by Compromise, Arch. Biochem. Biophys. 510, 207-213

60. Fu, G., Yuan, H., Li, C., Lu, C., Gadda, G., and Weber, I. (2010) Conformational Changes and Substrate Recognition in Pseudomonas aeruginosa D-Arginine Dehydrogenase, Biochemistry 49, 8538-8545

61. Yuan, H., Fu, G., Brooks, P., Weber, I., and Gadda, G. (2010) Steady State Kinetic Mechanism and Reductive Half-Reaction of D-Arginine Dehydrogenase from Pseudomonas aeruginosa, Biochemistry 49, 9542-9550

62. Pennati, A., and Gadda, G. (2011) Stabilization of an Intermediate in the Oxidative Half Reaction of Human Liver Glycolate Oxidase, Biochemistry 50, 1-3


63. Yuan, H., and Gadda, G. (2011) Importance of a Serine Proximal to the C(4a)-N(5) Flavin Atoms for Hydride Transfer in Choline Oxidase, Biochemistry 50, 770-779

64. Fu, G., Yuan, H., Wang, S., Gadda, G., and Weber, I. (2011) Atomic Resolution Structure of an N(5) Flavin Adduct in D-Arginine Dehydrogenase, Biochemistry 50, 6292-6294

65. Tang, S., Wong, H.C., Wang, Z.M., Hyuang, Y., Zou, J., Zhuo, Y., Pennati, A., Gadda, G., Delbono, O., and Yang, J.J. (2011) Design and Application of a Class of Sensors to Monitor Ca2+ Dynamics in High Ca2+ Concentration Cellular Compartments, Proc. Nat. Acad. Sci. U.S.A. 108, 16265-16270

66. Yuan, H., Xin, Y., Hamelberg, D., and Gadda, G. (2011) Insights on the Mechanism of Amine Oxidation Catalyzed by D-Arginine Dehydrogenase Through pH and Kinetic Isotope Effects, J. Am. Chem. Soc. U.S.A. 133, 18957-18965

67. Francis, K., Nishino, S.F., Spain, J.C., and Gadda, G. (2012) A Novel Activity for Fungal Nitronate Monooxygenase: Detoxification of the Metabolic Inhibitor Propionate-3-Nitronate, Arch. Biochem. Biophys. 521, 84-89

68. Gadda, G. (2012) Oxygen Activation in Flavoprotein Oxidases: the Importance of Being Positive, Biochemistry 51, 2662-2669

69. Gannavaram, S., and Gadda, G. (2013) Relative Timing of Hydrogen and Proton Transfers in the Reaction of Flavin Oxidation Catalyzed by Choline Oxidase, Biochemistry 52, 1221-1226

70. Smitherman, C., and Gadda, G. (2013) Evidence for a Transient Peroxynitro Acid in the Reaction Catalyzed by Nitronate Monooxygenase with Propionate 3-Nitronate, Biochemistry 52, 2694-2704

71. Lambou, K., Pennati, A., Valsecchi, I., Tada, R., Sherman, S., Sato, H., Beau, R., Gadda, G., and Latgé, J.P. (2013) Pathway of Glycine Betaine Biosynthesis in Aspergillus fumigatus, Eukaryot. Cell 12, 853-863

72. Gadda, G., and Fitzpatrick, P.F. (2013) Solvent Isotope and Viscosity Effects on Steady-State Kinetics of the Flavoprotein Nitroalkane Oxidase, FEBS Lett. 587, 2785-2789

73. Francis, K., Smitherman, C., Nishino, S.F., Spain, J.C., and Gadda, G. (2013) The Biochemistry of the Metabolic Poison Propionate 3-Nitronate and its Conjugate Acid, 3-Nitropropionate, IUBMB Life 65, 759-768

74. Salvi, F., and Gadda, G. (2013) Human Choline Dehydrogenase: Medical Promises and Biochemical Challenges, Arch. Biochem. Biophys. 537, 243-252

75. Ouattara, M., Pennati, A., Delvin, D.J., Huang, Y.S., Gadda, G., and Eichenbaum, Z., (2013) Kinetics of Heme Transfer by the Shr NEAT Domains of Group A Streptococcus, Arch. Biochem. Biophys. 538, 71-79

76. Anton, B.P., Chang, Y.C., Brown, P., Choi, H.P., Faller, L.L., Guleria, J., Hu, Z., Klitgord, N., Levy-Moonshine, A., Maksad, A., Mazumdar, V., McGettrick, M., Osmani, L., Pokrzywa, R., Rachlin, J., Swaminathan, R., Allen, B., Housman, G., Monahan, C., Rochussen, K., Tao, K., Bhagwat, A.S., Brenner, S.E., Columbus, L., de Crécy-Lagard, V., Ferguson, D.,


Fomenkov, A., Gadda, G., Morgan, R.D., Osterman, A.L., Rodionov, D.A., Rodionova, I.A., Rudd, K.E., Söll, D., Spain, J., Xu, S.Y., Bateman, A., Blumenthal, R.M., Bollinger, J.M., Chang, W.S., Ferrer, M., Friedberg, I., Galperin, M.Y., Gobeill, J., Haft, D., Hunt, J., Karp, P., Klimke, W., Krebs, C., Macelis, D., Madupu, R., Martin, M.J., Miller, J.H., O'Donovan, C., Palsson, B., Ruch, P., Setterdahl, A., Sutton, G., Tate, J., Yakunin, A., Tchigvintsev, D., Plata, G., Hu, J., Greiner, R., Horn, D., Sjölander, K., Salzberg, S.L., Vitkup, D., Letovsky, S., Segrè, D., DeLisi, C., Roberts, R.J., Steffen, M., Kasif, S. (2013) The COMBREX Project: Design, Methodology, and Initial Results, PLosS Biol. 11, e1001638

77. Salvi, F., Wang, Y.-F., Weber, I.T., and Gadda, G. (2014) Crystal Structure of Choline Oxidase in Complex with the Reaction Product Glycine Betaine, Acta Cryst. D 70, 405-413

78. Salvi, F., Agniswamy J., Yuan, H., Vercammen, K., Pelican, R., Cornelis, P., Spain, J., Weber, I.T., and Gadda, G. (2014) The Combined Structural and Kinetic Characterization of a Bacterial Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1 Establishes NMO Class I and II, J. Biol. Chem. 289, 23764-23775

79. Romero, E., and Gadda, G. (2014) Alcohol Oxidation by Flavoenzymes, Biomolecular Concepts 5, 299-318

80. Gannavaram, S., Sirin, S., Sherman, W., and Gadda, G. (2014) Mechanistic and Computational Studies on the Reductive Half-Reaction of Tyrosine to Phenylalanine Active Site Variants of D-Arginine Dehydrogenase, Biochemistry 53, 6574-6583.

81. Vercammen, K., Wei, Q., Charlier, D., Dötsch, A., Haüssler, S., Schulz, S., Salvi, F., Gadda, G., Spain, J., Levin Rybtke, M., Tolker-Nielsen, T., Dingemans, J., Ye, L., and Cornelis, P. (2014) Pseudomonas aeruginosa LysR PA4203 Regulator NmoR Acts as a Represson of the PA4202 nmoA Gene Encoding Nitronate Monooxygenase, J. Bacteriol., 197, 1026-1039.

82. Smitherman, C., Rungsrisuriyachai, K., Germann, M., and Gadda, G. (2015) Identification of the Catalytic Base for Alcohol Oxidation in Choline Oxidase, Biochemistry, 54, 413-421.

83. Ball, J., Bui, Q.V.V., Gannavaram, S., and Gadda, G. (2015) Importance of Glutamate 87 and the Substrate alpha-Amine for the Reaction Catalyzed by D-Arginine Dehydrogenase, Arch. Biochem. Biophys., 568, 56-63.

84. Barman, A., Smitherman, C., Souffrant, M., Gadda, G., and Hamelberg, D. (2016) Conserved Hydration Sites in Pin1 Reveal a Distinctive Water Recognition Motif in Proteins, J. Chem. Info. Model. 56, 139-147.

85. Romero, E., Tork Ladani, S., Hamelberg, D., and Gadda, G. (2016) Solvent-Slaved Motions in the Hydride Tunneling Reaction Catalyzed by Human Glycolate Oxidase, ACS Catalysis 6, 2113-2120.

86. Salvi, F., Rodriguez, I., Hamelberg, D., and Gadda, G. (2016) Role of F357 as Oxygen Gate in the Oxidative Half-reaction of Choline Oxidase, Biochemistry 55, 1473-1484.

87. Sachla, A.J., Ouattara, M., Romero, E., Agniswamy, J., Weber, I.T., Gadda, G., and Eichenbaum, Z. (2016) In Vitro Heme Biotransformation by the HupZ Enzyme from Group A Streptococcus, Biometals 29, 593-609.


88. Ball, J., Salvi, F., and Gadda, G. (2016) Functional Annotation of a Presumed Nitronate Monooxygenase Reveals a New Class of NADH:quinone Reductases, J. Biol. Chem. 291, 21160-21170.

c.vi) Book/Encyclopedia Chapters/Editorials (total: 5)

1. Edmondson, D.E. and Gadda, G. (2008) Guidelines for the Functional Analysis of Engineered and Mutant Enzymes; in Protein Engineering Handbook; S. Lutz and U.T. Bornscheuer Eds.; Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany

2. Gadda, G. (2010) Special Issue on Oxidative Enzymes, Guest Editor’s Introduction, Arch. Biochem. Biophys., 493, 1-2

3. Gadda, G. (2012) Flavins; in Encyclopedia of Biophysics; G.C. Roberts, Ed.; Springer-Verlag GmbH, Berlin, Germany

4. Gadda, G. (2013) Choline Oxidase and Related Systems; in Handbook of Flavoproteins; S.M. Miller, Bruce A. Palfey, R. Hille, Eds.; Walter de Gruyter GmbH & Co., KG, Berlin, Germany, 155-176

5. Gadda G. (2014) Cofactor Assisted Enzymatic Catalysis, Guest Editor’s Introduction, Arch. Biochem. Biophys., 544, 1

c.vii) Book Reviews (total: 1)

1. Gadda, G. (2007) Flavins: Photochemistry and Photobiology. Comprehensive Series in Photochemical and Photobiological Sciences Edited by Eduardo Silva and Ana M. Edwards (P. Universidad Catolica de Chile, Santiago). Royal Society of Chemistry: Cambridge. 2006. x + 328 pp. $329.00. ISBN 0-85404-331-4. J. Am. Chem. Soc. 2007 Jul 18;129(28):8926.

c.viii) Contributed Papers to Conference Proceedings (total: 14)

1. Gadda, G., Aliverti, A., Ronchi, S. and Zanetti, G. (1991) Ferredoxin Binding Site of Ferredoxin-NADP+ Reductase as Explored by Limited Proteolysis and Mutagenesis, in Flavins and Flavoproteins (Curti, B., Ronchi, S. and Zanetti, G., eds.) pp. 465-468, Walter de Gruyter, Berlin

2. Gadda, G., Negri, A. and Pilone, M.S. (1994) Chemical Modification of Arginine Groups in D-Amino Acid Oxidase from Rhodotorula gracilis, in Flavins and Flavoproteins (Yagi, K., ed.) pp. 167-170, Walter de Gruyter, Berlin

3. Faotto, L., Pollegioni, L., Ceciliani, F., Gadda, G., Ronchi, S. and Pilone, M.S. (1994) Amino Acid Sequence of D-Amino Acid Oxidase from the Yeast Rhodotorula gracilis, in Flavins and Flavoproteins (Yagi, K., ed.) pp. 163-166, Walter de Gruyter, Berlin

4. Gadda, G., and Fitzpatrick, P.F. (1997) Characterization of the Flavin Adduct of Nitroalkane Oxidase, in Flavins and Flavoproteins (Stevenson, K.J., Massey, V. and Williams, C.H.Jr., eds.) pp. 147-150, University of Calgary Press, Calgary


5. Fitzpatrick, P.F., Kurtz, K., Gadda, G., Rishavy, M., and Cleland, W.W. (1999) Mechanisms of Flavoprotein Oxidases, in Proceedings of the 26th Steenbock Symposium (Frey, P.A., and Northrop, D.B., eds.) pp. 176-186, IOS Press, Amsterdam

6. Gadda, G., and Fitzpatrick, P.F. (2000) An Iso-Mechanism for Nitroalkane Oxidase: Evidence for a Slow Proton Transfer to Solvent Coupled to Isomerization of the Free reduced Enzyme, in Flavins and Flavoproteins, (Ghisla, S., Kroneck, P., Sund, H., and Macheroux, P., eds.) pp. 639-642, Rudolf Weber, Berlin, Germany

7. Gadda, G. (2002) Mechanistic Studies on Choline Oxidase From Arthrobacter globiformis, in Proceedings of the Fourteenth International Symposium on Flavins and Flavoproteins, (Perham, R.N., Chapman, S.K., Scrutton, N.S., eds.) p. 181-186, Rudolf Weber, Berlin, Germany

8. Powell, N., Fan, F., Wilkins, L., and Gadda, G. (2002) Comparative Study of Choline Oxidizing Enzymes From Escherichia coli, Halomonas elongata, and Arthrobacter globoiformis, in Proceedings of the Fourteenth International Symposium on Flavins and Flavoproteins, (Perham, R.N., Chapman, S.K., Scrutton, N.S., eds.) p. 149-154, Rudolf Weber, Berlin, Germany

9. Russell, B., Johnson, K.L., Seyfe, D., and Gadda, G. (2002) Purification and Characterization of Recombinant 2-Nitropropane Dioxygenase From Neurospora crassa and Hansenula mrakii, in Proceedings of the Fourteenth International Symposium on Flavins and Flavoproteins, (Perham, R.N., Chapman, S.K., Scrutton, N.S., eds.) p. 229-234, Rudolf Weber, Berlin, Germany

10. Daubner, S.C., Gadda, G, Valley, M.P., and Fitzpatrick, P.F. (2002) Nitroalkane Oxidase from Fusarium oxysporum is a Member of the Acyl-CoA Dehydrogenase Superfamily, in Proceedings of the Fourteenth International Symposium on Flavins and Flavoproteins, (Perham, R.N., Chapman, S.K., Scrutton, N.S., eds.) p. 981-986, Rudolf Weber, Berlin, Germany

11. Fitzpatrick, P.F., Valley, M.P., Gadda, G., Nagpal, A., and Orville, A.M. (2005) The Mechanism of Nitroalkane Oxidase, in Proceedings of the Fifteen International Symposium on Flavins and Flavoproteins, (Nishino, T., Miura, R., Tonukora, M., and Fukui, K., eds.) p. 59-69, Archi Tect inc, Tokyo, Japan

12. Gadda, G., Pennati, A., Francis, K., Quaye, O., Yuan, H., Rungsrisuriyachai, K., Finnegan, S., Mijatovic, S., Nguyen, T., and Orville, A.M. (2008) Hydride Transfer Made Easy in the Oxidation of Alcohols Catalyzed by Choline Oxidase, in Proceedings of the Sixteenth International Symposium on Flavins and Flavoproteins, (S. Frago, Gomez-Moreno, C. and Medina M., eds.) p. 309-314, Prensas Universitarias de Zaragoza

13. Smitherman, C., Francis, K., Nishino, S., Spain, J., and Gadda, G. (2011) Detoxification of a Lethal Toxin by Nitronate Monooxygenase, in Proceedings of the Seventeenth International Symposium on Flavins and Flavoproteins, (Miller, S., Hille, R., and Palfey, B., eds.) p. 365-370


14. Gadda, G. (2011) Oxygen Activation in Choline Oxidase: the Value of Positive Substrates, in Proceedings of the Seventeenth International Symposium on Flavins and Flavoproteins, (Miller, S., Hille, R., and Palfey, B., eds.) p. 99-110

c.ix) Invited Seminars (total: 48)

2017 July 5th - TBA, 19th International Symposium on Flavins and Flavoproteins, Gröningen, The Netherlands

2017 February 3rd - “Isotope Effects as Probes for the Mechanism of Action of Flavoproteins”, Georgia State University Biology, Atlanta, GA, U.S.A.

2015 May 8th - “Structural and Kinetic Characterization of Bacterial Nitronate Monooxygenase Establish NMO Class I & II” FAME 2015 – The Florida Annual Meeting and Exposition, Tampa FL, U.S.A.

2014 April 17th - “Biochemical and Structural Studies to Decipher how Flavin Versatility is Tamed in Flavoenzymes” Georgia Perimeter College, Decatur GA, U.S.A.

2013 July 17th - “How a Single Electron and a Flavin Save Lives: the Case of Nitronate Monooxygenase” Gordon Research Conference of Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley Resort, Waterville Valley, NH, U.S.A.

2013 March 29th - “Detoxification of the Metabolic Poison Propionate-3-Nitronate by Nitronate Monooxygenase”, University of Miami, Miami FL, U.S.A.

2013 February 22nd - “A Novel Activity for Fungal Nitronate Monooxygenase: Detoxification of the Metabolic Inhibitor Propionate-3-nitronate”, Georgia State University, Atlanta GA, U.S.A.

2012 May 18th - “Detoxification of the Metabolic Poison Propionate-3-Nitronate by Nitronate Monooxygenase” FAME 2012 – The Florida Annual Meeting and Exposition, Tampa FL, U.S.A.

2012 April 20th - “A Novel Activity for Fungal Nitronate Monooxygenase: Detoxification of the Metabolic Poison Propionate-3-Nitronate”, University of Kentucky, Lexington KY, U.S.A.

2012 April 3rd - “Choline Oxidase and Related Systems”, University of Nebraska, Lincoln NE, U.S.A.

2012 March 30th - “The Biochemistry and Biophysics of Choline Oxidase”, University of Florida, Gainesville FL, U.S.A.

2012 January 27th - “Oxygen Activation in Flavoprotein Oxidases: the Importance of Positive Substrates in Choline Oxidase”, SUNY Buffalo, Buffalo NY, U.S.A.

2011 October 20th - “Biochemical Studies of Flavin-Dependent Enzymes and Other Chemical Projects at GSU” College of Charleston, Charleston SC, U.S.A.

2011 October 19th - “Mechanistic Enzymology and Other Research Opportunities at GSU”, Kennesaw State University, Kennesaw GA, U.S.A.


2011 July 25th - “Oxygen Activation in Choline Oxidase: the Value of Positive Substrates”, 17th International Symposium on Flavins and Flavoproteins, Berkeley CA, U.S.A.

2011 May 13th - “Mechanistic Investigation of D-Arginine Dehydrogenase from Pseudomonas aeruginosa”, FAME 2011 – The Florida Annual Meeting and Exposition, Tampa FL, U.S.A.

2011 April 18th - “Mechanistic Studies on Human Liver Glycolate Oxidase”, Virginia Polytechnic Institute and State University, Blacksburg VI, U.S.A.

2010 October 6th - “Structural-Functional Studies on Nitronate Monooxygenase”, University of Tennessee, Knoxville TN, U.S.A.

2010 September 9th - “Investigations of Flavoprotein Oxidases”, University of Alabama, Tuscaloosa AL, U.S.A.

2009 October 23rd – “Mechanistic investigations of flavoprotein oxidases”, Western Carolina University, Cullowhee NC, U.S.A.

2009 July 10th – “Branching of a reaction intermediate formed during turnover of N. crassa 2-nitropropane dioxygenase inflates the observed kinetic isotope effect for the reaction”, Gordon Research Conference of Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley Resort, Waterville Valley NH, U.S.A.

2009 May 15th – “Involvement of Ionizable Groups in the Reaction Catalyzed by Human Liver Glycolate Oxidase”, FAME 2009 – The Florida Annual Meeting and Exposition, Orlando FL, U.S.A.

2009 February 5th – “Effect of protein flavinylation on the reaction of hydride ion transfer catalyzed by flavoenzymes” Auburn University, Auburn AL, U.S.A.

2008 October 28th – “Enzyme-substrate preorganization in the Alcohol Oxidation Catalyzed by Choline Oxidase”, The 2nd International Interdisciplinary Conference on Vitamins, Coenzymes, and Biofactors, The University of Georgia, Athens GA, U.S.A.

2008 September 11th – “Does Flavin Covalent Linkage to the Protein Affect Preorganization of the Enzyme-Substrate complex in Choline Oxidase?” University of South Florida, Tampa FL, U.S.A.

2008 May 9th – “On the Importance of Enzyme-Substrate Preorganization in the Hydride Ion Tunneling Reaction Catalyzed by Choline Oxidase”, FAME 2008 – The Florida Annual Meeting and Exposition, Orlando FL, U.S.A.

2008 February 18th – “Partition Isotope Effects for Enzymatic Reactions Occurring through Multiple Pathways: The Observed Kinetic Isotope Effect in the 2-Nitropropane Dioxygenase Reaction”, Gordon Research Conference on Isotopes in Biological and Chemical sciences, Ventura CA, U.S.A.


2007 December 20th - “2-Nitropropane Dioxygenase: a Model for Anionic Semiquinone Intermediates in Flavin-Dependent Oxidation Reactions”, Universita' degli Studi di Milano, Milano, Italy

2007 November 2nd - “Mechanistic Studies of Flavoprotein Oxidases”, Tuskegee University, Tuskegee AL, U.S.A.

2007 September 25th - "Involvement of a Flavosemiquinone in the Oxidation Reaction Catalyzed by 2-Nitropropane Dioxygenase", University of Michigan, Ann Arbor MI, U.S.A.

2007 August 22th - "Flavin-Dependent Oxidation of Alcohols", Symposium entitled: “Strategies in Enzymatic Oxidation Catalysis”, at the 234th National Meeting of the American Chemical Society, Boston MA, U.S.A.

2007 July 20th - "Hydride transfer made easy in the oxidation of alcohols catalyzed by flavin-dependent enzymes", Georgia State University, Atlanta GA, U.S.A.

2006 December 18th - "The Unusual Mechanism of 2-Nitropropane Dioxygenase: a Model for Anionic Flavosemiquinone Intermediates in Enzymatic Catalysis", Universita' dell' Insubria, Varese, Italy

2006 October 6th - "Alcohol Oxidation by Flavoprotein Oxidases: the Chemical Mechanism of Choline Oxidase", Flavins and Flavoproteins in Milano, a Symposium to Honor the Career of Bruno Curti, Universita' degli Studi di Milano, Milano, Italy

2006 September 15th - "Alcohol Oxidation by Flavoprotein Oxidases: the Chemical Mechanism of Choline Oxidase", Georgia State University, Atlanta GA, U.S.A.

2006 July 19th - "Hydride Transfer Made Easy: the Chemical Mechanism for Alcohol Oxidation in Choline Oxidase", Gordon Research Conference of Enzymes, Coenzymes, and Metabolic Pathways, University of New England, Biddeford ME, U.S.A.

2004 March 31th - “Structure-Function Studies of FAD-Containing Choline Oxidase: a Bacterial Enzyme Involved in Stress Response", Department of Chemistry, Clark Atlanta University, Atlanta GA, U.S.A.

2004 March 12th - “Mechanistic and Biochemical Investigation of Choline-Oxidizing Enzymes Involved in Bacterial Stress Response", Department of Genetics and Microbiology, Universitita' degli Studi di Milano, Milano, Italy

2004 March 11th - “Biochemical and Mechanistic Investigation of Choline-Oxidizing Enzymes Involved in Bacterial Stress Response", Department of Genetics and Microbiology, Universitita' degli Studi di Pavia, Pavia, Italy

2003 December 5th - “Choline Oxidase and Bacterial Stress Tolerance: Mechanistic and Biochemical Investigation”, Department of Chemistry, University of Wisconsin at Milwaukee, Milwaukee WI, U.S.A.


2003 April 3rd - “Mechanistic Studies on Choline Oxidase: a Bacterial Enzyme Involved in Stress Response”, Department of Chemistry, University of Georgia, Athens GA, U.S.A.

2002 May 10th – “Nitroalkane-Oxidizing Flavin-Dependent Enzymes”, Shearwater Corporation, Huntsville AL, U.S.A.

2001 December 21th - “Mechanistic studies on flavoprotein oxidoreductases”, Department of General Physiology and Biochemistry, Universita’ degli Studi di Milano, Milano, Italy

2001 September 24th - “Biochemical studies on flavin-dependent enzymes: towards a better understanding of flavin reactivity modulation”, 53rd Southeast Regional Meeting of the American Chemical Society, Protein Engineering Session, Savannah GA, U.S.A.

2000 13th April - “Structural and mechanistic characterization of a nitroalkane-oxidizing enzyme from Fusarium oxysporum”, Department of Biochemistry and Microbiology, Rutgers University, New Brunswick NJ, U.S.A.

2000 28th February - “Structural and mechanistic characterization of a nitroalkane-oxidizing enzyme from Fusarium oxysporum”, Departments of Biology and Chemistry, Georgia State University, Atlanta GA, U.S.A.

1997 “Identification of the naturally occurring flavin of nitroalkane oxidase from Fusarium oxysporum”, Department of Chemistry, Texas A & M University, College Station TX, U.S.A.

1995 “Characterization of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum”, First European Network Meeting FLAPS 1, University of Wageningen, Wageningen, The Netherlands

1990 “Chemical modification and site-directed mutagenesis of lysine 116 of ferredoxin-NADP+ reductase”, Fifth National Congress Proteine ’90, Urbino, Italy

c.x) Conference Abstracts (total: 195)

1. Zanetti, G., Gadda, G., Aliverti, A. and Ronchi, S. (1989) Limited proteolysis of ferredoxin-NADP+ oxidoreductase, Proceedings of the 19th FEBS Meeting, Rome, Italy

2. Gadda, G., Aliverti, A., Ronchi, S. and Zanetti, G. (1990) Ferredoxin-NADP+ reductase as explored by limited proteolysis and mutagenesis, Proceedings of the Tenth International Symposium on Flavins snd Flavoproteins, Como, Italy

3. Aliverti, A., Gadda, G., Ronchi, S. and Zanetti, G. (1990) Chemical modification and site-directed mutagenesis of Lysine 116 of ferredoxin-NADP+ reductase, Proceedings of the V National Congress Proteine '90, Urbino, Italy

4. Buto' S., Pollegioni L., Gadda, G., and Pilone M.S. (1992) Immobilized yeast D-amino acid oxidase, preparation and kinetic studies, Proceedings of the VII National Congress Proteine '92, Pavia, Italy


5. Gadda, G., Negri, A. and Pilone, M.S. (1993) Chemical modification of arginine groups in D-amino acid oxidase from Rhodotorula gracilis, Proceedings of the Eleventh International Symposium on Flavins and Flavoproteins, Nagoya, Japan

6. Faotto, L., Pollegioni, L., Ceciliani, F., Gadda, G., Ronchi, S. and Pilone, M.S. (1993) Amino acid sequence of D-amino acid oxidase from the yeast Rhodotorula gracilis, Proceedings of the Eleventh International Symposium on Flavins and Flavoproteins, Nagoya, Japan

7. Gadda, G., Negri, A. and Pilone M.S. (1993) Phenylglyoxal modification of Rhodotorula gracilis D-amino acid oxidase, Proceedings of the VIII National Congress Proteine '93, Parma, Italy

8. Gadda, G., Beretta, G. and Pilone, M.S. (1994) Studies on the active centre of the Rhodotorula gracilis D-amino acid oxidase, Proceedings of the IX National Congress Proteine '94, Verona, Italy

9. Gadda, G., Beretta, G.L. and Pilone, M. (1994) Chemical modification of D-amino acid oxidase from the yeast Rhodotorula gracilis with TNBS, Ital. J. Biochem.

10. Gadda, G. and Pilone, M. (1994) Active site topography in Rhodotorula gracilis D-amino acid oxidase, Proceedings of the XXXIX National Congress of the Italian Society of Biochemistry, Pavia, Italy

11. Pilone, M.S., Pollegioni, L., Gadda, G., Buto', S., Campaner, S., Beretta, G.L. and Molla, G. (1995) Structure-function relationship of D-amino acid oxidase from Rhodotorula gracilis, First European Symposium of the Protein Society, Davos, Switzerland

12. Gadda, G., Pilone, M.S., Ghisla, S. and Ambrosius, D. (1995) Characterization of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum, First European Symposium of the Protein Society, Davos, Switzerland

13. Gadda, G., Heasley, C.J. and Fitzpatrick, P.F. (1996) Characterization of the flavin adduct of nitroalkane oxidase, Proceedings of the Twelfth International Symposium on Flavins and Flavoproteins, Calgary, Canada

14. Gadda, G. and Fitzpatrick, P.F. (1997) Characterization of the Active FAD-Containing Form of Nitroalkane Oxidase, 17th International Congress of Biochemistry and Molecular Biology, San Francisco, U.S.A.

15. Gadda, G., and Fitzpatrick, P.F. (1998) Kinetic mechanism of nitroalkane oxidase from Fusarium oxysporum, 26th Steenbock Symposium “Enzymatic Mechanisms”, Madison, Wisconsin, U.S.A.

16. Gadda, G., and Fitzpatrick, P.F. (1999) An Iso-Mechanism for Nitroalkane Oxidase: Evidence for a Slow Proton Transfer to Solvent Coupled to Isomerization of the Free reduced Enzyme, Proceedings of the Thirteen International Symposium on Flavins and Flavoproteins, Konstanz, Germany


17. Gadda, G., Choe, D.Y., and Fitzpatrick, P.F. (2000) Substrate Hydrophobicity Dictates Rates of Binding in Nitroalkane Oxidase, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Ventura, California, U.S.A.

18. Verdel, E., Breyer, E., Gadda, G., and O'Hern, P. (2000) Are Lessons using Interactive Molecular Visualization Superior to Traditional Lessons in Biochemistry? Joint Southeast-Southwest Regional Meeting of the American Chemical Society, New Orleans, LA, U.S.A.

19. Gadda, G. (2001) pH and Deuterium Isotope Effects on the Reaction Catalyzed by the FAD-Containing Choline Oxidase, 17th Enzyme Mechanisms Conference, Marco Island, Florida, U.S.A.

20. Johnson, K.L., Kanazawa, J.T., McAllister, E., Russell, B., and Gadda, G. (2001) Structure-Function Studies of Flavin-Dependent Enzymes, 9th Annual F.L. (Bud) Suddath Memorial Symposium “Structural Biology”, Atlanta, Georgia, U.S.A.

21. Russell, B., and Gadda, G. (2001) Expression in Escherichia coli of Choline Dehydrogenase From Sinorhizobium meliloti, 53rd Southeast Regional Meeting of the American Chemical Society, Savannah, Georgia, U.S.A.

22. Russell, B., and Gadda, G. (2001) Expression in Escherichia coli of Choline Dehydrogenase From Sinorhizobium meliloti, Nations Symposium, Atlanta, Georgia, U.S.A.

23. Johnson, K.L., and Gadda, G. (2002) Purification and Characterization of FMN-Containing 2-Nitropropane Dioxygenase from Neurospora crassa, 10th Annual F.L. (Bud) Suddath Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, Georgia, U.S.A.

24. Fan F., and Gadda, G. (2002) Cloning of codA Gene Coding for Choline Oxidase from Arthrobacter globiformis, 10th Annual F.L. (Bud) Suddath Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, Georgia, U.S.A.

25. Seyfe, D., and Gadda, G. (2002) Expression, Purification, and Enzymatic Activity of FAD-Containing 2-Nitropropane Dioxygenase from Hansenula mrakii, 10th Annual F.L. (Bud) Suddath Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, Georgia, U.S.A.

26. Russell, B., and Gadda, G. (2002) Expression in Escherichia coli and Refolding of Choline Dehydrogenase from Sinorhizobium meliloti, 10th Annual F.L. (Bud) Suddath Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, Georgia, U.S.A.

27. Wilkins, L., and Gadda, G. (2002) Cloning, Expression, and Preliminary Studies of Choline Dehydrogenase from Halomonas elongata, 10th Annual F.L. (Bud) Suddath Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, Georgia, U.S.A.

28. Powell, N., and Gadda, G. (2002) Cloning, Expression, and Preliminary Characterization Studies of Choline Dehydrogenase from Escherichia coli, 10th Annual F.L. (Bud) Suddath


Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, Georgia, U.S.A.

29. Gadda, G. (2002) Mechanistic Studies on Choline Oxidase From Arthrobacter globiformis, Fourteenth International Symposium on Flavins and Flavoproteins, Cambridge, UK

30. Fan, F., Powell, N., Wilkins, L., and Gadda, G. (2002) Comparative Study of Choline Oxidizing Enzymes From Escherichia coli, Halomonas elongata, and Arthrobacter globiformis, Fourteenth International Symposium on Flavins and Flavoproteins, Cambridge, UK

31. Johnson, K.L., Russell, B., Seyfe, D., and Gadda, G. (2002) Purification and Characterization of Recombinant 2-Nitropropane Dioxygenase From Neurospora crassa and Hansenula mrakii, Fourteenth International Symposium on Flavins and Flavoproteins, Cambridge, UK

32. Daubner, S.C., Gadda, G, and Fitzpatrick, P.F. (2002) Nitroalkane Oxidase from Fusarium oxysporum is a Member of the Acyl-CoA Dehydrogenase Superfamily, Fourteenth International Symposium on Flavins and Flavoproteins, Cambridge

33. Russell, B., and Gadda, G. (2003) Steady State Kinetic Studies of Neurospora crassa 2-Nitropropane Dioxygenase, 18th Enzyme Mechanisms Conference, Galveston, Texas, U.S.A.

34. Francis K., and Gadda, G. (2003) Kinetic and Spectroscopic Studies on the Oxidized Form of Choline Oxidase, 11th Annual F.L. (Bud) Suddath Memorial Symposium “Cancer Research in Georgia”, Atlanta, Georgia, U.S.A.

35. Ghanem M., and Gadda, G. (2003) Biophysical and Kinetic Characterization of Choline Oxidase from Arthrobacter globiformis, 11th Annual F.L. (Bud) Suddath Memorial Symposium “Cancer Research in Georgia”, Atlanta, Georgia, U.S.A.

36. Powell, N., Ghanem, M., Francis, K., Fan F., and Gadda, G. (2003) Active Site Characterization of Recombinant Choline Oxidase, 29th Steenbock Symposium “Coenzymes, Cofactors and Catalysis, Madison”, Wisconsin, U.S.A.

37. Fan F., and Gadda, G. (2003) pH and Kinetic Isotope Effect Studies on Recombinant Choline Oxidase from Arthrobacter globiformis, 29th Steenbock Symposium “Coenzymes, Cofactors and Catalysis”, Madison, Wisconsin, U.S.A.

38. Menon, P., Fan, F., Ghanem, M., Francis, K., Powell, N., Nguyen, T., Tolbert, A., and Gadda, G. (2003) Biochemical and Kinetic Characterization of Choline Oxidase from Arthrobacter globiformis, 55rd Southeast Regional Meeting of the American Chemical Society, Atlanta, Georgia, U.S.A.

39. Fan, F., and Gadda, G. (2005) On the Mechanism of Alcohol Oxidation Catalyzed by Choline Oxidase, 19th Enzyme Mechanisms Conference, Asilomar, California, U.S.A.

40. Zheng, J., Ghanem, M., Gadda, G., and Shamshi, S.A. (2005) Simultaneous, Quantitative Analysis of Choline, Betaine Aldehyde, and Glycine Betaine Using Capillary Zone Electrophoresis-Electrospray Mass Spectrometry (CZE-ESI-MS): Kinetic Studies of


Choline Oxidases, Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Orlando, Florida, U.S.A.

41. Francis, K., and Gadda, G. (2006) The Catalytic Mechanism of 2-Nitropropane Dioxygenase Involves the Formation of an Anionic Semiquinone, 3rd Annual SER-CAT Symposium, Atlanta, GA, U.S.A.

42. Quaye, O. and Gadda, G. (2006) Mechanistic Investigation of the Glu312Asp Variant of Choline Oxidase, 3rd Annual SER-CAT Symposium, Atlanta, GA, U.S.A.

43. Rungsrisuriyachai, K., and Gadda, G. (2006) Investigation of the Active Site Histidine 351 of Choline Oxidase, 3rd Annual SER-CAT Symposium, Atlanta, GA, U.S.A.

44. Hoang, J., V., and Gadda, G. (2006) The Hysteretic Behavior of Choline Oxidase, 3rd Annual SER-CAT Symposium, Atlanta, GA, U.S.A.

45. Belaineh, M., and Gadda, G. (2006) Role of the Conserved Residue His196 of 2-Nitropropane Dioxygenase, 3rd Annual SER-CAT Symposium, Atlanta, GA, U.S.A.

46. Francis, K., and Gadda, G. (2006) An Anionic Flavosemiquinone is Involved in the Enzymatic Oxidation of Nitroalkanes Catalyzed by 2-Nitropropane Dioxygenase, 2nd Annual National Symposium on Prostate Cancer, Atlanta, GA, U.S.A.

47. Ghanem, M., and Gadda, G. (2006) Effects of the Protein Charge Close to the N(1)-Flavin Locus on Choline Oxidase, 2nd Annual National Symposium on Prostate Cancer, Atlanta, GA, U.S.A.

48. Hoang, J.V., and Gadda, G. (2006) Reversible Inactivation of Choline Oxidase by Freezing at Low pH, 2nd Annual National Symposium on Prostate Cancer, Atlanta, GA, U.S.A.

49. Francis, K., and Gadda, G. (2006) The Oxidation of Nitroalkanes by 2-Nitropropane Dioxygenase: Involvement of a Flavosemiquinone in Catalysis, 231st American Chemical Society National Meeting, Atlanta, GA, U.S.A.

50. Ghanem, M., and Gadda, G. (2006) On the Effects of Reversing the Protein Positive Charge in Proximity of the N(1)-Flavin Locus in Choline Oxidase, Experimental Biology 2006, San Francisco, California, U.S.A.

51. Chen, N., Zou, J., Gadda, G., Wang, S., and Yang, J.J. (2006) Developing Protease Sensors by Protein Design and Engineering, 14th Annual Suddath Symposium, Georgia Institute of Technology, Atlanta, Georgia, U.S.A.

52. Chen, N., Zou, J., Gadda, G., Wang, S., and Yang, J.J. (2006) Developing Protease Sensors by Protein Design and Engineering, Brain and Behavior Conference, Georgia State University, Atlanta, Georgia, U.S.A.

53. Zou, J., Ye, Y., Gadda, G., Ellis, A.L., Chen, N., and Yang, J.J. (2006) Development of Fluorescent Ca2+ Sensor for Cellular Imaging, 14th Annual Suddath Symposium, Georgia Institute of Technology, Atlanta, Georgia, U.S.A.


54. Gadda, G. (2006) Hydride Transfer Made Easy: the Chemical Mechanism for Alcohol Oxidation in Choline Oxidase, Gordon Research Conference of Enzymes, Coenzymes, and Metabolic Pathways, University of New England, Biddeford, ME, U.S.A.

55. Finnegan, S., Hoang, J.V., and Gadda, G. (2007) Importance of Val464 in Catalysis of Choline Oxidase, 20th Enzyme Mechanisms Conference, St. Pete Beach, Florida, U.S.A.

56. Francis, K., Belaineh, M., and Gadda, G. (2007) Reductive Half Reaction of 2-Nitropropane Dioxygenase: Involvement of an Electrostatic Catalyst in the Formation of an Anionic Flavosemiquinone, St. Pete Beach, Florida, U.S.A.

57. Mijatovic, S., Nguyen, T., and Gadda, G. (2007) Reevaluation of the Prosthetic Group and Substrate Specificity of 2-Nitropropane Dioxygenase from Hansenula mrakii, St. Pete Beach, Florida, U.S.A.

58. Quaye, O., and Gadda, G. (2007) On the Role of Glu312 in the Binding and Correct Positioning of the Substrate for Efficient Catalysis in Choline Oxidase, St. Pete Beach, Florida, U.S.A.

59. Rungsrisuriyachai, K., and Gadda, G. (2007) On the Role of Active Site Histidine 351 in the Reaction Catalyzed by Choline Oxidase, St. Pete Beach, Florida, U.S.A.

60. Quaye, O., and Gadda, G. (2007) Role of Glu312 in the Catalytic Mechanism of Choline Oxidase, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, Georgia, U.S.A.

61. Francis, K., and Gadda, G. (2007) Role of the Anionic Flavosemiquinone in the Reductive Half-Reaction of 2-Nitropropane Dioxygenase, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, Georgia, U.S.A.

62. Yuan, H., and Gadda, G. (2007) Kinetic Analysis of Ser101 Active Site Variants of Choline Oxidase, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, Georgia, U.S.A.

63. Rungsrisuriyachai, K., and Gadda, G. (2007) Role of His351 in the Active Site of Choline Oxidase, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, Georgia, U.S.A.

64. Mijatovic, S., and Gadda, G. (2007) Biophysical and Biochemical Characterization of 2-Nitropropane Dioxygenase from Hansenula mrakii, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, Georgia, U.S.A.

65. Nguyen, T., and Gadda, G. (2007) Rescuing the Activity of Choline Oxidase E312D Mutant Using a Substrate Analogue, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, Georgia, U.S.A.

66. Gadda, G., Francis, K., and Belaineh, M. (2007) The Mechanism of 2-Nitropropane Dioxygenase: a Model for Flavin Semiquinone Intermediates in Enzymatic Catalysis, Experimental Biology 2007, Washington, D.C., U.S.A.

67. Gadda, G., Rungsrisuriyachai, K., Quaye, O., Finnegan, S., Yuan, H., Nguyen, T., and Orville, A.M. (2007) Preorganization of the Enzyme-Substrate Complex in the Reaction of


Alcohol Oxidation Catalyzed by Choline Oxidase, Gordon Research Conference of Enzymes, Coenzymes, and Metabolic Pathways, University of New England, Biddeford, ME, U.S.A.

68. Francis, K., and Gadda, G. (2007) Probing the Nonoxidative Tautomerization of Nitroethane to Ethylnitronate Catalyzed by 2-NItropropane Dioxygenase with pH and Substrate Kinetic Isotope Effects. 234th ACS National Meeting, Boston, MA, U.S.A.

69. Francis, K., and Gadda, G. (2008) Partition Isotope Effects for Enzymatic Reactions Occurring Through Multiple Pathways: The Observed Kinetic Isotope Effect in the 2-Nitropropane Dioxygenase Reaction, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Ventura, California, U.S.A.

70. Orville, A.M., Prabhakar, R., Lountous, G.T., Finnegan, S., and Gadda, G. (2008) A Flavin-C4a-Oxygen Intermediate Observed by Crystallographic, Spectroscopic and Computational Methods, Sixteenth International Symposium on Flavins and Flavoproteins, Jaca, Spain

71. Gadda, G., Pennati, A., Francis, K., Quaye, O., Yuan, H., Rungsrisuriyachai, K., Finnegan, S., Mijatovic, S., Nguyen, T., and Orville, A.M. (2008) Hydride Transfer Made Easy in the Oxidation of Alcohols Catalyzed by Choline Oxidase, Sixteenth International Symposium on Flavins and Flavoproteins, Jaca, Spain

72. Finnegan, S., and Gadda, G. (2008) Hydrophobic Packing effects on the Reductive Half-reaction of Choline Oxidase, Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.

73. Quaye, O., Cowins, S., and Gadda, G. (2008) Contribution of FAD C8M Covalent Linkage with His99 to the Reaction Catalyzed by Choline Oxidase, Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.

74. Law, L., Rungsrisuriyachai, K., and Gadda, G. (2008) Preliminary Investigation of the Role of the Conserved, Active Site Asp510 in the Reaction Catalyzed by Choline Oxidase (2008) Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.

75. Rungsrisuriyachai, K., and Gadda, G. (2008) pH Effects on the Steady State Kinetic Mechanism of Pyranose 2-Oxidase from Trametes ochracea, Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.

76. Yuan, H., Sherman, S., and Gadda, G. (2008) Catalytic Importance of the Hydrophilic Side-chain with H-bonding Capability of Ser101 at the Active Site of Choline Oxidase, Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.

77. Francis, K., Chapman, N., and Gadda, G. (2008) Mutation of Histidine 196 to Asparagine in N. crassa 2-Nitropropane Dioxygenase Improves the Catalytic Efficiency of the Enzyme with Anionic Substrates, Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.


78. Finnegan, S., and Gadda, G. (2008) Hydrophobic Packing effects on the Reductive Half-reaction of Choline Oxidase, Second International Interdisciplinary Conference on Vitamins, Coenzymes, and Biofactors, Athens, GA, U.S.A.

79. Yuan, H., and Gadda, G. (2009) The Importance of Serine Serine 101 at the Active Site of Choline Oxidase for Hydroxyl Proton Abstraction, 7th Annual Biotechnology Symposium, Atlanta, GA, U.S.A.

80. Yuan, H., and Gadda, G. (2009) Catalytic Importance of the Hydrophilic Side-chain of Ser101 at the Active Site of Choline Oxidase, the 75th Herty Medal Symposium, Georgia Institute of Technology, Atlanta, GA, U.S.A.

81. Yuan, H., and Gadda, G. (2009) Catalytic Importance of the Hydrophilic Side-chain with H-bonding Capability of Ser101 at the Active Site of Choline Oxidase, Florida Annual Meeting and Exposition 2009, Orlando, FL, U.S.A.

82. Rungsrisuriyachai, K. and Gadda, G. (2009) pH Effect on the Steady State Kinetic Mechanism of Pyranose 2- Oxidase from Trametes ochracea, the 75th Herty Medal Symposium, Georgia Institute of Technology, Atlanta, GA, U.S.A.

83. Rungsrisuriyachai, K. and Gadda, G. (2009) Role of Asn510 in the Relative Timing of Substrate Bond Cleavages in the Reaction Catalyzed by Choline Oxidase, 7th Annual Biotechnology Symposium, Georgia State University, Atlanta, GA, U.S.A.

84. Francis, K., and Gadda, G. (2009) Kinetic Evidence for Nitro Binding Pocket in the Active Site of 2-Nitropropane Dioxygenase, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

85. Francis, K., and Gadda, G. (2009) Branching of an Intermediate Formed during Turnover of N. crassa 2-Nitropropane Dioxygenase Inflates the Observed KIE for the Reaction, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

86. Francis, K., and Gadda, G. (2009) The Observed KIE for the Reaction of 2-Nitropropane Dioxygenase is Inflated Due the Branching of a Reaction Intermediate Formed During Turnover, 75th Herty Medal Symposium, Atlanta, GA, U.S.A.

87. Francis, K., and Gadda, G. (2009) Kinetic Evidence for Nitro Binding Pocket in the Active Site of Nitronate Monooxygenase, 7th Annual Biotechnology Symposium at Georgia State University, Atlanta, GA, U.S.A.

88. Finnegan, S., and Gadda, G. (2009) Choline Oxidase – Overcoming the Spin-forbidden Reaction with Oxygen, 75th Herty Medal Symposium, Atlanta, GA, U.S.A.

89. Finnegan, S., and Gadda, G. (2009) Role of Val464 in the Flavin Oxidation Reaction Catalyzed by Choline Oxidase, 7th Annual Biotechnology Symposium at Georgia State University, Atlanta, GA, U.S.A.

90. Pennati, A. and Gadda, G. (2009) The Effect of pH on the Reductive and Oxidative Half-Reactions Catalyzed by FMN-dependent Human Glycolate Oxidase, 75th Herty Medal Symposium, Atlanta, GA, U.S.A.


91. Pennati, A., and Gadda, G. (2009) pH Effects on the Reaction of Glycolate Oxidation Catalyzed by Human Liver Glycolate Oxidase, 7th Annual Biotechnology Symposium at Georgia State University, Atlanta, GA, U.S.A.

92. Law, L., and Gadda, G. (2009) Engineering a Soluble Variant of Choline Dehydrogenase from Halamonas Elongata, 21st Enzyme Mechanisms Conference, Tucson, AZ, U.S.A.

93. Law, L., and Gadda, G. (2009) Purification of Recombinant Choline Dehydrogenase from Halamonas Elongata, Florida Annual Meeting and Exposition 2009, Orlando, FL, U.S.A.

94. Law, L., and Gadda, G. (2009) Purification of Recombinant Choline Dehydrogenase from Halamonas Elongata, the 75th Herty Medal Symposium, Georgia Institute of Technology, Atlanta, GA, U.S.A.

95. Law, L., and Gadda, G. (2009) Purification of Recombinant Choline Dehydrogenase from Halamonas Elongata, 7th Annual Biotechnology Symposium, Atlanta, GA, U.S.A.

96. Sherman, S., Yuan, H., and Gadda, G. (2009) Importance of the Hydrophilic Side-chain of Serine 101 in the Active Site of Choline Oxidase for Catalysis, 3rd Annual Georgia State Undergraduate Research Conference, Atlanta, GA, U.S.A.

97. Sherman, S., Pennati, A., and Gadda, G. (2009) Purification and Preliminary Characterization of Choline Oxidase from Aspergillus fumigates, 7th Annual Biotechnology Symposium at Georgia State University, Atlanta, GA, U.S.A.

98. Rungsrisuriyachai, K., and Gadda, G. (2010) Role of Asn 510 in the Relative Timing of Substrate Bond Cleavages in the Reaction Catalyzed by Choline Oxidase, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Galveston, TX, U.S.A.

99. Francis, K, and Gadda, G. (2010) Temperature and Kinetic Isotope Effects on the Nitronate Monooxygenase Catalyzed reaction, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Galveston, TX, U.S.A.

100. Finnegan, S., and Gadda, G. (2010) The Nature of the Kinetically Slow Equilibrium Between Two Conformers of the Val464Ala Variant of Choline Oxidase, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.

101. Francis, K., and Gadda, G. (2010) A Novel Function of Nitronate Monooxygenase: Detoxification of the Plant Toxin Propionate-3-Nitronate, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.

102. Gannavaram, S., and Gadda, G. (2010) Kinetic Isotope Effect of Choline Oxidase Wild Type from Arthrobacter globiformis, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.

103. Law, L., and Gadda, G. (2010) Investigation of D-Arginine Dehydrogenase Tyr249 Mutants, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.

104. Pennati, A., and Gadda, G. (2010) Stabilization of a Flavosemiquinone in the Oxidative Half Reaction of Human Liver Glycolate Oxidase, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.


105. Rungsrisuriyachai, K., and Gadda, G. (2010) Role of Asparagine 510 in the Relative Timing of Substrate Bind Cleavages in the Reaction Catalyzed by Choline Oxidase, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.

106. Francis, K., and Gadda, G. (2010) A Novel Function of Nitronate Monooxygenase: Detoxification of the Plant Toxin Propionate-3-Nitronate, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

107. Smitherman, C., Rungsrisuriyachai, K., and Gadda, G. (2011) Is His466 the Catalytic Base for Choline Oxidase from Arthrobacter globiformis?, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

108. Gannavaram, S., Law, L., Yuan, H., Fu, G., Weber, I., and Gadda, G. (2011) Role of Tyrosine 249 in substrate binding of D-Arginine Dehydrogenase from Pseudomonas aeruginosa, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

109. Francis, K., Nishino, S., Spain, J. and Gadda, G. (2011) A Novel Activity for Fungal Nitronate Monooxygenase: Detoxification of the Metabolic Inhibitor Propionate-3-Nitronate, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

110. Pennati, A., Lambou, K., J-P., Latge, and Gadda, G. (2011) Choline-Glycine Betaine Metabolic Pathway in Aspergillus fumigatus, Southeast Enzyme Conference 2011, Atlanta, GA, U.S.A.

111. Yuan, H., and Gadda, G. (2011) On the Catalytic Mechanism of D-Arginine Dehydrogenase, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

112. Smitherman, C., Francis, K., Nishino, S., Spain, J., and Gadda, G. (2011) Detoxification of a Lethal Toxin by Nitronate Monooxygenase, Seventeenth International Symposium on Flavins and Flavoproteins, Berkeley, CA, U.S.A.

113. Gannavaram, S., and Gadda. G. (2011) Role of Tyrosine249 in Substrate Binding of D-Arginine Dehydrogenase from Pseudomonas aeruginosa, Second Southeast Enzyme Conference, Atlanta, GA, U.S.A.

114. Pennati, A., and Gadda. G. (2011) Choline-Glycine Betaine Metabolic Pathway in Aspergillus fumigatus, Second Southeast Enzyme Conference, Atlanta, GA, U.S.A.

115. Uluisik, R., and Gadda. G. (2011) Effect of Temperature on the Kinetic Isotope Effects of the Active Site Variant S101A of Choline Oxidase, Second Southeast Enzyme Conference, Atlanta, GA, U.S.A.

116. Smitherman, C., and Gadda. G. (2011) Is a Catalytic Base Required for Choline Activation in Choline Oxidase from Arthrobacter globiformis, Second Southeast Enzyme Conference, Atlanta, GA, U.S.A.

117. Smitherman, C., Francis, K., and Gadda, G. (2011) Detoxification of a Lethal Toxin by Nitronate Monooxygenase, 17th International Symposium on Flavins and Flavoproteins, Berkeley, CA, U.S.A.

118. Gadda, G. (2011) Oxygen Activation in Choline Oxidase: the Value of Positive Substrates, Seventeenth International Symposium on Flavins and Flavoproteins, Berkeley, CA, U.S.A.


119. Smitherman, C., and Gadda, G. (2012) Secondary Kinetic Isotope Effects as Probes to Investigate Catalysis in Nitronate Monooxygenase, Gordon Research Conference on Isotopes in the Biological and Chemical Sciences, Galveston, TX, U.S.A.

120. Uluisik, R., and Gadda, G. (2012) Importance of a Serine Proximal to the C(4) and N(5) Flavin Atoms for Quantum Mechanical Tunneling of the Hydride in Choline Oxidase, Gordon Research Conference on Isotopes in the Biological and Chemical Sciences, Galveston, TX, U.S.A.

121. Salvi, F., and Gadda, G. (2012) Importance of the Hydrophobic Cluster Controlling Substrate Access for the Hydride Transfer Reaction Catalyzed by Choline Oxidase. Gordon Research Conference on Isotopes in the Biological and Chemical Sciences, Galveston, TX, U.S.A.

122. Gannavaram, S., and Gadda. G. (2012) Kinetic Determination of Redox Potential for Flavin Reduction in D-Arginine Dehydrogenase, Gordon Research Seminar on Isotopes in Biological and Chemical Sciences, Galveston, TX, U.S.A.

123. Smitherman, C., and Gadda. G. (2012) On the Catalytic Mechanism of Nitronate Monooxygenase, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

124. Gannavaram, S., and Gadda. G. (2012) Relative Timing of Proton Transfers in the Reduction of Oxygen in Choline Oxidase from Arthrobacter globiformis, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

125. Uluisik, R., and Gadda. G. (2012) The role of S101 in Quantum Mechanical Hydride Tunneling in Choline Oxidase, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

126. Salvi, F., and Gadda. G. (2012) Role of the Hydrophobic Cluster in the Hydride Transfer Reaction Catalyzed by Choline Oxidase, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

127. Francis, K., Nishino, S., Smitherman, C., Spain, J., and Gadda. G. (2012) Detoxification of the Metabolic Toxin Propionate-3-Nitronate by Fungal Nitronate Monooxygenase, The Florida Annual Meeting and Exposition, Tampa, FL, U.S.A.

128. Gannavaram, S., and Gadda, G. (2012) Relative Timing of Proton Transfers in Oxygen Reduction Catalyzed by Choline Oxidase, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

129. Santos, Y.J., Smitherman, C., and Gadda, G. (2012) Purification of a Detoxifying Enzyme from Williopsis saturnus, Herty Medalist Undergraduate Research Symposium, Atlanta, GA, U.S.A.

130. Bui, Q.V., Gebrehiwot, E.B., Gannavaram, S., and Gadda, G. (2012) Comparison of Kinetic Parameters of D-Arginine Dehydrogenase Using Different Substrates as a Function of pH, Herty Medalist Undergraduate Research Symposium, Atlanta, GA, U.S.A.

131. Ball, J., Gannavaram, S., and Gadda, G. (2012) pH Effects on the Steady-State Turnover of D-Arginine Dehydrogenase with D-Arginine as Substrate, Herty Medalist Undergraduate Research Symposium, Atlanta, GA, U.S.A.

132. Gannavaram, S., and Gadda, G. (2013) Mechanism of Flavin oxidation in Choline Oxidase, 23rd Enzyme Mechanisms Conference, Coronado Bay, California, U.S.A.


133. Bui, Q., Gannavaram, S., and Gadda, G. (2013) pH and Solvent Viscosity Effects of D-Arginine Dehydrogenase with Different D-Amino Acid Substrates, Georgia State University Undergraduate Research Conference, Atlanta, GA, U.S.A.

134. Ball, J., Gannavaram, S., and Gadda, G. (2013) Investigation of Ionizable Groups that Participate in D-Arginine Dehydrogenase Catalysis and Binding, Georgia State University Undergraduate Research Conference, Atlanta, GA, U.S.A.

135. Smitherman, C., and Gadda, G. (2013) An Ongoing Journey to Crystallize a Detoxifying Enzyme, Nitronate Monooxygenase from Williopsis saturnus, Fourth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

136. Santos, Y.J., Smitherman, C., and Gadda, G. (2013) Investigation of Substrate Oxidation in Choline Oxidase through Further Mutagenesis of Histidine Residues., Fourth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

137. Salvi, F., and Gadda, G. (2013) Kinetic characterization of the mutant enzyme CHO-M62A/F357A of Choline Oxidase for the investigation of Flavin Oxidation, Fourth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

138. Bui, Q., Gannavaram, S., and Gadda, G. (2013) pH Profiles of D-Arginine Dehydrogenase with D-Histidine and D-Methionine, Fourth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

139. Ball, J., Gannavaram, S., and Gadda, G. (2013) Kinetic Analysis of D-Arginine Dehydrogenase by pH and Inhibition Effects, Fourth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

140. Ouedraogo, D., Salvi, F. and Gadda, G. (2013) Mutagenesis, Expression, Purification, and Characterization of the D-Arginine Dehydrogenase Variant, I335H, Fourth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

141. Romero, E., and Gadda, G. (2013) Effect of temperature on the reductive-half reaction of human glycolate oxidase, Fourth Annual Southeast Enzyme Conference, U.S.A.

142. Gannavaram, S., and Gadda, G. (2013) Characterization of a Green Flavin from D-Arginine Dehydrogenase. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

143. Romero, E., and Gadda, G. (2013) Temperature and Solvent Viscosity Dependence of the Reductive-half Reaction of Human Glycolate Oxidase. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

144. Smitherman, C., Rungsrisuriyachai, K., Germann, M.W., and Gadda, G. (2013) Mechanistic Studies of Choline Oxidase H466Q. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.



146. Romero, E., and Gadda, G. (2013) Evidence for hydride tunneling in the human glycolate oxidase reaction: A stopped-flow study at various temperatures, 65th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

147. Ball, J., Su, D., and Gadda, G. (2013) Chemical modification and site-directed mutagensis of His48 in D-Arginine Dehydrogenase, 65th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

148. Salvi, F., Wang, Y.F., Weber, I.T., and Gadda, G. (2013) Crystal structure of choline oxidase from Arthrobacter globiformis in complex with glycine betaine suggests the importance of the dimer interface for substrate access to the active site, 65th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

149. Romero, E., and Gadda, G. (2014) Kinetic Isotope Effects in Human Glycolate Oxidase: Evidences for Hydride Tunneling. Gordon Research Conference on Isotopes in the Biological and Chemical Sciences, Galveston, TX, U.S.A.

150. Bui, Q., Salvi, F., and Gadda, G. (2014) Steady-state kinetics of nitronate monoxygenase from Pseudomonas aeruginosa (PA4202), Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

151. Romero, E., and Gadda, G. (2014) Involvement of hydride tunneling and dynamics in the reductive half-reaction of human glycolate oxidase, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

152. Salvi, F., Agniswamy, J., Yuan, H., Vercammen, K., Pelicaen, R., Cornelis, P., Spain, J., Weber, I.T. and Gadda, G. (2014) The combined structural and kinetic characterization of a bacterial nitronate monooxygenase from Pseudomonas aeruginosa PAO1 establishes a class of enzymes, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

153. Ball, J., Gannavaram, S., and Gadda, G. (2014) pH dependence, chemical modification, and site-directed mutagenesis of PaDADH: Implications for a catalytic base, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

154. Su, D., Francis, K., and Gadda, G. (2014) Investigation on the catalytic mechanism of flavin-dependent propionate-3-nitronate monooxygenase from Pseudomonas sp. Strain JS189, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

155. Gannavaram, G., Sirin, S., and Gadda, G. (2014) Mechanistic and computational studies on C-N bond oxidation catalyzed by D-arginine dehydrogenase, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

156. Ouedraogo, D., and Gadda, G. (2014) Mechanistic investigation of D-arginine dehydrogenase variant, I335H, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

157. Hernandez, J., Smitherman, C., Salvi, F., and Gadda, G. (2014) Strategies for enhancing recombinant expression of bacterial nitronate monooxygenase, Georgia-Alabama Louis Stokes Alliances for Minority Participation (LSAMP) Spring Symposium, Atlanta, GA, U.S.A.


158. Hernandez, J., Smitherman, C., Salvi, F., and Gadda, G. (2014) Optimizing the expression of nitronate monooxygenase in Escherichia coli. Georgia State Undergraduate Research Conference, Atlanta, GA, U.S.A.

159. Hernandez, J., Smitherman, C., Salvi, F., and Gadda, G. (2014) Effective induction conditions for the recombinant expression of bacterial nitronate monooxygenase, Fifth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

160. Smitherman, C., and Gadda, G. (2014) Effects of solvent viscosity on the reaction catalyzed by nitronate monooxygenase from Cyberlindnera saturnus, Fifth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

161. Ouattara, M., Sachla, A.J., Romero, E., Agniswamy, J., Weber, I.T. and Gadda, G. (2014) Identification and characterization of HupZ: the first heme-degrading enzyme from Streptococcus sp, Fifth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

162. Salvi, F., Agniswamy, J., Yuan, H., Vercammen, K., Pelicaen, R., Cornelis, P., Spain, J., Weber, I.T., and Gadda, G. (2014) The Combined Structural and Kinetic Characterization of a Bacterial Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1 Establishes a Class of Enzymes. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

163. Smitherman, C., and Gadda, G. (2014) Product Determination from the Enzymatic Detoxification of Propionate 3-Nitronate by Nitronate Monooxygenase. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

164. Smitherman, C., Salvi, F., Su, D., Hudson, B., and Gadda, G. (2015) Class I Nitronate Monooxygenases: a New Family of Detoxifying Enzymes, 24th Enzyme Mechanisms Conference, Galveston, TX.

165. Smitherman, C. and Gadda, G. (2015) “Excited State Proton Transfers in Flavoprotein Oxidases.” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

166. Ball, J., Salvi, F., and Gadda, G. (2015) “NADH:quinone Reductase Activity of PA1024 from Pseudomonas aeruginosa”, Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

167. Smitherman, C. and Gadda, G. (2015) “Oxidative Detoxification of Propionate 3-nitronate by Nitronate Monooxygenase and Novel Detection of Enzymatic Products.” Sixth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

168. Ball, J., Salvi, F., and Gadda, G. (2015) “NADH:Quinone Reductase Activity of PA1024 from Pseudomonas aeruginosa .” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, U.S.A.

169. Ball, J., Salvi, F., and Gadda, G. (2015) “The Kinetic Characterization of PA1024 from Pseudomonas aeruginosa Establishes the Enzyme as an NADH:menadione Reductase and not a Nitronate Monooxygenase.” Sixth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.


170. Su, D. and Gadda, G. (2015) “Mechanistic and Structural Study of Bacterial Propionate 3-nitronate from Pseudomonas sp. Strain JS189.” Sixth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

171. Su, D. and Gadda, G. (2015) “Spectroscopic and Kinetic Study on Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

172. Ouedraogo, D., Vasquez, S., and Gadda, G. (2015) “Kinetic and Biophysical Investigations of Residues Surrounding the Flavin in D-arginine Dehydrogenase from Pseudomonas aeruginosa” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

173. Ouedraogo, D., and Gadda, G. (2014) “Mechanistic Investigation of D-Arginine Dehydrogenase Variant, I335H” Sixth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

174. Aguillon, C. and Gadda, G. (2015) “Probing the Role of Tyrosine 254 in Class I Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1 by Site-Directed Mutagenesis.” Molecular Basis of Disease Undergraduate Fellowship Luncheon, Atlanta, GA, U.S.A.

175. Flores, E., Su, D., and Gadda, G. (2016) “Characterization of a Putative NAD(P)H-Quinone Oxidoreductase in Pseudomonas aeruginosa Strain PAO1.” Southeast Undergraduate Research Conference, Atlanta, GA, U.S.A.

176. Iyer, A., Aguillon, C., Ball, J., Flores, E., Ouedraogo, D., Su, D., Tofighi, H., and Gadda, G. (2016) “GADDA Research Group.” Southeast Undergraduate Research Conference, Atlanta, GA, U.S.A.

177. Aguillon, C., Su, D., and Gadda, G. (2016) “Elucidating the Role of Select Residues through Site-Directed Mutagenesis in Class I Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

178. Aguillon, C., Su, D., and Gadda, G. (2016) “On the Role of Tyrosine 254 in Class I Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” GA-AL LSAMP 2016 Annual Research Scientists of Tomorrow Symposium, Atlanta, GA, U.S.A.

179. Ball, J., Salvi, F., and Gadda, G. (2016) “Spectroscopic and Kinetic Characterization of an FMN-dependent Quinone Reductase.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

180. Flores, E., Su, D., and Gadda, G. (2016) “Purification and Characterization of a Putative NAD(P)H-Quinone Oxidoreductase in Pseudomonas aeruginosa Strain PAO1.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

181. Flores, E., Su, D., and Gadda, G. (2016) “Characterization of Recombinant PA1225: a Hypothetical NAD(P)H-Quinone Oxidoreductase from Pseudomonas aeruginosa PAO1.” 10th Annual Georgia State University Undergraduate Research Conference, Atlanta, GA, U.S.A.


182. Flores, E., Su, D., and Gadda, G. (2016) “Purification and Characterization of a Putative NAD(P)H-Quinone Oxidoreductase in Pseudomonas aeruginosa Strain PAO1.” GA-AL LSAMP 2016 Annual Research Scientists of Tomorrow Symposium, Atlanta, GA, U.S.A.

183. Iyer, A., Gannavaram, S., Spring, A., Germann, M.W., and Gadda, G. (2016) “Characterization of the 6-Hydroxy-FAD of Y249F variant of D-Arginine Dehydrogenase from Pseudomonas aeruginosa.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

184. Ouedraogo, D., Vasquez, S., and Gadda, G. (2016) “Insights on the Role of Ser/Ala Switch in Amine Oxidation in Pseudomonas aeruginosa D-Arginine Dehydrogenase.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

185. Su, D. and Gadda, G. (2016) “Mechanistic and Spectroscopic Study of Bacterial Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

186. Tofighi, H., Su, D., Aguillon, C., and Gadda, G. (2016) “Combined Mutagenesis and Kinetic Characterization of Bacterial Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

187. Aguillon, C., Su, D., and Gadda, G. (2016) “Kinetic and Spectroscopic Variants from Pseudomonas aeruginosa.” Molecular Basis of Disease and NSF-REU Summer Undergraduate Research Luncheon, Atlanta, GA, U.S.A.

188. Ball,J., Salvi, F., Felix, T., and Gadda, G. (2016) “A New Class of NADH:quinone Reductases.” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

189. Felix, T., Ball, J., and Gadda, G. (2016) “Mutagenesis of a Predicted Catalytic Lysine in the Active Site of PA1024.” Molecular Basis of Disease and NSF-REU Summer Undergraduate Research Luncheon, Atlanta, GA, U.S.A.

190. Gadda, G., Su, D., Tofighi, H., and Kwok, M. (2016) “Direct Observation of Anionic and Neutral Flavosemiquinones during Catalysis of a Monooxygenase.” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

191. Kwok, M., Tofighi, H., Gadda, G. (2016) “Protein Expression and Purification of Nitronate Monooxygenase from Pseudomonas aeruginosa.” Molecular Basis of Disease and NSF-REU Summer Undergraduate Research Luncheon, Atlanta, GA, U.S.A.

192. Ouedraogo, D., Vasquez, S., Souffrant, M., Waiters, S., Marshall, S., Coleman, M., Adodo, E., Mariani, V., Hamelberg, D., and Gadda, G. (2016) “Kinetic and Biophysical Investigations of Ser/Ala Switch and Glu 246 in D-Arginine Dehydrogenase from Pseudomonas aeruginosa.” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

193. Su. D. and Gadda, G. (2016) “Mechanistic Study of Bacterial Propionate 3-Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” Molecular Basis of Disease Graduate Research Day, Atlanta, GA, U.S.A.


194. Iyer, A., Reis, R., Gannavaram, S., Spring, A., Germann, M.W., Agniswamy, J., Weber, I.T., and Gadda., G. (2017), 25th Enzyme Mechanisms Conference, St. Pete Beach, FL, U.S.A.

195. Flores, E., and Gadda, G. (2017), 25th Enzyme Mechanisms Conference, St. Pete Beach, FL, U.S.A.

d) INSTRUCTION AND ADVISING

d.i) Courses Taught at GSU

1. Principles and Techniques of Practical Biochemistry (CHEM 4670/6670; BIOL 4670/6670 - offered to undergraduate and graduate students): Fall 2013, Fall 2014, Fall 2015, Fall 2016. This course has was proposed and developed by Dr. Giovanni Gadda and was offered for the first time in Fall 2013.

2. Advanced Topics in Biochemistry – Principles and Techniques of Practical Biochemistry (Chem 8620 – offered to PhD students in Chemistry and Biology): Fall 2009; Fall 2012.

3. Biochemistry I (CHEM 4600/6600 – offered to undergraduate and graduate students): Fall 2000; Spring 2001; Fall 2001; Fall 2002; Fall 2003; Spring 2004; Spring 2005; Fall 2006.

4. Biochemistry I (CHEM 6600 - offered to graduate students): Fall 2007; Fall 2008; Fall 2010; Fall 2011.

5. Enzymology (CHEM 4630/6630; BIOL 4630/6630 – offered to undergraduate and graduate students): Spring 2002; Spring 2003; Fall 2004; Fall 2005; Spring 2007; Spring 2008; Spring 2009; Spring 2010; Spring 2011; Spring 2012; Spring 2013; Spring 2014; Spring 2015; Spring 2016, Spring 2017. This course was proposed and developed by Dr. Giovanni Gadda and was offered at Georgia State University for the first time in the Spring Semester 2002.

6. Advanced Research Methods (CHEM 4900/6900 - offered to undergraduate and graduate students): Spring 2005; Fall 2005; Spring 2006; Fall 2006; Spring 2007; Fall 2007; Spring 2008; Fall 2008; Spring 2009; Fall 2009; Spring 2010; Summer 2010; Fall 2010; Spring 2011; Summer 2011; Fall 2011; Spring 2012; Summer 2012; Fall 2012; Spring 2013; Summer 2013; Fall 2013; Spring 2014, Summer 2014, Fall 2014, Spring 2015, Summer 2015, Fall 2015, Spring 2016, Summer 2016, Fall 2016, Spring 2017.

7. From Fall 2000 to current: numerous times for: Directed Study in Chemistry (CHEM 8900 - offered to graduate students); Directed Research in Chemistry (CHEM 8910 - offered to graduate students); Thesis Research (CHEM 8999 - offered to graduate students); Dissertation Research (CHEM 9999 - offered to graduate students); Non-thesis Paper Writing (CHEM 8888 - offered to graduate students); Chemical Research (CHEM 4950 - offered to undergraduate students); Chemistry Laboratory IVA (CHEM 4160 - offered to undergraduate students); Chemistry Laboratory IVB (CHEM 4170 - offered to undergraduate students); Undergraduate Research in Biology (BIOL 4910 - offered to undergraduate students).


8. Instructor at the NSF Center for Workshop in the Chemical Sciences – Molecular Genetics and Protein Structure/Function Techniques, Georgia State University August 2001, August 2002 (http://chemistry.gsu.edu/CWCS/genetics.html).

d.ii) Research Advisor at Georgia State University (Past)

1. John T. Kanazawa, Undergraduate Student in Chemistry, January 2001 to September 2001 (Bachelor in Science).

2. Bethany Russell, Undergraduate Student in Chemistry, January 2001 to December 2001 (Bachelor in Science); Master of Science in Chemistry, January 2002 to December 2002. (Master of Science).

3. Katina L. Johnson, Undergraduate Student in Chemistry, January 2001 to December 2001 (Bachelor in Science); Master of Science Student in Chemistry, January 2002 to August 2002.

4. Elien McAllister, Undergraduate Student in Chemistry, January 2001 to December 2001 (Bachelor in Science); Master of Science in Chemistry, January 2002 to December 2002. (Master of Science).

5. Dawit Seyfe, Undergraduate Student in Biology, August 2001 to June 2002. (Bachelor in Science); McNair Post-Baccalaureate Fellow (Chemistry), May 2001 ad August 2001.

6. Thaddeus Harvey II, McNair Post-Baccalaureate Fellow (Biology), May 2001 to August 2001.

7. Brittany Bivines, McNair Post-Baccalaureate Fellow (Biology), June 2002 to August 2002.

8. Sandra Imarah, McNair Post-Baccalaureate Fellow (Biology), June 2002 to August 2002.

9. Aisha Tolbert, Bridges to the Future Fellow (Chemistry), May 2003 to July 2003.

10. Kevin Francis, McNair Post-Baccalaureate Fellow (Biology), June 2003 to August 2003; Undergraduate in student in Biology January 2003 to August 2004.

11. Tranbao N’Guyen, McNair Post-Baccalaureate Fellow (Chemistry), June 2003 to August 2003; Undergraduate in Chemistry, August 2003 to December 2005.

12. Nichole L. N. Powell, Ph.D. Student in Chemistry, March 2001 to December 2003.

13. Bhavisha Patel, Undergraduate in Biology, August 2003 to August 2004.

14. Prashanthi Menon, Master of Science in Biology, May 2003 to May 2004.

15. Fan Fan, Ph.D. Student in Biology, August 2001 to December 2005.

16. Thuy-Trang Hoang, Undergraduate Student in Chemistry, January 2004 to August 2005 (Bachelor in Science)

17. Mahmoud Ghanem, Ph.D. Student in Chemistry, May 2002 to May 2006.

http://chemistry.gsu.edu/CWCS/genetics.html


18. Jane (Thuy-Trang) V. Hoang, Master of Science in Chemistry, August 2005 to August 2006.

19. Anthony Mgbodile, McNair Post-Baccalaureate Fellow, May 2006 to August 2006.

20. Slavica Mijatovic, Undergraduate Student in Chemistry, January 2006 to August 2006.

21. Kevin Francis, Master of Science in Chemistry, August 2004 to May 2007.

22. Merid Belaineh, Undergraduate Student in Chemistry, and McNair Post-Baccalaureate Fellow (Chemistry), June 2005 to December 2006.

23. Stacy Collins, Undergraduate Student in Biology, March 2007 to August 2007.

24. Slavica Mijatovic, Master of Science in Chemistry, August 2006 to May 2008.

25. Tranbao Nguyen, Master of Science in Chemistry, August 2006 to December 2007.

26. Sharonda Cowins, Summer Intern, BS Student at Albany State University, May 2008 to August 2008.

27. Danila Crobu, PhD exchange-student from University of Milano, Milan, February 2009 to April 2009.

28. Nicole Chapman, BS Student in Chemistry, August 2008 to May 2009.

29. Phillip Brook, BS in Chemistry, January 2009 to May 2009.

30. Osbourne Quaye, Ph.D. Student in Chemistry, January 2005 to August 2009.

31. Samuel Alejos, BS in Biology, May 2009 to December 2009.

32. Kunchala Rungsrisuriyachai, Ph.D. Student in Chemistry, January 2005 to May 2010.

33. Steffan Finnegan, Ph.D. Student in Chemistry, January 2006 to May 2010.

34. Lydia Law, MS Student in Chemistry, August 2008 to August 2010.

35. Stephen Sherman, BS Student in Chemistry, August 2008 to May 2010.

36. Peter Graf, BS Student in Chemistry, May 2010 to December 2010.

37. Kevin Francis, Ph.D. Student in Chemistry, August 2006 to May 2011.

38. Dr. Andrea Pennati, Post-Doctoral Research Associate, May 2007 to June 2011.

39. Hongling Yuan, Ph.D. Student in Chemistry, August 2006 to August 2011.

40. Rizvan Uluisik, BS Student in Biology, August 2010 to August 2011.

41. Dr. Hongling Yuan, Post-Doctoral Research Associate, August 2011 to October 2011.

42. Sandra Hagans, BS Student in Biology, January 2001 to December 2011.


43. Eled Brehanu Gebrehiwot, Summer Intern, BS Student at Georgia Perimeter College, May 2012 to August 2012.

44. Quan Vo Van Bui, Summer Intern, BS Student at Georgia Perimeter College, May 2012 to August 2012.

45. Alexandria Smith, Summer Intern Project SEED ACS, High Schooler, June 2012 to August 2012.

46. Daniel Ouedraogo, Post-Bacchalaureate, June 2012 to August 2012.

47. Yvette Santos, BS Student in Communication, May 2012 to May 2013.

48. Rizvan Uluisik, MS Student in Chemistry, August 2011 to April 2013.

49. Jacob Ball, BS Student in Chemistry, May 2012 to August 2013.

50. Quan Vo Van Bui, BS Student in Chemistry, August 2012 to December 2013.

51. Jennifer Hernandez, BS Student in Chemistry, August 2013 to May 2014.

52. Swathi Gannavaram, Ph.D. Student in Chemistry, August 2009 to May 2014.

53. Folashade Maxwell-Shittu, BS Student NSF-STEP (3+8) funded, May 2014 to August 2014.

54. Brianna Hudson, BS Student NSF-REU funded, June 2014 to August 2014.

55. Jacob Ball, MS Student in Chemistry, August 2013 to December 2014.

56. Elvira Romero-Guzman, Postdoctoral Research Associate, August 2012 to December 2014.

57. Brianna Hudson, BS Student at Agnes Scott, Atlanta, volunteer, August 2014 to December 2014.

58. Quan Vo Van Bui, MS Student in Chemistry, January 2014 to February 2015.

59. Francesca Salvi, Ph.D. Student in Chemistry, August 2011 to April 2015.

60. Sheena Vasquez, BS Student at Georgia Perimeter College, Atlanta, volunteer, August 2014 to May 2015.

61. Rachel Kyeremaa, B.S. Student at GPC, May 2015 to August 2015.

62. Francine Elle, B.S. Student at GPC, June 2015 to August 2015.

63. Crystal Smitherman, Ph.D. Student in Chemistry, August 2010 to December 2015.

64. Juan Rogelio Carro Arumburo, PhD exchange-student from Consejo Superior de Investigaciones Científicas, Madrid, September 2015 to December 2015.

65. Daniel Ouedraogo, MS Student in Chemistry, August 2012 to December 2015.


66. Thomas Felix, BS Student from North Georgia College NSF-REU funded, May 2016 to August 2016.

67. Miyuki Kwok, BS Student from Hong Kong Baptist University (summer exchange program), June 2016 to August 2016.

68. Chuck Harrell, B.S. Student at GPC, June 2016 to August 2016.

69. Elias Flores, B.S. Student in Chemistry, August 2015 to May 2016.

d.iii) Research Advisor at Georgia State University (Present)

1. Dan Su, Ph.D. Student in Chemistry, August 2012 to present.

2. Jacob Ball, PhD Student in Chemistry, January 2015 to present.

3. Hossein Tofighi, Ph.D. Student in Chemistry, August 2015 to present.

4. Daniel Ouedraogo, PhD Student in Chemistry, January 2016 to present.

5. Archana Iyer, PhD Student in Chemistry, January 2016 to present.

6. Christopher Aguillon, B.S. Student in Chemistry, May 2015 to present.

7. Elias Flores, M.S. Student in Chemistry, June 2016 to present.

8. Maria Vodovoz, BS Student in Chemistry, May 2016 to present.

9. Renata Garcia Almeida Reis, Postdoctoral Associate, July 2016 to present.

10. Andrew Baumert, M.S. Student in Chemistry, August 2016 to present.

d.iv) Students Awards

2017 Crystal Smitherman; elected Chair of the Gordon Research Seminar on Enzymes, Coenzymes and Metabolic Pathways.

2017 Jacob Ball; Molecular Basis Diseases Fellowship from Georgia State University.

2017 Dan Su; Molecular Basis Diseases Fellowship from Georgia State University.

2017 Christopher Aguillon; Initiative for Maximizing Student Development from the Neuroscience Institute of Georgia State University (NIH Funds).

2017 Christopher Aguillon; Louis Stokes Alliance for Minority Participation Fellowship (LSAMP).

2017 Christopher Aguillon; University Assistantship from the Honors College of Georgia State University and the Neuroscience Institute of Georgia State University.


2016 Jacob Ball; Molecular Basis Diseases Fellowship from Georgia State University.


2016 Christopher Aguillon; Louis Stokes Alliance for Minority Participation Fellowship (LSAMP).

2016 Christopher Aguillon; Initiative for Maximizing Student Development from the Neuroscience Institute of Georgia State University (NIH Funds).

2016 Christopher Aguillon; Undergraduate Molecular Basis of Disease Fellowship from Georgia State University.

2016 Crystal Smitherman; elected Vice-Chair of the Gordon Research Seminar on Enzymes, Coenzymes and Metabolic Pathways.

2015 Elias Flores; Louis Stokes Alliance for Minority Participation Fellowship (LSAMP).

2015 Christopher Aguillon; Louis Stokes Alliance for Minority Participation Fellowship (LSAMP)

2015 Crystal Smitherman; Center for Diagnostics and Therapeutics Fellowship from Georgia State University.


2015 Crystal Smitherman; Jeremy Knowles Travel Award by the Division of Biological Chemistry of the American Chemical Society at the Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways.

2015 Christopher Aguillon; Undergraduate Molecular Basis Diseases Fellowship from Georgia State University.

2015 Sheena Vasquez: Physical Science Award at the Georgia Perimeter College, GPC.

2015 Francesca Salvi: 2014 Graduate Award for Outstanding Research at the Doctoral Level, GSU Department of Chemistry.

2015 Crystal Smitherman: 2014 Chair's Award, GSU Department of Chemistry.

2014 Francesca Salvi; Molecular Basis Diseases Fellowship from Georgia State University.


2014 Jennifer Hernandez; Louis Stokes Alliance for Minority Participation Fellowship (LSAMP).

2014 Swati Gannavaram; Molecular Basis of Disease Travel Award from Georgia State University to attend the National Meeting of the ASBMB in San Diego, CA.


2014 Swati Gannavaram; ASBMB Travel Award to attend the National Meeting of the ASBMB in San Diego, CA.


2013 Swati Gannavaram; 1st Prize for Oral Presentation at Molecular Basis for Disease Research Day, Atlanta, GA.

2013 Swati Gannavaram; Travel Award from Molecular Basis of Disease to attend the 23rd Enzyme Mechanisms Conference, Coronado Bay, CA.

2013 Swati Gannavaram; Molecular Basis Diseases Fellowship from Georgia State University.

2013 Jacob Ball; Undergraduate Molecular Basis Diseases Fellowship from Georgia State University.

2013 Quan Bui; Undergraduate Molecular Basis Diseases Fellowship from Georgia State University.

2012 Swati Gannavaram; 2nd Prize for Poster Presentation at Molecular Basis for Disease Research Day, Atlanta, GA.

2012 Swati Gannavaram: Selected for oral presentation at Gordon Research Seminar (GRS) on Isotopes in Biological and Chemical Science, Galveston, TX.

2012 Francesca Salvi: Graduate Teaching Award, Doctoral Level.

2012 Yvette, Santos; Provost University Assistantship (August 2012 - May 2013)


2012 Jacob Ball; Undergraduate Molecular Basis Diseases Fellowship from Georgia State University.

2011 Francesca Salvi: Graduate Teaching Award, Doctoral Level.

2011 Sandra Hagans; Louis Stokes Alliance for Minority Participation Fellowship.


2011 Sandra Hagans; Molecular Basis of Disease Summer Undergraduate Research Fellowship.

2011 Kevin Francis; 2010 Chair’s Award.

2011 Hongling Yuan; 2010 Graduate Award for Outstanding Research at the Doctoral Level.

2010 Kevin Francis; Molecular Basis Diseases Fellowship from Georgia State University.


2009 Kevin Francis; 2009 Graduate Award for Outstanding Research at the Doctoral Level.

2009 Kunchala Rungsrisuriyachai; 2009 Graduate Teaching Award.

2009 Steffan Finnegan; Molecular Basis Diseases Fellowship from Georgia State University.

2009 Steffan Finnegan; 2008 Chair’s Award.


2009 Phillip Brooks; Biotechnology Scholar Fellowship from Georgia State University.

2009 Nichole Chapman; Biotechnology Scholar Fellowship from Georgia State University.


2008 Kevin Francis; Ambrose H. Pendergrast Fellowship.


2008 Phillip Brooks; Award for Oral Presentation at the Annual Biomedical Research Conference for Minority Students (ABRCMS), Orlando, FL.



2006 Mahmoud Ghanem; Pendergrast Fellowship from Chemistry Department, GSU.

2005 Fan, Fan; Molecular Basis Diseases Fellowship from Georgia State University.

2005 Fan, Fan ; Graduate Award for Outstanding Research from Chemistry Department of Georgia State University.

2004 Fan, Fan; Molecular Basis Diseases Fellowship from Georgia State University.

2001 Katina Johnson; AMP Scholar Fellowship from Georgia State University.

2001 Elizabeth McAllister; AMP Scholar Fellowship from Georgia State University.

2001 Bethany Russell; AMP Scholar Fellowship from Georgia State University.


d.v) Students Seminar Presentations (total: 34)

1. Powell, N., and Gadda, G. (2001) Cloning and Expression of the betA gene from Escherichia coli encoding choline dehydrogenase, 53rd Southeast Regional Meeting of the American Chemical Society, Savannah, GA, U.S.A.

2. Russell, B., McAllister, E., and Gadda, G. (2001) Cloning and Expression of the FAD-Containing Choline Dehydrogenase From Different Cellular Sources, November 2nd, Department of Chemistry, Georgia State University, Atlanta, GA, U.S.A.

3. Powell, N., Imarah, S., and Gadda, G. (2002) Cloning, Expression, Purification, and Kinetic Parameters of Choline Dehydrogenase from Escherichia coli, 54th Southeast Regional Meeting of the American Chemical Society, Charleston, South Carolina, U.S.A.

4. Russell, B., Johnson, K.L., Bivines, B.J., and Gadda, G. (2002) Biochemical and Kinetic Characterization of 2-Nitropropane Dioxygenase from Neurospora crassa, 54th Southeast Regional Meeting of the American Chemical Society, Charleston, South Carolina, U.S.A.

5. Fan, F., Ghanem, M., and Gadda, G. (2002) Cloning, Expression and Purification of Choline Oxidase from Arthrobacter globiformis, 54th Southeast Regional Meeting of the American Chemical Society, Charleston, South Carolina, U.S.A.

6. Fan, F., and Gadda, G. (2003) Substrate Kinetic Isotope Effects Studies of Choline Oxidase: Implications for the Relative Timing of Substrate CH and OH Bond Cleavage, 55th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

7. Francis, K., Ghanem, M., Powell, N., and Gadda, G. (2003) Characterization of the Active Site Topology of Choline Oxidase, 55th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

8. Ghanem, M., and Gadda, G. (2003) Biochemical and Kinetic Properties of Choline Oxidase Active Site Mutant His-466-Ala, 55th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

9. Hoang, T. V., and Gadda, G. (2006) The Effects of Freezing at Low pH on the Biochemical Properties of Choline Oxidase, Collegiate Environmental Science & Health Symposium, Atlanta, GA, U.S.A.

10. Belaineh, M. G., and Gadda, G. (2006) The Catalytic Role of the Conserved Residue His196 of 2-Nitropropane Dioxygenase from Neurospora crassa, 12th Annual SAEOPP/UTK McNair National Scholars Research Conference, Knoxville, Tennessee, U.S.A.

11. Finnegan, S., and Gadda, G. (2008) Enzyme Catalysis in Slow-Motion - Story about a Mutant Flavoenzyme, Molecular Basis of Disease Day, Atlanta, GA, U.S.A.

12. Francis, K., and Gadda, G. (2009) The Single Electron Transfer Mechanism of N. crassa 2-Nitropropane Dioxygenase, University of Edinburgh, Edinburgh, Scotland

13. Francis, K., and Gadda, G. (2009) Branching of an Intermediate Formed during Turnover of N. crassa 2-Nitropropane Dioxygenase Inflates the Observed Kinetic Isotope Effect for the Reaction, Isotopes 2009, Cluj-Napoca, Romania


14. Yuan, H., and Gadda, G. (2011) Rapid Kinetic Investigations of D-Arginine Dehydrogenase and Active Site Mutants of Choline Oxidase Using Kinetic Isotope Effects, Albert Einstein School of Medicine, New York, NY, U.S.A.


16. Gannavaram, S., and Gadda. G. (2011) D-Amino Acid Processing by Flavoproteins, Molecular Basis of Disease Fellows Meet, Atlanta, GA, U.S.A.


18. Gannavaram, S., and Gadda, G. (2013) “How an active-site mutation tweaks the identity of cofactor in D-arginine dehydrogenase”, Molecular Basis of Disease Area of Focus Research Day, Atlanta, GA, U.S.A.

19. Smitherman, C., Rungsrisuriyachai, K. Germann, M.W., and Gadda, G. (2013) Reevaluation of the role of H466 in the active site of choline oxidase, 65th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

20. Su, D., Francis, K., and Gadda, G. (2013) Characterization of bacterial propionate 3-nitronate monooxygenase from Pseudomonas sp. Strain JS189, 65th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

21. Ouedraogo, D., and Gadda, G. (2013) Mechanistic study of D-arginine dehydrogenase I335H with pH profiles and rapid kinetics, 65th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

22. Gannavaram, S., Spring, A.M., Germann, M.W., and Gadda, G. (2013) Solving the puzzle of a green flavin isolated from D-arginine dehydrogenase, 65th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

23. Salvi, F., and Gadda., G. (2014) Seeking the Real Hero: Structural/Functional Prediction for Propionate 3-Nitronate Detoxification, Molecular Basis of Disease Area of Focus Research Day, Atlanta, GA, U.S.A.

24. Salvi, F., and Gadda., G. (2014) Improving Gene Function Prediction of Nitronate Monooxygenases with Structural/Functional Studies, EMBL, Heidelberg, Germany.

25. Smitherman, C., and Gadda, G. (2015) Class I Nitronate Monooxygenases: a New Family of Detoxifying Enzymes, 24th Enzyme Mechanisms Conference, Galveston, TX.

26. Smitherman, C. (2015) “Cleaning House: Detoxification of a Suicide Inhibitor by a Nitronate Monooxygenase.” Berry College. Mount Berry, GA, U.S.A.

27. Smitherman, C. (2015) “Excited State Proton Transfers in Flavoprotein Oxidases.” Gordon Research Seminar on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.


28. Aguillon, C. and Gadda, G. (2015) “Probing the Role of Tyrosine 254 in Class I Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” Molecular Basis of Disease Undergraduate Fellowship Presentation, Atlanta, GA, U.S.A.

29. Aguillon, C. and Gadda, G. (2016) “Probing the Role of Tyrosine 254 in Class I Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” Southeast Undergraduate Research Conference, Atlanta, GA, U.S.A.

30. Ball, J. (2016) “Spectroscopic and Kinetic Characterization of an FMN-dependent Quinone Reductase.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

31. Flores, E. (2016) “Characterization of Recombinant PA1225 for Validation of a Putative NAD(P)H-Quinone Oxidoreductase in Pseudomonas aeruginosa Strain PAO1.” 2016 Chemistry Research Symposium, Atlanta, GA, U.S.A.

32. Aguillon, C. (2016) “Kinetic and Spectroscopic Variants from Pseudomonas aeruginosa” Molecular Basis of Disease and NSF-REU Summer Undergraduate Research Luncheon, Atlanta, GA, U.S.A.

33. Harrell, C. (2016) “Studies of Y249F Mutant of PaDADH.” STEP 2016 Symposium, Clarkston, GA, U.S.A.

34. Su, D. (2017) “Studies on Nitronate Monooxygenase Mechanism and Fluorophore” Molecular Basis of Disease Fellows Talk, Atlanta, GA, U.S.A.

d.vi) Students Conference Abstracts (total: 181)

1. Johnson, K.L., Kanazawa, J.T., McAllister, E., Russell, B., and Gadda G. (2001) Structure-Function Studies of Flavin-Dependent Enzymes, 9th Annual F.L. (Bud) Suddath Memorial Symposium “Structural Biology”, Atlanta, GA, U.S.A.

2. Russell, B., and Gadda, G. (2001) Expression in Escherichia coli of Choline Dehydrogenase From Sinorhizobium meliloti, 53rd Southeast Regional Meeting of the American Chemical Society, Savannah, GA, U.S.A

3. Russell, B., and Gadda, G. (2001) Expression in Escherichia coli of Choline Dehydrogenase From Sinorhizobium meliloti, Nations Symposium, Atlanta, GA, U.S.A.

4. Johnson, K.L., and Gadda, G. (2002) Purification and Characterization of FMN-Containing 2-Nitropropane Dioxygenase from Neurospora crassa, 10th Annual F.L. (Bud) Suddath Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, GA, U.S.A.

5. Fan F., and Gadda, G. (2002) Cloning of codA Gene Coding for Choline Oxidase from Arthrobacter globiformis, 10th Annual F.L. (Bud) Suddath Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, GA, U.S.A.

6. Seyfe, D., and Gadda, G. (2002) Expression, Purification, and Enzymatic Activity of FAD-Containing 2-Nitropropane Dioxygenase from Hansenula mrakii, 10th Annual F.L. (Bud) Suddath Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, GA, U.S.A.


7. Russell, B., and Gadda, G. (2002) Expression in Escherichia coli and Refolding of Choline Dehydrogenase from Sinorhizobium meliloti, 10th Annual F.L. (Bud) Suddath Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, GA, U.S.A.

8. Wilkins, L., and Gadda, G. (2002) Cloning, Expression, and Preliminary Studies of Choline Dehydrogenase from Halomonas elongata, 10th Annual F.L. (Bud) Suddath Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, GA, U.S.A.

9. Powell, N., and Gadda, G. (2002) Cloning, Expression, and Preliminary Characterization Studies of Choline Dehydrogenase from Escherichia coli, 10th Annual F.L. (Bud) Suddath Memorial Symposium “A Discussion by Experimentalists of The Origin of Life”, Atlanta, GA, U.S.A.

10. Fan, F., Powell, N., Wilkins, L., and Gadda, G. (2002) Comparative Study of Choline Oxidizing Enzymes From Escherichia coli, Halomonas elongata, and Arthrobacter globiformis, Fourteenth International Symposium on Flavins and Flavoproteins, Cambridge, UK

11. Johnson, K.L., Russell, B., Seyfe, D., and Gadda, G. (2002) Purification and Characterization of Recombinant 2-Nitropropane Dioxygenase From Neurospora crassa and Hansenula mrakii, Fourteenth International Symposium on Flavins and Flavoproteins, Cambridge, UK

12. Russell, B., and Gadda, G. (2003) Steady State Kinetic Studies of Neurospora crassa 2-Nitropropane Dioxygenase, 18th Enzyme Mechanisms Conference, Galveston, TX, U.S.A.

13. Francis K., and Gadda G. (2003) Kinetic and Spectroscopic Studies on the Oxidized Form of Choline Oxidase, 11th Annual F.L. (Bud) Suddath Memorial Symposium “Cancer Research in Georgia”, Atlanta, GA, U.S.A.

14. Ghanem M., and Gadda G. (2003) Biophysical and Kinetic Characterization of Choline Oxidase from Arthrobacter globiformis, 11th Annual F.L. (Bud) Suddath Memorial Symposium “Cancer Research in Georgia”, Atlanta, GA, U.S.A.

15. Powell, N., Ghanem, M., Francis, K., Fan F., and Gadda G. (2003) Active Site Characterization of Recombinant Choline Oxidase, 29th Steenbock Symposium “Coenzymes, Cofactors and Catalysis, Madison”, WI, U.S.A.

16. Fan F., and Gadda G. (2003) pH and Kinetic Isotope Effect Studies on Recombinant Choline Oxidase from Arthrobacter globiformis, 29th Steenbock Symposium “Coenzymes, Cofactors and Catalysis, Madison”, WI, U.S.A.

17. Menon, P., Fan, F., Ghanem, M., Francis, K., Powell, N., Nguyen, T., Tolbert, A., and Gadda, G. (2003) Biochemical and Kinetic Characterization of Choline Oxidase from Arthrobacter globiformis, 55th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.


18. Fan, F., and Gadda, G. (2005) On the Mechanism of Alcohol Oxidation Catalyzed by Choline Oxidase, 19th Enzyme Mechanisms Conference, Asilomar, CA, U.S.A.

19. Zheng, J., Ghanem, M., Gadda, G., and Shamshi, S.A. (2005) Simultaneous, Quantitative Analysis of Choline, Betaine Aldehyde, and Glycine Betaine Using Capillary Zone Electrophoresis-Electrospray Mass Spectrometry (CZE-ESI-MS): Kinetic Studies of Choline Oxidases, Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Orlando, FL, U.S.A.

20. Francis, K., and Gadda, G. (2006) The Catalytic Mechanism of 2-Nitropropane Dioxygenase Involves the Formation of an Anionic Semiquinone, 3rd Annual SER-CAT Symposium, Atlanta, GA, U.S.A.

21. Quaye, O. and Gadda, G. (2006) Mechanistic Investigation of the Glu312Asp Variant of Choline Oxidase, 3rd Annual SER-CAT Symposium, Atlanta, GA, U.S.A.

22. Rungsrisuriyachai, K., and Gadda, G. (2006) Investigation of the Active Site Histidine 351 of Choline Oxidase, 3rd Annual SER-CAT Symposium, Atlanta, GA, U.S.A.

23. Hoang, J., V., and Gadda, G. (2006) The Hysteretic Behavior of Choline Oxidase, 3rd Annual SER-CAT Symposium, Atlanta, GA, USA, U.S.A.

24. Belaineh, M., and Gadda, G. (2006) Role of the Conserved Residue His196 of 2-Nitropropane Dioxygenase, 3rd Annual SER-CAT Symposium, Atlanta, GA, U.S.A.

25. Francis, K., and Gadda, G. (2006) An Anionic Flavosemiquinone is Involved in the Enzymatic Oxidation of Nitroalkanes Catalyzed by 2-Nitropropane Dioxygenase, 2nd Annual National Symposium on Prostate Cancer, Atlanta, GA, U.S.A.

26. Ghanem, M., and Gadda, G. (2006) Effects of the Protein Charge Close to the N(1)-Flavin Locus on Choline Oxidase, 2nd Annual National Symposium on Prostate Cancer, Atlanta, GA, U.S.A.

27. Hoang, J.V., and Gadda, G. (2006) Reversible Inactivation of Choline Oxidase by Freezing at Low pH, 2nd Annual National Symposium on Prostate Cancer, Atlanta, GA, U.S.A.

28. Francis, K., and Gadda, G. (2006) The Oxidation of Nitroalkanes by 2-Nitropropane Dioxygenase: Involvement of a Flavosemiquinone in Catalysis, 231st American Chemical Society National Meeting, Atlanta, GA, U.S.A.

29. Ghanem, M., and Gadda, G. (2006) On the Effects of Reversing the Protein Positive Charge in Proximity of the N(1)-Flavin Locus in Choline Oxidase, Experimental Biology 2006, San Francisco, California, U.S.A.

30. Hoang, T. V., and Gadda, G. (2006) The Effects of Freezing at Low pH on the Biochemical Properties of Choline Oxidase, Collegiate Environmental Science & Health Symposium, Atlanta, GA, U.S.A.

31. Finnegan, S., Hoang, J.V., and Gadda, G. (2007) Importance of Val464 in Catalysis of Choline Oxidase, 20th Enzyme Mechanisms Conference, St. Pete Beach, FL, U.S.A.


32. Francis, K., Belaineh, M., and Gadda, G. (2007) Reductive Half Reaction of 2-Nitropropane Dioxygenase: Involvement of an Electrostatic Catalyst in the Formation of an Anionic Flavosemiquinone, St. Pete Beach, FL, U.S.A.

33. Mijatovic, S., Nguyen, T., and Gadda, G. (2007) Reevaluation of the Prosthetic Group and Substrate Specificity of 2-Nitropropane Dioxygenase from Hansenula mrakii, St. Pete Beach, FL, U.S.A.

34. Quaye, O., and Gadda, G. (2007) On the Role of Glu312 in the Binding and Correct Positioning of the Substrate for Efficient Catalysis in Choline Oxidase, St. Pete Beach, FL, U.S.A.

35. Rungsrisuriyachai, K., and Gadda, G. (2007) On the Role of Active Site Histidine 351 in the Reaction Catalyzed by Choline Oxidase, St. Pete Beach, FL, U.S.A.

36. Quaye, O., and Gadda, G. (2007) Role of Glu312 in the Catalytic Mechanism of Choline Oxidase, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, GA, U.S.A.

37. Francis, K., and Gadda, G. (2007) Role of the Anionic Flavosemiquinone in the Reductive Half-Reaction of 2-Nitropropane Dioxygenase, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, GA, U.S.A.

38. Yuan, H., and Gadda, G. (2007) Kinetic Analysis of Ser101 Active Site Variants of Choline Oxidase, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, GA, U.S.A.

39. Rungsrisuriyachai, K., and Gadda, G. (2007) Role of His351 in the Active Site of Choline Oxidase, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, GA, U.S.A.

40. Mijatovic, S., and Gadda, G. (2007) Biophysical and Biochemical Characterization of 2-Nitropropane Dioxygenase from Hansenula mrakii, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, GA, U.S.A.

41. Nguyen, T., and Gadda, G. (2007) Rescuing the Activity of Choline Oxidase E312D Mutant Using a Substrate Analogue, 15th Annual Bud Suddath Bioscience Symposium "Directed Molecular Evolution Mechanisms", Atlanta, GA, U.S.A.

42. Gadda, G., Francis, K., and Belaineh, M. (2007) The Mechanism of 2-Nitropropane Dioxygenase: a Model for Flavin Semiquinone Intermediates in Enzymatic Catalysis, Experimental Biology 2007, Washington, D.C., U.S.A.

43. Finnegan, S., and Gadda, G. (2007) Effect of Removing a Hydrophobic Residue from the Active Site on Choline Oxidase, Molecular Basis of Disease Day, Atlanta, GA, U.S.A.

44. Gadda, G., Rungsrisuriyachai, K., Quaye, O., Finnegan, S., Yuan, H., Nguyen, T., and Orville, A.M. (2007) Preorganization of the Enzyme-Substrate Complex in the Reaction of Alcohol Oxidation Catalyzed by Choline Oxidase, Gordon Research Conference of Enzymes, Coenzymes, and Metabolic Pathways, University of New England, Biddeford, ME, U.S.A.


45. Francis, K., and Gadda, G. (2007) Probing the Nonoxidative Tautomerization of Nitroethane to Ethylnitronate Catalyzed by 2-NItropropane Dioxygenase with pH and Substrate Kinetic Isotope Effects. 234th ACS National Meeting, Boston, MA, U.S.A.

46. Francis, K., and Gadda, G. Partition Isotope Effects for Enzymatic Reactions Occurring Through Multiple Pathways: The Observed Kinetic Isotope Effect in the 2-Nitropropane Dioxygenase Reaction, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Ventura, CA, U.S.A.

47. Orville, A.M., Prabhakar, R., Lountous, G.T., Finnegan, S., and Gadda, G. (2008) A Flavin-C4a-Oxygen Intermediate Observed by Crystallographic, Spectroscopic and Computational Methods, Sixteenth International Symposium on Flavins and Flavoproteins, Jaca, Spain

48. Gadda, G., Pennati, A., Francis, K., Quaye, O., Yuan, H., Rungsrisuriyachai, K., Finnegan, S., Mijatovic, S., Nguyen, T., and Orville, A.M. (2008) Hydride Transfer Made Easy in the Oxidation of Alcohols Catalyzed by Choline Oxidase, Sixteenth International Symposium on Flavins and Flavoproteins, Jaca, Spain

49. Finnegan, S., and Gadda, G. (2008) Hydrophobic Packing effects on the Reductive Half-reaction of Choline Oxidase, Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.

50. Quaye, O., Cowins, S., and Gadda, G. (2008) Contribution of FAD C*M Covalent Linkage with His99 to the Reaction Catalyzed by Choline Oxidase, Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.

51. Law, L., Rungsrisuriyachai, K., and Gadda, G. (2008) Preliminary Investigation of the Role of the Conserved, Active Site Asp510 in the Reaction Catalyzed by Choline Oxidase (2008) Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.

52. Rungsrisuriyachai, K., and Gadda, G. (2008) pH Effects on the Steady State Kinetic Mechanism of Pyranose 2-Oxidase from Trametes ochracea, Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.

53. Yuan, H., Sherman, S., and Gadda, G. (2008) Catalytic Importance of the Hydrophilic Side-chain with H-bonding Capability of Ser101 at the Active Site of Choline Oxidase, Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.

54. Francis, K., Chapman, N., and Gadda, G. (2008) Mutation of Histidine 196 to Asparagine in N. crassa 2-Nitropropane Dioxygenase Improves the Catalytic Efficiency of the Enzyme with Anionic Substrates, Second International Interdisciplinary Conference on Vitamins, Cofactors, and Biofactors, Athens, GA, U.S.A.

55. Finnegan, S., Yuan, H. and Gadda, G. (2008) Novel insights on the reactivity of reduced flavoenzymes with oxygen, 235th National Meeting of the American Chemical Society, New Orleans, LA, U.S.A.


56. Finnegan, S., Yuan, H., Hoang, J.V. and Gadda, G. (2008) A Proton Driven Switch that Activate/Inactivate Choline Oxidase, Gordon Research Conference on Enzymes, Coenzymes & Metabolic Pathways, University of New England, Biddeford, ME, U.S.A.

57. Finnegan, S., and Gadda, G. (2008) Substitution of an Active Site Valine Uncovers a Kinetically Slow Equilibrium between Active and Inactive Forms of Choline Oxidase, Biotech symposium 2008, Georgia State University, Atlanta, GA, U.S.A.

58. Finnegan, S., and Gadda, G. (2008) Hydrophobic Packing effects on the Reductive Half-reaction of Choline Oxidase, Second International Interdisciplinary Conference on Vitamins, Coenzymes, and Biofactors, Athens, GA, U.S.A.

59. Yuan, H., and Gadda, G. (2009) The Importance of Serine Serine 101 at the Active Site of Choline Oxidase for Hydroxyl Proton Abstraction, 7th Annual Biotechnology Symposium, Atlanta, GA, U.S.A.

60. Yuan, H., and Gadda, G. (2009) Catalytic Importance of the Hydrophilic Side-chain of Ser101 at the Active Site of Choline Oxidase, the 75th Herty Medal Symposium, Georgia Institute of Technology, Atlanta, GA, U.S.A.

61. Yuan, H., and Gadda, G. (2009) Catalytic Importance of the Hydrophilic Side-chain with H-bonding Capability of Ser101 at the Active Site of Choline Oxidase, Florida Annual Meeting and Exposition 2009, Orlando, FL, U.S.A.

62. Rungsrisuriyachai, K. and Gadda, G. (2009) pH Effect on the Steady State Kinetic Mechanism of Pyranose 2- Oxidase from Trametes ochracea, the 75th Herty Medal Symposium, Georgia Institute of Technology, Atlanta, GA, U.S.A.

63. Rungsrisuriyachai, K. and Gadda, G. (2009) Role of Asn510 in the Relative Timing of Substrate Bond Cleavages in the Reaction Catalyzed by Choline Oxidase, 7th Annual Biotechnology Symposium, Georgia State University, Atlanta, GA, U.S.A.

64. Francis, K., and Gadda, G. (2009) Kinetic Evidence for Nitro Binding Pocket in the Active Site of 2-Nitropropane Dioxygenase, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

65. Francis, K., and Gadda, G. (2009) Branching of an Intermediate Formed during Turnover of N. crassa 2-Nitropropane Dioxygenase Inflates the Observed KIE for the Reaction, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

66. Francis, K., and Gadda, G. (2009) The Observed KIE for the Reaction of 2-Nitropropane Dioxygenase is Inflated Due the Branching of a Reaction Intermediate Formed During Turnover, 75th Herty Medal Symposium, Atlanta, GA, U.S.A.

67. Francis, K., and Gadda, G. (2009) Kinetic Evidence for Nitro Binding Pocket in the Active Site of Nitronate Monooxygenase, 7th Annual Biotechnology Symposium at Georgia State University, Atlanta, GA, U.S.A.

68. Finnegan, S., and Gadda, G. (2009) Choline Oxidase – Overcoming the Spin-forbidden Reaction with Oxygen, 75th Herty Medal Symposium, Atlanta, GA, U.S.A.


69. Finnegan, S., and Gadda, G. (2009) Role of Val464 in the Flavin Oxidation Reaction Catalyzed by Choline Oxidase, 7th Annual Biotechnology Symposium at Georgia State University, Atlanta, GA, U.S.A.

70. Pennati, A. and Gadda, G. (2009) The Effect of pH on the Reductive and Oxidative Half-Reactions Catalyzed by FMN-dependent Human Glycolate Oxidase, 75th Herty Medal Symposium, Atlanta, GA, U.S.A.

71. Pennati, A., and Gadda, G. (2009) pH Effects on the Reaction of Glycolate Oxidation Catalyzed by Human Liver Glycolate Oxidase, 7th Annual Biotechnology Symposium at Georgia State University, Atlanta, GA, U.S.A.

72. Law, L., and Gadda, G. (2009) Engineering a Soluble Variant of Choline Dehydrogenase from Halamonas Elongata, 21st Enzyme Mechanisms Conference, Tucson, AZ, U.S.A.

73. Law, L., and Gadda, G. (2009) Purification of Recombinant Choline Dehydrogenase from Halamonas Elongata, Florida Annual Meeting and Exposition 2009, Orlando, FL, U.S.A.

74. Law, L., and Gadda, G. (2009) Purification of Recombinant Choline Dehydrogenase from Halamonas Elongata, the 75th Herty Medal Symposium, Georgia Institute of Technology, Atlanta, GA, U.S.A.

75. Law, L., and Gadda, G. (2009) Purification of Recombinant Choline Dehydrogenase from Halamonas Elongata, 7th Annual Biotechnology Symposium, Atlanta, GA, U.S.A.

76. Sherman, S., Yuan, H., and Gadda, G. (2009) Importance of the Hydrophilic Side-chain of Serine 101 in the Active Site of Choline Oxidase for Catalysis, 3rd Annual Georgia State Undergraduate Research Conference, Atlanta, GA, U.S.A.

77. Sherman, S., Pennati, A., and Gadda, G. (2009) Purification and Preliminary Characterization of Choline Oxidase from Aspergillus fumigates, 7th Annual Biotechnology Symposium at Georgia State University, Atlanta, GA, U.S.A.

78. Rungsrisuriyachai, K., and Gadda, G. (2010) Role of Asn 510 in the Relative Timing of Substrate Bond Cleavages in the Reaction Catalyzed by Choline Oxidase, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Galveston, TX, U.S.A.

79. Francis, K, and Gadda, G. (2010) Temperature and Kinetic Isotope Effects on the Nitronate Monooxygenase Catalyzed reaction, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Galveston, TX, U.S.A.

80. Finnegan, S., and Gadda, G. (2010) The Nature of the Kinetically Slow Equilibrium Between Two Conformers of the Val464Ala Variant of Choline Oxidase, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.

81. Francis, K., and Gadda, G. (2010) A Novel Function of Nitronate Monooxygenase: Detoxification of the Plant Toxin Propionate-3-Nitronate, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.


82. Gannavaram, S., and Gadda, G. (2010) Kinetic Isotope Effect of Choline Oxidase Wild Type from Arthrobacter globiformis, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.

83. Law, L., and Gadda, G. (2010) Investigation of D-Arginine Dehydrogenase Tyr249 Mutants, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.

84. Pennati, A., and Gadda, G. (2010) Stabilization of a Flavosemiquinone in the Oxidative Half Reaction of Human Liver Glycolate Oxidase, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.

85. Rungsrisuriyachai, K., and Gadda, G. (2010) Role of Asparagine 510 in the Relative Timing of Substrate Bind Cleavages in the Reaction Catalyzed by Choline Oxidase, First Southeast Enzyme Conference, Atlanta, GA, U.S.A.

86. Francis, K., and Gadda, G. (2010) A Novel Function of Nitronate Monooxygenase: Detoxification of the Plant Toxin Propionate-3-Nitronate, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

87. Smitherman, C., Rungsrisuriyachai, K., and Gadda, G. (2011) Is His466 the Catalytic Base for Choline Oxidase from Arthrobacter globiformis?, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

88. Uluisik, R., Gannavaram , S., and Gadda, G. (2011) Effect of Temperature on the Kinetic Isotope Effects of the Active Site Variant S101A of Choline Oxidase, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

89. Gannavaram, S., Law, L., Yuan, H., Fu, G., Weber, I., and Gadda, G. (2011) Role of Tyrosine 249 in substrate binding of D-Arginine Dehydrogenase from Pseudomonas aeruginosa, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

90. Francis, K., Nishino, S., Spain, J. and Gadda, G. (2011) A Novel Activity for Fungal Nitronate Monooxygenase: Detoxification of the Metabolic Inhibitor Propionate-3-Nitronate, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

91. Pennati, A., Lambou, K., J-P., Latge, and Gadda, G. (2011) Choline-Glycine Betaine Metabolic Pathway in Aspergillus fumigatus, Southeast Enzyme Conference, Atlanta, GA U.S.A.

92. Yuan, H., and Gadda, G. (2011) On the Catalytic Mechanism of D-Arginine Dehydrogenase, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

93. Smitherman, C., Francis, K., Nishino, S., Spain, J., and Gadda, G. (2011) Detoxification of a Lethal Toxin by Nitronate Monooxygenase, Seventeenth International Symposium on Flavins and Flavoproteins, Berkeley, CA, U.S.A.

94. Gannavaram, S., and Gadda. G. (2011) Role of Tyrosine249 in Substrate Binding of D-Arginine Dehydrogenase from Pseudomonas aeruginosa, Second Southeast Enzyme Conference, Atlanta, GA, U.S.A.



96. Uluisik, R., and Gadda. G. (2011) Effect of Temperature on the Kinetic Isotope Effects of the Active Site Variant S101A of Choline Oxidase, Second Southeast Enzyme Conference, Atlanta, GA, U.S.A.

97. Smitherman, C., and Gadda. G. (2011) Is a Catalytic Base Required for Choline Activation in Choline Oxidase from Arthrobacter globiformis, Second Southeast Enzyme Conference, Atlanta, GA, U.S.A.

98. Smitherman, C., and Gadda, G. (2012) Secondary Kinetic Isotope Effects as Probes to Investigate Catalysis in Nitronate Monooxygenase, Gordon Research Conference on Isotopes in the Biological and Chemical Sciences, Galveston, TX, U.S.A.

99. Uluisik, R., and Gadda, G. (2012) Importance of a Serine Proximal to the C(4) and N(5) Flavin Atoms for Quantum Mechanical Tunneling of the Hydride in Choline Oxidase, Gordon Research Conference on Isotopes in the Biological and Chemical Sciences, Galveston, TX, U.S.A.

100. Salvi, F., and Gadda, G. (2012) Importance of the Hydrophobic Cluster Controlling Substrate Access for the Hydride Transfer Reaction Catalyzed by Choline Oxidase. Gordon Research Conference on Isotopes in the Biological and Chemical Sciences, Galveston, TX, U.S.A.


102. Smitherman, C., and Gadda. G. (2012) On the Catalytic Mechanism of Nitronate Monooxygenase, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

103. Gannavaram, S., and Gadda. G. (2012) Relative Timing of Proton Transfers in the Reduction of Oxygen in Choline Oxidase from Arthrobacter globiformis, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

104. Uluisik, R., and Gadda. G. (2012) The role of S101 in Quantum Mechanical Hydride Tunneling in Choline Oxidase, Southeast Enzyme Conference, Atlanta, GA, U.S.A.

105. Salvi, F., and Gadda. G. (2012) Role of the Hydrophobic Cluster in the Hydride Transfer Reaction Catalyzed by Choline Oxidase, Atlanta, GA, U.S.A.

106. Francis, K., Nishino, S., Smitherman, C., Spain, J., and Gadda. G. (2012) Detoxification of the Metabolic Toxin Propionate-3-Nitronate by Fungal Nitronate Monooxygenase, The Florida Annual Meeting and Exposition, Tampa, FL, U.S.A.

107. Gannavaram, S., and Gadda, G. (2012) Relative Timing of Proton Transfers in Oxygen Reduction Catalyzed by Choline Oxidase, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

108. Santos, Y.J., Smitherman, C., and Gadda, G. (2012) Purification of a Detoxifying Enzyme from Williopsis saturnus, Herty Medalist Undergraduate Research Symposium, Atlanta, GA, U.S.A.

109. Bui, Q.V., Gebrehiwot, E.B., Gannavaram, S., and Gadda, G. (2012) Comparison of Kinetic Parameters of D-Arginine Dehydrogenase Using Different Substrates as a Function of pH, Herty Medalist Undergraduate Research Symposium, Atlanta, GA, U.S.A.


110. Ball, J., Gannavaram, S., and Gadda, G. (2012) pH Effects on the Steady-State Turnover of D-Arginine Dehydrogenase with D-Arginine as Substrate, Herty Medalist Undergraduate Research Symposium, Atlanta, GA, U.S.A.

111. Gannavaram, S., and Gadda, G. (2013) Mechanism of Flavin oxidation in Choline Oxidase, 23rd Enzyme Mechanisms Conference, Coronado Bay, CA, U.S.A.

112. Bui, Q., Gannavaram, S., and Gadda, G. (2013) pH and Solvent Viscosity Effects of D-Arginine Dehydrogenase with Different D-Amino Acid Substrates, Georgia State University Undergraduate Research Conference, Atlanta, GA, U.S.A.

113. Ball, J., Gannavaram, S., and Gadda, G. (2013) Investigation of Ionizable Groups that Participate in D-Arginine Dehydrogenase Catalysis and Binding, Georgia State University Undergraduate Research Conference, Atlanta, GA, U.S.A.

114. Smitherman, C., and Gadda, G. (2013) An Ongoing Journey to Crystallize a Detoxifying Enzyme, Nitronate Monooxygenase from Williopsis saturnus, Fourth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

115. Santos, Y.J., Smitherman, C., and Gadda, G. (2013) Investigation of Substrate Oxidation in Choline Oxidase through Further Mutagenesis of Histidine Residues., Fourth Southeast Enzyme Conference, Atlanta, GA, U.S.A.


117. Bui, Q., Gannavaram, S., and Gadda, G. (2013) pH Profiles of D-Arginine Dehydrogenase with D-Histidine and D-Methionine, Fourth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

118. Ball, J., Gannavaram, S., and Gadda, G. (2013) Kinetic Analysis of D-Arginine Dehydrogenase by pH and Inhibition Effects, Fourth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

119. Ouedraogo, D., Salvi, F. and Gadda, G. (2013) Mutagenesis, Expression, Purification, and Characterization of the D-Arginine Dehydrogenase Variant, I335H, Fourth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

120. Romero, E., and Gadda, G. (2013) Effect of temperature on the reductive-half reaction of human glycolate oxidase, Fourth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.


122. Gannavaram, S., and Gadda, G. (2013) Characterization of a Green Flavin from D-Arginine Dehydrogenase. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.


123. Romero, E., and Gadda, G. (2013) Temperature and Solvent Viscosity Dependence of the Reductive-half Reaction of Human Glycolate Oxidase. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

124. Smitherman, C., Rungsrisuriyachai, K., Germann, M.W., and Gadda, G. (2013) Mechanistic Studies of Choline Oxidase H466Q. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

125. Romero, E., and Gadda, G. (2013) Evidence for hydride tunneling in the human glycolate oxidase reaction: A stopped-flow study at various temperatures, 65th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

126. Ball, J., Su, D., and Gadda, G. (2013) Chemical modification and site-directed mutagenesis of His48 in D-Arginine Dehydrogenase, 65th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

127. Salvi, F., Wang, Y.F., Weber, I.T., and Gadda, G. (2013) Crystal structure of choline oxidase from Arthrobacter globiformis in complex with glycine betaine suggests the importance of the dimer interface for substrate access to the active site, 65th Southeast Regional Meeting of the American Chemical Society, Atlanta, GA, U.S.A.

128. Romero, E., and Gadda, G. (2014) Kinetic Isotope Effects in Human Glycolate Oxidase: Evidences for Hydride Tunneling. Gordon Research Conference on Isotopes in the Biological and Chemical Sciences, Galveston, TX, U.S.A.

129. Hernandez, J., Smitherman, C., Salvi F., and Gadda, G. (2014) Improved Recombinant Expression of Bacterial Nitronate Monooxygenase in Escherichia coli. 6th Annual Poster Day, Atlanta, GA, U.S.A.

130. Ouedraogo, D. and Gadda, G. (2014) Perturbation of pKa Values Enabling the Determination of the Kinetic Rate Constants in the Reductive Half-reaction of D-Arginine Dehydrogenase Variant, I335H. 6th Annual Poster Day, Atlanta, GA, U.S.A.

131. Su, D., Francis, K., and Gadda, G. (2014) Characterization of bacterial propionate 3-nitronate monooxygenase from Pseudomonas sp. strain JS189. 6th Annual Poster Day, Atlanta, GA, U.S.A.

132. Ball, J. and Bui, Q., Gannavaram, S., and Gadda, G. (2014) pH and kinetic isotope effects of ᴅ-arginine dehydrogenase with multiple substrates. 6th Annual Poster Day, Atlanta, GA, U.S.A.

133. Gannavaram, S. and Gadda, G. (2014) Characterization of a green flavin from ᴅ-arginine dehydrogenase. 6th Annual Poster Day, Atlanta, GA, U.S.A.

134. Salvi F., Wang Y.F., Weber I.T., and Gadda G., (2014) Crystal structure of choline oxidase from Arthrobacter globiformis in complex with glycine betaine suggests the importance of the dimer interface for substrate access to the active site. 6th Annual Poster Day, Atlanta, GA, U.S.A.

135. Smitherman, C., Rungsrisuriyachai, K., Germann, M.W., Gadda, G. (2014) Mechanistic studies of choline oxidase H466Q. 6th Annual Poster Day, Atlanta, GA, U.S.A.


136. Romero, E. and Gadda, G. (2014) Hydride transfer in human glycolate oxidase takes place by tunneling. 6th Annual Poster Day, Atlanta, GA, U.S.A.

137. Bui, Q., Salvi, F., and Gadda, G. (2014) Steady-state kinetics of nitronate monoxygenase from Pseudomonas aeruginosa (PA4202), Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

138. Romero, E., and Gadda, G. (2014) Involvement of hydride tunneling and dynamics in the reductive half-reaction of human glycolate oxidase, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

139. Salvi, F., Agniswamy, J., Yuan, H., Vercammen, K., Pelicaen, R., Cornelis, P., Spain, J., Weber, I.T. and Gadda, G. (2014) The combined structural and kinetic characterization of a bacterial nitronate monooxygenase from Pseudomonas aeruginosa PAO1 establishes a class of enzymes, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

140. Ball, J., Gannavaram, S., and Gadda, G. (2014) pH dependence, chemical modification, and site-directed mutagenesis of PaDADH: Implications for a catalytic base, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

141. Su, D., Francis, K., and Gadda, G. (2014) Investigation on the catalytic mechanism of flavin-dependent propionate-3-nitronate monooxygenase from Pseudomonas sp. Strain JS189, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

142. Gannavaram, G., Sirin, S., and Gadda, G. (2014) Mechanistic and computational studies on C-N bond oxidation catalyzed by D-arginine dehydrogenase, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

143. Ouedraogo, D., and Gadda, G. (2014) Mechanistic investigation of D-arginine dehydrogenase variant, I335H, Fifth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

144. Hernandez, J., Smitherman, C., Salvi, F., and Gadda, G. (2014) Strategies for enhancing recombinant expression of bacterial nitronate monooxygenase, Georgia-Alabama Louis Stokes Alliances for Minority Participation (LSAMP) Spring Symposium, Atlanta, GA, U.S.A.

145. Hernandez, J., Smitherman, C., Salvi, F., and Gadda, G. (2014) Optimizing the expression of nitronate monooxygenase in Escherichia coli, Georgia State Undergraduate Research Conference, Atlanta, GA, U.S.A.

146. Hernandez, J., Smitherman, C., Salvi, F., and Gadda, G. (2014) Effective induction conditions for the recombinant expression of bacterial nitronate monooxygenase, Fifth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

147. Smitherman, C., and Gadda, G. (2014) Effects of solvent viscosity on the reaction catalyzed by nitronate monooxygenase from Cyberlindnera saturnus, Fifth Southeast Enzyme Conference, Atlanta, GA, U.S.A.

148. Ouattara, M., Sachla, A.J., Romero, E., Agniswamy, J., Weber, I.T. and Gadda, G. (2014) Identification and characterization of HupZ: the first heme-degrading enzyme from Streptococcus sp, Fifth Southeast Enzyme Conference, Atlanta, GA, U.S.A.


149. Salvi, F., Agniswamy, J., Yuan, H., Vercammen, K., Pelicaen, R., Cornelis, P., Spain, J., Weber, I.T., and Gadda, G. (2014) The Combined Structural and Kinetic Characterization of a Bacterial Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1 Establishes a Class of Enzymes. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

150. Smitherman, C., and Gadda, G. (2014) Product Determination from the Enzymatic Detoxification of Propionate 3-Nitronate by Nitronate Monooxygenase. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, U.S.A.

151. Smitherman, C., Salvi, F., Su, D., Hudson, B., and Gadda, G. (2015) Class I Nitronate Monooxygenases: a New Family of Detoxifying Enzymes, 24th Enzyme Mechanisms Conference, Galveston, TX. Smitherman, C. and Gadda, G. (2015) “Excited State Proton Transfers in Flavoprotein Oxidases.” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

152. Smitherman, C. and Gadda, G. (2015) “Oxidative Detoxification of Propionate 3-nitronate by Nitronate Monooxygenase and Novel Detection of Enzymatic Products.” Sixth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

153. Ball, J., Salvi, F., and Gadda, G. (2015) “NADH:Quinone Reductase Activity of PA1024 from Pseudomonas aeruginosa .” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, U.S.A.

154. Ball, J., Salvi, F., and Gadda, G. (2015) “The Kinetic Characterization of PA1024 from Pseudomonas aeruginosa Establishes the Enzyme as an NADH:menadione Reductase and not a Nitronate Monooxygenase.” Sixth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

155. Su, D. and Gadda, G. (2015) “Mechanistic and Structural Study of Bacterial Propionate 3-nitronate from Pseudomonas sp. Strain JS189.” Sixth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.

156. Su, D. and Gadda, G. (2015) “Spectroscopic and Kinetic Study on Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

157. Ball, J., Salvi, F., and Gadda, G. (2015) “NADH:quinone Reductase Activity of PA1024 from Pseudomonas aeruginosa”, Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

158. Ouedraogo, D., Vasquez, S., and Gadda, G. (2015) “Kinetic and Biophysical Investigations of Residues Surrounding the Flavin in D-arginine Dehydrogenase from Pseudomonas aeruginosa” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

159. Ouedraogo, D., and Gadda, G. (2014) “Mechanistic Investigation of D-Arginine Dehydrogenase Variant, I335H” Sixth Annual Southeast Enzyme Conference, Atlanta, GA, U.S.A.


160. Aguillon, C. and Gadda, G. (2015) “Probing the Role of Tyrosine 254 in Class I Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1 by Site-Directed Mutagenesis.” Molecular Basis of Disease Undergraduate Fellowship Luncheon, Atlanta, Ga, U.S.A.

161. Flores, E., Su, D., and Gadda, G. (2016) “Characterization of a Putative NAD(P)H-Quinone Oxidoreductase in Pseudomonas aeruginosa Strain PAO1.” Southeast Undergraduate Research Conference, Atlanta, GA, U.S.A.

162. Iyer, A., Aguillon, C., Ball, J., Flores, E., Ouedraogo, D., Su, D., Tofighi, H., and Gadda, G. (2016) “GADDA Research Group.” Southeast Undergraduate Research Conference, Atlanta, GA, U.S.A.

163. Aguillon, C., Su, D., and Gadda, G. (2016) “Elucidating the Role of Select Residues through Site-Directed Mutagenesis in Class I Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

164. Aguillon, C., Su, D., and Gadda, G. (2016) “On the Role of Tyrosine 254 in Class I Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” GA-AL LSAMP 2016 Annual Research Scientists of Tomorrow Symposium, Atlanta, GA, U.S.A.

165. Ball, J., Salvi, F., and Gadda, G. (2016) “Spectroscopic and Kinetic Characterization of an FMN-dependent Quinone Reductase.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

166. Flores, E., Su, D., and Gadda, G. (2016) “Purification and Characterization of a Putative NAD(P)H-Quinone Oxidoreductase in Pseudomonas aeruginosa Strain PAO1.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

167. Flores, E., Su, D., and Gadda, G. (2016) “Characterization of Recombinant PA1225: a Hypothetical NAD(P)H-Quinone Oxidoreductase from Pseudomonas aeruginosa PAO1.” 10th Annual Georgia State University Undergraduate Research Conference, Atlanta, GA, U.S.A.

168. Flores, E., Su, D., and Gadda, G. (2016) “Purification and Characterization of a Putative NAD(P)H-Quinone Oxidoreductase in Pseudomonas aeruginosa Strain PAO1.” GA-AL LSAMP 2016 Annual Research Scientists of Tomorrow Symposium, Atlanta, GA, U.S.A.

169. Iyer, A., Gannavaram, S., Spring, A., Germann, M.W., and Gadda, G. (2016) “Characterization of the 6-Hydroxy-FAD of Y249F variant of D-Arginine Dehydrogenase from Pseudomonas aeruginosa.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

170. Ouedraogo, D., Vasquez, S., and Gadda, G. (2016) “Insights on the Role of Ser/Ala Switch in Amine Oxidation in Pseudomonas aeruginosa D-Arginine Dehydrogenase.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

171. Su, D. and Gadda, G. (2016) “Mechanistic and Spectroscopic Study of Bacterial Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.


172. Tofighi, H., Su, D., Aguillon, C., and Gadda, G. (2016) “Combined Mutagenesis and Kinetic Characterization of Bacterial Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” Seventh Southeast Enzyme Conference, Atlanta, GA, U.S.A.

173. Aguillon, C., Su, D., and Gadda, G. (2016) “Kinetic and Spectroscopic Variants from Pseudomonas aeruginosa.” Molecular Basis of Disease and NSF-REU Summer Undergraduate Research Luncheon, Atlanta, GA, U.S.A.

174. Ball,J., Salvi, F., Felix, T., and Gadda, G. (2016) “A New Class of NADH:quinone Reductases.” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

175. Felix, T., Ball, J., and Gadda, G. (2016) “Mutagenesis of a Predicted Catalytic Lysine in the Active Site of PA1024.” Molecular Basis of Disease and NSF-REU Summer Undergraduate Research Luncheon, Atlanta, GA, U.S.A.

176. Gadda, G., Su, D., Tofighi, H., and Kwok, M. (2016) “Direct Observation of Anionic and Neutral Flavosemiquinones during Catalysis of a Monooxygenase.” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

177. Kwok, M., Tofighi, H., Gadda, G. (2016) “Protein Expression and Purification of Nitronate Monooxygenase from Pseudomonas aeruginosa.” Molecular Basis of Disease and NSF-REU Summer Undergraduate Research Luncheon, Atlanta, GA, U.S.A.

178. Ouedraogo, D., Vasquez, S., Souffrant, M., Waiters, S., Marshall, S., Coleman, M., Adodo, E., Mariani, V., Hamelburg, D., and Gadda, G. (2016) “Kinetic and Biophysical Investigations of Ser/Ala Switch and Glu 246 in D-Arginine Dehydrogenase from Pseudomonas aeruginosa.” Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, Waterville Valley, NH, U.S.A.

179. Su. D. and Gadda, G. (2016) “Mechanistic Study of Bacterial Propionate 3-Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1.” Molecular Basis of Disease Graduate Research Day, Atlanta, GA, U.S.A.

180. Iyer, A., Reis, R., Gannavaram, S., Spring, A., Germann, M.W., Agniswamy, J., Weber, I.T., and Gadda., G. (2017) Structural Insights into the 6-OH-FAD in the Y249F Variant of D-Arginine Dehydrogenase from Pseudomonas aeruginosa, 25th Enzyme Mechanisms Conference, St. Pete Beach, FL, U.S.A.

181. Flores, E., and Gadda, G. (2017) Characterization of a New FAD:NAD(P)H-Quinone Oidoreductase in Pseudomonas aeruginosa Strain PAO1, 25th Enzyme Mechanisms Conference, St. Pete Beach, FL, U.S.A.

d.vii) Direction of Individual Student Work (Dissertations at GSU)

1. Nichole Powell, Ph.D. Dissertation: December 2003. Title: " Bacterial Choline-Oxidizing Systems: Characterization of Enzymes Involved in Stress Tolerance and The Roles of Cytochrome P450s 1A1, 1A2, and 2D6, and the GSTM1 Genes in Treatment Resistant Depression"; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.


2. Fan Fan, PhD Dissertation: December 2005. Title: "On the Catalytic Mechanism of Choline Oxidase"; Biology Department, Georgia State University, Atlanta, GA, U.S.A.

3. Mahmoud Ghanem, PhD Dissertation: March 2006. Title: "On the Mechanistic Roles of the Protein Positive Charge Close to the N(1) Flavin Locus of Choline Oxidase"; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

4. Osbourne Quaye, PhD Dissertation: August 2009. Title: “On the Pre-Organization of the Active Site of Choline Oxidase for the Hydride Transfer and Tunneling Mechanism”; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

5. Steffan Finnegan, PhD Dissertation: May 2010. Title: “Roles of Serine 101, Histidine 310 and Valine 464 in the Reaction Catalyzed by Choline Oxidase from Arthrobacter globiformis”; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

6. Kunchala Rungsrisuriyachai, PhD Dissertation: May 2010. Title: “On the Catalytic Roles of His351, Asn510 and His466 in Choline Oxidase and the Kinetic Mechanism of Pyranose 2-Oxidase”; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

7. Kevin Francis, PhD Dissertation: May 2011. Title: “On the Biochemistry, Mechanism and Physiological Role of Fungal Nitronate Monooxygenase”; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

8. Hongling Yuan, PhD Dissertation: August 2011. Title: “Mechanistic Studies of Two Selected Flavin-Dependent Enzymes: Choline Oxidase and D-Arginine Dehydrogenase”; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

9. Swathi Gannavaram, PhD Dissertation: June 2014. Title: “Mechanistic Enzymology of Flavin-Dependent Catalysis in Bacterial D-Arginine Dehydrogenase and Choline Oxidase”; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

10. Francesca Salvi, PhD Dissertation: May 2015. Title: “Structural and Kinetic Studies of Flavin0Dependent Enzymes Involved in Glycine Betaine Biosynthesis and Propionate 3-NItronate Detoxification”; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

11. Crystal Smitherman, PhD Dissertation: December 2015. Title: “Biochemical and Mechanistic Studies of Nitronate Monooxygenase and Roles of Histidine Residues in Select Flavoprotein Oxidases”, Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

d.ix) Direction of Individual Student Work (Theses at GSU)

12. Bethany Russell, M.S. Thesis: December 2002. Title: ”Purification and Partial Characterization of Recombinant 2-Nitropopane Dioxygenase from Neurospora crassa”; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.


13. Jane V. Hoang, M.S. Thesis: August 2006. Title: ”Inactivation of Choline Oxidase by Irreversible Inhibitors or Storage Conditions”; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

14. Mijatovic Slavica, M.S. Thesis: May 2008. Title: “Biochemical Characterization of 2-Nitropropane Dioxygenase from Hansenula mrakii”; Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

15. Rizvan Uluisik, M.S. Thesis: May 2013. Title: “Effects of Temperature on the Kinetic Isotope Effects for Proton and Hydride Transfers in the Active Site of Choline Oxidase”, Chemistry Department, Georgia State University, Atlanta, GA, U.S.A.

16. Daniel Ouedraogo, M.S. Thesis: December 2015. Title: “Mechanistic Investigation of the Flavin-Neighboring Residues S45, A46 and I335 in Pseudomonas aeruginosa D-Arginine Dehydrogenase”, Chemistry Department, Georgia State University, Altanta, GA, U.S.A.

d.x) Direction of Individual Student Work (Theses not at GSU)

1. Silvia Zucchelli, Tesi di Laurea in Scienze Biologiche: July 1995. Title “Colesterolo Ossidasi da Streptomyces hygroscopicus”; Dipartimento di Fisiologia e Biochimica Generali, Universita’ degli Studi di Milano, Milano, Italy. Co-Advisor.

2. Giovanni Luca Beretta, Tesi di Laurea in Scienze Biologiche: 1995. Title “Studio del Sito Attivo della D-Amminoacido Ossidasi da Rhodotorula gracilis Mediante Modifica Chimica”; Dipartimento di Fisiologia e Biochimica Generali, Universita’ degli Studi di Milano, Milano, Italy. Co-Advisor.

e) References

Available upon request.

gadda cv (february 15, 2017) - georgia state university · giovanni gadda v.5 2016-2017 guest...

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